TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCS16e03.yg.2.1
(608 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BE404374.1|BE404374 WHE0441_D11_G21ZS Wheat etiolated se... 182 6e-046
gb|BJ269645.1|BJ269645 BJ269645 Y. Ogihara unpublished cDNA... 182 3e-045
gb|CA744678.1|CA744678 wri1s.pk009.k5 wri1s Triticum aestiv... 134 1e-036
gb|CK211085.1|CK211085 FGAS022913 Triticum aestivum FGAS: L... 132 1e-035
gb|BU099724.1|BU099724 WHE3310_G02_M04ZS Chinese Spring whe... 133 2e-034
gb|DR735735.1|DR735735 FGAS081369 Triticum aestivum FGAS: L... 113 1e-029
>gb|BE404374.1|BE404374 WHE0441_D11_G21ZS Wheat etiolated seedling root cDNA library
Triticum aestivum cDNA clone WHE0441_D11_G21, mRNA
sequence
Length = 589
Score = 182 bits (392), Expect = 3e-045
Identities = 76/118 (64%), Positives = 94/118 (79%)
Frame = -2 / +2
Query: 493 EXETGTQSANTLSGYPVANRKRSWENFRVFSRELNSSEMPFAPIASDSISLIISVEDTGV 314
E + + NTLSGY VANR+ SW++F++F+ +L+S+EMPFAPI ++I LIISVEDTGV
Sbjct: 26 EFQNTNNTYNTLSGYKVANRRHSWQSFQLFNTDLHSAEMPFAPILPETIRLIISVEDTGV 205
Query: 313 GIPFDAQSRVFTPFMQVGPSIARIHGGTGIGLSISKCLVGLMRGEIGFASKPQVGSTF 140
GIP +AQSR+F PFMQVGPSIARIHGGTGIGLSISKCLV LM+G +G Q +F
Sbjct: 206 GIPPEAQSRIFMPFMQVGPSIARIHGGTGIGLSISKCLVHLMKGRLGDCKLTQCWFSF 379
Score = 158 bits (340), Expect = 4e-038
Identities = 71/104 (68%), Positives = 78/104 (75%)
Frame = +3 / -2
Query: 156 WGLLANPISPLMSPTKHLLMLNPMPVPPWMRAMDGPTCMKGVKTRD*ASNGIPTPVSSTD 335
W L +P P M T+HLLMLNPMPVPPWMRAMDGPTCMKG+ RD AS G+PTPVSSTD
Sbjct: 363 WVSLQSPNLPFMR*TRHLLMLNPMPVPPWMRAMDGPTCMKGMNIRDWASGGMPTPVSSTD 184
Query: 336 IIKLIESDAMGAKGISDEFSSLLKTRKFSQLRFLFATG*PLKVL 467
II LI S +GAKGIS E S+LK+ K QL L AT *PLKVL
Sbjct: 183 IINLIVSGRIGAKGISAECKSVLKSWKLCQLCLLLATL*PLKVL 52
Score = 109 bits (233), Expect(2) = 6e-046
Identities = 48/95 (50%), Positives = 60/95 (63%)
Frame = -1 / +1
Query: 467 QYLKWLPSGEQKAQLGKLSSL*QGTELI*DAFCTHRIRLNKLDNIS*RYGCWNPVRCSVP 288
Q+L+WL QKA L +L + G L *DA C++ NK+DNI *RY +P +P
Sbjct: 52 QHLEWLQGS*QKA*LAELPAFQYGFTLG*DALCSYPA*NNKIDNIC*RYRRRHPT*SPIP 231
Query: 287 CLHPFHASRSIHCSHPWGHWHWIKHQQMLGWTHEG 183
+H FHA SIHC HPWGH H IKHQQ+ G +HEG
Sbjct: 232 DIHAFHAGWSIHCPHPWGHGHRIKHQQVPGSSHEG 336
Score = 95.5 bits (202), Expect(2) = 6e-046
Identities = 40/57 (70%), Positives = 46/57 (80%)
Frame = -2 / +3
Query: 187 RGEIGFASKPQVGSTFTFTAVLTRAHSSRNENKSSEFKGINALVVDHRPVRAKVTRY 17
RG+ G S P VGS F+FTAVLT+A S+ N NKS FKGINALVVDH+PVRAKVT+Y
Sbjct: 333 RGDWGIVSLPNVGSPFSFTAVLTKARSNANANKSPGFKGINALVVDHKPVRAKVTKY 503
Score = 94.5 bits (200), Expect(2) = 4e-038
Identities = 47/90 (52%), Positives = 54/90 (60%)
Frame = +2 / -1
Query: 188 HESNQAFADA*SNASAPMDASNGWTYLHERGEDTGLSIERDSNTRIFN*YYQAY*V*CDG 367
HE NQA ADA*S+A APMDA NGWT LHER E GL D++ IFN YYQ+Y D
Sbjct: 331 HEMNQALADA*SDARAPMDAGNGWTNLHERHEYPGLGFRWDADAGIFNRYYQSYCFRQDR 152
Query: 368 CKRHLR*VQFPAKDSKVFPTALSVRHWVAT 457
K HL V + + PT SV + VAT
Sbjct: 151 SKGHLSRV*IRIEKLEALPTMPSVSYLVAT 62
Score = 84.5 bits (178), Expect(2) = 4e-038
Identities = 41/57 (71%), Positives = 42/57 (73%)
Frame = +3 / -1
Query: 18 YLVTLARTGL*STTNALIPLNSDDLFSFLLEWALVRTAVKVKVEPTWGLLANPISPL 188
YLVTLARTGL*STTNA IPLN DL +F LE A V TAVK K EPT G L P SPL
Sbjct: 502 YLVTLARTGL*STTNAFIPLNPGDLLAFALERAFVSTAVKEKGEPTLGKLTIPQSPL 332
Score = 71.6 bits (150), Expect(2) = 4e-025
Identities = 33/58 (56%), Positives = 37/58 (63%)
Frame = -1 / +2
Query: 188 EGRDWICK*TPSWFYFHLHCGSYEGPFQQK*E*IVRI*RDQCIGGRS*AGPCQGY*VP 15
+GR CK T WF F LHC +YE Q K I R+*RD+CIG S*AG CQGY VP
Sbjct: 332 KGRLGDCKLTQCWFSFLLHCSAYESTLQCKC*QIARV*RDKCIGS*S*AGSCQGYQVP 505
Score = 63.4 bits (132), Expect(2) = 4e-025
Identities = 39/68 (57%), Positives = 42/68 (61%)
Frame = -3 / +3
Query: 390 THLRCLLHPSHQTQ*A**YQLKIRVLESRSMLSPVSSPLSCK*VHPLLASMGALALD*AS 211
T LRC L S Q* **Y LKI S +P S LSC+ VHPL ASMGA A D*AS
Sbjct: 129 TRLRCPLLLSCLKQ*D**YLLKIPASASHLKPNPGYSCLSCRLVHPLPASMGARASD*AS 308
Query: 210 ANAWLDS* 187
A+AW S*
Sbjct: 309 ASAWFIS* 332
Score = 62.5 bits (130), Expect(3) = 2e-024
Identities = 26/47 (55%), Positives = 30/47 (63%)
Frame = +1 / -3
Query: 184 PS*VQPSIC*CLIQCQCPHGCEQWMDLLA*KG*RHGTEHRTGFQHPY 324
PS* +P C*CLI+C CPHGC QWMD A*K * G + G + Y
Sbjct: 335 PS*DEPGTC*CLIRCPCPHGCGQWMDQPA*KA*ISGIGLQVGCRRRY 195
Score = 62.5 bits (130), Expect(3) = 2e-024
Identities = 31/62 (50%), Positives = 38/62 (61%)
Frame = +2 / -3
Query: 2 RPPGQVPSNLGTDRPMIYHQCIDPFKF*RFILISAGMGPRKNRSEGESRTNLGFTCKSNL 181
+P V NLGT+R MI +QCI PFK RF+ I G RK+ SEGE RTN+G +
Sbjct: 518 KPLQMVLGNLGTNRLMINYQCIYPFKPWRFVSICIGACFRKHCSEGERRTNIG*AYNPPI 339
Query: 182 SP 187
SP
Sbjct: 338 SP 333
Score = 25.4 bits (49), Expect(3) = 2e-024
Identities = 16/34 (47%), Positives = 18/34 (52%)
Frame = +1 / -3
Query: 370 QKASQMSSVPC*RLESFPNCAFCSPLGSHLRYWR 471
Q+ASQ S P *+ S N AFC SH R R
Sbjct: 149 QRASQPSVNPY*KAGSSANYAFC*LPCSHSRCCR 48
>gb|BJ269645.1|BJ269645 BJ269645 Y. Ogihara unpublished cDNA library, Wh_oh Triticum
aestivum cDNA clone whoh22o12 5', mRNA sequence
Length = 664
Score = 182 bits (392), Expect = 3e-045
Identities = 75/106 (70%), Positives = 91/106 (85%)
Frame = -2 / +2
Query: 493 EXETGTQSANTLSGYPVANRKRSWENFRVFSRELNSSEMPFAPIASDSISLIISVEDTGV 314
E + + NTLSGY VANR+ SW++F++F+ +L+S+EMPFAPI ++I LIISVEDTGV
Sbjct: 347 EFQNTNNTYNTLSGYKVANRRHSWQSFQLFNTDLHSAEMPFAPILPETIRLIISVEDTGV 526
Query: 313 GIPFDAQSRVFTPFMQVGPSIARIHGGTGIGLSISKCLVGLMRGEI 176
GIP +AQSR+F PFMQVGPSIARIHGGTGIGLSISKCLV LM+GEI
Sbjct: 527 GIPPEAQSRIFMPFMQVGPSIARIHGGTGIGLSISKCLVHLMKGEI 664
Score = 156 bits (336), Expect(2) = 3e-041
Identities = 70/97 (72%), Positives = 76/97 (78%)
Frame = +3 / -2
Query: 177 ISPLMSPTKHLLMLNPMPVPPWMRAMDGPTCMKGVKTRD*ASNGIPTPVSSTDIIKLIES 356
ISP M T+HLLMLNPMPVPPWMRAMDGPTCMKG+ RD AS G+PTPVSSTDII LI S
Sbjct: 663 ISPFMR*TRHLLMLNPMPVPPWMRAMDGPTCMKGMNIRDWASGGMPTPVSSTDIINLIVS 484
Query: 357 DAMGAKGISDEFSSLLKTRKFSQLRFLFATG*PLKVL 467
+GAKGIS E S+LK+ K QL L AT *PLKVL
Sbjct: 483 GRIGAKGISAECKSVLKSWKLCQLCLLLATL*PLKVL 373
Score = 111 bits (237), Expect(2) = 4e-032
Identities = 49/97 (50%), Positives = 61/97 (62%)
Frame = -1 / +1
Query: 467 QYLKWLPSGEQKAQLGKLSSL*QGTELI*DAFCTHRIRLNKLDNIS*RYGCWNPVRCSVP 288
Q+L+WL QKA L +L + G L *DA C++ NK+DNI *RY +P +P
Sbjct: 373 QHLEWLQGS*QKA*LAELPAFQYGFTLG*DALCSYPA*NNKIDNIC*RYRRRHPT*SPIP 552
Query: 287 CLHPFHASRSIHCSHPWGHWHWIKHQQMLGWTHEGRD 177
+H FHA SIHC HPWGH H IKHQQ+ G +HEG D
Sbjct: 553 DIHAFHAGWSIHCPHPWGHGHRIKHQQVPGSSHEGGD 663
Score = 47.3 bits (97), Expect(2) = 4e-032
Identities = 22/43 (51%), Positives = 24/43 (55%)
Frame = -2 / +2
Query: 592 QIXXXXXXXSXXXTEXXHIYXTVHVVEEIMXXLEXETGTQSAN 464
QI S TE HIY TVHVVEE+M LE ET Q+ N
Sbjct: 236 QIITNLMGNSIKFTERGHIYLTVHVVEEVMSCLEVETEFQNTN 364
Score = 32.7 bits (65), Expect(2) = 3e-041
Identities = 10/19 (52%), Positives = 14/19 (73%)
Frame = +2 / -1
Query: 497 TXHNLFNNMNCXVDMXXLC 553
T H+LF++MNC VD+ C
Sbjct: 331 TTHDLFDDMNCQVDVSSFC 275
>gb|CA744678.1|CA744678 wri1s.pk009.k5 wri1s Triticum aestivum cDNA clone wri1s.pk009.k5 5'
end, mRNA sequence
Length = 707
Score = 134 bits (287), Expect(3) = 1e-036
Identities = 55/81 (67%), Positives = 66/81 (81%)
Frame = -2 / -2
Query: 358 SDSISLIISVEDTGVGIPFDAQSRVFTPFMQVGPSIARIHGGTGIGLSISKCLVGLMRGE 179
S++++L++SVEDTG+GIP AQ RVF PFMQ S +R +GGTGIGLSISKCLV LM G+
Sbjct: 550 SENVTLVVSVEDTGIGIPIHAQGRVFMPFMQADSSTSRNYGGTGIGLSISKCLVELMSGQ 371
Query: 178 IGFASKPQVGSTFTFTAVLTR 116
I F S+P VGSTFTFTAVL R
Sbjct: 370 INFVSRPNVGSTFTFTAVLQR 308
Score = 34.5 bits (69), Expect(3) = 1e-036
Identities = 13/23 (56%), Positives = 17/23 (73%)
Frame = -2 / -2
Query: 85 SEFKGINALVVDHRPVRAKVTRY 17
S F+G++ L+VD RPVRA V Y
Sbjct: 253 SSFQGLSTLLVDKRPVRATVAEY 185
Score = 23.1 bits (44), Expect(3) = 1e-036
Identities = 7/14 (50%), Positives = 11/14 (78%)
Frame = -2 / -3
Query: 442 ANRKRSWENFRVFS 401
A+ + SWENF+ F+
Sbjct: 630 ADSRNSWENFKGFA 589
>gb|CK211085.1|CK211085 FGAS022913 Triticum aestivum FGAS: Library 5 GATE 7 Triticum
aestivum cDNA, mRNA sequence
Length = 1144
Score = 132 bits (282), Expect(3) = 1e-035
Identities = 54/81 (66%), Positives = 66/81 (81%)
Frame = -2 / +1
Query: 358 SDSISLIISVEDTGVGIPFDAQSRVFTPFMQVGPSIARIHGGTGIGLSISKCLVGLMRGE 179
S++++L++SV+DTG+GIP AQ RVF PFMQ S +R +GGTGIGLSISKCLV LM G+
Sbjct: 436 SENVTLVVSVDDTGIGIPIHAQGRVFMPFMQAHRSTSRNYGGTGIGLSISKCLVELMSGQ 615
Query: 178 IGFASKPQVGSTFTFTAVLTR 116
I F S+P VGSTFTFTAVL R
Sbjct: 616 INFVSRPNVGSTFTFTAVLQR 678
Score = 29.0 bits (57), Expect(3) = 1e-035
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = -2 / +1
Query: 472 SANTLSGYPVANRKRSWENFR 410
S TLSG+ A+ + SWE F+
Sbjct: 328 SFTTLSGFEAADTRNSWETFK 390
Score = 27.6 bits (54), Expect(3) = 1e-035
Identities = 12/23 (52%), Positives = 16/23 (69%)
Frame = -2 / +1
Query: 85 SEFKGINALVVDHRPVRAKVTRY 17
S F+G++ L+ RPVRA VT Y
Sbjct: 733 SSFQGLSTLLG**RPVRATVTEY 801
>gb|BU099724.1|BU099724 WHE3310_G02_M04ZS Chinese Spring wheat drought stressed root cDNA
library Triticum aestivum cDNA clone WHE3310_G02_M04,
mRNA sequence
Length = 667
Score = 133 bits (284), Expect(2) = 2e-034
Identities = 55/81 (67%), Positives = 65/81 (80%)
Frame = -2 / +2
Query: 358 SDSISLIISVEDTGVGIPFDAQSRVFTPFMQVGPSIARIHGGTGIGLSISKCLVGLMRGE 179
S +++L++SVEDTG+GIP AQ RVF PFMQ S +R +GGTGIGLSISKCLV LM G+
Sbjct: 335 SGNVTLVVSVEDTGIGIPIHAQGRVFMPFMQADSSTSRNYGGTGIGLSISKCLVELMSGQ 514
Query: 178 IGFASKPQVGSTFTFTAVLTR 116
I F S+P VGSTFTFTAVL R
Sbjct: 515 INFVSRPNVGSTFTFTAVLQR 577
Score = 33.6 bits (67), Expect(2) = 2e-034
Identities = 11/19 (57%), Positives = 15/19 (78%)
Frame = -2 / +2
Query: 466 NTLSGYPVANRKRSWENFR 410
NTLSG+ A+ + SWENF+
Sbjct: 233 NTLSGFEAADSRNSWENFK 289
>gb|DR735735.1|DR735735 FGAS081369 Triticum aestivum FGAS: Library 4 Gate 8 Triticum
aestivum cDNA, mRNA sequence
Length = 1109
Score = 113 bits (242), Expect(2) = 1e-029
Identities = 48/68 (70%), Positives = 53/68 (77%)
Frame = -2 / +1
Query: 319 GVGIPFDAQSRVFTPFMQVGPSIARIHGGTGIGLSISKCLVGLMRGEIGFASKPQVGSTF 140
G+GIP AQ RVF PFMQ S +R +GGTGIGLSISKCLV LM G+I F S+P VGSTF
Sbjct: 22 GIGIPIHAQGRVFMPFMQADSSTSRNYGGTGIGLSISKCLVELMSGQINFVSRPNVGSTF 201
Query: 139 TFTAVLTR 116
TFTAVL R
Sbjct: 202 TFTAVLQR 225
Score = 36.8 bits (74), Expect(2) = 1e-029
Identities = 14/23 (60%), Positives = 18/23 (78%)
Frame = -2 / +1
Query: 85 SEFKGINALVVDHRPVRAKVTRY 17
S F+G++ L+VD RPVRA VT Y
Sbjct: 280 SSFQGLSTLLVDKRPVRATVTEY 348
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 536,992,788
Number of Sequences: 636343
Number of extensions: 8455637
Number of successful extensions: 124452
Number of sequences better than 1.0e-020: 9
length of database: 122,413,413
effective HSP length: 53
effective length of database: 88,687,234
effective search space used: 13214397866
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)