TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCN28g01.yg.2.1
(513 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA723856.1|CA723856 wds1f.pk002.o9 wds1f Triticum aestiv... 274 6e-073
gb|CA724387.1|CA724387 wds1f.pk004.o9 wds1f Triticum aestiv... 176 1e-059
gb|CK206579.1|CK206579 FGAS018178 Triticum aestivum FGAS: L... 100 2e-039
gb|BE428381.1|BE428381 MTD006.D05F990616 ITEC MTD Durum Whe... 96 9e-028
gb|AL830216.1|AL830216 AL830216 q:343 Triticum aestivum cDN... 87 3e-027
>gb|CA723856.1|CA723856 wds1f.pk002.o9 wds1f Triticum aestivum cDNA clone wds1f.pk002.o9 5'
end, mRNA sequence
Length = 426
Score = 274 bits (592), Expect = 6e-073
Identities = 112/128 (87%), Positives = 121/128 (94%)
Frame = -1 / +2
Query: 483 LTVKESAMDVPTNLDARRRISFFANSLFMSMPDAPKVRQMLPFSVLTPYYKEDVLFSSQA 304
LTVKESAMDVPTNLDARRRISFFANSLFM MP APKVR MLPFSVLTPYYKEDVLFSSQA
Sbjct: 2 LTVKESAMDVPTNLDARRRISFFANSLFMDMPKAPKVRNMLPFSVLTPYYKEDVLFSSQA 181
Query: 303 LAEQNEDGVSILFYLQKIYPDEWKNFLERVHCESEDQLHETEHSEEQLRLWASYRGQTLT 124
L E+NEDGVSILFYLQKIYPDEWKNFLERV C++E++L ETE +E++LRLWASY+GQTLT
Sbjct: 182 LEEENEDGVSILFYLQKIYPDEWKNFLERVDCKNEEELRETEQTEDELRLWASYKGQTLT 361
Query: 123 RTVRGMMY 100
RTVRGMMY
Sbjct: 362 RTVRGMMY 385
Score = 253 bits (546), Expect = 1e-066
Identities = 98/129 (75%), Positives = 113/129 (87%)
Frame = +1 / -3
Query: 97 LVHHPSYSSCQSLSPV*CPKAKLLFRMLSLVELIFTLTMHPFKKIFPFIWVDFLQIKKDG 276
+VHHPSYSS QSL+ V*CPKAKL+FR+ SL E +F L +HPFKK+FPFIWVDFLQ+KKD
Sbjct: 388 VVHHPSYSSRQSLALV*CPKAKLIFRLFSLTEFLFILAVHPFKKVFPFIWVDFLQVKKDR 209
Query: 277 NSVLILFC*CL*REKNIFFIIRSQDREGQHLPHFWSIWHRHKKRISKERYPPSSIQVCRD 456
N +L+LF *CL* EK++FF+I SQDREGQH+ HFWS WH HKKRISKERYPPSSIQVCR+
Sbjct: 208 NPILVLFL*CL*GEKDVFFVIGSQDREGQHIAHFWSFWHVHKKRISKERYPPSSIQVCRN 29
Query: 457 IHSRFLHRE 483
IHSRFLH E
Sbjct: 28 IHSRFLHCE 2
Score = 210 bits (452), Expect = 1e-053
Identities = 96/128 (75%), Positives = 101/128 (78%)
Frame = +2 / -1
Query: 101 YIIPLTVLVKVCPRYDAQRRXXXXXXXXXXXXXXXXXXXXXRKFFHSSG*IFCK*KRMET 280
YIIPLTVLVKV P YDAQRR RKFFHSSG*IFC+*KR+ET
Sbjct: 384 YIIPLTVLVKVWPLYDAQRRSSSSVCSVSRSSSSFLQSTLSRKFFHSSG*IFCR*KRIET 205
Query: 281 PSSFCSASACEEKRTSSL**GVKTEKGNICRTFGASGIDIKRELAKKDILLLASRFVGTS 460
PSSF S+SACEEKRTSSL**GVKTEKG+I RTFGA G+ IKRELAKKDILLLASRFVGTS
Sbjct: 204 PSSFSSSSACEEKRTSSL**GVKTEKGSILRTFGAFGMSIKRELAKKDILLLASRFVGTS 25
Query: 461 IADSFTVS 484
IADSFTVS
Sbjct: 24 IADSFTVS 1
Score = 208 bits (448), Expect = 5e-053
Identities = 95/132 (71%), Positives = 100/132 (75%)
Frame = -2 / +3
Query: 482 SR*RNRLWMSLQTWMLEGGYLSLLILFLCRCQMLQKCGKCCPSLS*LLIIKKMFFSLHKH 303
S+*RN LWM LQTWMLEGGYLSLLILFL CQ LQKC CCPSLS*L I KK FS HKH
Sbjct: 3 SQ*RNLLWMFLQTWMLEGGYLSLLILFLWTCQKLQKCAICCPSLS*LPITKKTSFSPHKH 182
Query: 302 *QNRMRTEFPSFFICKKSTQMNGKIFLKGCIVRVKINSTRLSILKSSFAFGHHTGDKL*Q 123
*+ R R F SFF CKKSTQMNGK FLKG R+K NS RL+ K SFAFGHHT KL*+
Sbjct: 183 *RKRTRMGFLSFFTCKKSTQMNGKTFLKGWTARMKRNSVRLNKRKMSFAFGHHTRAKL*R 362
Query: 122 EL*EG*CTKKDL 87
EL*EG*CT L
Sbjct: 363 EL*EG*CTTDKL 398
Score = 167 bits (360), Expect = 6e-041
Identities = 81/112 (72%), Positives = 87/112 (77%)
Frame = +3 / -2
Query: 102 TSSLLQFLSKFVPGMMPKGEAALQNAQSRGVDLHSHNAPFQENFSIHLGRFFANKKGWKL 281
TSSLLQF SKF P MMPKGEA L QS GV LHS + PFQE+FSIHLGRFFA KKG K
Sbjct: 383 TSSLLQFSSKFGPCMMPKGEAHLPFVQSHGVPLHSCSPPFQESFSIHLGRFFAGKKG*KP 204
Query: 282 RPHSVLLVLVKRKEHLLYNKESRQRRATFAALLEHLAST*KEN*QRKISSF* 437
P S+ LVLV+RK LL N+ESRQRRA + ALLE LA *KEN*QRKISSF*
Sbjct: 203 HPRSLPLVLVRRKGRLLCNRESRQRRAAYCALLELLACP*KEN*QRKISSF* 48
Score = 101 bits (215), Expect(2) = 1e-037
Identities = 50/70 (71%), Positives = 52/70 (74%)
Frame = -3 / +1
Query: 310 TSTSRTE*GRSFHPFLFAKNLPR*MEKFS*KGAL*E*RSTPRD*AF*RAASPLGIIPGTN 131
TST E G F+PFL AKNLPR*MEK S*KG L E*R TP D* R ASPLGII G N
Sbjct: 175 TSTRGRERGWGFYPFLPAKNLPR*MEKLS*KGGLQE*RGTP*D*TNGR*ASPLGIIQGPN 354
Query: 130 FDKNCKRDDV 101
FD+NCKRDDV
Sbjct: 355 FDENCKRDDV 384
Score = 75.3 bits (158), Expect(2) = 1e-037
Identities = 31/41 (75%), Positives = 32/41 (78%)
Frame = -3 / +1
Query: 481 HGEGIGYGCPYKPGC*KEDIFLC*FSFYVDARCSKSAANVA 359
H EGI YGC YKPGC*KEDIFLC*FSFY A+ SKSA A
Sbjct: 4 HSEGICYGCSYKPGC*KEDIFLC*FSFYGHAKSSKSAQYAA 126
>gb|CA724387.1|CA724387 wds1f.pk004.o9 wds1f Triticum aestivum cDNA clone wds1f.pk004.o9 5'
end, mRNA sequence
Length = 430
Score = 176 bits (379), Expect(3) = 1e-059
Identities = 75/80 (93%), Positives = 76/80 (95%)
Frame = -1 / +2
Query: 483 LTVKESAMDVPTNLDARRRISFFANSLFMSMPDAPKVRQMLPFSVLTPYYKEDVLFSSQA 304
LTVKESAMDVPTNLDARRRISFFANSLFM MP APKVR MLPFSVLTPYYKEDVLFSSQA
Sbjct: 2 LTVKESAMDVPTNLDARRRISFFANSLFMDMPKAPKVRNMLPFSVLTPYYKEDVLFSSQA 181
Query: 303 LAEQNEDGVSILFYLQKIYP 244
L E+NEDGVSILFYLQKIYP
Sbjct: 182 LEEENEDGVSILFYLQKIYP 241
Score = 168 bits (362), Expect(2) = 8e-048
Identities = 65/87 (74%), Positives = 74/87 (85%)
Frame = +1 / -1
Query: 223 KKIFPFIWVDFLQIKKDGNSVLILFC*CL*REKNIFFIIRSQDREGQHLPHFWSIWHRHK 402
+K F WVDFLQ+KKD N +L+LF *CL* EK++FF+I SQDREGQH+ HFWS WH HK
Sbjct: 262 RKFFHSSWVDFLQVKKDRNPILVLFL*CL*GEKDVFFVIGSQDREGQHIAHFWSFWHVHK 83
Query: 403 KRISKERYPPSSIQVCRDIHSRFLHRE 483
KRISKERYPPSSIQVCR+IHSRFLH E
Sbjct: 82 KRISKERYPPSSIQVCRNIHSRFLHCE 2
Score = 156 bits (336), Expect(3) = 3e-056
Identities = 71/80 (88%), Positives = 76/80 (95%)
Frame = +2 / -2
Query: 245 G*IFCK*KRMETPSSFCSASACEEKRTSSL**GVKTEKGNICRTFGASGIDIKRELAKKD 424
G*IFC+*KR+ETPSSF S+SACEEKRTSSL**GVKTEKG+I RTFGA G+ IKRELAKKD
Sbjct: 240 G*IFCR*KRIETPSSFSSSSACEEKRTSSL**GVKTEKGSILRTFGAFGMSIKRELAKKD 61
Query: 425 ILLLASRFVGTSIADSFTVS 484
ILLLASRFVGTSIADSFTVS
Sbjct: 60 ILLLASRFVGTSIADSFTVS 1
Score = 139 bits (299), Expect(2) = 4e-041
Identities = 63/88 (71%), Positives = 66/88 (75%)
Frame = -2 / +3
Query: 482 SR*RNRLWMSLQTWMLEGGYLSLLILFLCRCQMLQKCGKCCPSLS*LLIIKKMFFSLHKH 303
S+*RN LWM LQTWMLEGGYLSLLILFL CQ LQKC CCPSLS*L I KK FS HKH
Sbjct: 3 SQ*RNLLWMFLQTWMLEGGYLSLLILFLWTCQKLQKCAICCPSLS*LPITKKTSFSPHKH 182
Query: 302 *QNRMRTEFPSFFICKKSTQMNGKIFLK 219
*+ R R F SFF CKKSTQ K FL+
Sbjct: 183 *RKRTRMGFLSFFTCKKSTQDEWKNFLE 266
Score = 101 bits (216), Expect(2) = 1e-027
Identities = 50/74 (67%), Positives = 56/74 (75%)
Frame = +3 / -3
Query: 216 PFQENFSIHLGRFFANKKGWKLRPHSVLLVLVKRKEHLLYNKESRQRRATFAALLEHLAS 395
PF++ F LGRFFA KKG K P S+ LVLV+RK LL N+ESRQRRA + ALLE LA
Sbjct: 269 PFKKVFPFILGRFFAGKKG*KPHPRSLPLVLVRRKGRLLCNRESRQRRAAYCALLELLAC 90
Query: 396 T*KEN*QRKISSF* 437
*KEN*QRKISSF*
Sbjct: 89 P*KEN*QRKISSF* 48
Score = 75.3 bits (158), Expect(3) = 6e-032
Identities = 31/41 (75%), Positives = 32/41 (78%)
Frame = -3 / +1
Query: 481 HGEGIGYGCPYKPGC*KEDIFLC*FSFYVDARCSKSAANVA 359
H EGI YGC YKPGC*KEDIFLC*FSFY A+ SKSA A
Sbjct: 4 HSEGICYGCSYKPGC*KEDIFLC*FSFYGHAKSSKSAQYAA 126
Score = 54.7 bits (113), Expect(3) = 1e-059
Identities = 20/24 (83%), Positives = 24/24 (100%)
Frame = -1 / +1
Query: 171 EEQLRLWASYRGQTLTRTVRGMMY 100
+++LRLWASYRGQTLT+TVRGMMY
Sbjct: 316 KDELRLWASYRGQTLTKTVRGMMY 387
Score = 51.5 bits (106), Expect(3) = 6e-032
Identities = 20/38 (52%), Positives = 26/38 (68%)
Frame = -1 / +3
Query: 282 GVSILFYLQKIYPDEWKNFLERVHCESEDQLHETEHSE 169
G F +K DEWKNFLERV C++E++L ETE +E
Sbjct: 204 GFLSFFTCKKSTQDEWKNFLERVDCKNEEELRETEQTE 317
Score = 51.5 bits (106), Expect(3) = 3e-056
Identities = 20/25 (80%), Positives = 23/25 (92%)
Frame = +1 / -2
Query: 97 LVHHPSYSSCQSLSPV*CPKAKLLF 171
+VHHPSYS QSL+PV*CPKAKL+F
Sbjct: 390 VVHHPSYSFRQSLAPV*CPKAKLIF 316
Score = 48.3 bits (99), Expect(2) = 4e-041
Identities = 21/28 (75%), Positives = 23/28 (82%)
Frame = -2 / +2
Query: 170 KSSFAFGHHTGDKL*QEL*EG*CTKKDL 87
K SFAFGHHTG KL*++L*EG*CT L
Sbjct: 317 KMSFAFGHHTGAKL*RKL*EG*CTTDKL 400
Score = 48.3 bits (99), Expect(3) = 3e-056
Identities = 19/34 (55%), Positives = 23/34 (67%)
Frame = +1 / -3
Query: 169 FRMLSLVELIFTLTMHPFKKIFPFIWVDFLQIKK 270
FR+ SL E +F L +HPFKK+FPFI F KK
Sbjct: 317 FRLFSLTEFLFILAVHPFKKVFPFILGRFFAGKK 216
Score = 47.8 bits (98), Expect(3) = 6e-032
Identities = 19/23 (82%), Positives = 20/23 (86%)
Frame = -3 / +3
Query: 169 RAASPLGIIPGTNFDKNCKRDDV 101
R ASPLGII G NFD+NCKRDDV
Sbjct: 318 R*ASPLGIIQGPNFDENCKRDDV 386
Score = 41.8 bits (85), Expect(2) = 8e-048
Identities = 19/23 (82%), Positives = 19/23 (82%)
Frame = +3 / -1
Query: 102 TSSLLQFLSKFVPGMMPKGEAAL 170
TSSLLQF SKF P MMPKGEA L
Sbjct: 385 TSSLLQFSSKFGPCMMPKGEAHL 317
Score = 40.9 bits (83), Expect(2) = 1e-027
Identities = 17/20 (85%), Positives = 17/20 (85%)
Frame = +2 / -3
Query: 101 YIIPLTVLVKVCPRYDAQRR 160
YIIPLTV VKV P YDAQRR
Sbjct: 386 YIIPLTVFVKVWPLYDAQRR 327
Score = 37.3 bits (75), Expect(3) = 1e-059
Identities = 20/30 (66%), Positives = 20/30 (66%)
Frame = -3 / +2
Query: 268 FLFAKNLPR*MEKFS*KGAL*E*RSTPRD* 179
F K PR*MEK S*KG L E*R TP D*
Sbjct: 218 FYLQKIYPR*MEKLS*KGGLQE*RGTP*D* 307
>gb|CK206579.1|CK206579 FGAS018178 Triticum aestivum FGAS: Library 5 GATE 7 Triticum
aestivum cDNA, mRNA sequence
Length = 1115
Score = 100 bits (213), Expect(3) = 2e-039
Identities = 42/71 (59%), Positives = 52/71 (73%)
Frame = -1 / +3
Query: 495 LRLLLTVKESAMDVPTNLDARRRISFFANSLFMSMPDAPKVRQMLPFSVLTPYYKEDVLF 316
L LLL KE A +VP+N +ARRRISFFA SLFM MP APKVR ML FS++TPY+ E+V F
Sbjct: 177 LYLLLNTKEKAAEVPSNSEARRRISFFATSLFMDMPAAPKVRSMLSFSIVTPYFMEEVKF 356
Query: 315 SSQALAEQNED 283
S + L ++
Sbjct: 357 SDEELHSDQDE 389
Score = 51.0 bits (105), Expect(3) = 2e-039
Identities = 19/31 (61%), Positives = 24/31 (77%)
Frame = -1 / +3
Query: 183 TEHSEEQLRLWASYRGQTLTRTVRGMMYQER 91
T+ E +R WAS+RGQTL+RTVRGMMY +
Sbjct: 459 TKVKSEDIRYWASFRGQTLSRTVRGMMYYRK 551
Score = 49.6 bits (102), Expect(3) = 2e-039
Identities = 18/26 (69%), Positives = 22/26 (84%)
Frame = -1 / +3
Query: 291 NEDGVSILFYLQKIYPDEWKNFLERV 214
++D SIL Y+QKIYPDEW NFLER+
Sbjct: 378 DQDEASILSYMQKIYPDEWTNFLERL 455
>gb|BE428381.1|BE428381 MTD006.D05F990616 ITEC MTD Durum Wheat Root Library Triticum
turgidum subsp. durum cDNA clone MTD006.D05, mRNA
sequence
Length = 396
Score = 95.9 bits (203), Expect(2) = 9e-028
Identities = 35/55 (63%), Positives = 46/55 (83%)
Frame = -1 / +3
Query: 357 FSVLTPYYKEDVLFSSQALAEQNEDGVSILFYLQKIYPDEWKNFLERVHCESEDQ 193
+ VLTPYY E+VL+SS L +NEDG+SILFYLQKIYPDEWKNFL+R+ + +++
Sbjct: 48 YCVLTPYYNEEVLYSSHQLNRKNEDGISILFYLQKIYPDEWKNFLQRIGVDPDNE 212
Score = 47.3 bits (97), Expect(2) = 9e-028
Identities = 18/21 (85%), Positives = 18/21 (85%)
Frame = -1 / +1
Query: 153 WASYRGQTLTRTVRGMMYQER 91
WASYRGQTL RTVRGMMY R
Sbjct: 250 WASYRGQTLARTVRGMMYYRR 312
>gb|AL830216.1|AL830216 AL830216 q:343 Triticum aestivum cDNA clone A06_q343_plate_2, mRNA
sequence
Length = 570
Score = 87.2 bits (184), Expect(2) = 3e-027
Identities = 33/54 (61%), Positives = 42/54 (77%)
Frame = -1 / +3
Query: 372 RQMLPFSVLTPYYKEDVLFSSQALAEQNEDGVSILFYLQKIYPDEWKNFLERVH 211
++ LP +V TPYY E VL+S L ++NEDG+S L YLQKIYPDEWKNFL R++
Sbjct: 27 QKCLPLAVFTPYYSETVLYSIAELQKKNEDGISTLCYLQKIYPDEWKNFLTRIN 188
Score = 54.7 bits (113), Expect(2) = 3e-027
Identities = 22/45 (48%), Positives = 30/45 (66%)
Frame = -1 / +3
Query: 225 LERVHCESEDQLHETEHSEEQLRLWASYRGQTLTRTVRGMMYQER 91
+ R ++ +L + + +LRLWASYRGQTL RTVRGMMY +
Sbjct: 183 INRYENAADTELFSSANDILELRLWASYRGQTLARTVRGMMYYRK 317
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 462,575,242
Number of Sequences: 636343
Number of extensions: 6407774
Number of successful extensions: 119791
Number of sequences better than 1.0e-020: 6
length of database: 122,413,413
effective HSP length: 52
effective length of database: 89,323,577
effective search space used: 10540182086
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)