TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCH10g11.yg.2.1
(632 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA497978.1|CA497978 WHE3236_G04_N08ZT Wheat meiotic anth... 295 1e-080
gb|CA501322.1|CA501322 WHE4032_B11_D22ZT Wheat meiotic anth... 272 3e-079
gb|BQ902766.1|BQ902766 Ta03_01c07_R Ta03_AAFC_ECORC_Fusariu... 230 1e-059
gb|BU099331.1|BU099331 WHE3306_C03_E06ZS Chinese Spring whe... 190 9e-057
gb|BE498502.1|BE498502 WHE0967_A12_A23ZS Wheat pre-anthesis... 167 7e-048
gb|DQ086486.1| Triticum aestivum putative 1,3-beta-glucan s... 156 2e-046
gb|BQ743211.1|BQ743211 WHE4101_D09_G17ZS Wheat salt-stresse... 113 1e-033
gb|CF133118.1|CF133118 WHE4354_C05_F10ZT Wheat meiotic flor... 118 6e-026
>gb|CA497978.1|CA497978 WHE3236_G04_N08ZT Wheat meiotic anther cDNA library Triticum
aestivum cDNA clone WHE3236_G04_N08, mRNA sequence
Length = 633
Score = 295 bits (639), Expect(2) = 1e-080
Identities = 124/150 (82%), Positives = 138/150 (92%)
Frame = -3 / +3
Query: 453 REVEIYRVKLPGELKIGEGKPENQNHALIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEF 274
++ EIY VKLPG K+GEGKPENQNHA+IFTRG+AVQTIDMNQDNYFEEALKMRNLLEEF
Sbjct: 135 KDKEIYSVKLPGNPKLGEGKPENQNHAVIFTRGNAVQTIDMNQDNYFEEALKMRNLLEEF 314
Query: 273 NRYYGIRKPKILGVREHVFTGSVSSLAWFMSAQETSFVTLGQRVLADPLKVRMHYGHPDV 94
++ +G +P ILGVREHVFTGSVSSLA FMS QETSFVTLGQRVL++PLKVRMHYGHPDV
Sbjct: 315 SQNHGKFRPSILGVREHVFTGSVSSLASFMSNQETSFVTLGQRVLSNPLKVRMHYGHPDV 494
Query: 93 FDRLWFLGRGGISKASRIINISEDIFAGFN 4
FDR++ + RGGISKASRIINISEDIFAGFN
Sbjct: 495 FDRIFHITRGGISKASRIINISEDIFAGFN 584
Score = 107 bits (228), Expect = 1e-022
Identities = 55/147 (37%), Positives = 81/147 (55%)
Frame = +1 / -2
Query: 4 VETSKNVLTNVDYS*CFTDTTSAQKPKTIKNIWVAIMHSDFKWISQNTLPQSDKTCLLGR 183
V+ K +L ++D + T T +IK++WV IMHS F+WI +NTL KT L
Sbjct: 584 VKPCKYILADIDDTGRLTYATPRNMKYSIKHVWVTIMHSHFQWIRKNTLS*CHKTSFLI* 405
Query: 184 HEPSKRRN*TREDVFPNPKNFRLANSIITIELF**VSHL*SFFKVVVLVHINSLNCIAPC 363
H+ SK N + ED+F N KN R ++I E +SH ++V+L+HIN L I+
Sbjct: 404 HKRSKGGNTSGEDMFSNTKN*RPELAMILREFLKQISHFECLLEIVILIHINGLYGISAS 225
Query: 364 EDECMILIFWLSFANLQLSRQLNPIDF 444
E M+LIF + L ++ Q N I+F
Sbjct: 224 EYYSMVLIFGFTLPKLWIAGQFNRINF 144
Score = 83.5 bits (176), Expect(3) = 4e-028
Identities = 37/49 (75%), Positives = 39/49 (79%)
Frame = +2 / -3
Query: 89 SKTSGWP*CILTLSGSARTRCPRVTKLVSWADMNQAREETEPVKTCSRT 235
S TSG P*CILT SG RTRCP VTKLVS D+N+AREET PVKTCS T
Sbjct: 499 SNTSG*P*CILTFSGLERTRCPNVTKLVS*FDINEAREETLPVKTCSLT 353
Score = 44.1 bits (90), Expect(3) = 4e-028
Identities = 23/32 (71%), Positives = 25/32 (78%)
Frame = +2 / -3
Query: 275 NSSSRFLIFRASSK*LSWFISIV*TASPLVKM 370
NSSSRFLI ASSK*LS FIS+V TA P V +
Sbjct: 313 NSSSRFLILSASSK*LS*FISMVCTAFPRVNI 218
Score = 35.4 bits (71), Expect(3) = 4e-028
Identities = 14/22 (63%), Positives = 19/22 (86%)
Frame = +2 / -3
Query: 2 QLKPAKMSSLMLIILDALLIPP 67
+L PA +SSL+L+I DALL+PP
Sbjct: 586 ELNPANISSLILMIRDALLMPP 521
Score = 25.4 bits (49), Expect(2) = 1e-080
Identities = 9/14 (64%), Positives = 12/14 (85%)
Frame = -3 / +3
Query: 564 LMKNYEALRVAYVD 523
LM+ EALR+AY+D
Sbjct: 18 LMQRNEALRIAYID 59
>gb|CA501322.1|CA501322 WHE4032_B11_D22ZT Wheat meiotic anther cDNA library Triticum
aestivum cDNA clone WHE4032_B11_D22, mRNA sequence
Length = 749
Score = 272 bits (588), Expect(2) = 3e-079
Identities = 114/140 (81%), Positives = 128/140 (91%)
Frame = -3 / +1
Query: 453 REVEIYRVKLPGELKIGEGKPENQNHALIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEF 274
++ EIY VKLPG K+GEGKPENQNHA+IFTRG+AVQTIDMNQDNYFEEALKMRNLLEEF
Sbjct: 328 KDKEIYSVKLPGNPKLGEGKPENQNHAVIFTRGNAVQTIDMNQDNYFEEALKMRNLLEEF 507
Query: 273 NRYYGIRKPKILGVREHVFTGSVSSLAWFMSAQETSFVTLGQRVLADPLKVRMHYGHPDV 94
++ +G +P ILGVREHVFTGSVSSLA FMS QETSFVTLGQRVL++PLKVRMHYGHPDV
Sbjct: 508 SQNHGKFRPSILGVREHVFTGSVSSLASFMSNQETSFVTLGQRVLSNPLKVRMHYGHPDV 687
Query: 93 FDRLWFLGRGGISKASRIIN 34
FDR++ + RGGISKASRIIN
Sbjct: 688 FDRIFHITRGGISKASRIIN 747
Score = 83.5 bits (176), Expect(4) = 2e-026
Identities = 37/49 (75%), Positives = 39/49 (79%)
Frame = +2 / -1
Query: 89 SKTSGWP*CILTLSGSARTRCPRVTKLVSWADMNQAREETEPVKTCSRT 235
S TSG P*CILT SG RTRCP VTKLVS D+N+AREET PVKTCS T
Sbjct: 692 SNTSG*P*CILTFSGLERTRCPNVTKLVS*FDINEAREETLPVKTCSLT 546
Score = 44.1 bits (90), Expect(4) = 2e-026
Identities = 23/32 (71%), Positives = 25/32 (78%)
Frame = +2 / -1
Query: 275 NSSSRFLIFRASSK*LSWFISIV*TASPLVKM 370
NSSSRFLI ASSK*LS FIS+V TA P V +
Sbjct: 506 NSSSRFLILSASSK*LS*FISMVCTAFPRVNI 411
Score = 44.1 bits (90), Expect(2) = 3e-079
Identities = 17/34 (50%), Positives = 22/34 (64%)
Frame = -3 / +1
Query: 624 CQVYXQQKAKNDPNAXXILELMKNYEALRVAYVD 523
CQ+Y QK + P A I LM+ EALR+AY+D
Sbjct: 151 CQIYGVQKGEGKPEAADIALLMQRNEALRIAYID 252
Score = 27.6 bits (54), Expect(4) = 2e-026
Identities = 15/34 (44%), Positives = 18/34 (52%)
Frame = +1 / -3
Query: 523 INISNT*CFIIFH*LQYXXSIGVILSFLLXIYLA 624
I++SN+ FI H Y S SFL IYLA
Sbjct: 252 IDVSNSESFISLHQKGYICSFWFPFSFLHSIYLA 151
Score = 21.7 bits (41), Expect(4) = 2e-026
Identities = 8/12 (66%), Positives = 11/12 (91%)
Frame = +2 / -1
Query: 32 MLIILDALLIPP 67
+L+I DALL+PP
Sbjct: 749 ILMIRDALLMPP 714
>gb|BQ902766.1|BQ902766 Ta03_01c07_R
Ta03_AAFC_ECORC_Fusarium_graminearum_inoculated_wheat_he
ads Triticum aestivum cDNA clone Ta03_01c07, mRNA
sequence
Length = 559
Score = 230 bits (496), Expect = 1e-059
Identities = 98/114 (85%), Positives = 106/114 (92%)
Frame = -3 / -2
Query: 345 QTIDMNQDNYFEEALKMRNLLEEFNRYYGIRKPKILGVREHVFTGSVSSLAWFMSAQETS 166
QTIDMNQDNYFEEALKMRNLLEEF++ +G KP ILGVREHVFTGSVSSLA FMS QETS
Sbjct: 558 QTIDMNQDNYFEEALKMRNLLEEFSQNHGKFKPSILGVREHVFTGSVSSLASFMSNQETS 379
Query: 165 FVTLGQRVLADPLKVRMHYGHPDVFDRLWFLGRGGISKASRIINISEDIFAGFN 4
FVTLGQRVL++PLKVRMHYGHPDVFDR++ + RGGISKASRIINISEDIFAGFN
Sbjct: 378 FVTLGQRVLSNPLKVRMHYGHPDVFDRIFHITRGGISKASRIINISEDIFAGFN 217
Score = 83.5 bits (176), Expect(3) = 4e-027
Identities = 37/49 (75%), Positives = 39/49 (79%)
Frame = +2 / +2
Query: 89 SKTSGWP*CILTLSGSARTRCPRVTKLVSWADMNQAREETEPVKTCSRT 235
S TSG P*CILT SG RTRCP VTKLVS D+N+AREET PVKTCS T
Sbjct: 302 SNTSG*P*CILTFSGLERTRCPNVTKLVS*FDINEAREETLPVKTCSLT 448
Score = 40.9 bits (83), Expect(3) = 4e-027
Identities = 20/23 (86%), Positives = 21/23 (91%)
Frame = +2 / +2
Query: 275 NSSSRFLIFRASSK*LSWFISIV 343
NSSSRFLIF ASSK*LS FIS+V
Sbjct: 488 NSSSRFLIFSASSK*LS*FISMV 556
Score = 35.4 bits (71), Expect(3) = 4e-027
Identities = 14/22 (63%), Positives = 19/22 (86%)
Frame = +2 / +2
Query: 2 QLKPAKMSSLMLIILDALLIPP 67
+L PA +SSL+L+I DALL+PP
Sbjct: 215 ELNPANISSLILMIRDALLMPP 280
>gb|BU099331.1|BU099331 WHE3306_C03_E06ZS Chinese Spring wheat drought stressed root cDNA
library Triticum aestivum cDNA clone WHE3306_C03_E06,
mRNA sequence
Length = 646
Score = 190 bits (410), Expect(2) = 9e-057
Identities = 80/105 (76%), Positives = 91/105 (86%)
Frame = -3 / +1
Query: 444 EIYRVKLPGELKIGEGKPENQNHALIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEFNRY 265
EIYR+KLPG IGEGKPENQNHA+IFTRG+A+QTIDMNQDNY EEA KMRN+L+EF R+
Sbjct: 331 EIYRIKLPGPPIIGEGKPENQNHAIIFTRGEALQTIDMNQDNYLEEAYKMRNVLQEFVRH 510
Query: 264 YGIRKPKILGVREHVFTGSVSSLAWFMSAQETSFVTLGQRVLADP 130
+ P ILG+REH+FTGSVSSLA FMS QETSFVT+GQR LADP
Sbjct: 511 PRDQTPTILGLREHIFTGSVSSLAGFMSYQETSFVTIGQRFLADP 645
Score = 50.6 bits (104), Expect(2) = 9e-057
Identities = 18/39 (46%), Positives = 29/39 (74%)
Frame = -3 / +1
Query: 627 ACQVYXQQKAKNDPNAXXILELMKNYEALRVAYVDERQI 511
+CQ + +QK+ D +A I++LM Y ALRVAY++E++I
Sbjct: 145 SCQKFGEQKSNGDAHAQDIIDLMARYPALRVAYIEEKEI 261
>gb|BE498502.1|BE498502 WHE0967_A12_A23ZS Wheat pre-anthesis spike cDNA library Triticum
aestivum cDNA clone WHE0967_A12_A23, mRNA sequence
Length = 559
Score = 167 bits (360), Expect(2) = 7e-048
Identities = 69/91 (75%), Positives = 78/91 (85%)
Frame = -3 / +2
Query: 453 REVEIYRVKLPGELKIGEGKPENQNHALIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEF 274
++ EIY +KLP K+GEGKPENQNHA+IFTRGDA+QTIDMNQDNY EEA+K+RN LEEF
Sbjct: 287 KDQEIYSIKLPXNPKLGEGKPENQNHAIIFTRGDAIQTIDMNQDNYLEEAMKVRNXLEEF 466
Query: 273 NRYYGIRKPKILGVREHVFTGSVSSLAWFMS 181
+GIR P ILGVREHVFTGSVSSLA FMS
Sbjct: 467 RGNHGIRYPTILGVREHVFTGSVSSLASFMS 559
Score = 43.7 bits (89), Expect(2) = 7e-048
Identities = 17/36 (47%), Positives = 24/36 (66%)
Frame = -3 / +2
Query: 627 ACQVYXQQKAKNDPNAXXILELMKNYEALRVAYVDE 520
+CQ+Y QQK + P A I L++ EALRVA++ E
Sbjct: 104 SCQIYGQQKQRKAPEAADIALLLQRNEALRVAFIHE 211
>gb|DQ086486.1| Triticum aestivum putative 1,3-beta-glucan synthase 10 (GSL10)
mRNA, partial cds
Length = 550
Score = 156 bits (335), Expect(2) = 2e-046
Identities = 65/88 (73%), Positives = 76/88 (86%)
Frame = -3 / +1
Query: 444 EIYRVKLPGELKIGEGKPENQNHALIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEFNRY 265
EIYR+KLPG IGEGKPENQ+HA+IFTRG+A+QTIDMNQDNY EEA KMRN+L+EF R+
Sbjct: 283 EIYRIKLPGPPIIGEGKPENQDHAIIFTRGEALQTIDMNQDNYLEEAYKMRNVLQEFVRH 462
Query: 264 YGIRKPKILGVREHVFTGSVSSLAWFMS 181
+ P ILG+REH+FTGSVSSLA FMS
Sbjct: 463 PRDQTPTILGLREHIFTGSVSSLAGFMS 546
Score = 50.1 bits (103), Expect(2) = 2e-046
Identities = 18/39 (46%), Positives = 29/39 (74%)
Frame = -3 / +1
Query: 627 ACQVYXQQKAKNDPNAXXILELMKNYEALRVAYVDERQI 511
+CQ + +QK+ D +A I++LM Y ALRVAY++E++I
Sbjct: 97 SCQKFGEQKSNGDVHAQDIIDLMARYPALRVAYIEEKEI 213
>gb|BQ743211.1|BQ743211 WHE4101_D09_G17ZS Wheat salt-stressed root cDNA library Triticum
aestivum cDNA clone WHE4101_D09_G17, mRNA sequence
Length = 705
Score = 113 bits (242), Expect(2) = 1e-033
Identities = 45/56 (80%), Positives = 51/56 (91%)
Frame = -3 / +3
Query: 444 EIYRVKLPGELKIGEGKPENQNHALIFTRGDAVQTIDMNQDNYFEEALKMRNLLEE 277
EIYR+KLPG IGEGKPENQNHA+IFTRG+A+QTIDMNQDNY EEA KMRN+L+E
Sbjct: 537 EIYRIKLPGPPIIGEGKPENQNHAIIFTRGEALQTIDMNQDNYLEEAYKMRNVLQE 704
Score = 50.1 bits (103), Expect(2) = 1e-033
Identities = 18/39 (46%), Positives = 29/39 (74%)
Frame = -3 / +3
Query: 627 ACQVYXQQKAKNDPNAXXILELMKNYEALRVAYVDERQI 511
+CQ + +QK+ D +A I++LM Y ALRVAY++E++I
Sbjct: 351 SCQKFGEQKSNGDVHAQDIIDLMARYPALRVAYIEEKEI 467
>gb|CF133118.1|CF133118 WHE4354_C05_F10ZT Wheat meiotic floret cDNA library Triticum
aestivum cDNA clone WHE4354_C05_F10, mRNA sequence
Length = 779
Score = 118 bits (252), Expect = 6e-026
Identities = 48/55 (87%), Positives = 53/55 (96%)
Frame = -3 / +1
Query: 168 SFVTLGQRVLADPLKVRMHYGHPDVFDRLWFLGRGGISKASRIINISEDIFAGFN 4
SFVTLGQRVL++PLKVRMHYGHPDVFDR++ + RGGISKASRIINISEDIFAGFN
Sbjct: 31 SFVTLGQRVLSNPLKVRMHYGHPDVFDRIFHITRGGISKASRIINISEDIFAGFN 195
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 553,520,629
Number of Sequences: 636343
Number of extensions: 7750301
Number of successful extensions: 69955
Number of sequences better than 1.0e-020: 9
length of database: 122,413,413
effective HSP length: 53
effective length of database: 88,687,234
effective search space used: 13923895738
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)