TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCG13d12.yg.2.1
(755 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA497978.1|CA497978 WHE3236_G04_N08ZT Wheat meiotic anth... 329 2e-089
gb|CA501322.1|CA501322 WHE4032_B11_D22ZT Wheat meiotic anth... 272 2e-074
gb|BQ902766.1|BQ902766 Ta03_01c07_R Ta03_AAFC_ECORC_Fusariu... 263 2e-069
gb|BU099331.1|BU099331 WHE3306_C03_E06ZS Chinese Spring whe... 193 4e-053
gb|DQ086486.1| Triticum aestivum putative 1,3-beta-glucan s... 160 4e-043
gb|BE498502.1|BE498502 WHE0967_A12_A23ZS Wheat pre-anthesis... 169 4e-041
gb|CF133118.1|CF133118 WHE4354_C05_F10ZT Wheat meiotic flor... 158 8e-038
gb|BQ743211.1|BQ743211 WHE4101_D09_G17ZS Wheat salt-stresse... 119 4e-031
>gb|CA497978.1|CA497978 WHE3236_G04_N08ZT Wheat meiotic anther cDNA library Triticum
aestivum cDNA clone WHE3236_G04_N08, mRNA sequence
Length = 633
Score = 329 bits (713), Expect = 2e-089
Identities = 129/165 (78%), Positives = 150/165 (90%)
Frame = -2 / +3
Query: 502 DQVIYKIKLPGNAILGEGKPENQNHAIIFTRGECLQTIDMNQEHYMEEALKMRNLLQEFE 323
D+ IY +KLPGN LGEGKPENQNHA+IFTRG +QTIDMNQ++Y EEALKMRNLL+EF
Sbjct: 138 DKEIYSVKLPGNPKLGEGKPENQNHAVIFTRGNAVQTIDMNQDNYFEEALKMRNLLEEFS 317
Query: 322 KKHGVRYPSMLGVREHIFTGSVSSLAWFMSNQETSFVTIGQRVLANPLRVRFHYGHPDIF 143
+ HG PS+LGVREH+FTGSVSSLA FMSNQETSFVT+GQRVL+NPL+VR HYGHPD+F
Sbjct: 318 QNHGKFRPSILGVREHVFTGSVSSLASFMSNQETSFVTLGQRVLSNPLKVRMHYGHPDVF 497
Query: 142 DRLFHITRGGVSKASKIINLSEDIFAGFNSTLREGNVTHHEYLPG 8
DR+FHITRGG+SKAS+IIN+SEDIFAGFNSTLR+GN+THHEY+ G
Sbjct: 498 DRIFHITRGGISKASRIINISEDIFAGFNSTLRQGNITHHEYIQG 632
Score = 151 bits (324), Expect(2) = 3e-043
Identities = 74/117 (63%), Positives = 82/117 (70%)
Frame = +3 / -3
Query: 9 PGRYSW*VTLPSRNVELNPANMSSLRLIILDALLTPPLVMWKRRSKISG*P**KRTLNGL 188
P YSW*V P R VELNPAN+SSL L+I DALL PPLV+ S SG*P* T +GL
Sbjct: 631 PWIYSW*VMFP*RRVELNPANISSLILMIRDALLMPPLVI*NILSNTSG*P*CILTFSGL 452
Query: 189 ASTRCPIVTKLVS*FDMNHARDDTLPVNMCSLTPSIDGYLTPCFFSNSCSRFLIFKA 359
TRCP VTKLVS*FD+N AR++TLPV CSLTP I+G P F NS SRFLI A
Sbjct: 451 ERTRCPNVTKLVS*FDINEAREETLPVKTCSLTPRIEGLNLP*F*ENSSSRFLILSA 281
Score = 145 bits (311), Expect = 6e-034
Identities = 65/142 (45%), Positives = 101/142 (71%)
Frame = +2 / -2
Query: 17 VLVVSNIAFTQCGIESCKYVLTEIDNFGCFTHTTPRNVEKAIEDIRMTIMKTNPQRIGKH 196
+L+VS + TQ ++ CKY+L +ID+ G T+ TPRN++ +I+ + +TIM ++ Q I K+
Sbjct: 623 ILMVSYVPLTQSRVKPCKYILADIDDTGRLTYATPRNMKYSIKHVWVTIMHSHFQWIRKN 444
Query: 197 SLSNCYETSFLI*HEPCKG*HTARKYVFSDSKHRWISHSMFLLKLLQQISHFQSLLHIVL 376
+LS C++TSFLI*H+ KG +T+ + +FS++K+ +M L + L+QISHF+ LL IV+
Sbjct: 443 TLS*CHKTSFLI*HKRSKGGNTSGEDMFSNTKN*RPELAMILREFLKQISHFECLLEIVI 264
Query: 377 LIHIYSLEAFASGEYYCMVLIF 442
LIHI L ++ EYY MVLIF
Sbjct: 263 LIHINGLYGISASEYYSMVLIF 198
Score = 100 bits (213), Expect(2) = 1e-025
Identities = 43/96 (44%), Positives = 62/96 (64%)
Frame = -1 / +2
Query: 443 RKSEPCNNIHQRRMPPNYRYESGALYGGGFENEKSAARV*EETWSEISIDAWSQRTHIYG 264
RKS+PC NIH R+ N+ YESG L+ GG +NEKSA + ++W + ++W RT ++
Sbjct: 197 RKSKPCCNIHSRKCRTNH*YESG*LFRGGTQNEKSA*GILSKSWQVQAFNSWC*RTCLHR 376
Query: 263 QCVIPCMVHVKSGN*FRNNWTTSACQSVEGSFSLWS 156
+C +PC V+VKSGN*F + T + S E +LWS
Sbjct: 377 KCFLPCFVYVKSGN*FCDIRTACSF*STESENALWS 484
Score = 45.1 bits (92), Expect(2) = 3e-043
Identities = 17/24 (70%), Positives = 18/24 (75%)
Frame = +3 / -3
Query: 408 PLVNIIAWF*FSGFPSPKMALPGS 479
P VNI AWF*FSG PSP LPG+
Sbjct: 232 PRVNITAWF*FSGLPSPSFGLPGN 161
Score = 36.8 bits (74), Expect(2) = 1e-025
Identities = 15/35 (42%), Positives = 23/35 (65%)
Frame = -1 / +2
Query: 128 HYEGWCE*SIQNYQSQ*GHICRIQFHIA*RQCYSP 24
+YEGW +* + +Q Q G+ICR+ + A R+ SP
Sbjct: 512 YYEGWHK*GVPYHQYQRGYICRV*LYSASREHNSP 616
>gb|CA501322.1|CA501322 WHE4032_B11_D22ZT Wheat meiotic anther cDNA library Triticum
aestivum cDNA clone WHE4032_B11_D22, mRNA sequence
Length = 749
Score = 272 bits (589), Expect(2) = 2e-074
Identities = 108/139 (77%), Positives = 125/139 (89%)
Frame = -2 / +1
Query: 502 DQVIYKIKLPGNAILGEGKPENQNHAIIFTRGECLQTIDMNQEHYMEEALKMRNLLQEFE 323
D+ IY +KLPGN LGEGKPENQNHA+IFTRG +QTIDMNQ++Y EEALKMRNLL+EF
Sbjct: 331 DKEIYSVKLPGNPKLGEGKPENQNHAVIFTRGNAVQTIDMNQDNYFEEALKMRNLLEEFS 510
Query: 322 KKHGVRYPSMLGVREHIFTGSVSSLAWFMSNQETSFVTIGQRVLANPLRVRFHYGHPDIF 143
+ HG PS+LGVREH+FTGSVSSLA FMSNQETSFVT+GQRVL+NPL+VR HYGHPD+F
Sbjct: 511 QNHGKFRPSILGVREHVFTGSVSSLASFMSNQETSFVTLGQRVLSNPLKVRMHYGHPDVF 690
Query: 142 DRLFHITRGGVSKASKIIN 86
DR+FHITRGG+SKAS+IIN
Sbjct: 691 DRIFHITRGGISKASRIIN 747
Score = 119 bits (255), Expect = 3e-026
Identities = 56/119 (47%), Positives = 85/119 (71%)
Frame = +2 / -3
Query: 86 IDNFGCFTHTTPRNVEKAIEDIRMTIMKTNPQRIGKHSLSNCYETSFLI*HEPCKG*HTA 265
ID+ G T+ TPRN++ +I+ + +TIM ++ Q I K++LS C++TSFLI*H+ KG +T+
Sbjct: 747 IDDTGRLTYATPRNMKYSIKHVWVTIMHSHFQWIRKNTLS*CHKTSFLI*HKRSKGGNTS 568
Query: 266 RKYVFSDSKHRWISHSMFLLKLLQQISHFQSLLHIVLLIHIYSLEAFASGEYYCMVLIF 442
+ +FS++K+ +M L + L+QISHF+ LL IV+LIHI L ++ EYY MVLIF
Sbjct: 567 GEDMFSNTKN*RPELAMILREFLKQISHFECLLEIVILIHINGLYGISASEYYSMVLIF 391
Score = 113 bits (242), Expect(2) = 5e-032
Identities = 56/91 (61%), Positives = 63/91 (69%)
Frame = +3 / -1
Query: 87 LIILDALLTPPLVMWKRRSKISG*P**KRTLNGLASTRCPIVTKLVS*FDMNHARDDTLP 266
L+I DALL PPLV+ S SG*P* T +GL TRCP VTKLVS*FD+N AR++TLP
Sbjct: 746 LMIRDALLMPPLVI*NILSNTSG*P*CILTFSGLERTRCPNVTKLVS*FDINEAREETLP 567
Query: 267 VNMCSLTPSIDGYLTPCFFSNSCSRFLIFKA 359
V CSLTP I+G P F NS SRFLI A
Sbjct: 566 VKTCSLTPRIEGLNLP*F*ENSSSRFLILSA 474
Score = 45.1 bits (92), Expect(2) = 5e-032
Identities = 17/24 (70%), Positives = 18/24 (75%)
Frame = +3 / -1
Query: 408 PLVNIIAWF*FSGFPSPKMALPGS 479
P VNI AWF*FSG PSP LPG+
Sbjct: 425 PRVNITAWF*FSGLPSPSFGLPGN 354
Score = 27.6 bits (54), Expect(2) = 2e-074
Identities = 15/46 (32%), Positives = 19/46 (41%)
Frame = -2 / +1
Query: 742 ADMXXXYXXXXXXYGIQXXXXEXXAHDILRLXTXYPSXXXAYIDEV 605
AD+ Y YG+Q + A DI L + AYID V
Sbjct: 121 ADLKFTYVVTCQIYGVQKGEGKPEAADIALLMQRNEALRIAYIDVV 258
>gb|BQ902766.1|BQ902766 Ta03_01c07_R
Ta03_AAFC_ECORC_Fusarium_graminearum_inoculated_wheat_he
ads Triticum aestivum cDNA clone Ta03_01c07, mRNA
sequence
Length = 559
Score = 263 bits (568), Expect = 2e-069
Identities = 103/128 (80%), Positives = 120/128 (93%)
Frame = -2 / -2
Query: 397 QTIDMNQEHYMEEALKMRNLLQEFEKKHGVRYPSMLGVREHIFTGSVSSLAWFMSNQETS 218
QTIDMNQ++Y EEALKMRNLL+EF + HG PS+LGVREH+FTGSVSSLA FMSNQETS
Sbjct: 558 QTIDMNQDNYFEEALKMRNLLEEFSQNHGKFKPSILGVREHVFTGSVSSLASFMSNQETS 379
Query: 217 FVTIGQRVLANPLRVRFHYGHPDIFDRLFHITRGGVSKASKIINLSEDIFAGFNSTLREG 38
FVT+GQRVL+NPL+VR HYGHPD+FDR+FHITRGG+SKAS+IIN+SEDIFAGFNSTLR+G
Sbjct: 378 FVTLGQRVLSNPLKVRMHYGHPDVFDRIFHITRGGISKASRIINISEDIFAGFNSTLRQG 199
Query: 37 NVTHHEYL 14
N+THHEY+
Sbjct: 198 NITHHEYI 175
Score = 153 bits (328), Expect = 3e-036
Identities = 74/114 (64%), Positives = 82/114 (71%)
Frame = +3 / +2
Query: 18 YSW*VTLPSRNVELNPANMSSLRLIILDALLTPPLVMWKRRSKISG*P**KRTLNGLAST 197
YSW*V P R VELNPAN+SSL L+I DALL PPLV+ S SG*P* T +GL T
Sbjct: 179 YSW*VMFP*RRVELNPANISSLILMIRDALLMPPLVI*NILSNTSG*P*CILTFSGLERT 358
Query: 198 RCPIVTKLVS*FDMNHARDDTLPVNMCSLTPSIDGYLTPCFFSNSCSRFLIFKA 359
RCP VTKLVS*FD+N AR++TLPV CSLTP I+G P F NS SRFLIF A
Sbjct: 359 RCPNVTKLVS*FDINEAREETLPVKTCSLTPRIEGLNLP*F*ENSSSRFLIFSA 520
Score = 126 bits (270), Expect = 3e-028
Identities = 58/127 (45%), Positives = 90/127 (70%)
Frame = +2 / +1
Query: 17 VLVVSNIAFTQCGIESCKYVLTEIDNFGCFTHTTPRNVEKAIEDIRMTIMKTNPQRIGKH 196
+L+VS + TQ ++ CKY+LT+ID+ G T TPRN++ +I+ + +TIM ++ Q I K+
Sbjct: 178 ILMVSYVPLTQSRVKPCKYILTDIDDTGRLTDATPRNMKYSIKHVWVTIMHSHFQWIRKN 357
Query: 197 SLSNCYETSFLI*HEPCKG*HTARKYVFSDSKHRWISHSMFLLKLLQQISHFQSLLHIVL 376
+LS C+ETSFLI*H+ +G +T+ + +FS++K + +M L + L+QISHF LL IV+
Sbjct: 358 ALS*CHETSFLI*HKRSEGGNTSSEDMFSNTKD*RLELAMILREFLKQISHF*CLLEIVI 537
Query: 377 LIHIYSL 397
LIHI L
Sbjct: 538 LIHINGL 558
>gb|BU099331.1|BU099331 WHE3306_C03_E06ZS Chinese Spring wheat drought stressed root cDNA
library Triticum aestivum cDNA clone WHE3306_C03_E06,
mRNA sequence
Length = 646
Score = 193 bits (415), Expect(2) = 4e-053
Identities = 82/109 (75%), Positives = 95/109 (87%)
Frame = -2 / +1
Query: 508 SLDQVIYKIKLPGNAILGEGKPENQNHAIIFTRGECLQTIDMNQEHYMEEALKMRNLLQE 329
+LDQ IY+IKLPG I+GEGKPENQNHAIIFTRGE LQTIDMNQ++Y+EEA KMRN+LQE
Sbjct: 319 NLDQEIYRIKLPGPPIIGEGKPENQNHAIIFTRGEALQTIDMNQDNYLEEAYKMRNVLQE 498
Query: 328 FEKKHGVRYPSMLGVREHIFTGSVSSLAWFMSNQETSFVTIGQRVLANP 182
F + + P++LG+REHIFTGSVSSLA FMS QETSFVTIGQR LA+P
Sbjct: 499 FVRHPRDQTPTILGLREHIFTGSVSSLAGFMSYQETSFVTIGQRFLADP 645
Score = 119 bits (254), Expect = 4e-026
Identities = 60/109 (55%), Positives = 70/109 (64%)
Frame = +3 / -3
Query: 183 GLASTRCPIVTKLVS*FDMNHARDDTLPVNMCSLTPSIDGYLTPCFFSNSCSRFLIFKAX 362
G A CPIVTKLVS +DMN A D+TLPV +CSL+P + G + +NS S FLI A
Sbjct: 644 GSARNLCPIVTKLVSWYDMNPASDETLPVKICSLSPRMVGVWSLGCLTNS*STFLIL*AS 465
Query: 363 XXXXXXXXXXVWRHSPLVNIIAWF*FSGFPSPKMALPGSFIL*MT*SRL 509
V R SP VNIIAWF*FSGFPSP M PGSFI *++ S+L
Sbjct: 464 SK*LS*FMSIVCRASPRVNIIAWF*FSGFPSPMMGGPGSFIR*ISWSKL 318
Score = 36.3 bits (73), Expect(2) = 4e-053
Identities = 16/45 (35%), Positives = 21/45 (46%)
Frame = -2 / +1
Query: 742 ADMXXXYXXXXXXYGIQXXXXEXXAHDILRLXTXYPSXXXAYIDE 608
ADM Y +G Q + A DI+ L YP+ AYI+E
Sbjct: 118 ADMKFSYVISCQKFGEQKSNGDAHAQDIIDLMARYPALRVAYIEE 252
>gb|DQ086486.1| Triticum aestivum putative 1,3-beta-glucan synthase 10 (GSL10)
mRNA, partial cds
Length = 550
Score = 160 bits (343), Expect(2) = 4e-043
Identities = 67/92 (72%), Positives = 80/92 (86%)
Frame = -2 / +1
Query: 508 SLDQVIYKIKLPGNAILGEGKPENQNHAIIFTRGECLQTIDMNQEHYMEEALKMRNLLQE 329
+LDQ IY+IKLPG I+GEGKPENQ+HAIIFTRGE LQTIDMNQ++Y+EEA KMRN+LQE
Sbjct: 271 NLDQEIYRIKLPGPPIIGEGKPENQDHAIIFTRGEALQTIDMNQDNYLEEAYKMRNVLQE 450
Query: 328 FEKKHGVRYPSMLGVREHIFTGSVSSLAWFMS 233
F + + P++LG+REHIFTGSVSSLA FMS
Sbjct: 451 FVRHPRDQTPTILGLREHIFTGSVSSLAGFMS 546
Score = 35.9 bits (72), Expect(2) = 4e-043
Identities = 16/45 (35%), Positives = 21/45 (46%)
Frame = -2 / +1
Query: 742 ADMXXXYXXXXXXYGIQXXXXEXXAHDILRLXTXYPSXXXAYIDE 608
ADM Y +G Q + A DI+ L YP+ AYI+E
Sbjct: 70 ADMKFSYVISCQKFGEQKSNGDVHAQDIIDLMARYPALRVAYIEE 204
>gb|BE498502.1|BE498502 WHE0967_A12_A23ZS Wheat pre-anthesis spike cDNA library Triticum
aestivum cDNA clone WHE0967_A12_A23, mRNA sequence
Length = 559
Score = 169 bits (363), Expect = 4e-041
Identities = 67/90 (74%), Positives = 79/90 (87%)
Frame = -2 / +2
Query: 502 DQVIYKIKLPGNAILGEGKPENQNHAIIFTRGECLQTIDMNQEHYMEEALKMRNLLQEFE 323
DQ IY IKLP N LGEGKPENQNHAIIFTRG+ +QTIDMNQ++Y+EEA+K+RN L+EF
Sbjct: 290 DQEIYSIKLPXNPKLGEGKPENQNHAIIFTRGDAIQTIDMNQDNYLEEAMKVRNXLEEFR 469
Query: 322 KKHGVRYPSMLGVREHIFTGSVSSLAWFMS 233
HG+RYP++LGVREH+FTGSVSSLA FMS
Sbjct: 470 GNHGIRYPTILGVREHVFTGSVSSLASFMS 559
>gb|CF133118.1|CF133118 WHE4354_C05_F10ZT Wheat meiotic floret cDNA library Triticum
aestivum cDNA clone WHE4354_C05_F10, mRNA sequence
Length = 779
Score = 158 bits (339), Expect = 8e-038
Identities = 58/73 (79%), Positives = 69/73 (94%)
Frame = -2 / +1
Query: 232 NQETSFVTIGQRVLANPLRVRFHYGHPDIFDRLFHITRGGVSKASKIINLSEDIFAGFNS 53
N SFVT+GQRVL+NPL+VR HYGHPD+FDR+FHITRGG+SKAS+IIN+SEDIFAGFNS
Sbjct: 19 NSRASFVTLGQRVLSNPLKVRMHYGHPDVFDRIFHITRGGISKASRIINISEDIFAGFNS 198
Query: 52 TLREGNVTHHEYL 14
TLR+GN+THHEY+
Sbjct: 199 TLRQGNITHHEYI 237
>gb|BQ743211.1|BQ743211 WHE4101_D09_G17ZS Wheat salt-stressed root cDNA library Triticum
aestivum cDNA clone WHE4101_D09_G17, mRNA sequence
Length = 705
Score = 119 bits (255), Expect(2) = 4e-031
Identities = 48/60 (80%), Positives = 55/60 (91%)
Frame = -2 / +3
Query: 508 SLDQVIYKIKLPGNAILGEGKPENQNHAIIFTRGECLQTIDMNQEHYMEEALKMRNLLQE 329
+LDQ IY+IKLPG I+GEGKPENQNHAIIFTRGE LQTIDMNQ++Y+EEA KMRN+LQE
Sbjct: 525 NLDQEIYRIKLPGPPIIGEGKPENQNHAIIFTRGEALQTIDMNQDNYLEEAYKMRNVLQE 704
Score = 35.9 bits (72), Expect(2) = 4e-031
Identities = 16/45 (35%), Positives = 21/45 (46%)
Frame = -2 / +3
Query: 742 ADMXXXYXXXXXXYGIQXXXXEXXAHDILRLXTXYPSXXXAYIDE 608
ADM Y +G Q + A DI+ L YP+ AYI+E
Sbjct: 324 ADMKFSYVISCQKFGEQKSNGDVHAQDIIDLMARYPALRVAYIEE 458
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 633,313,523
Number of Sequences: 636343
Number of extensions: 9106784
Number of successful extensions: 78566
Number of sequences better than 1.0e-020: 9
length of database: 122,413,413
effective HSP length: 53
effective length of database: 88,687,234
effective search space used: 17560072332
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)