TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCA10g02.yg.2.1
(277 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BJ244687.1|BJ244687 BJ244687 Y. Ogihara unpublished cDNA... 196 1e-049
gb|BJ248938.1|BJ248938 BJ248938 Y. Ogihara unpublished cDNA... 196 1e-049
gb|BE499840.1|BE499840 WHE0979_F04_K07ZS Wheat pre-anthesis... 120 1e-039
gb|AF347064.1| Triticum aestivum sucrose-phosphate synthase... 120 5e-039
gb|BJ317548.1|BJ317548 BJ317548 Y. Ogihara unpublished cDNA... 120 6e-034
gb|AF534907.1| Triticum aestivum sucrose-phosphate synthase... 104 1e-029
gb|CK210266.1|CK210266 FGAS022067 Triticum aestivum FGAS: L... 91 1e-028
gb|DR739628.1|DR739628 FGAS084845 Triticum aestivum FGAS: L... 91 1e-028
gb|BJ259967.1|BJ259967 BJ259967 Y. Ogihara unpublished cDNA... 107 4e-023
gb|CV766069.1|CV766069 FGAS060456 Triticum aestivum FGAS: L... 105 2e-022
gb|CA716620.1|CA716620 wdk3c.pk026.d4 wdk3c Triticum aestiv... 100 4e-022
>gb|BJ244687.1|BJ244687 BJ244687 Y. Ogihara unpublished cDNA library, Wh_f Triticum
aestivum cDNA clone whf16c08 5', mRNA sequence
Length = 651
Score = 196 bits (423), Expect = 1e-049
Identities = 78/91 (85%), Positives = 84/91 (92%)
Frame = -3 / +2
Query: 275 RVDLFTRQVSSPDVDWSYGEPTEMLCSGSNDGEGMGESAGAYIVRIPCGPRDKYLKKEAL 96
RVDLFTRQVSSPDVDWSYGEPTEML SGS+D EG GES GAYIVRIPCG +KY+KKE+L
Sbjct: 230 RVDLFTRQVSSPDVDWSYGEPTEMLTSGSHDAEGSGESGGAYIVRIPCGQSNKYIKKESL 409
Query: 95 WPYLQEFVDGALAHILNMSKALGEQVGNGRP 3
WPYLQEFVDGALAHILNMSK LGEQVG+G+P
Sbjct: 410 WPYLQEFVDGALAHILNMSKVLGEQVGHGKP 502
Score = 109 bits (232), Expect = 1e-023
Identities = 49/90 (54%), Positives = 65/90 (72%)
Frame = +3 / -3
Query: 3 WPPISNLFSQSLGHVKDMRKSSIDKLLEVRPQCFLLEVFIPWSTRYAHNVGSGTLTHPLS 182
W P++ LF+Q+L HV ++RK S++KLLEVRPQ LL+V + + R AHNV LT PL
Sbjct: 502 WLPMAYLFAQNL*HV*NVRKGSVNKLLEVRPQGLLLDVLVTLAARDAHNVCPAALTAPLC 323
Query: 183 IVGTGA*HLRRLTIAPVHVRR*HLTSEEVH 272
+VGTG *HL L +APVHV *HL S++V+
Sbjct: 322 VVGTGG*HLCWLPVAPVHVG**HLASKQVY 233
Score = 107 bits (229), Expect = 3e-023
Identities = 52/92 (56%), Positives = 62/92 (67%)
Frame = -2 / +1
Query: 276 QGGPLHSSSVIS*RGLELW*ADGDVMLRFQRWRGDG*ECRSLHCAHTVWTTG*IPQEGST 97
Q P++S SVI+ RGLEL A+ DV LRF R RG+ *E R +HCAH + + QEG
Sbjct: 229 QSRPVYSPSVITRRGLELRGANRDVNLRFPRRRGER*ERRGIHCAHPLRPE*QVHQEGVP 408
Query: 96 VALPPRVCRWSSCAYP*HVQGSGRTGWKWEAS 1
VALPPRVC S CA+ HV+G GRTG WEAS
Sbjct: 409 VALPPRVC*RSPCAHSKHVKGFGRTGRPWEAS 504
Score = 104 bits (222), Expect = 2e-022
Identities = 49/89 (55%), Positives = 53/89 (59%)
Frame = -1 / +3
Query: 268 TSSLVKCHLLTWTGAMVSRRRCYAPVPTMERGWVRVPEPTLCAYRVDHGINTSRRKHCGL 89
T L KCH TWTGA S++RC PVPT +RG VR TLCA +TSRR CGL
Sbjct: 237 TCLLAKCHHPTWTGATGSQQRC*PPVPTTQRGAVRAAGHTLCASLAARVTSTSRRSPCGL 416
Query: 88 TSKSLSMELLRISLTCPRLWENRLEMGGQ 2
TSKSL E LR TC R W NR MG Q
Sbjct: 417 TSKSLLTEPLRTF*TCQRFWANR*AMGSQ 503
Score = 93.2 bits (197), Expect(2) = 1e-028
Identities = 36/50 (72%), Positives = 39/50 (78%)
Frame = +1 / -1
Query: 1 TGLPFPTCSPRALDMLRICARAPSTNSWR*GHSASFLRYLSRGPHGMRTM 150
TG P+PTCSP+ DM R+CARAPSTNSWR*GH SFL YL P GMRTM
Sbjct: 504 TGFPWPTCSPKTFDMFRMCARAPSTNSWR*GHRDSFLMYLLLWPQGMRTM 355
Score = 50.6 bits (104), Expect(2) = 1e-028
Identities = 23/25 (92%), Positives = 23/25 (92%)
Frame = +1 / -1
Query: 202 NISVGSP*LQSTSGDDT*RVKRSTL 276
NISVGSP*LQSTSGDDT RV RSTL
Sbjct: 303 NISVGSP*LQSTSGDDTWRVNRSTL 229
>gb|BJ248938.1|BJ248938 BJ248938 Y. Ogihara unpublished cDNA library, Wh_f Triticum
aestivum cDNA clone whf8l03 5', mRNA sequence
Length = 653
Score = 196 bits (423), Expect = 1e-049
Identities = 78/91 (85%), Positives = 84/91 (92%)
Frame = -3 / +1
Query: 275 RVDLFTRQVSSPDVDWSYGEPTEMLCSGSNDGEGMGESAGAYIVRIPCGPRDKYLKKEAL 96
RVDLFTRQVSSPDVDWSYGEPTEML SGS+D EG GES GAYIVRIPCG +KY+KKE+L
Sbjct: 61 RVDLFTRQVSSPDVDWSYGEPTEMLTSGSHDAEGSGESGGAYIVRIPCGQSNKYIKKESL 240
Query: 95 WPYLQEFVDGALAHILNMSKALGEQVGNGRP 3
WPYLQEFVDGALAHILNMSK LGEQVG+G+P
Sbjct: 241 WPYLQEFVDGALAHILNMSKVLGEQVGHGKP 333
Score = 109 bits (232), Expect = 1e-023
Identities = 49/90 (54%), Positives = 65/90 (72%)
Frame = +3 / -3
Query: 3 WPPISNLFSQSLGHVKDMRKSSIDKLLEVRPQCFLLEVFIPWSTRYAHNVGSGTLTHPLS 182
W P++ LF+Q+L HV ++RK S++KLLEVRPQ LL+V + + R AHNV LT PL
Sbjct: 333 WLPMAYLFAQNL*HV*NVRKGSVNKLLEVRPQGLLLDVLVTLAARDAHNVCPAALTAPLC 154
Query: 183 IVGTGA*HLRRLTIAPVHVRR*HLTSEEVH 272
+VGTG *HL L +APVHV *HL S++V+
Sbjct: 153 VVGTGG*HLCWLPVAPVHVG**HLASKQVY 64
Score = 107 bits (229), Expect = 3e-023
Identities = 52/92 (56%), Positives = 62/92 (67%)
Frame = -2 / +3
Query: 276 QGGPLHSSSVIS*RGLELW*ADGDVMLRFQRWRGDG*ECRSLHCAHTVWTTG*IPQEGST 97
Q P++S SVI+ RGLEL A+ DV LRF R RG+ *E R +HCAH + + QEG
Sbjct: 60 QSRPVYSPSVITRRGLELRGANRDVNLRFPRRRGER*ERRGIHCAHPLRPE*QVHQEGVP 239
Query: 96 VALPPRVCRWSSCAYP*HVQGSGRTGWKWEAS 1
VALPPRVC S CA+ HV+G GRTG WEAS
Sbjct: 240 VALPPRVC*RSPCAHSKHVKGFGRTGRPWEAS 335
Score = 104 bits (222), Expect = 2e-022
Identities = 49/89 (55%), Positives = 53/89 (59%)
Frame = -1 / +2
Query: 268 TSSLVKCHLLTWTGAMVSRRRCYAPVPTMERGWVRVPEPTLCAYRVDHGINTSRRKHCGL 89
T L KCH TWTGA S++RC PVPT +RG VR TLCA +TSRR CGL
Sbjct: 68 TCLLAKCHHPTWTGATGSQQRC*PPVPTTQRGAVRAAGHTLCASLAARVTSTSRRSPCGL 247
Query: 88 TSKSLSMELLRISLTCPRLWENRLEMGGQ 2
TSKSL E LR TC R W NR MG Q
Sbjct: 248 TSKSLLTEPLRTF*TCQRFWANR*AMGSQ 334
Score = 93.2 bits (197), Expect(2) = 1e-028
Identities = 36/50 (72%), Positives = 39/50 (78%)
Frame = +1 / -1
Query: 1 TGLPFPTCSPRALDMLRICARAPSTNSWR*GHSASFLRYLSRGPHGMRTM 150
TG P+PTCSP+ DM R+CARAPSTNSWR*GH SFL YL P GMRTM
Sbjct: 335 TGFPWPTCSPKTFDMFRMCARAPSTNSWR*GHRDSFLMYLLLWPQGMRTM 186
Score = 50.6 bits (104), Expect(2) = 1e-028
Identities = 23/25 (92%), Positives = 23/25 (92%)
Frame = +1 / -1
Query: 202 NISVGSP*LQSTSGDDT*RVKRSTL 276
NISVGSP*LQSTSGDDT RV RSTL
Sbjct: 134 NISVGSP*LQSTSGDDTWRVNRSTL 60
>gb|BE499840.1|BE499840 WHE0979_F04_K07ZS Wheat pre-anthesis spike cDNA library Triticum
aestivum cDNA clone WHE0979_F04_K07, mRNA sequence
Length = 488
Score = 120 bits (256), Expect(2) = 1e-039
Identities = 46/65 (70%), Positives = 55/65 (84%)
Frame = -3 / +2
Query: 197 SGSNDGEGMGESAGAYIVRIPCGPRDKYLKKEALWPYLQEFVDGALAHILNMSKALGEQV 18
+ N G+ MGES+GAYIVRIP GPR+KY+ KE LWP++QEFVDGAL HI+ MSK LGEQV
Sbjct: 89 NSENLGDDMGESSGAYIVRIPFGPREKYIPKEQLWPHIQEFVDGALVHIMQMSKVLGEQV 268
Query: 17 GNGRP 3
GNG+P
Sbjct: 269 GNGQP 283
Score = 61.1 bits (127), Expect(2) = 1e-039
Identities = 23/34 (67%), Positives = 28/34 (82%)
Frame = -3 / +2
Query: 275 RVDLFTRQVSSPDVDWSYGEPTEMLCSGSNDGEG 174
RVDL TRQ+S+PDVDWSYGEPTEML +++ G
Sbjct: 5 RVDLLTRQISAPDVDWSYGEPTEMLSPRNSENLG 106
>gb|AF347064.1| Triticum aestivum sucrose-phosphate synthase 2 mRNA, partial cds
Length = 3420
Score = 120 bits (256), Expect(2) = 5e-039
Identities = 46/65 (70%), Positives = 55/65 (84%)
Frame = -3 / +2
Query: 197 SGSNDGEGMGESAGAYIVRIPCGPRDKYLKKEALWPYLQEFVDGALAHILNMSKALGEQV 18
+ N G+ MGES+GAYIVRIP GPR+KY+ KE LWP++QEFVDGAL HI+ MSK LGEQV
Sbjct: 557 NSENLGDDMGESSGAYIVRIPFGPREKYIPKEQLWPHIQEFVDGALVHIMQMSKVLGEQV 736
Query: 17 GNGRP 3
GNG+P
Sbjct: 737 GNGQP 751
Score = 61.1 bits (127), Expect(2) = 5e-039
Identities = 23/34 (67%), Positives = 28/34 (82%)
Frame = -3 / +2
Query: 275 RVDLFTRQVSSPDVDWSYGEPTEMLCSGSNDGEG 174
RVDL TRQ+S+PDVDWSYGEPTEML +++ G
Sbjct: 473 RVDLLTRQISAPDVDWSYGEPTEMLSPRNSENLG 574
>gb|BJ317548.1|BJ317548 BJ317548 Y. Ogihara unpublished cDNA library, Wh_yf Triticum
aestivum cDNA clone whyf28l19 5', mRNA sequence
Length = 684
Score = 120 bits (256), Expect(2) = 6e-034
Identities = 46/65 (70%), Positives = 55/65 (84%)
Frame = -3 / +1
Query: 197 SGSNDGEGMGESAGAYIVRIPCGPRDKYLKKEALWPYLQEFVDGALAHILNMSKALGEQV 18
+ N G+ MGES+GAYIVRIP GPR+KY+ KE LWP++QEFVDGAL HI+ MSK LGEQV
Sbjct: 58 NSENLGDDMGESSGAYIVRIPFGPREKYIPKEQLWPHIQEFVDGALVHIMQMSKVLGEQV 237
Query: 17 GNGRP 3
GNG+P
Sbjct: 238 GNGQP 252
Score = 42.8 bits (87), Expect(2) = 6e-034
Identities = 15/25 (60%), Positives = 20/25 (80%)
Frame = -3 / +1
Query: 248 SSPDVDWSYGEPTEMLCSGSNDGEG 174
++PDVDWSYGEPTEML +++ G
Sbjct: 1 AAPDVDWSYGEPTEMLSPRNSENLG 75
>gb|AF534907.1| Triticum aestivum sucrose-phosphate synthase 9 mRNA, complete cds
Length = 3339
Score = 104 bits (221), Expect(2) = 1e-029
Identities = 38/62 (61%), Positives = 49/62 (79%)
Frame = -3 / +2
Query: 188 NDGEGMGESAGAYIVRIPCGPRDKYLKKEALWPYLQEFVDGALAHILNMSKALGEQVGNG 9
N + GE++G YI+RIP GPRD YL KE LWP++QEFVDGAL+HI+ MSK +GE++G G
Sbjct: 806 NSKQEKGENSGGYIIRIPFGPRDMYLTKERLWPFIQEFVDGALSHIVRMSKTIGEEIGCG 985
Query: 8 RP 3
P
Sbjct: 986 HP 991
Score = 45.1 bits (92), Expect(2) = 1e-029
Identities = 18/27 (66%), Positives = 21/27 (77%)
Frame = -3 / +2
Query: 275 RVDLFTRQVSSPDVDWSYGEPTEMLCS 195
RVDL TRQ+ +P+ D SYGEP EML S
Sbjct: 713 RVDLLTRQILAPNFDRSYGEPAEMLVS 793
>gb|CK210266.1|CK210266 FGAS022067 Triticum aestivum FGAS: Library 5 GATE 7 Triticum
aestivum cDNA, mRNA sequence
Length = 1149
Score = 90.9 bits (192), Expect(2) = 1e-028
Identities = 32/48 (66%), Positives = 43/48 (89%)
Frame = -3 / +1
Query: 161 AGAYIVRIPCGPRDKYLKKEALWPYLQEFVDGALAHILNMSKALGEQV 18
AGAYIVR+PCGPRD+Y+ KE LWP++ EFVD AL+H+ N+++ALGEQ+
Sbjct: 502 AGAYIVRLPCGPRDQYIPKEELWPHIPEFVDRALSHVTNVARALGEQL 645
Score = 55.1 bits (114), Expect(2) = 1e-028
Identities = 22/32 (68%), Positives = 25/32 (78%)
Frame = -3 / +1
Query: 275 RVDLFTRQVSSPDVDWSYGEPTEMLCSGSNDG 180
RVDL TRQ+S PDVDW+YGEP EML S+ G
Sbjct: 373 RVDLLTRQISCPDVDWTYGEPLEMLERLSSGG 468
>gb|DR739628.1|DR739628 FGAS084845 Triticum aestivum FGAS: Library 6 CAP GATE 1 Triticum
aestivum cDNA, mRNA sequence
Length = 1101
Score = 90.9 bits (192), Expect(2) = 1e-028
Identities = 32/48 (66%), Positives = 43/48 (89%)
Frame = -3 / +1
Query: 161 AGAYIVRIPCGPRDKYLKKEALWPYLQEFVDGALAHILNMSKALGEQV 18
AGAYIVR+PCGPRD+Y+ KE LWP++ EFVD AL+H+ N+++ALGEQ+
Sbjct: 379 AGAYIVRLPCGPRDQYIPKEELWPHIPEFVDRALSHVTNVARALGEQL 522
Score = 55.1 bits (114), Expect(2) = 1e-028
Identities = 22/32 (68%), Positives = 25/32 (78%)
Frame = -3 / +1
Query: 275 RVDLFTRQVSSPDVDWSYGEPTEMLCSGSNDG 180
RVDL TRQ+S PDVDW+YGEP EML S+ G
Sbjct: 250 RVDLLTRQISCPDVDWTYGEPLEMLERLSSGG 345
>gb|BJ259967.1|BJ259967 BJ259967 Y. Ogihara unpublished cDNA library, Wh_h Triticum
aestivum cDNA clone whh22p10 5', mRNA sequence
Length = 552
Score = 107 bits (229), Expect = 4e-023
Identities = 43/48 (89%), Positives = 44/48 (91%)
Frame = -3 / +3
Query: 275 RVDLFTRQVSSPDVDWSYGEPTEMLCSGSNDGEGMGESAGAYIVRIPC 132
RVDLFTRQVSSPDVDWSYGEPTEML SGS+D EG GES GAYIVRIPC
Sbjct: 408 RVDLFTRQVSSPDVDWSYGEPTEMLTSGSHDAEGSGESGGAYIVRIPC 551
>gb|CV766069.1|CV766069 FGAS060456 Triticum aestivum FGAS: Library 2 Gate 3 Triticum
aestivum cDNA, mRNA sequence
Length = 837
Score = 105 bits (224), Expect = 2e-022
Identities = 39/56 (69%), Positives = 49/56 (87%)
Frame = -3 / +1
Query: 170 GESAGAYIVRIPCGPRDKYLKKEALWPYLQEFVDGALAHILNMSKALGEQVGNGRP 3
GE++GAYI RIP GP+DKYL KE LWPY+QEFVDGAL+HI++MSK +GE++G G P
Sbjct: 127 GENSGAYITRIPFGPKDKYLAKEHLWPYVQEFVDGALSHIVHMSKIIGEEIGCGHP 294
>gb|CA716620.1|CA716620 wdk3c.pk026.d4 wdk3c Triticum aestivum cDNA clone wdk3c.pk026.d4 5'
end, mRNA sequence
Length = 397
Score = 100 bits (212), Expect(2) = 4e-022
Identities = 37/62 (59%), Positives = 47/62 (75%)
Frame = -3 / +1
Query: 188 NDGEGMGESAGAYIVRIPCGPRDKYLKKEALWPYLQEFVDGALAHILNMSKALGEQVGNG 9
N + GE++G YI+RIP GPRD YL KE LWP++QEFVD AL+HI+ MSK +GE +G G
Sbjct: 70 NSKQEKGENSGGYIIRIPFGPRDMYLTKERLWPFIQEFVDAALSHIVRMSKTIGEXIGCG 249
Query: 8 RP 3
P
Sbjct: 250 HP 255
Score = 22.1 bits (42), Expect(2) = 4e-022
Identities = 8/10 (80%), Positives = 8/10 (80%)
Frame = -3 / +1
Query: 224 YGEPTEMLCS 195
YGEP EML S
Sbjct: 28 YGEPAEMLVS 57
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 344,699,595
Number of Sequences: 636343
Number of extensions: 5640343
Number of successful extensions: 532403
Number of sequences better than 1.0e-020: 15
length of database: 122,413,413
effective HSP length: 49
effective length of database: 91,232,606
effective search space used: 3831769452
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)