TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBN10e11.xg.2.4
(664 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|U60754.1|TAU60754 Triticum aestivum delta-24-sterol meth... 94 5e-036
gb|CV765668.1|CV765668 FGAS060055 Triticum aestivum FGAS: L... 94 5e-036
gb|BE585605.1|BE585605 EST#5T7_B05_b5_041 KSU wheat Fusariu... 94 5e-036
gb|BJ271067.1|BJ271067 BJ271067 Y. Ogihara unpublished cDNA... 94 5e-036
gb|BJ225577.1|BJ225577 BJ225577 Y. Ogihara unpublished cDNA... 89 1e-033
gb|U60755.1|TAU60755 Triticum aestivum delta-24-sterol meth... 97 2e-033
gb|BE493112.1|BE493112 WHE0567_C02_E03ZE Triticum monococcu... 94 2e-032
gb|BG313627.1|BG313627 WHE2056_E07_I14ZS Wheat salt-stresse... 94 2e-032
gb|CN008962.1|CN008962 WHE2647_B12_D23ZE Wheat Fusarium gra... 65 6e-021
>gb|U60754.1|TAU60754 Triticum aestivum delta-24-sterol methyltransferase (TA-MT) mRNA,
complete cds
Length = 1392
Score = 94.1 bits (199), Expect(3) = 5e-036
Identities = 36/51 (70%), Positives = 41/51 (80%)
Frame = -2 / +2
Query: 153 GVYSEVYRVLKPGQYFALDEWCLTDLFDPNNAKHLTTKAEIELGDGLPDIR 1
G Y E+YRVLKPGQ FA+ EWC+TD +DPNNA H K EIELG+GLPDIR
Sbjct: 638 GCYKEIYRVLKPGQCFAVYEWCITDHYDPNNATHKRIKDEIELGNGLPDIR 790
Score = 66.1 bits (138), Expect(3) = 5e-036
Identities = 25/41 (60%), Positives = 30/41 (73%)
Frame = -2 / +2
Query: 366 SAGLSEQCCSLKGDFMNMPIADNTFDAAYAIQATCYAPEAV 244
S GL C +K DFM MP +DNTFDA YAI+ATC+AP+ V
Sbjct: 515 SVGLGATCDFVKADFMKMPFSDNTFDAVYAIEATCHAPDPV 637
Score = 29.9 bits (59), Expect(3) = 5e-036
Identities = 11/19 (57%), Positives = 13/19 (68%)
Frame = -2 / +2
Query: 513 SSTXXXGLXXNDYHVSRGK 457
SST GL NDY ++RGK
Sbjct: 446 SSTSVTGLNNNDYQITRGK 502
>gb|CV765668.1|CV765668 FGAS060055 Triticum aestivum FGAS: Library 2 Gate 3 Triticum
aestivum cDNA, mRNA sequence
Length = 816
Score = 94.1 bits (199), Expect(3) = 5e-036
Identities = 36/51 (70%), Positives = 41/51 (80%)
Frame = -2 / +2
Query: 153 GVYSEVYRVLKPGQYFALDEWCLTDLFDPNNAKHLTTKAEIELGDGLPDIR 1
G Y E+YRVLKPGQ FA+ EWC+TD +DPNNA H K EIELG+GLPDIR
Sbjct: 551 GCYKEIYRVLKPGQCFAVYEWCITDHYDPNNATHKRIKDEIELGNGLPDIR 703
Score = 66.1 bits (138), Expect(3) = 5e-036
Identities = 25/41 (60%), Positives = 30/41 (73%)
Frame = -2 / +2
Query: 366 SAGLSEQCCSLKGDFMNMPIADNTFDAAYAIQATCYAPEAV 244
S GL C +K DFM MP +DNTFDA YAI+ATC+AP+ V
Sbjct: 428 SVGLGATCDFVKADFMKMPFSDNTFDAVYAIEATCHAPDPV 550
Score = 29.9 bits (59), Expect(3) = 5e-036
Identities = 11/19 (57%), Positives = 13/19 (68%)
Frame = -2 / +2
Query: 513 SSTXXXGLXXNDYHVSRGK 457
SST GL NDY ++RGK
Sbjct: 359 SSTSVTGLNNNDYQITRGK 415
>gb|BE585605.1|BE585605 EST#5T7_B05_b5_041 KSU wheat Fusarium graminearum infected spike
cDNA library Triticum aestivum cDNA clone
EST#5T7_B05_b5_041, mRNA sequence
Length = 712
Score = 94.1 bits (199), Expect(3) = 5e-036
Identities = 36/51 (70%), Positives = 41/51 (80%)
Frame = -2 / +1
Query: 153 GVYSEVYRVLKPGQYFALDEWCLTDLFDPNNAKHLTTKAEIELGDGLPDIR 1
G Y E+YRVLKPGQ FA+ EWC+TD +DPNNA H K EIELG+GLPDIR
Sbjct: 256 GCYKEIYRVLKPGQCFAVYEWCITDHYDPNNATHKRIKDEIELGNGLPDIR 408
Score = 66.1 bits (138), Expect(3) = 5e-036
Identities = 25/41 (60%), Positives = 30/41 (73%)
Frame = -2 / +1
Query: 366 SAGLSEQCCSLKGDFMNMPIADNTFDAAYAIQATCYAPEAV 244
S GL C +K DFM MP +DNTFDA YAI+ATC+AP+ V
Sbjct: 133 SVGLGATCDFVKADFMKMPFSDNTFDAVYAIEATCHAPDPV 255
Score = 29.9 bits (59), Expect(3) = 5e-036
Identities = 11/19 (57%), Positives = 13/19 (68%)
Frame = -2 / +1
Query: 513 SSTXXXGLXXNDYHVSRGK 457
SST GL NDY ++RGK
Sbjct: 64 SSTSVTGLNNNDYQITRGK 120
>gb|BJ271067.1|BJ271067 BJ271067 Y. Ogihara unpublished cDNA library, Wh_oh Triticum
aestivum cDNA clone whoh23p14 5', mRNA sequence
Length = 650
Score = 94.1 bits (199), Expect(3) = 5e-036
Identities = 36/51 (70%), Positives = 41/51 (80%)
Frame = -2 / +1
Query: 153 GVYSEVYRVLKPGQYFALDEWCLTDLFDPNNAKHLTTKAEIELGDGLPDIR 1
G Y E+YRVLKPGQ FA+ EWC+TD +DPNNA H K EIELG+GLPDIR
Sbjct: 427 GCYKEIYRVLKPGQCFAVYEWCITDHYDPNNATHKRIKDEIELGNGLPDIR 579
Score = 66.1 bits (138), Expect(3) = 5e-036
Identities = 25/41 (60%), Positives = 30/41 (73%)
Frame = -2 / +1
Query: 366 SAGLSEQCCSLKGDFMNMPIADNTFDAAYAIQATCYAPEAV 244
S GL C +K DFM MP +DNTFDA YAI+ATC+AP+ V
Sbjct: 304 SVGLGATCDFVKADFMKMPFSDNTFDAVYAIEATCHAPDPV 426
Score = 29.9 bits (59), Expect(3) = 5e-036
Identities = 11/19 (57%), Positives = 13/19 (68%)
Frame = -2 / +1
Query: 513 SSTXXXGLXXNDYHVSRGK 457
SST GL NDY ++RGK
Sbjct: 235 SSTSVTGLNNNDYQITRGK 291
>gb|BJ225577.1|BJ225577 BJ225577 Y. Ogihara unpublished cDNA library, Wh_dL Triticum
aestivum cDNA clone whdl22m20 5', mRNA sequence
Length = 488
Score = 89.0 bits (188), Expect(3) = 1e-033
Identities = 34/50 (68%), Positives = 39/50 (78%)
Frame = -2 / +3
Query: 153 GVYSEVYRVLKPGQYFALDEWCLTDLFDPNNAKHLTTKAEIELGDGLPDI 4
G Y E+YRVLKPGQ FA+ EWC+TD +DPNNA H K EI LG+GLPDI
Sbjct: 330 GCYKEIYRVLKPGQCFAVYEWCITDHYDPNNATHKRIKDEIXLGNGLPDI 479
Score = 66.1 bits (138), Expect(3) = 1e-033
Identities = 25/41 (60%), Positives = 30/41 (73%)
Frame = -2 / +3
Query: 366 SAGLSEQCCSLKGDFMNMPIADNTFDAAYAIQATCYAPEAV 244
S GL C +K DFM MP +DNTFDA YAI+ATC+AP+ V
Sbjct: 207 SVGLGATCDFVKADFMKMPFSDNTFDAVYAIEATCHAPDPV 329
Score = 26.7 bits (52), Expect(3) = 1e-033
Identities = 10/19 (52%), Positives = 12/19 (63%)
Frame = -2 / +3
Query: 513 SSTXXXGLXXNDYHVSRGK 457
SST GL N Y ++RGK
Sbjct: 138 SSTSVTGLNNNXYQITRGK 194
>gb|U60755.1|TAU60755 Triticum aestivum delta-24-sterol methyltransferase (TA-MT) gene,
complete cds
Length = 4749
Score = 96.8 bits (205), Expect(3) = 2e-033
Identities = 37/56 (66%), Positives = 45/56 (80%)
Frame = -2 / +2
Query: 168 WLSQQGVYSEVYRVLKPGQYFALDEWCLTDLFDPNNAKHLTTKAEIELGDGLPDIR 1
+++Q G Y E+YRVLKPGQ FA+ EWC+TD +DPNNA H K EIELG+GLPDIR
Sbjct: 3029 FVAQVGCYKEIYRVLKPGQCFAVYEWCITDHYDPNNATHKRIKDEIELGNGLPDIR 3196
Score = 61.1 bits (127), Expect(3) = 2e-033
Identities = 21/34 (61%), Positives = 27/34 (79%)
Frame = -2 / +2
Query: 345 CCSLKGDFMNMPIADNTFDAAYAIQATCYAPEAV 244
C ++ DFM MP +DNTFDA YAI+ATC+AP+ V
Sbjct: 2843 CSEIQADFMKMPFSDNTFDAVYAIEATCHAPDPV 2944
Score = 31.8 bits (63), Expect(3) = 2e-033
Identities = 12/20 (60%), Positives = 14/20 (70%)
Frame = -2 / +1
Query: 513 SSTXXXGLXXNDYHVSRGKV 454
SST GL NDY ++RGKV
Sbjct: 2245 SSTSVTGLNNNDYQITRGKV 2304
>gb|BE493112.1|BE493112 WHE0567_C02_E03ZE Triticum monococcum vegetative apex cDNA library
Triticum monococcum cDNA clone WHE0567_C02_E03, mRNA
sequence
Length = 446
Score = 94.1 bits (199), Expect(2) = 2e-032
Identities = 36/51 (70%), Positives = 41/51 (80%)
Frame = -2 / +2
Query: 153 GVYSEVYRVLKPGQYFALDEWCLTDLFDPNNAKHLTTKAEIELGDGLPDIR 1
G Y E+YRVLKPGQ FA+ EWC+TD +DPNNA H K EIELG+GLPDIR
Sbjct: 170 GCYKEIYRVLKPGQCFAVYEWCITDHYDPNNATHKRIKDEIELGNGLPDIR 322
Score = 66.1 bits (138), Expect(2) = 2e-032
Identities = 25/41 (60%), Positives = 30/41 (73%)
Frame = -2 / +2
Query: 366 SAGLSEQCCSLKGDFMNMPIADNTFDAAYAIQATCYAPEAV 244
S GL C +K DFM MP +DNTFDA YAI+ATC+AP+ V
Sbjct: 47 SVGLGATCDFVKADFMKMPFSDNTFDAVYAIEATCHAPDPV 169
>gb|BG313627.1|BG313627 WHE2056_E07_I14ZS Wheat salt-stressed sheath cDNA library Triticum
aestivum cDNA clone WHE2056_E07_I14, mRNA sequence
Length = 403
Score = 94.1 bits (199), Expect(2) = 2e-032
Identities = 36/51 (70%), Positives = 41/51 (80%)
Frame = -2 / +3
Query: 153 GVYSEVYRVLKPGQYFALDEWCLTDLFDPNNAKHLTTKAEIELGDGLPDIR 1
G Y E+YRVLKPGQ FA+ EWC+TD +DPNNA H K EIELG+GLPDIR
Sbjct: 126 GCYKEIYRVLKPGQCFAVYEWCITDHYDPNNATHKRIKDEIELGNGLPDIR 278
Score = 66.1 bits (138), Expect(2) = 2e-032
Identities = 25/41 (60%), Positives = 30/41 (73%)
Frame = -2 / +3
Query: 366 SAGLSEQCCSLKGDFMNMPIADNTFDAAYAIQATCYAPEAV 244
S GL C +K DFM MP +DNTFDA YAI+ATC+AP+ V
Sbjct: 3 SVGLGATCDFVKADFMKMPFSDNTFDAVYAIEATCHAPDPV 125
>gb|CN008962.1|CN008962 WHE2647_B12_D23ZE Wheat Fusarium graminearum infected spike cDNA
library Triticum aestivum cDNA clone WHE2647_B12_D23,
mRNA sequence
Length = 587
Score = 64.8 bits (135), Expect(2) = 6e-021
Identities = 23/51 (45%), Positives = 36/51 (70%)
Frame = -2 / +2
Query: 156 QGVYSEVYRVLKPGQYFALDEWCLTDLFDPNNAKHLTTKAEIELGDGLPDI 4
+G+YSE++RVLKPG F + EW +TD +D +N +H + IE GDG+ ++
Sbjct: 329 KGIYSEIFRVLKPGGVFGVYEWLMTDEYDNDNLRHREIRLGIEQGDGISNM 481
Score = 56.5 bits (117), Expect(2) = 6e-021
Identities = 23/41 (56%), Positives = 28/41 (68%)
Frame = -2 / +2
Query: 375 HIFSAGLSEQCCSLKGDFMNMPIADNTFDAAYAIQATCYAP 253
+ F GLS Q +KGDFM M DN+FDA YAI+AT +AP
Sbjct: 200 YAFKEGLSNQLEFVKGDFMQMSFPDNSFDAVYAIEATVHAP 322
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 504,950,589
Number of Sequences: 636343
Number of extensions: 7092293
Number of successful extensions: 63346
Number of sequences better than 1.0e-020: 10
length of database: 122,413,413
effective HSP length: 53
effective length of database: 88,687,234
effective search space used: 14810768078
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)