TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAZ2b01.yg.2.1
(541 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CV767238.1|CV767238 FGAS061627 Triticum aestivum FGAS: L... 190 1e-047
gb|BJ269645.1|BJ269645 BJ269645 Y. Ogihara unpublished cDNA... 148 3e-040
gb|BE517739.1|BE517739 WHE0802_D03_G06ZS Wheat vernalized c... 148 6e-035
gb|CD911643.1|CD911643 G550.111L22F010521 G550 Triticum aes... 146 1e-034
gb|BU100218.1|BU100218 WHE3316_F04_L08ZS Chinese Spring whe... 139 2e-032
gb|CK211085.1|CK211085 FGAS022913 Triticum aestivum FGAS: L... 124 9e-028
gb|CA623472.1|CA623472 wl1n.pk0105.c2 wl1n Triticum aestivu... 114 9e-025
gb|CA621622.1|CA621622 wl1n.pk0075.g9 wl1n Triticum aestivu... 100 5e-021
>gb|CV767238.1|CV767238 FGAS061627 Triticum aestivum FGAS: Library 2 Gate 3 Triticum
aestivum cDNA, mRNA sequence
Length = 816
Score = 190 bits (409), Expect = 1e-047
Identities = 79/119 (66%), Positives = 100/119 (84%)
Frame = +3 / +3
Query: 12 LXNDVLDRAKIEAGKLELEAVPFDLRSLMDDVISLFSSKSREKCIELAVFVCDNVPKDVL 191
L N+VLDRAKIEAGKLELE+VPFDLRS++D+V+SLFSSKSREK IELAV+V + VP+ +L
Sbjct: 219 LINEVLDRAKIEAGKLELESVPFDLRSILDEVVSLFSSKSREKGIELAVYVSERVPEILL 398
Query: 192 GDPWRFRQILTNLVGNAVKFTERGHVFVRVCLADNSNMEAGQVLNGAMNGKDGRVDSTT 368
GDP RFRQI+TNLVGN++KFTERGH+FV+V LAD+SN+ + NG +G +D T+
Sbjct: 399 GDPGRFRQIITNLVGNSIKFTERGHIFVQVHLADHSNLATEPKVESVANGINGHIDETS 575
Score = 121 bits (259), Expect = 6e-027
Identities = 64/116 (55%), Positives = 70/116 (60%)
Frame = -1 / -3
Query: 367 VVESTLPSFPFMAPFRT*PASIFELSAKQTRTNT*PRSVNLTALPTKFVSICRNLQGSPR 188
+V S P PF + FE SA+ T T P SVNL PTKFV I RNL GSP
Sbjct: 574 LVSSICPLMPFATDSTFGSVARFEWSAR*TCTKMCPLSVNLIEFPTKFVII*RNLPGSPS 395
Query: 187 TSFGTLSHTNTASSMHFSRDFEENKEITSSMRERRSKGTASSSNFPASIFARSRTS 20
GTLS T TASS+ FS DFEEN+E TSS + RS GT SSSN PASI A S TS
Sbjct: 394 RISGTLSETYTASSIPFSLDFEENRETTSSRMDLRSNGTDSSSNLPASILALSSTS 227
>gb|BJ269645.1|BJ269645 BJ269645 Y. Ogihara unpublished cDNA library, Wh_oh Triticum
aestivum cDNA clone whoh22o12 5', mRNA sequence
Length = 664
Score = 148 bits (317), Expect(2) = 3e-040
Identities = 58/90 (64%), Positives = 78/90 (86%)
Frame = +3 / +2
Query: 12 LXNDVLDRAKIEAGKLELEAVPFDLRSLMDDVISLFSSKSREKCIELAVFVCDNVPKDVL 191
L N+VLD+AKIE+GKLELEAVPFDLR + DD++SLF K++EK +ELAV+V D VP+ ++
Sbjct: 35 LINEVLDQAKIESGKLELEAVPFDLRLVCDDILSLFCGKAQEKGLELAVYVSDQVPQTLI 214
Query: 192 GDPWRFRQILTNLVGNAVKFTERGHVFVRV 281
GDP R RQI+TNL+GN++KFTERGH+++ V
Sbjct: 215 GDPGRMRQIITNLMGNSIKFTERGHIYLTV 304
Score = 37.7 bits (76), Expect(2) = 3e-040
Identities = 13/25 (52%), Positives = 18/25 (72%)
Frame = +3 / +2
Query: 366 TNGAFNTLSGFEAAXXRNSWQYFXM 440
TN +NTLSG++ A R+SWQ F +
Sbjct: 359 TNNTYNTLSGYKVANRRHSWQSFQL 433
>gb|BE517739.1|BE517739 WHE0802_D03_G06ZS Wheat vernalized crown cDNA library Triticum
aestivum cDNA clone WHE0802_D03_G06, mRNA sequence
Length = 562
Score = 148 bits (317), Expect = 6e-035
Identities = 58/90 (64%), Positives = 78/90 (86%)
Frame = +3 / +3
Query: 12 LXNDVLDRAKIEAGKLELEAVPFDLRSLMDDVISLFSSKSREKCIELAVFVCDNVPKDVL 191
L N+VLD+AKIE+GKLELEAVPFDLR + DD++SLF K++EK +ELAV+V D VP+ ++
Sbjct: 207 LINEVLDQAKIESGKLELEAVPFDLRLVCDDILSLFCGKAQEKGLELAVYVSDQVPQTLI 386
Query: 192 GDPWRFRQILTNLVGNAVKFTERGHVFVRV 281
GDP R RQI+TNL+GN++KFTERGH+++ V
Sbjct: 387 GDPGRMRQIITNLMGNSIKFTERGHIYLTV 476
>gb|CD911643.1|CD911643 G550.111L22F010521 G550 Triticum aestivum cDNA clone G550111L22,
mRNA sequence
Length = 582
Score = 146 bits (314), Expect = 1e-034
Identities = 58/90 (64%), Positives = 77/90 (85%)
Frame = +3 / +3
Query: 12 LXNDVLDRAKIEAGKLELEAVPFDLRSLMDDVISLFSSKSREKCIELAVFVCDNVPKDVL 191
L N+VLD+AKIEAGKLELEAVP DLR + DD++SLF K++EK +ELAV+V D VP+ ++
Sbjct: 303 LINEVLDQAKIEAGKLELEAVPLDLRLVCDDILSLFCGKAQEKGLELAVYVSDQVPQTLM 482
Query: 192 GDPWRFRQILTNLVGNAVKFTERGHVFVRV 281
GDP R RQI+TNL+GN++KFTERGH+++ V
Sbjct: 483 GDPGRMRQIITNLMGNSIKFTERGHIYLTV 572
>gb|BU100218.1|BU100218 WHE3316_F04_L08ZS Chinese Spring wheat drought stressed root cDNA
library Triticum aestivum cDNA clone WHE3316_F04_L08,
mRNA sequence
Length = 686
Score = 139 bits (299), Expect = 2e-032
Identities = 59/77 (76%), Positives = 71/77 (92%)
Frame = +3 / +2
Query: 12 LXNDVLDRAKIEAGKLELEAVPFDLRSLMDDVISLFSSKSREKCIELAVFVCDNVPKDVL 191
L N+VLDRAKIEAGKLELE+VPFDLRS++D+V+SLFSSKSREK IELAV+V + VP+ +L
Sbjct: 455 LINEVLDRAKIEAGKLELESVPFDLRSILDEVVSLFSSKSREKGIELAVYVSERVPEILL 634
Query: 192 GDPWRFRQILTNLVGNA 242
GDP RFRQI+TNLVGN+
Sbjct: 635 GDPGRFRQIITNLVGNS 685
>gb|CK211085.1|CK211085 FGAS022913 Triticum aestivum FGAS: Library 5 GATE 7 Triticum
aestivum cDNA, mRNA sequence
Length = 1144
Score = 124 bits (265), Expect = 9e-028
Identities = 51/85 (60%), Positives = 67/85 (78%)
Frame = +3 / +1
Query: 114 LFSSKSREKCIELAVFVCDNVPKDVLGDPWRFRQILTNLVGNAVKFTERGHVFVRVCLAD 293
LFSSKSREK I+LAV+V + VP+ +LGDP RFRQI+TNLVGN++KFTERGH+FV+V LAD
Sbjct: 55 LFSSKSREKGIDLAVYVSERVPEILLGDPGRFRQIITNLVGNSIKFTERGHIFVQVHLAD 234
Query: 294 NSNMEAGQVLNGAMNGKDGRVDSTT 368
+SN+ + NG +G +D T+
Sbjct: 235 HSNLATEPKVESVANGINGHIDETS 309
>gb|CA623472.1|CA623472 wl1n.pk0105.c2 wl1n Triticum aestivum cDNA clone wl1n.pk0105.c2 5'
end, mRNA sequence
Length = 650
Score = 114 bits (243), Expect = 9e-025
Identities = 51/76 (67%), Positives = 53/76 (69%)
Frame = +2 / +2
Query: 314 SGPKWSHEWERW*S*FNN*WCLQYSEWV*SSXXTK*LAIFXDAPL**GVPFG*SXXXXF* 493
SGP WSHEW+RW*S* NN*WCLQY EWV*SS TK*LAIF APL *GV FG *
Sbjct: 2 SGPTWSHEWQRW*S*VNN*WCLQYFEWV*SSRQTK*LAIF*TAPLR*GVTFGMILRAKI* 181
Query: 494 SXXFXXCHTXDKYXXH 541
S F CHT +KY H
Sbjct: 182 SK*FKSCHTSNKY*GH 229
Score = 113 bits (241), Expect = 2e-024
Identities = 50/74 (67%), Positives = 54/74 (72%)
Frame = -2 / -3
Query: 534 QYLSXV*XXXNXFDQNXXXQDHPKGTPYQRGASXNIANYFVXXLLQTHSEY*RHH*LLNQ 355
QYL V* N FD + PK TPY+RGA NIANYFV LLQTHS+Y*RHH*LL Q
Sbjct: 222 QYLLLV*HDLNHFD*IFALKIIPKVTPYRRGAV*NIANYFVCLLLQTHSKY*RHH*LLTQ 43
Query: 354 LYHLSHSWLHLGPD 313
LYHL HSWLH+GPD
Sbjct: 42 LYHLCHSWLHVGPD 1
Score = 111 bits (236), Expect = 9e-024
Identities = 51/73 (69%), Positives = 54/73 (73%)
Frame = -3 / -1
Query: 533 NTYRXCDXXXIXLIRXXXXKIIQKGLLIREEHXKILPTISSXCCFKPTQSIEGTISC*IN 354
NTY CD I LIR + QK LLI EE KILPTISS CCFKPTQSIEGTISC*+N
Sbjct: 221 NTYC*CDMI*ITLIRFSPSRSSQK*LLIGEEQFKILPTISSVCCFKPTQSIEGTISC*LN 42
Query: 353 STIFPIHGSI*DL 315
ST F IHGS+*DL
Sbjct: 41 STTFAIHGSM*DL 3
Score = 105 bits (223), Expect = 5e-022
Identities = 51/76 (67%), Positives = 54/76 (71%)
Frame = -1 / -2
Query: 541 VXXILIXSVTXXESX*SEXXXXRSSKRDSLSERSIXKYCQLFRXXAASNPLRVLKAPLVV 362
V ILI SVT ES * + K DSLSERS KYCQLFR AASNPL+VLKAPLVV
Sbjct: 229 VSSILIASVT*FESL*LDFRPQDHPKSDSLSERSSLKYCQLFRLSAASNPLKVLKAPLVV 50
Query: 361 ESTLPSFPFMAPFRT* 314
+STLP PFMAP RT*
Sbjct: 49 DSTLPPLPFMAPCRT* 2
>gb|CA621622.1|CA621622 wl1n.pk0075.g9 wl1n Triticum aestivum cDNA clone wl1n.pk0075.g9 5'
end, mRNA sequence
Length = 396
Score = 99.6 bits (211), Expect(2) = 5e-021
Identities = 41/55 (74%), Positives = 44/55 (80%)
Frame = -2 / -1
Query: 477 QDHPKGTPYQRGASXNIANYFVXXLLQTHSEY*RHH*LLNQLYHLSHSWLHLGPD 313
+DHPK RGA NIANYFV LLQTHS+Y*RHH*LL QLYHL HSWLH+GPD
Sbjct: 165 KDHPKVILIGRGAV*NIANYFVCLLLQTHSKY*RHH*LLTQLYHLCHSWLHVGPD 1
Score = 21.2 bits (40), Expect(2) = 5e-021
Identities = 11/28 (39%), Positives = 13/28 (46%)
Frame = -3 / -1
Query: 533 NTYRXCDXXXIXLIRXXXXKIIQKGLLI 450
N Y CD I L+R K K +LI
Sbjct: 222 NNYC*CDMVWITLVRIFALKDHPKVILI 139
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 420,602,670
Number of Sequences: 636343
Number of extensions: 5811095
Number of successful extensions: 75505
Number of sequences better than 1.0e-020: 11
length of database: 122,413,413
effective HSP length: 52
effective length of database: 89,323,577
effective search space used: 11344094279
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)