TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAS4f06.yg.3.1
(768 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA626862.1|CA626862 wl1n.pk0147.d10 wl1n Triticum aestiv... 417 e-115
gb|CA619430.1|CA619430 wl1n.pk0051.b8 wl1n Triticum aestivu... 143 1e-073
gb|CV766205.1|CV766205 FGAS060592 Triticum aestivum FGAS: L... 126 4e-043
>gb|CA626862.1|CA626862 wl1n.pk0147.d10 wl1n Triticum aestivum cDNA clone wl1n.pk0147.d10
5' end, mRNA sequence
Length = 585
Score = 417 bits (904), Expect = e-115
Identities = 146/158 (92%), Positives = 148/158 (93%)
Frame = -3 / -3
Query: 502 DCCWNRSWNGWQGSGNRGHWWQGNRDCRNSWYGGWNHRVWERWDCASSCWWQG*LWDRNG 323
DCCWNRSW GWQGSGNRGH + +WYGGWNHRVWERWDCASSCWWQG*LWDRNG
Sbjct: 475 DCCWNRSWXGWQGSGNRGHCGRVTGTAEIAWYGGWNHRVWERWDCASSCWWQG*LWDRNG 296
Query: 322 RDWWDCHLGDLRNGRHWRQRQRGWNRWYGRHWRQFGWNRWNGRLSRRGLWRSCCVQEMSS 143
RDWWDCHLGDLRNGRHWRQRQRGWNRWYGRHWRQFGWNRWNGRLSRRGLWRSCCVQEMSS
Sbjct: 295 RDWWDCHLGDLRNGRHWRQRQRGWNRWYGRHWRQFGWNRWNGRLSRRGLWRSCCVQEMSS 116
Query: 142 CKAAIEGSQD*EQGEKTATRSATSHGYHCVRICFGCIR 29
CKAAIEGSQD*EQGEKTATRSATSHGYHCVRICFGCIR
Sbjct: 115 CKAAIEGSQD*EQGEKTATRSATSHGYHCVRICFGCIR 2
Score = 376 bits (816), Expect = e-103
Identities = 149/159 (93%), Positives = 149/159 (93%)
Frame = +1 / +1
Query: 28 L*YTQSKYARSDSHGSWRIWLPSSRPAPSLDCLQWQLCSSTSLGHSSCARGHACSAVHSS 207
L*YTQSKYARSDSHGSWRIWLPSSRPAPSLDCLQWQLCSSTSLGHSSCARGHACSAVHSS
Sbjct: 1 L*YTQSKYARSDSHGSWRIWLPSSRPAPSLDCLQWQLCSSTSLGHSSCARGHACSAVHSS 180
Query: 208 GSNQTASSADHTSGSNHADAASNAFHSGGPQDDNPTNPVHSYPKASPATNSCWRNPISPK 387
GSNQTASSADHTSGSNHADAASNAFHSGGPQDDNPTNPVHSYPKASPATNSCWRNPISPK
Sbjct: 181 GSNQTASSADHTSGSNHADAASNAFHSGGPQDDNPTNPVHSYPKASPATNSCWRNPISPK 360
Query: 388 PGGSNHRTSYFCSPGYPATNALDSHYLAIHSNYDSNNNP 504
PGGSNHRT F P NALDSHYLAI SNYDSNNNP
Sbjct: 361 PGGSNHRTKLFLQSRLPCHNALDSHYLAIXSNYDSNNNP 477
Score = 255 bits (552), Expect(2) = 4e-080
Identities = 100/100 (100%), Positives = 100/100 (100%)
Frame = -2 / -2
Query: 329 EWTGLVGLSSWGPPEWKALEAASAWLEPLVWSALEAVWLEPLEWTAEQAWPLAQLLCPRD 150
EWTGLVGLSSWGPPEWKALEAASAWLEPLVWSALEAVWLEPLEWTAEQAWPLAQLLCPRD
Sbjct: 302 EWTGLVGLSSWGPPEWKALEAASAWLEPLVWSALEAVWLEPLEWTAEQAWPLAQLLCPRD 123
Query: 149 VELQSCH*RQSRLGAGREDGNQIRHEPWLSLRAYLLWVY* 30
VELQSCH*RQSRLGAGREDGNQIRHEPWLSLRAYLLWVY*
Sbjct: 122 VELQSCH*RQSRLGAGREDGNQIRHEPWLSLRAYLLWVY* 3
Score = 109 bits (232), Expect(3) = 7e-057
Identities = 47/65 (72%), Positives = 47/65 (72%)
Frame = +2 / +2
Query: 29 SNTPKANTHAVIAMARGGSGCRXXXXXXXXTAFNGSFAARHLLDXXXXXXXXXXQPSIPA 208
SNTPKANTHAVIAMARGGSGCR TAFNGSFAARHLLD QPSIPA
Sbjct: 2 SNTPKANTHAVIAMARGGSGCRLLALLLVLTAFNGSFAARHLLDTAAAPEATPAQPSIPA 181
Query: 209 VPTKL 223
VPTKL
Sbjct: 182 VPTKL 196
Score = 100 bits (213), Expect(4) = 4e-032
Identities = 44/44 (100%), Positives = 44/44 (100%)
Frame = +3 / +3
Query: 30 LIHPKQIRTQ**PWLVADLVAVFSPCS*S*LPSMAALQLDISWT 161
LIHPKQIRTQ**PWLVADLVAVFSPCS*S*LPSMAALQLDISWT
Sbjct: 3 LIHPKQIRTQ**PWLVADLVAVFSPCS*S*LPSMAALQLDISWT 134
Score = 87.2 bits (184), Expect(3) = 7e-057
Identities = 35/58 (60%), Positives = 36/58 (62%)
Frame = +3 / +3
Query: 330 LSQS*PCXXXXXXXXXXXXTRWFQPPYQLFLQSRLPCHQCPRFPLPCHPFQLRFQQQS 503
LSQS*PC TRWFQPPYQ + QCPRFPLPCHP QLRFQQQS
Sbjct: 303 LSQS*PCHQQLLAQSHLSQTRWFQPPYQAISAVPVTLPQCPRFPLPCHPXQLRFQQQS 476
Score = 71.6 bits (150), Expect(3) = 3e-024
Identities = 37/64 (57%), Positives = 37/64 (57%)
Frame = -1 / -1
Query: 219 LVGTAGMDXXXXXXXXXXXXSKRCRAAKLPLKAVKTXXXXXXXXXXXXXXXAITACVFAL 40
LVGTAGMD SKRCRAAKLPLKAVKT AITACVFAL
Sbjct: 192 LVGTAGMDG*AGVASGAAAVSKRCRAAKLPLKAVKTRSRARRRQPDPPRAMAITACVFAL 13
Query: 39 GVLE 28
GVLE
Sbjct: 12 GVLE 1
Score = 64.3 bits (134), Expect(3) = 7e-057
Identities = 23/23 (100%), Positives = 23/23 (100%)
Frame = +3 / +3
Query: 213 QPNCLQCRPYQRFQPR*RCLQCL 281
QPNCLQCRPYQRFQPR*RCLQCL
Sbjct: 186 QPNCLQCRPYQRFQPR*RCLQCL 254
Score = 63.8 bits (133), Expect(2) = 4e-080
Identities = 29/39 (74%), Positives = 30/39 (76%)
Frame = -1 / -1
Query: 453 EGIGGRVTGTAEIAGTVVGTTGFGRDGIAPAAVGGRASF 337
EGI G + GTVVGTTGFGRDGIAPAAVGGRASF
Sbjct: 426 EGIVAG*PGLQK*LGTVVGTTGFGRDGIAPAAVGGRASF 310
Score = 59.3 bits (123), Expect(3) = 3e-024
Identities = 25/34 (73%), Positives = 26/34 (76%)
Frame = -2 / -2
Query: 476 WMAR*WESRALVAG*PGLQK*LVRWLEPPGLGEM 375
WMAR*WESRAL G + LVRWLEPPGLGEM
Sbjct: 449 WMAR*WESRALWQGNRDCRNSLVRWLEPPGLGEM 348
Score = 49.2 bits (101), Expect(4) = 4e-032
Identities = 20/25 (80%), Positives = 21/25 (84%)
Frame = +2 / +2
Query: 356 TAAGAIPSLPNPVVPTTVPAISAVP 430
TAAGAIPSLPNPVVPTTVP+ P
Sbjct: 329 TAAGAIPSLPNPVVPTTVPSYFCSP 403
Score = 25.4 bits (49), Expect(4) = 4e-032
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +1 / +1
Query: 160 HSSCARGHACSAVHSSGSNQTASSAD 237
HSS + A SA H+SGSN ++++
Sbjct: 172 HSSGSNQTASSADHTSGSNHADAASN 249
Score = 21.7 bits (41), Expect(3) = 3e-024
Identities = 8/29 (27%), Positives = 11/29 (37%)
Frame = -3 / -3
Query: 400 WNHRVWERWDCASSCWWQG*LWDRNGRDW 314
W+ + WDC G W + R W
Sbjct: 310 WDRNGRDWWDCHLGDLRNGRHWRQRQRGW 224
Score = 21.7 bits (41), Expect(4) = 4e-032
Identities = 6/9 (66%), Positives = 8/9 (88%)
Frame = +3 / +3
Query: 222 CLQCRPYQR 248
CLQC P++R
Sbjct: 240 CLQCLPFRR 266
>gb|CA619430.1|CA619430 wl1n.pk0051.b8 wl1n Triticum aestivum cDNA clone wl1n.pk0051.b8 5'
end, mRNA sequence
Length = 620
Score = 143 bits (306), Expect(6) = 6e-071
Identities = 55/56 (98%), Positives = 56/56 (100%)
Frame = +1 / +1
Query: 28 L*YTQSKYARSDSHGSWRIWLPSSRPAPSLDCLQWQLCSSTSLGHSSCARGHACSA 195
L*YTQSKYARSDSHGSWRIWLPSSRPAPSLDCLQWQLCSSTSLGHSSCARGHACS+
Sbjct: 1 L*YTQSKYARSDSHGSWRIWLPSSRPAPSLDCLQWQLCSSTSLGHSSCARGHACSS 168
Score = 139 bits (298), Expect(4) = 1e-073
Identities = 55/55 (100%), Positives = 55/55 (100%)
Frame = -3 / -2
Query: 193 LSRRGLWRSCCVQEMSSCKAAIEGSQD*EQGEKTATRSATSHGYHCVRICFGCIR 29
LSRRGLWRSCCVQEMSSCKAAIEGSQD*EQGEKTATRSATSHGYHCVRICFGCIR
Sbjct: 166 LSRRGLWRSCCVQEMSSCKAAIEGSQD*EQGEKTATRSATSHGYHCVRICFGCIR 2
Score = 136 bits (291), Expect(4) = 2e-059
Identities = 54/54 (100%), Positives = 54/54 (100%)
Frame = -2 / -1
Query: 191 EQAWPLAQLLCPRDVELQSCH*RQSRLGAGREDGNQIRHEPWLSLRAYLLWVY* 30
EQAWPLAQLLCPRDVELQSCH*RQSRLGAGREDGNQIRHEPWLSLRAYLLWVY*
Sbjct: 164 EQAWPLAQLLCPRDVELQSCH*RQSRLGAGREDGNQIRHEPWLSLRAYLLWVY* 3
Score = 104 bits (222), Expect(4) = 1e-073
Identities = 34/36 (94%), Positives = 34/36 (94%)
Frame = -3 / -3
Query: 352 WQG*LWDRNGRDWWDCHLGDLRNGRHWRQRQRGWNR 245
WQG WDRNGRDWWDCHLGDLRNGRHWRQRQRGWNR
Sbjct: 327 WQGSFWDRNGRDWWDCHLGDLRNGRHWRQRQRGWNR 220
Score = 100 bits (213), Expect(4) = 5e-031
Identities = 44/44 (100%), Positives = 44/44 (100%)
Frame = +3 / +3
Query: 30 LIHPKQIRTQ**PWLVADLVAVFSPCS*S*LPSMAALQLDISWT 161
LIHPKQIRTQ**PWLVADLVAVFSPCS*S*LPSMAALQLDISWT
Sbjct: 3 LIHPKQIRTQ**PWLVADLVAVFSPCS*S*LPSMAALQLDISWT 134
Score = 90.9 bits (192), Expect(6) = 6e-071
Identities = 34/38 (89%), Positives = 36/38 (94%)
Frame = +1 / +3
Query: 247 GSNHADAASNAFHSGGPQDDNPTNPVHSYPKASPATNS 360
GSNHADAASNAFHSGGPQDDNPTNPVHSYPK+ PAT +
Sbjct: 222 GSNHADAASNAFHSGGPQDDNPTNPVHSYPKSYPATTA 335
Score = 76.7 bits (161), Expect(4) = 2e-059
Identities = 28/28 (100%), Positives = 28/28 (100%)
Frame = -2 / -2
Query: 329 EWTGLVGLSSWGPPEWKALEAASAWLEP 246
EWTGLVGLSSWGPPEWKALEAASAWLEP
Sbjct: 304 EWTGLVGLSSWGPPEWKALEAASAWLEP 221
Score = 60.2 bits (125), Expect(4) = 1e-073
Identities = 18/20 (90%), Positives = 19/20 (95%)
Frame = -3 / -1
Query: 244 WYGRHWRQFGWNRWNGRLSR 185
WYGRHWRQFGWNRWNGRL +
Sbjct: 218 WYGRHWRQFGWNRWNGRLEQ 159
Score = 46.9 bits (96), Expect(4) = 2e-059
Identities = 17/17 (100%), Positives = 17/17 (100%)
Frame = -2 / -3
Query: 242 VWSALEAVWLEPLEWTA 192
VWSALEAVWLEPLEWTA
Sbjct: 216 VWSALEAVWLEPLEWTA 166
Score = 41.8 bits (85), Expect(6) = 6e-071
Identities = 17/17 (100%), Positives = 17/17 (100%)
Frame = +1 / +2
Query: 193 AVHSSGSNQTASSADHT 243
AVHSSGSNQTASSADHT
Sbjct: 167 AVHSSGSNQTASSADHT 217
Score = 33.6 bits (67), Expect(6) = 6e-071
Identities = 12/14 (85%), Positives = 13/14 (92%)
Frame = +1 / +1
Query: 376 ISPKPGGSNHRTSY 417
ISPKPGGSN+RT Y
Sbjct: 349 ISPKPGGSNNRTXY 390
Score = 32.7 bits (65), Expect(4) = 5e-031
Identities = 12/12 (100%), Positives = 12/12 (100%)
Frame = +3 / +2
Query: 246 RFQPR*RCLQCL 281
RFQPR*RCLQCL
Sbjct: 221 RFQPR*RCLQCL 256
Score = 32.2 bits (64), Expect(4) = 5e-031
Identities = 10/10 (100%), Positives = 10/10 (100%)
Frame = +3 / +1
Query: 213 QPNCLQCRPY 242
QPNCLQCRPY
Sbjct: 187 QPNCLQCRPY 216
Score = 30.9 bits (61), Expect(4) = 1e-073
Identities = 10/15 (66%), Positives = 10/15 (66%)
Frame = -3 / -2
Query: 418 NSWYGGWNHRVWERW 374
N YG NH VWERW
Sbjct: 391 NXXYGCLNHXVWERW 347
Score = 27.6 bits (54), Expect(4) = 2e-059
Identities = 11/17 (64%), Positives = 13/17 (76%)
Frame = -2 / -1
Query: 425 LQK*LVRWLEPPGLGEM 375
+ +* VR EPPGLGEM
Sbjct: 398 IXE*XVRLFEPPGLGEM 348
Score = 26.7 bits (52), Expect(6) = 6e-071
Identities = 10/26 (38%), Positives = 13/26 (50%)
Frame = +3 / +2
Query: 444 QCPRFPLPCHPFQLRFQQQSLPFPDS 521
QCPRF LPC ++P P +
Sbjct: 413 QCPRFQLPCXXSNTIQXNPTIPDPSA 490
Score = 25.8 bits (50), Expect(6) = 6e-071
Identities = 8/13 (61%), Positives = 8/13 (61%)
Frame = +1 / +2
Query: 346 PATNSCWRNPISP 384
P NSCW NP P
Sbjct: 320 PCHNSCWXNPSLP 358
Score = 25.4 bits (49), Expect(4) = 5e-031
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +2 / +2
Query: 374 PSLPNPVVPTTVPAI 418
PSLPN VV TTV I
Sbjct: 347 PSLPNXVVQTTVXXI 391
>gb|CV766205.1|CV766205 FGAS060592 Triticum aestivum FGAS: Library 2 Gate 3 Triticum
aestivum cDNA, mRNA sequence
Length = 856
Score = 126 bits (270), Expect(4) = 4e-043
Identities = 47/95 (49%), Positives = 54/95 (56%)
Frame = -3 / -2
Query: 550 WGWGGEWRHLESGNGRDCCWNRSWNGWQGSGNRGHWWQGNRDCRNSWYGGWNHRVWERWD 371
WG HLE NGRD CW NGW G+ N GHWW GNR RN +GGW+ R +RW+
Sbjct: 585 WGHEWNRWHLEPRNGRDSCWYCDRNGWNGNWN*GHWWHGNRQSRNGRHGGWDRRNRKRWN 406
Query: 370 CASSCWWQG*LWDRNGRDWWDCHLGDLRNGRHWRQ 266
CA W G L DRN W C + NGRHWR+
Sbjct: 405 CARGGWCHGDLRDRNEWYWRHCQFRNCGNGRHWRR 301
Score = 105 bits (223), Expect(3) = 1e-028
Identities = 43/98 (43%), Positives = 58/98 (59%)
Frame = +1 / +3
Query: 262 DAASNAFHSGGPQDDNPTNPVHSYPKASPATNSCWRNPISPKPGGSNHRTSYFCSPGYPA 441
+ ASNAFHS + DN N +HSYP+ TN W + IS G NH + Y A
Sbjct: 297 NTASNAFHSRSSETDNAANTIHSYPEGRHGTNRPWHSSISSDSGDPNHHADHSYFACYHA 476
Query: 442 TNALDSHYLAIHSNYDSNNNPYHSRTPGASIHRPIPTN 555
TNAL S+Y +IHS++++NN PYHS P A+ +PT+
Sbjct: 477 TNALSSNYHSIHSDHNTNNYPYHSWVPDATYSIHVPTS 590
Score = 72.5 bits (152), Expect(2) = 4e-021
Identities = 29/51 (56%), Positives = 34/51 (66%)
Frame = +3 / +2
Query: 399 QPPYQLFLQSRLPCHQCPRFPLPCHPFQLRFQQQSLPFPDSRCLHSPPHPH 551
QPP + FL LPCHQCP+F LP HPF+ ++QQ SLPF SRC PH
Sbjct: 434 QPPCRPFLLCLLPCHQCPQFQLPFHPFRSQYQQLSLPFLGSRCHLFHSCPH 586
Score = 56.1 bits (116), Expect(5) = 1e-022
Identities = 23/39 (58%), Positives = 25/39 (64%)
Frame = -1 / -3
Query: 453 EGIGGRVTGTAEIAGTVVGTTGFGRDGIAPAAVGGRASF 337
EGIGG VTG + G VVG G GRDG P AVG A+F
Sbjct: 488 EGIGGMVTGKVGMVGMVVGIAGIGRDGTVPGAVGAMATF 372
Score = 51.9 bits (107), Expect(2) = 4e-021
Identities = 21/43 (48%), Positives = 28/43 (65%)
Frame = +1 / +3
Query: 145 STSLGHSSCARGHACSAVHSSGSNQTASSADHTSGSNHADAAS 273
S+S GH SC RGH SA ++ SN TAS+A H+ S +AA+
Sbjct: 225 SSSFGHGSCTRGHTSSAFYTGSSNNTASNAFHSRSSETDNAAN 353
Score = 42.3 bits (86), Expect(4) = 4e-043
Identities = 18/33 (54%), Positives = 21/33 (63%)
Frame = -2 / -1
Query: 239 WSALEAVWLEPLEWTAEQAWPLAQLLCPRDVEL 141
W ALEAV LE AE WPL QL CP++ E+
Sbjct: 319 WKALEAVLLELPV*NAELVWPLVQLPCPKEDEM 221
Score = 41.4 bits (84), Expect(5) = 1e-022
Identities = 17/36 (47%), Positives = 22/36 (61%)
Frame = -3 / -2
Query: 238 GRHWRQFGWNRWNGRLSRRGLWRSCCVQEMSSCKAA 131
GRHWR+ W+ LS GLW SC VQ+ + C+ A
Sbjct: 318 GRHWRRCCWSFRCRTLSWCGLWCSCRVQKRTRCRRA 211
Score = 34.5 bits (69), Expect(4) = 4e-043
Identities = 15/26 (57%), Positives = 17/26 (65%)
Frame = -1 / -3
Query: 606 NSM*RKVSCYIELQGLVVCGDGAVNG 529
+S * VSCY GLVVCG G +NG
Sbjct: 647 SSP*LAVSCYDGFHGLVVCGGGDMNG 570
Score = 32.2 bits (64), Expect(3) = 1e-028
Identities = 13/21 (61%), Positives = 14/21 (66%)
Frame = +1 / +3
Query: 91 PSSRPAPSLDCLQWQLCSSTS 153
PS APSL LQWQLC +S
Sbjct: 168 PSPCAAPSLHSLQWQLCGISS 230
Score = 30.9 bits (61), Expect(5) = 1e-022
Identities = 13/20 (65%), Positives = 14/20 (70%)
Frame = -3 / -2
Query: 589 SLLLHRTSRAGGLWGWGGEW 530
SL+L R S AGGL WG EW
Sbjct: 630 SLML*RLSWAGGLRRWGHEW 571
Score = 28.6 bits (56), Expect(4) = 4e-043
Identities = 12/17 (70%), Positives = 13/17 (76%)
Frame = -2 / -1
Query: 140 QSCH*RQSRLGAGREDG 90
QSCH*R RLGA + DG
Sbjct: 217 QSCH*RL*RLGAAQGDG 167
Score = 28.1 bits (55), Expect(3) = 1e-028
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = +1 / +3
Query: 529 SIHRPIPTNHQPLKFDVAGD 588
SIH P NHQP+K +A D
Sbjct: 570 SIHVPTSANHQPMKAVIA*D 629
Score = 28.1 bits (55), Expect(5) = 1e-022
Identities = 16/30 (53%), Positives = 17/30 (56%)
Frame = -2 / -1
Query: 527 APGVREW*GXXXXXXXEWMAR*WESRALVA 438
A G +EW*G EWM * E RALVA
Sbjct: 562 ASGTQEW*G*LLVL*SEWMEW*LELRALVA 473
Score = 22.1 bits (42), Expect(5) = 1e-022
Identities = 9/10 (90%), Positives = 10/10 (100%)
Frame = -1 / -3
Query: 144 AAKLPLKAVK 115
AA+LPLKAVK
Sbjct: 221 AAELPLKAVK 192
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 914,213,180
Number of Sequences: 636343
Number of extensions: 20076157
Number of successful extensions: 288935
Number of sequences better than 1.0e-020: 6
length of database: 122,413,413
effective HSP length: 53
effective length of database: 88,687,234
effective search space used: 17914821268
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)