TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAJ1a04.yg.2.1
(386 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA612779.1|CA612779 wr1.pk0143.e6 wr1 Triticum aestivum ... 100 2e-029
gb|CB307317.1|CB307317 HFIG302 Hessian fly infested cDNA li... 98 3e-028
gb|BJ226024.1|BJ226024 BJ226024 Y. Ogihara unpublished cDNA... 98 3e-028
gb|CA601536.1|CA601536 wr1.pk0004.e12 wr1 Triticum aestivum... 98 3e-028
gb|BF293059.1|BF293059 WHE2160_B02_C04ZS Triticum turgidum ... 98 3e-028
gb|CB307758.1|CB307758 HFIG743 Hessian fly infested cDNA li... 95 2e-027
gb|CA632146.1|CA632146 wle1n.pk0049.g9 wle1n Triticum aesti... 100 4e-021
>gb|CA612779.1|CA612779 wr1.pk0143.e6 wr1 Triticum aestivum cDNA clone wr1.pk0143.e6 5'
end, mRNA sequence
Length = 502
Score = 100 bits (214), Expect(2) = 2e-029
Identities = 38/80 (47%), Positives = 48/80 (60%)
Frame = +3 / +1
Query: 144 NXYKNFAKXXXXNFXXKXXNWXXFNXPXXFTSFSYGTGVFXPGRCSPGLDWAIPTGNSLX 323
N YK+FAK +F + NW FN P + FSYG G+ PGRCSPG+D A+P G+SL
Sbjct: 151 NDYKHFAKVCFESFGDRVKNWFTFNEPHTYCCFSYGEGIHAPGRCSPGMDCAVPXGDSLK 330
Query: 324 XPYIGXXNILLAHXXXXXLY 383
PY +ILLAH L+
Sbjct: 331 EPYTAGQHILLAHAEAVELF 390
Score = 46.9 bits (96), Expect(2) = 2e-029
Identities = 17/38 (44%), Positives = 22/38 (57%)
Frame = +3 / +1
Query: 12 YKXXXNXXXXNGIEPYXTLFHWDTPQXLVDXXXXFLDK 125
Y N +GI PY T++HWDTPQ L D FL++
Sbjct: 28 YNNLINSLIHHGIVPYVTIWHWDTPQALEDKYGGFLNR 141
>gb|CB307317.1|CB307317 HFIG302 Hessian fly infested cDNA library Triticum aestivum cDNA,
mRNA sequence
Length = 506
Score = 98.2 bits (208), Expect(2) = 3e-028
Identities = 37/78 (47%), Positives = 46/78 (58%)
Frame = +3 / +1
Query: 150 YKNFAKXXXXNFXXKXXNWXXFNXPXXFTSFSYGTGVFXPGRCSPGLDWAIPTGNSLXXP 329
YK FA+ NF + NW FN P + FSYG G+ PGRCSPG+D A+P G+SL P
Sbjct: 142 YKQFAEVCFKNFGDRVKNWFTFNEPHTYCCFSYGEGIHAPGRCSPGMDCAVPEGDSLREP 321
Query: 330 YIGXXNILLAHXXXXXLY 383
Y +ILLAH L+
Sbjct: 322 YTAGHHILLAHAEAVQLF 375
Score = 46.0 bits (94), Expect(2) = 3e-028
Identities = 17/38 (44%), Positives = 21/38 (55%)
Frame = +3 / +1
Query: 12 YKXXXNXXXXNGIEPYXTLFHWDTPQXLVDXXXXFLDK 125
Y N N I PY T++HWDTPQ L D FL++
Sbjct: 13 YNKLINSLIDNDIVPYVTIWHWDTPQALEDKYGGFLNR 126
>gb|BJ226024.1|BJ226024 BJ226024 Y. Ogihara unpublished cDNA library, Wh_dL Triticum
aestivum cDNA clone whdl24m14 5', mRNA sequence
Length = 527
Score = 98.2 bits (208), Expect(2) = 3e-028
Identities = 37/78 (47%), Positives = 46/78 (58%)
Frame = +3 / +1
Query: 150 YKNFAKXXXXNFXXKXXNWXXFNXPXXFTSFSYGTGVFXPGRCSPGLDWAIPTGNSLXXP 329
YK FA+ NF + NW FN P + FSYG G+ PGRCSPG+D A+P G+SL P
Sbjct: 151 YKQFAEVCFKNFGDRVKNWFTFNEPHTYCCFSYGEGIHAPGRCSPGMDCAVPKGDSLREP 330
Query: 330 YIGXXNILLAHXXXXXLY 383
Y +ILLAH L+
Sbjct: 331 YTAGHHILLAHAEAVELF 384
Score = 46.0 bits (94), Expect(2) = 3e-028
Identities = 18/38 (47%), Positives = 22/38 (57%)
Frame = +3 / +1
Query: 12 YKXXXNXXXXNGIEPYXTLFHWDTPQXLVDXXXXFLDK 125
Y N N I PY T++HWDTPQ L D FL++
Sbjct: 22 YNKLXNSLIDNDIVPYVTIWHWDTPQALEDKYGGFLNR 135
>gb|CA601536.1|CA601536 wr1.pk0004.e12 wr1 Triticum aestivum cDNA clone wr1.pk0004.e12 5'
end, mRNA sequence
Length = 547
Score = 98.2 bits (208), Expect(2) = 3e-028
Identities = 37/78 (47%), Positives = 46/78 (58%)
Frame = +3 / +2
Query: 150 YKNFAKXXXXNFXXKXXNWXXFNXPXXFTSFSYGTGVFXPGRCSPGLDWAIPTGNSLXXP 329
YK FA+ NF + NW FN P + FSYG G+ PGRCSPG+D A+P G+SL P
Sbjct: 173 YKQFAEVCFKNFGDRVKNWFTFNEPHTYCCFSYGEGIHAPGRCSPGMDCAVPKGDSLREP 352
Query: 330 YIGXXNILLAHXXXXXLY 383
Y +ILLAH L+
Sbjct: 353 YTAGHHILLAHAEAVELF 406
Score = 46.0 bits (94), Expect(2) = 3e-028
Identities = 17/38 (44%), Positives = 21/38 (55%)
Frame = +3 / +2
Query: 12 YKXXXNXXXXNGIEPYXTLFHWDTPQXLVDXXXXFLDK 125
Y N N I PY T++HWDTPQ L D FL++
Sbjct: 44 YNKLINSLIDNDIVPYVTIWHWDTPQALEDKYGGFLNR 157
>gb|BF293059.1|BF293059 WHE2160_B02_C04ZS Triticum turgidum L. var. durum (durum wheat)
whole plant cDNA library Triticum turgidum cDNA clone
WHE2160_B02_C04, mRNA sequence
Length = 600
Score = 98.2 bits (208), Expect(2) = 3e-028
Identities = 37/78 (47%), Positives = 46/78 (58%)
Frame = +3 / +2
Query: 150 YKNFAKXXXXNFXXKXXNWXXFNXPXXFTSFSYGTGVFXPGRCSPGLDWAIPTGNSLXXP 329
YK FA+ NF + NW FN P + FSYG G+ PGRCSPG+D A+P G+SL P
Sbjct: 158 YKQFAEVCFKNFGDRVKNWFTFNEPHTYCCFSYGEGIHAPGRCSPGMDCAVPKGDSLREP 337
Query: 330 YIGXXNILLAHXXXXXLY 383
Y +ILLAH L+
Sbjct: 338 YTAGHHILLAHAEAVELF 391
Score = 46.0 bits (94), Expect(2) = 3e-028
Identities = 17/38 (44%), Positives = 21/38 (55%)
Frame = +3 / +2
Query: 12 YKXXXNXXXXNGIEPYXTLFHWDTPQXLVDXXXXFLDK 125
Y N N I PY T++HWDTPQ L D FL++
Sbjct: 29 YNKLINSLIDNDIVPYVTIWHWDTPQALEDKYGGFLNR 142
>gb|CB307758.1|CB307758 HFIG743 Hessian fly infested cDNA library Triticum aestivum cDNA,
mRNA sequence
Length = 645
Score = 95.0 bits (201), Expect(2) = 2e-027
Identities = 36/73 (49%), Positives = 45/73 (61%)
Frame = +3 / +2
Query: 144 NXYKNFAKXXXXNFXXKXXNWXXFNXPXXFTSFSYGTGVFXPGRCSPGLDWAIPTGNSLX 323
N YK+FAK +F + NW FN P + FSYG G+ PGRCSPG+D A+P +SL
Sbjct: 419 NDYKHFAKVCFESFGDRVKNWFTFNEPHTYCCFSYGEGIHAPGRCSPGMDCAVPERDSLR 598
Query: 324 XPYIGXXNILLAH 362
PY +ILLAH
Sbjct: 599 EPYTAGHHILLAH 637
Score = 46.9 bits (96), Expect(2) = 2e-027
Identities = 17/38 (44%), Positives = 22/38 (57%)
Frame = +3 / +2
Query: 12 YKXXXNXXXXNGIEPYXTLFHWDTPQXLVDXXXXFLDK 125
Y N +GI PY T++HWDTPQ L D FL++
Sbjct: 296 YNNLINSLIHHGIVPYVTIWHWDTPQALEDKYGGFLNR 409
>gb|CA632146.1|CA632146 wle1n.pk0049.g9 wle1n Triticum aestivum cDNA clone wle1n.pk0049.g9
5' end, mRNA sequence
Length = 574
Score = 100 bits (213), Expect(2) = 4e-021
Identities = 38/80 (47%), Positives = 48/80 (60%)
Frame = +3 / +1
Query: 144 NXYKNFAKXXXXNFXXKXXNWXXFNXPXXFTSFSYGTGVFXPGRCSPGLDWAIPTGNSLX 323
N YK+FAK +F + NW FN P + FSYG G+ PGRCSPG+D A+P G+SL
Sbjct: 61 NDYKHFAKVCFESFGDRVKNWFTFNEPHTYCCFSYGEGIHAPGRCSPGMDCAVPEGDSLR 240
Query: 324 XPYIGXXNILLAHXXXXXLY 383
PY +ILLAH L+
Sbjct: 241 EPYTAGHHILLAHAEAVELF 300
Score = 19.9 bits (37), Expect(2) = 4e-021
Identities = 9/16 (56%), Positives = 9/16 (56%)
Frame = +1 / +2
Query: 76 GTHLXXWWTXTXXS*I 123
GT L W T T S*I
Sbjct: 2 GTPLKHWKTSTAVS*I 49
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 203,176,728
Number of Sequences: 636343
Number of extensions: 1469117
Number of successful extensions: 71836
Number of sequences better than 1.0e-020: 10
length of database: 122,413,413
effective HSP length: 51
effective length of database: 89,959,920
effective search space used: 6926913840
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)