TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF1g01.yg.2.1
(335 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CF133118.1|CF133118 WHE4354_C05_F10ZT Wheat meiotic flor... 190 6e-048
gb|BQ902766.1|BQ902766 Ta03_01c07_R Ta03_AAFC_ECORC_Fusariu... 187 4e-047
gb|DR736451.1|DR736451 FGAS081821 Triticum aestivum FGAS: L... 147 4e-035
gb|CA501219.1|CA501219 WHE4030_G09_M18ZT Wheat meiotic anth... 133 5e-031
gb|CA497978.1|CA497978 WHE3236_G04_N08ZT Wheat meiotic anth... 117 5e-026
gb|BJ268450.1|BJ268450 BJ268450 Y. Ogihara unpublished cDNA... 115 2e-025
>gb|CF133118.1|CF133118 WHE4354_C05_F10ZT Wheat meiotic floret cDNA library Triticum
aestivum cDNA clone WHE4354_C05_F10, mRNA sequence
Length = 779
Score = 190 bits (409), Expect = 6e-048
Identities = 79/88 (89%), Positives = 82/88 (93%)
Frame = -2 / +1
Query: 265 KVRXXYXXPDVFDRIFXITRGGIXKASRVINISEDIYAGFNSTLRQGNITHHEYIQVGKG 86
KVR Y PDVFDRIF ITRGGI KASR+INISEDI+AGFNSTLRQGNITHHEYIQVGKG
Sbjct: 73 KVRMHYGHPDVFDRIFHITRGGISKASRIINISEDIFAGFNSTLRQGNITHHEYIQVGKG 252
Query: 85 RDVGLNQIALFEGKVAGGNGEQVLSRDV 2
RDVGLNQIALFEGKVAGGNGEQVLSRD+
Sbjct: 253 RDVGLNQIALFEGKVAGGNGEQVLSRDI 336
Score = 135 bits (289), Expect = 2e-031
Identities = 59/79 (74%), Positives = 61/79 (77%)
Frame = +3 / -1
Query: 6 SRLRTCSPLPPATFPSNSAIWFKPTSRPFPTWIYSWCVMFPWRKVELNPAYISSLILITR 185
S LRTCSP PPATFPSNSAI PTS P PTWIYSW VMFP R+VELNPA ISSLIL+ R
Sbjct: 332 SLLRTCSPFPPATFPSNSAI*LSPTSLPLPTWIYSW*VMFP*RRVELNPANISSLILMIR 153
Query: 186 DAXXMPPRVXXNIRSKTSG 242
DA MPP V NI S TSG
Sbjct: 152 DALLMPPLVI*NILSNTSG 96
>gb|BQ902766.1|BQ902766 Ta03_01c07_R
Ta03_AAFC_ECORC_Fusarium_graminearum_inoculated_wheat_he
ads Triticum aestivum cDNA clone Ta03_01c07, mRNA
sequence
Length = 559
Score = 187 bits (403), Expect = 4e-047
Identities = 78/88 (88%), Positives = 81/88 (92%)
Frame = -2 / -2
Query: 265 KVRXXYXXPDVFDRIFXITRGGIXKASRVINISEDIYAGFNSTLRQGNITHHEYIQVGKG 86
KVR Y PDVFDRIF ITRGGI KASR+INISEDI+AGFNSTLRQGNITHHEYIQVGKG
Sbjct: 339 KVRMHYGHPDVFDRIFHITRGGISKASRIINISEDIFAGFNSTLRQGNITHHEYIQVGKG 160
Query: 85 RDVGLNQIALFEGKVAGGNGEQVLSRDV 2
RDVGLNQIALFEGKVAGGN EQVLSRD+
Sbjct: 159 RDVGLNQIALFEGKVAGGNAEQVLSRDI 76
Score = 124 bits (266), Expect(2) = 3e-028
Identities = 57/79 (72%), Positives = 59/79 (74%)
Frame = +3 / +2
Query: 6 SRLRTCSPLPPATFPSNSAIWFKPTSRPFPTWIYSWCVMFPWRKVELNPAYISSLILITR 185
S LRTCS PPATFPSNSAI PTS P PT IYSW VMFP R+VELNPA ISSLIL+ R
Sbjct: 80 SLLRTCSAFPPATFPSNSAI*LSPTSLPLPT*IYSW*VMFP*RRVELNPANISSLILMIR 259
Query: 186 DAXXMPPRVXXNIRSKTSG 242
DA MPP V NI S TSG
Sbjct: 260 DALLMPPLVI*NILSNTSG 316
Score = 18.5 bits (34), Expect(2) = 3e-028
Identities = 8/16 (50%), Positives = 8/16 (50%)
Frame = +3 / +2
Query: 285 CPXXTKLXXXXXINXA 332
CP TKL IN A
Sbjct: 362 CPNVTKLVS*FDINEA 409
>gb|DR736451.1|DR736451 FGAS081821 Triticum aestivum FGAS: Library 5 GATE 7 Triticum
aestivum cDNA, mRNA sequence
Length = 1087
Score = 147 bits (316), Expect = 4e-035
Identities = 58/78 (74%), Positives = 69/78 (88%)
Frame = -2 / +1
Query: 235 VFDRIFXITRGGIXKASRVINISEDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIAL 56
VF R++ +TRGG+ KASRVINISEDI+ GFN TLR GN++HHEYIQVGKGRDVGLNQI++
Sbjct: 16 VFXRLWFLTRGGLSKASRVINISEDIFPGFNCTLRGGNVSHHEYIQVGKGRDVGLNQISM 195
Query: 55 FEGKVAGGNGEQVLSRDV 2
FE KV+ GNGEQ LSRD+
Sbjct: 196 FEAKVSSGNGEQTLSRDI 249
>gb|CA501219.1|CA501219 WHE4030_G09_M18ZT Wheat meiotic anther cDNA library Triticum
aestivum cDNA clone WHE4030_G09_M18, mRNA sequence
Length = 522
Score = 133 bits (286), Expect = 5e-031
Identities = 48/52 (92%), Positives = 50/52 (96%)
Frame = +3 / +2
Query: 3 TSRLRTCSPLPPATFPSNSAIWFKPTSRPFPTWIYSWCVMFPWRKVELNPAY 158
TSRLRTCSPLPPATFPSN AIWFKPTSRPFPTWIYSWCV+ PWR+VELNPAY
Sbjct: 179 TSRLRTCSPLPPATFPSNRAIWFKPTSRPFPTWIYSWCVILPWRRVELNPAY 334
Score = 126 bits (270), Expect = 8e-029
Identities = 52/52 (100%), Positives = 52/52 (100%)
Frame = -2 / -1
Query: 157 YAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDV 2
YAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDV
Sbjct: 333 YAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDV 178
>gb|CA497978.1|CA497978 WHE3236_G04_N08ZT Wheat meiotic anther cDNA library Triticum
aestivum cDNA clone WHE3236_G04_N08, mRNA sequence
Length = 633
Score = 117 bits (250), Expect = 5e-026
Identities = 48/56 (85%), Positives = 50/56 (89%)
Frame = -2 / +3
Query: 265 KVRXXYXXPDVFDRIFXITRGGIXKASRVINISEDIYAGFNSTLRQGNITHHEYIQ 98
KVR Y PDVFDRIF ITRGGI KASR+INISEDI+AGFNSTLRQGNITHHEYIQ
Sbjct: 462 KVRMHYGHPDVFDRIFHITRGGISKASRIINISEDIFAGFNSTLRQGNITHHEYIQ 629
>gb|BJ268450.1|BJ268450 BJ268450 Y. Ogihara unpublished cDNA library, Wh_oh Triticum
aestivum cDNA clone whoh17b01 5', mRNA sequence
Length = 425
Score = 115 bits (246), Expect = 2e-025
Identities = 47/48 (97%), Positives = 48/48 (100%)
Frame = -2 / +2
Query: 145 NSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDV 2
NSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRD+
Sbjct: 23 NSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDI 166
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 244,761,935
Number of Sequences: 636343
Number of extensions: 3384989
Number of successful extensions: 228796
Number of sequences better than 1.0e-020: 7
length of database: 122,413,413
effective HSP length: 50
effective length of database: 90,596,263
effective search space used: 5526372043
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)