TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3995470.2.1
(744 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BJ300649.1|BJ300649 BJ300649 Y. Ogihara unpublished cDNA... 133 2e-030
gb|CA731498.1|CA731498 wip1c.pk006.f21 wip1c Triticum aesti... 95 2e-025
gb|BG262604.1|BG262604 WHE0938_B02_C04ZS Wheat 5-15 DAP spi... 87 6e-023
gb|CA730249.1|CA730249 wip1c.pk003.b11 wip1c Triticum aesti... 87 6e-023
gb|BJ212167.1|BJ212167 BJ212167 Y. Ogihara unpublished cDNA... 87 6e-023
>gb|BJ300649.1|BJ300649 BJ300649 Y. Ogihara unpublished cDNA library, Wh_yd Triticum
aestivum cDNA clone whyd3i08 5', mRNA sequence
Length = 633
Score = 133 bits (286), Expect = 2e-030
Identities = 58/98 (59%), Positives = 71/98 (72%)
Frame = +2 / +2
Query: 89 RRGRISDDEINELISKLQALLPEXXXXXXXXXXXXXKLLKETCAYVKSLHREVDDLSERL 268
RRGRI+D+EINELISKLQAL+PE KLLKETC Y+KSLH+EV+DLS+RL
Sbjct: 143 RRGRITDEEINELISKLQALVPESSRRRSASRSSASKLLKETCGYIKSLHQEVEDLSDRL 322
Query: 269 SGLMATMDNDSPQAEIIRSLLR*LQVQVPAMPLAAPVV 382
S LM+T+D SPQAEIIR LLR* +P P+ + +
Sbjct: 323 SELMSTLDETSPQAEIIRGLLR*PDFILPIEPVGSSYI 436
Score = 55.1 bits (114), Expect(3) = 1e-023
Identities = 24/37 (64%), Positives = 30/37 (81%)
Frame = -3 / -3
Query: 331 EEAPDDLGLGAVVVHGRHEPRQPLREVVHLPVQALDV 221
E+APDDLGLGA +V GRH R+P+ EV+ L V+ALDV
Sbjct: 385 EKAPDDLGLGAGLVQGRH*LREPVGEVLDLLVEALDV 275
Score = 48.3 bits (99), Expect(3) = 1e-023
Identities = 19/23 (82%), Positives = 19/23 (82%)
Frame = -2 / -2
Query: 200 ASTPTTGSRSGGVRIPGGEPGAW 132
ASTPTTGSRSGG IPG PGAW
Sbjct: 254 ASTPTTGSRSGGGMIPGPAPGAW 186
Score = 44.6 bits (91), Expect(3) = 1e-023
Identities = 17/24 (70%), Positives = 22/24 (91%)
Frame = -3 / -3
Query: 157 FREESLELGDQLVDLVVADPATSA 86
FR++ LELGD+LVDL+V DPAT+A
Sbjct: 211 FRDQRLELGDELVDLLVGDPATAA 140
>gb|CA731498.1|CA731498 wip1c.pk006.f21 wip1c Triticum aestivum cDNA clone wip1c.pk006.f21
5' end, mRNA sequence
Length = 626
Score = 95.5 bits (202), Expect(2) = 2e-025
Identities = 40/48 (83%), Positives = 46/48 (95%)
Frame = +2 / +3
Query: 197 KLLKETCAYVKSLHREVDDLSERLSGLMATMDNDSPQAEIIRSLLR*L 340
K+LKETC+Y+KSLHREVDDLS+RLS LM+TMDN+SP AEIIRSLLR*L
Sbjct: 225 KMLKETCSYIKSLHREVDDLSDRLSDLMSTMDNNSPAAEIIRSLLR*L 368
Score = 40.9 bits (83), Expect(2) = 2e-025
Identities = 17/27 (62%), Positives = 21/27 (77%)
Frame = +2 / +3
Query: 74 MSSGGRRGRISDDEINELISKLQALLP 154
MS RG +S++EINELIS+LQ LLP
Sbjct: 99 MSGRRSRGSVSEEEINELISRLQTLLP 179
>gb|BG262604.1|BG262604 WHE0938_B02_C04ZS Wheat 5-15 DAP spike cDNA library Triticum
aestivum cDNA clone WHE0938_B02_C04, mRNA sequence
Length = 702
Score = 86.8 bits (183), Expect(2) = 6e-023
Identities = 35/46 (76%), Positives = 43/46 (93%)
Frame = +2 / +1
Query: 197 KLLKETCAYVKSLHREVDDLSERLSGLMATMDNDSPQAEIIRSLLR 334
KLLKETC+Y+KSLHREVDDLS+RLS LM+TMD++S +AEIIR +LR
Sbjct: 316 KLLKETCSYIKSLHREVDDLSDRLSDLMSTMDHNSAEAEIIRGILR 453
Score = 41.4 bits (84), Expect(2) = 6e-023
Identities = 16/21 (76%), Positives = 20/21 (95%)
Frame = +2 / +1
Query: 92 RGRISDDEINELISKLQALLP 154
RG ISD+E+NEL+SKLQ+LLP
Sbjct: 214 RGAISDEEVNELMSKLQSLLP 276
>gb|CA730249.1|CA730249 wip1c.pk003.b11 wip1c Triticum aestivum cDNA clone wip1c.pk003.b11
5' end, mRNA sequence
Length = 612
Score = 86.8 bits (183), Expect(2) = 6e-023
Identities = 35/46 (76%), Positives = 43/46 (93%)
Frame = +2 / +3
Query: 197 KLLKETCAYVKSLHREVDDLSERLSGLMATMDNDSPQAEIIRSLLR 334
KLLKETC+Y+KSLHREVDDLS+RLS LM+TMD++S +AEIIR +LR
Sbjct: 339 KLLKETCSYIKSLHREVDDLSDRLSDLMSTMDHNSAEAEIIRGILR 476
Score = 41.4 bits (84), Expect(2) = 6e-023
Identities = 16/21 (76%), Positives = 20/21 (95%)
Frame = +2 / +3
Query: 92 RGRISDDEINELISKLQALLP 154
RG ISD+E+NEL+SKLQ+LLP
Sbjct: 237 RGSISDEEVNELMSKLQSLLP 299
>gb|BJ212167.1|BJ212167 BJ212167 Y. Ogihara unpublished cDNA library, Wh Triticum aestivum
cDNA clone wh35b21 5', mRNA sequence
Length = 549
Score = 86.8 bits (183), Expect(2) = 6e-023
Identities = 35/46 (76%), Positives = 43/46 (93%)
Frame = +2 / +3
Query: 197 KLLKETCAYVKSLHREVDDLSERLSGLMATMDNDSPQAEIIRSLLR 334
KLLKETC+Y+KSLHREVDDLS+RLS LM+TMD++S +AEIIR +LR
Sbjct: 333 KLLKETCSYIKSLHREVDDLSDRLSDLMSTMDHNSAEAEIIRGILR 470
Score = 41.4 bits (84), Expect(2) = 6e-023
Identities = 16/21 (76%), Positives = 20/21 (95%)
Frame = +2 / +3
Query: 92 RGRISDDEINELISKLQALLP 154
RG ISD+E+NEL+SKLQ+LLP
Sbjct: 231 RGAISDEEVNELMSKLQSLLP 293
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 647,408,000
Number of Sequences: 636343
Number of extensions: 8698900
Number of successful extensions: 141941
Number of sequences better than 1.0e-020: 9
length of database: 122,413,413
effective HSP length: 53
effective length of database: 88,687,234
effective search space used: 17205323396
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)