TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3713493.2.1
(696 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA627122.1|CA627122 wl1n.pk152.h1 wl1n Triticum aestivum... 293 1e-078
gb|CK161226.1|CK161226 FGAS013791 Triticum aestivum FGAS: L... 133 3e-030
gb|CD934031.1|CD934031 GR45.122M12R010831 GR45 Triticum aes... 131 7e-030
gb|CA706232.1|CA706232 wdk1c.pk023.m21 wdk1c Triticum aesti... 131 1e-029
gb|BQ237161.1|BQ237161 TaE05021A03F TaE05 Triticum aestivum... 129 4e-029
gb|CA665939.1|CA665939 wlk1.pk0023.h8 wlk1 Triticum aestivu... 94 1e-023
gb|CD896602.1|CD896602 G174.103F20F010822 G174 Triticum aes... 94 1e-022
>gb|CA627122.1|CA627122 wl1n.pk152.h1 wl1n Triticum aestivum cDNA clone wl1n.pk152.h1 5'
end, mRNA sequence
Length = 495
Score = 293 bits (635), Expect = 1e-078
Identities = 119/121 (98%), Positives = 119/121 (98%)
Frame = +3 / -1
Query: 330 AWFSRGNYSYGRQEAWRFRISWAHGPDLLPTAVDSSNSISLLHGCCSTVGRPGHGNGARD 509
AWFSRG YSYGRQEAWRFRISWAHGP LLPTAVDSSNSISLLHGCCSTVGRPGHGNGARD
Sbjct: 462 AWFSRGXYSYGRQEAWRFRISWAHGPVLLPTAVDSSNSISLLHGCCSTVGRPGHGNGARD 283
Query: 510 VVRAVAPGDGSERVAGIHDHRVA*HCDGDFVSQALLLPVERELARGHRDIHAVRAGDRRL 689
VVRAVAPGDGSERVAGIHDHRVA*HCDGDFVSQALLLPVERELARGHRDIHAVRAGDRRL
Sbjct: 282 VVRAVAPGDGSERVAGIHDHRVA*HCDGDFVSQALLLPVERELARGHRDIHAVRAGDRRL 103
Query: 690 V 692
V
Sbjct: 102 V 100
Score = 279 bits (603), Expect = 3e-074
Identities = 119/123 (96%), Positives = 120/123 (97%)
Frame = +1 / -2
Query: 325 LLRGSVEEIIPMAGRRRGDFGSHGLMALTFFPQR*TVATASVYYMAAAPPLDGQVTAMGL 504
LLRGSVEE I MAGRRRGDF SHGLMAL+FFPQR*TVATASVYYMAAAPPLDGQVTAMGL
Sbjct: 467 LLRGSVEEXIXMAGRRRGDFESHGLMALSFFPQR*TVATASVYYMAAAPPLDGQVTAMGL 288
Query: 505 VTSCAPSLQVMDPNE*LESTITGLPDTAMVISYPRLCCFPSNASLLGVTVTSTPYGLAIV 684
VTSCAPSLQVMDPNE*LESTITGLPDTAMVISYPRLCCFPSNASLLGVTVTSTPYGLAIV
Sbjct: 287 VTSCAPSLQVMDPNE*LESTITGLPDTAMVISYPRLCCFPSNASLLGVTVTSTPYGLAIV 108
Query: 685 GWY 693
GWY
Sbjct: 107 GWY 99
Score = 248 bits (536), Expect = 5e-065
Identities = 107/123 (86%), Positives = 107/123 (86%)
Frame = -2 / +1
Query: 692 YQPTIASPYGVDVTVTPSKLAFDGKQQSLGYEITIAVSGNPVIVDSSYSFGSITWSDGAH 513
YQPTIASPYGVDVTVTPSKLAFDGKQQSLGYEITIAVSGNPVIVDSSYSFGSITWSDGAH
Sbjct: 100 YQPTIASPYGVDVTVTPSKLAFDGKQQSLGYEITIAVSGNPVIVDSSYSFGSITWSDGAH 279
Query: 512 DVTSPIAVTWPSNGGXXXXXXXXXXXXVYRCGKKVRAMSP*DPKSPRLLPAIGIISSTEP 333
DVTSPIAVTWPSNGG VYRCGKK RAMSP*D KSPRLLPAI I SSTEP
Sbjct: 280 DVTSPIAVTWPSNGGAAAM**TDAVATVYRCGKKDRAMSP*DSKSPRLLPAIXIXSSTEP 459
Query: 332 RNN 324
RNN
Sbjct: 460 RNN 468
Score = 248 bits (535), Expect = 7e-065
Identities = 107/126 (84%), Positives = 108/126 (85%)
Frame = -3 / +2
Query: 691 TSRRSPARTAWMXXXXXXXXXXXXXXRAWDTKSPSQCQATR*SWIPATRSDPSPGATART 512
TSRRSPARTAWM RAWDTKSPSQCQATR*SWIPATRSDPSPGATART
Sbjct: 101 TSRRSPARTAWMSR*PRASSRSTGSSRAWDTKSPSQCQATR*SWIPATRSDPSPGATART 280
Query: 511 TSRAPLP*PGRPTVEQQPCSRLMLLLLSTAVGRRSGP*AHEIRNLHASCLP*E*FPRLNH 332
TSRAPLP*PGRPTVEQQPCSRLMLLLLSTAVGRR+GP*AHEIRNLHASCLP E* PRLNH
Sbjct: 281 TSRAPLP*PGRPTVEQQPCSRLMLLLLSTAVGRRTGP*AHEIRNLHASCLPXE*XPRLNH 460
Query: 331 AIIQLP 314
AII P
Sbjct: 461 AIINCP 478
Score = 138 bits (296), Expect(2) = 9e-065
Identities = 53/60 (88%), Positives = 55/60 (91%)
Frame = -1 / +3
Query: 489 DLAVQRWSSSHVVD*CCCYCLPLWEEGQGHEPMRSEISTPPACHRNNFLD*TTQ*FSCPL 310
DLAVQRWSSSHVVD*CCCYCLPLWEEGQGHEPMR EISTPPACH N FLD*TTQ* + P+
Sbjct: 303 DLAVQRWSSSHVVD*CCCYCLPLWEEGQGHEPMRFEISTPPACHXNXFLD*TTQ*STAPI 482
Score = 129 bits (277), Expect(2) = 9e-065
Identities = 51/51 (100%), Positives = 51/51 (100%)
Frame = -1 / +3
Query: 696 GVPADDRQPVRRGCHGDPEQARVRREAAEPGIRNHHRSVRQPGDRGFQLLV 544
GVPADDRQPVRRGCHGDPEQARVRREAAEPGIRNHHRSVRQPGDRGFQLLV
Sbjct: 96 GVPADDRQPVRRGCHGDPEQARVRREAAEPGIRNHHRSVRQPGDRGFQLLV 248
Score = 116 bits (248), Expect(2) = 3e-047
Identities = 46/46 (100%), Positives = 46/46 (100%)
Frame = +2 / -3
Query: 557 NPRSPGCLTLRW*FRIPGSAASRRTRACSGSP*HPRRTGWRSSAGT 694
NPRSPGCLTLRW*FRIPGSAASRRTRACSGSP*HPRRTGWRSSAGT
Sbjct: 235 NPRSPGCLTLRW*FRIPGSAASRRTRACSGSP*HPRRTGWRSSAGT 98
Score = 93.2 bits (197), Expect(2) = 3e-047
Identities = 37/57 (64%), Positives = 40/57 (70%)
Frame = +2 / -3
Query: 314 GQLNYCVVQSRKLFLWQAGGVEISDLMGSWP*PXXXXXXXXXXXXXTTWLLLHRWTA 484
G ++YCVVQSR LF WQAGGVEIS+LMGSWP P TTWLLLHRWTA
Sbjct: 478 GAVDYCVVQSRXLFXWQAGGVEISNLMGSWPCPSSHSGRQ*QQHQSTTWLLLHRWTA 308
>gb|CK161226.1|CK161226 FGAS013791 Triticum aestivum FGAS: Library 4 Gate 8 Triticum
aestivum cDNA, mRNA sequence
Length = 1139
Score = 133 bits (284), Expect = 3e-030
Identities = 51/75 (68%), Positives = 61/75 (81%)
Frame = -2 / +1
Query: 692 YQPTIASPYGVDVTVTPSKLAFDGKQQSLGYEITIAVSGNPVIVDSSYSFGSITWSDGAH 513
Y+ + SP GVD VTP+KL FD + +SL YEIT+AVSGNPVIVD+ YSFGS+TWSDG H
Sbjct: 370 YEAKVESPAGVDAKVTPAKLVFDEEHRSLAYEITLAVSGNPVIVDAKYSFGSVTWSDGKH 549
Query: 512 DVTSPIAVTWPSNGG 468
+VTSPIAVTWP + G
Sbjct: 550 NVTSPIAVTWPESAG 594
Score = 121 bits (259), Expect = 8e-027
Identities = 57/85 (67%), Positives = 60/85 (70%)
Frame = +1 / -1
Query: 424 R*TVATASVYYMAAAPPLDGQVTAMGLVTSCAPSLQVMDPNE*LESTITGLPDTAMVISY 603
R T A+ YM AAP L G VTAMGLVT C PSL V DPNE L STITGLPDTA VISY
Sbjct: 638 RVTPASLQHLYMDAAPALSGHVTAMGLVTLCLPSLHVTDPNEYLASTITGLPDTARVISY 459
Query: 604 PRLCCFPSNASLLGVTVTSTPYGLA 678
RL C SN SL GVT+ STP GL+
Sbjct: 458 ARLRCSSSNTSLAGVTLASTPAGLS 384
>gb|CD934031.1|CD934031 GR45.122M12R010831 GR45 Triticum aestivum cDNA clone GR45122M12,
mRNA sequence
Length = 579
Score = 131 bits (281), Expect = 7e-030
Identities = 51/75 (68%), Positives = 61/75 (81%)
Frame = -2 / -2
Query: 692 YQPTIASPYGVDVTVTPSKLAFDGKQQSLGYEITIAVSGNPVIVDSSYSFGSITWSDGAH 513
Y+ + SP GVD VTP+KL FD + +SL YEIT+AVSGNPVIVD+ YSFGS+TWSDG H
Sbjct: 365 YEA*VESPAGVDAKVTPAKLVFDEEHRSLAYEITLAVSGNPVIVDAKYSFGSVTWSDGKH 186
Query: 512 DVTSPIAVTWPSNGG 468
+VTSPIAVTWP + G
Sbjct: 185 NVTSPIAVTWPESAG 141
Score = 121 bits (259), Expect = 8e-027
Identities = 57/85 (67%), Positives = 60/85 (70%)
Frame = +1 / +1
Query: 424 R*TVATASVYYMAAAPPLDGQVTAMGLVTSCAPSLQVMDPNE*LESTITGLPDTAMVISY 603
R T A+ YM AAP L G VTAMGLVT C PSL V DPNE L STITGLPDTA VISY
Sbjct: 97 RVTPASLQHLYMDAAPALSGHVTAMGLVTLCLPSLHVTDPNEYLASTITGLPDTARVISY 276
Query: 604 PRLCCFPSNASLLGVTVTSTPYGLA 678
RL C SN SL GVT+ STP GL+
Sbjct: 277 ARLRCSSSNTSLAGVTLASTPAGLS 351
>gb|CA706232.1|CA706232 wdk1c.pk023.m21 wdk1c Triticum aestivum cDNA clone wdk1c.pk023.m21
5' end, mRNA sequence
Length = 417
Score = 131 bits (280), Expect = 1e-029
Identities = 50/75 (66%), Positives = 58/75 (77%)
Frame = -2 / -1
Query: 692 YQPTIASPYGVDVTVTPSKLAFDGKQQSLGYEITIAVSGNPVIVDSSYSFGSITWSDGAH 513
Y+ + SP GVD TVTP KL FD + +SL YEIT+ SGNPVIVD YSFGS+TWSDG H
Sbjct: 255 YEAKVESPAGVDATVTPXKLVFDEEHRSLAYEITLXASGNPVIVDGKYSFGSVTWSDGKH 76
Query: 512 DVTSPIAVTWPSNGG 468
+VTSPIAVTWP + G
Sbjct: 75 NVTSPIAVTWPESAG 31
Score = 118 bits (253), Expect = 5e-026
Identities = 54/80 (67%), Positives = 56/80 (70%)
Frame = +1 / +2
Query: 454 YMAAAPPLDGQVTAMGLVTSCAPSLQVMDPNE*LESTITGLPDTAMVISYPRLCCFPSNA 633
YM AAP L G VTAMGLVT C PSL V DPNE L STITGLPD VISY RL C SN
Sbjct: 17 YMDAAPALSGHVTAMGLVTLCLPSLHVTDPNEYLPSTITGLPDAXRVISYARLRCSSSNT 196
Query: 634 SLLGVTVTSTPYGLAIVGWY 693
SL GVTV STP GL+ + Y
Sbjct: 197 SLXGVTVASTPAGLSTLASY 256
>gb|BQ237161.1|BQ237161 TaE05021A03F TaE05 Triticum aestivum cDNA clone TaE05021A03F, mRNA
sequence
Length = 275
Score = 129 bits (276), Expect = 4e-029
Identities = 50/71 (70%), Positives = 59/71 (83%)
Frame = -2 / -1
Query: 680 IASPYGVDVTVTPSKLAFDGKQQSLGYEITIAVSGNPVIVDSSYSFGSITWSDGAHDVTS 501
+ SP GVD VTP+KL FD + +SL YEIT+AVSGNPVIVD+ YSFGS+TWSDG H+VTS
Sbjct: 266 VDSPAGVDAKVTPAKLVFDQEHRSLAYEITLAVSGNPVIVDAKYSFGSVTWSDGKHNVTS 87
Query: 500 PIAVTWPSNGG 468
PIAVTWP + G
Sbjct: 86 PIAVTWPESAG 54
Score = 118 bits (252), Expect = 7e-026
Identities = 54/75 (72%), Positives = 56/75 (74%)
Frame = +1 / +1
Query: 454 YMAAAPPLDGQVTAMGLVTSCAPSLQVMDPNE*LESTITGLPDTAMVISYPRLCCFPSNA 633
YM AAP L G VTAMGLVT C PSL V DPNE L STITGLPDTA VISY RL C SN
Sbjct: 40 YMDAAPALSGHVTAMGLVTLCLPSLHVTDPNEYLASTITGLPDTARVISYARLRCSWSNT 219
Query: 634 SLLGVTVTSTPYGLA 678
SL GVT+ STP GL+
Sbjct: 220 SLAGVTLASTPAGLS 264
Score = 102 bits (217), Expect = 5e-021
Identities = 45/76 (59%), Positives = 53/76 (69%)
Frame = +3 / +3
Query: 453 LHGCCSTVGRPGHGNGARDVVRAVAPGDGSERVAGIHDHRVA*HCDGDFVSQALLLPVER 632
+HG S RP HG+GARD+V AVAP DG ERV G+HDHRVA H +GD V QA +L VE
Sbjct: 39 IHGRRSGALRPRHGDGARDIVLAVAPRDGPERVLGVHDHRVAGHGEGDLVRQAPVLLVEH 218
Query: 633 ELARGHRDIHAVRAGD 680
+L R H +HA RA D
Sbjct: 219 QLGRRHLGVHAGRAVD 266
>gb|CA665939.1|CA665939 wlk1.pk0023.h8 wlk1 Triticum aestivum cDNA clone wlk1.pk0023.h8 5'
end, mRNA sequence
Length = 513
Score = 93.6 bits (198), Expect(2) = 1e-023
Identities = 35/44 (79%), Positives = 40/44 (90%)
Frame = -2 / -1
Query: 599 EITIAVSGNPVIVDSSYSFGSITWSDGAHDVTSPIAVTWPSNGG 468
EIT+AVSGNPVIVD+ YSFGS+TWSDG H+VTSPIAVTWP + G
Sbjct: 216 EITLAVSGNPVIVDAKYSFGSVTWSDGKHNVTSPIAVTWPESAG 85
Score = 36.8 bits (74), Expect(2) = 1e-023
Identities = 15/32 (46%), Positives = 20/32 (62%)
Frame = -2 / -3
Query: 695 GYQPTIASPYGVDVTVTPSKLAFDGKQQSLGY 600
G + + S GVD VTP+KL FD + +SL Y
Sbjct: 313 GLRAKVESXAGVDAKVTPAKLVFDEEHRSLAY 218
>gb|CD896602.1|CD896602 G174.103F20F010822 G174 Triticum aestivum cDNA clone G174103F20,
mRNA sequence
Length = 618
Score = 94.1 bits (199), Expect(2) = 1e-022
Identities = 36/55 (65%), Positives = 43/55 (78%)
Frame = -2 / +3
Query: 692 YQPTIASPYGVDVTVTPSKLAFDGKQQSLGYEITIAVSGNPVIVDSSYSFGSITW 528
Y+ + SP GVD TVTP+KL FD + +SL YEIT+A SGNPVIVD YSFGS+TW
Sbjct: 387 YEAKVESPAGVDATVTPAKLVFDEEHRSLAYEITLAASGNPVIVDGKYSFGSVTW 551
Score = 33.1 bits (66), Expect(2) = 1e-022
Identities = 13/20 (65%), Positives = 15/20 (75%)
Frame = -2 / +1
Query: 527 SDGAHDVTSPIAVTWPSNGG 468
SDG H+VTSPIA T P + G
Sbjct: 553 SDGKHNVTSPIAGTGPESAG 612
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 836,338,946
Number of Sequences: 636343
Number of extensions: 15448088
Number of successful extensions: 189300
Number of sequences better than 1.0e-020: 11
length of database: 122,413,413
effective HSP length: 53
effective length of database: 88,687,234
effective search space used: 15786327652
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)