TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3665525.2.1
(620 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CV066458.1|CV066458 WNEL4b5 Wheat EST endosperm library ... 281 7e-075
gb|BF473884.1|BF473884 WHE0932_D09_H18ZS Wheat 5-15 DAP spi... 270 1e-071
gb|CA657314.1|CA657314 wlm0.pk0034.f11 wlm0 Triticum aestiv... 71 6e-026
>gb|CV066458.1|CV066458 WNEL4b5 Wheat EST endosperm library Triticum aestivum cDNA clone
WNEL4b5 5' similar to Oryza sativa, mRNA sequence
Length = 546
Score = 281 bits (607), Expect = 7e-075
Identities = 110/132 (83%), Positives = 114/132 (86%)
Frame = -2 / +2
Query: 619 IGCMKKGPVFTDPKLKWYEPQSFLLGSEYFLHAYGPIYXXXXXXXXXXXXLRNNSFRMFS 440
IGCMKKGPVFTDPKLKWYEPQSFLLGSEYFLHAYGPIY LRNNSFRMF+
Sbjct: 5 IGCMKKGPVFTDPKLKWYEPQSFLLGSEYFLHAYGPIYALSADVVASLVALRNNSFRMFN 184
Query: 439 I*DVTIGSWMLAMNVNHENTHALCEPDCTESSVAVWDIPKCSGLCHPEVKMLELHQRKEC 260
DVTIGSWMLAMNVNHENTHALCEP+CT SS+AVWDIPKCSGLCHPEVKMLELHQRKEC
Sbjct: 185 NEDVTIGSWMLAMNVNHENTHALCEPECTASSIAVWDIPKCSGLCHPEVKMLELHQRKEC 364
Query: 259 TGGPTVAAEVSE 224
TGGPT AAE +
Sbjct: 365 TGGPTEAAETDD 400
Score = 221 bits (477), Expect = 6e-057
Identities = 93/132 (70%), Positives = 98/132 (74%)
Frame = +3 / -1
Query: 225 SDTSAATVGPPVHSFLXXXXXIFTSGWHSPEHFGMSQTATEDSVQSGSQSACVFSWLTFI 404
S SAA+VGPPVHSF IFTSGWHSPEHFG+SQTA ED+V SGS ACVFSWLTFI
Sbjct: 399 SSVSAASVGPPVHSFRW*SSSIFTSGWHSPEHFGISQTAIEDAVHSGSHRACVFSWLTFI 220
Query: 405 ASIHDPIVTS*ILNIRXXXXXXXXXXXXXXXXXXXIGPYA*RKYSDPSKKDWGSYHFNLG 584
ASI DPIVTS +LNIR IGPYA*RKYSDPS+KD GSYHFNLG
Sbjct: 219 ASIQDPIVTSSLLNIRKLLFLRATRLATTSADKA*IGPYA*RKYSDPSRKDCGSYHFNLG 40
Query: 585 SVKTGPFFMHPM 620
SVKTGPFFMHPM
Sbjct: 39 SVKTGPFFMHPM 4
Score = 200 bits (431), Expect = 1e-050
Identities = 84/125 (67%), Positives = 96/125 (76%)
Frame = -1 / +1
Query: 620 HWMHEEGACFY*S*VEMV*APILLTRIRILSSCIWANLCFIS*RGG*LGCFEEQQFPYV* 441
HWMHEEGACFY*S +EMV A +L T IRIL SCIWA LCFIS* GG GC E+QQF V
Sbjct: 4 HWMHEEGACFY*SQIEMVRATVLPTWIRILPSCIWAYLCFIS*CGGKPGCSEKQQFSNVQ 183
Query: 440 YLGRYNWIVDACYERQP*EHTCAL*T*LHGVFSCCLGHSKMLRAMPPRGKNARASSKKRM 261
G Y WI+DACYERQP EH C++ T +H V +CCLG+SKMLRAMPP GK+A AS +R+
Sbjct: 184 Q*GCYYWILDACYERQPREHACSVRTRVHSVLNCCLGYSKMLRAMPPGGKDAGASPAERV 363
Query: 260 HRWSN 246
H WSN
Sbjct: 364 HGWSN 378
Score = 196 bits (423), Expect = 2e-049
Identities = 87/124 (70%), Positives = 101/124 (81%)
Frame = +2 / -3
Query: 248 WTTCAFFSLMKL*HFYLWVA*P*AFWNVPNSN*RLRAVRFTERMCVLMVDVHSKHPRSNC 427
WTT A F L+KL H YL VA*P*AFWN+P+SN* A+ F + M VL+VDVHSKHP SN
Sbjct: 376 WTTRALFPLVKLQHLYLRVA*P*AFWNIPDSN*GRCALGFAQSMRVLVVDVHSKHPGSNS 197
Query: 428 NVLDTKHTETVVPQSNQASHHVS**SINWPICMKKVF*SE*EGLGLIPFQLRISKNRPLL 607
N+L +H++TVV QSNQA HH+S**SIN PICMK+VF*S+*EGL L+PFQ ISKNRPLL
Sbjct: 196 NILIVEHSKTVVSQSNQACHHIS**SINRPICMKEVF*SK*EGLWLVPFQFGISKNRPLL 17
Query: 608 HASN 619
HASN
Sbjct: 16 HASN 5
Score = 177 bits (380), Expect = 1e-043
Identities = 84/124 (67%), Positives = 91/124 (73%)
Frame = +1 / -2
Query: 247 LDHLCILFFDEALAFLPLGGIALSILECPKQQLKTPCSQVHRAHVCSHG*RS*QASTIQL 426
LDH C L EA A LP GGIALSILE P+QQL+T C++V H CS G*RS*QAS IQ
Sbjct: 377 LDHPCTLSAGEAPASLPPGGIALSILEYPRQQLRTLCTRVRTEHACSRG*RS*QASRIQ* 198
Query: 427 *RPRY*TYGNCCSSKQPS*PPRQLIKHKLAHMHEESILIRVRRIGAHTIST*DQ*KQAPS 606
* P *T+ NCC S+QP PP QLIKHK AHMHE SILI+V R A TIS DQ*KQAPS
Sbjct: 197 *HPHC*TFENCCFSEQPGLPPHQLIKHK*AHMHEGSILIQVGRTVARTISIWDQ*KQAPS 18
Query: 607 SCIQ 618
SCIQ
Sbjct: 17 SCIQ 6
Score = 86.8 bits (183), Expect(2) = 6e-033
Identities = 41/67 (61%), Positives = 47/67 (70%)
Frame = -3 / +3
Query: 447 CLVSRTLQLDRGCLL*TSTMRTHMRSVNLTARSLQLLFGTFQNAQGYATQR*KC*SFIKE 268
C R L LD GCLL*TST RT M N +A+ QLL G FQNAQGYAT+R*+C SF
Sbjct: 177 CSTMRMLLLDPGCLL*TSTTRTRMLCANPSAQRPQLLSGIFQNAQGYATRR*RCWSFTSG 356
Query: 267 KNAQVVQ 247
K+A+VVQ
Sbjct: 357 KSARVVQ 377
Score = 74.8 bits (157), Expect(2) = 6e-033
Identities = 33/41 (80%), Positives = 35/41 (85%)
Frame = -3 / +3
Query: 618 LDA*RRGLFLLILS*NGMSPNPSYSDQNTFFMHMGQFMLYQ 496
LDA*RRGLFLLI +*NG S +PSY DQNT FMHMG FMLYQ
Sbjct: 6 LDA*RRGLFLLIPN*NGTSHSPSYLDQNTSFMHMGLFMLYQ 128
>gb|BF473884.1|BF473884 WHE0932_D09_H18ZS Wheat 5-15 DAP spike cDNA library Triticum
aestivum cDNA clone WHE0932_D09_H18, mRNA sequence
Length = 651
Score = 270 bits (584), Expect = 1e-071
Identities = 105/125 (84%), Positives = 109/125 (87%)
Frame = -2 / +3
Query: 619 IGCMKKGPVFTDPKLKWYEPQSFLLGSEYFLHAYGPIYXXXXXXXXXXXXLRNNSFRMFS 440
IGCMKKGPVFTDPKLKWYEPQSFLLGSEYFLHAYGPIY +RNNSFRMF+
Sbjct: 48 IGCMKKGPVFTDPKLKWYEPQSFLLGSEYFLHAYGPIYALSADVVASLVAVRNNSFRMFN 227
Query: 439 I*DVTIGSWMLAMNVNHENTHALCEPDCTESSVAVWDIPKCSGLCHPEVKMLELHQRKEC 260
DVTIGSWMLAMNVNHENTHAL EP+CT SS+AVWDIPKCSGLCHPEVKMLELHQRKEC
Sbjct: 228 NEDVTIGSWMLAMNVNHENTHALTEPECTASSIAVWDIPKCSGLCHPEVKMLELHQRKEC 407
Query: 259 TGGPT 245
TGGPT
Sbjct: 408 TGGPT 422
Score = 215 bits (465), Expect = 3e-055
Identities = 90/127 (70%), Positives = 95/127 (74%)
Frame = +3 / -3
Query: 240 ATVGPPVHSFLXXXXXIFTSGWHSPEHFGMSQTATEDSVQSGSQSACVFSWLTFIASIHD 419
++VGPPVHSF IFTSGWHSPEHFGMSQTA ED+V SGS ACVFSWLTFIASI D
Sbjct: 427 SSVGPPVHSFRW*SSSIFTSGWHSPEHFGMSQTAIEDAVHSGSVRACVFSWLTFIASIQD 248
Query: 420 PIVTS*ILNIRXXXXXXXXXXXXXXXXXXXIGPYA*RKYSDPSKKDWGSYHFNLGSVKTG 599
PIVTS +LNIR IGPYA*RKYSDPS+KD GSYHFNLGSVKTG
Sbjct: 247 PIVTSSLLNIRKLLFLTATKLATTSADKA*IGPYA*RKYSDPSRKDCGSYHFNLGSVKTG 68
Query: 600 PFFMHPM 620
PFFMHPM
Sbjct: 67 PFFMHPM 47
Score = 200 bits (432), Expect = 9e-051
Identities = 86/125 (68%), Positives = 96/125 (76%)
Frame = -1 / +2
Query: 620 HWMHEEGACFY*S*VEMV*APILLTRIRILSSCIWANLCFIS*RGG*LGCFEEQQFPYV* 441
HWMHEEGACFY*S +EMV A +L T IRIL SCIWA LCFIS* GG LGC E+QQFP V
Sbjct: 47 HWMHEEGACFY*SQIEMVRATVLPTWIRILPSCIWAYLCFIS*CGGKLGCCEKQQFPDVQ 226
Query: 440 YLGRYNWIVDACYERQP*EHTCAL*T*LHGVFSCCLGHSKMLRAMPPRGKNARASSKKRM 261
G Y WI+DA YERQP EH + T +H V +CCLGHSKMLRAMPP GK+ARAS +R+
Sbjct: 227 QRGCYYWILDARYERQPREHARSHRTRVHSVLNCCLGHSKMLRAMPPGGKDARASPAERV 406
Query: 260 HRWSN 246
H WSN
Sbjct: 407 HGWSN 421
Score = 194 bits (419), Expect = 6e-049
Identities = 88/124 (70%), Positives = 100/124 (80%)
Frame = +2 / -2
Query: 248 WTTCAFFSLMKL*HFYLWVA*P*AFWNVPNSN*RLRAVRFTERMCVLMVDVHSKHPRSNC 427
WTT A F L+KL*H YL VA*P*AFWNVP+SN* A+ F E + VL+VDVHS+HP SN
Sbjct: 419 WTTRALFPLVKL*HLYLRVA*P*AFWNVPDSN*GRCALGFGESVRVLVVDVHSEHPGSNS 240
Query: 428 NVLDTKHTETVVPQSNQASHHVS**SINWPICMKKVF*SE*EGLGLIPFQLRISKNRPLL 607
N+L +H ETVV SNQA HH+S**SIN PICMK+VF*S+*EGL L+PFQ ISKNRPLL
Sbjct: 239 NILVVEHPETVVSHSNQACHHIS**SINRPICMKEVF*SK*EGLWLVPFQFGISKNRPLL 60
Query: 608 HASN 619
HASN
Sbjct: 59 HASN 48
Score = 183 bits (395), Expect = 1e-045
Identities = 87/124 (70%), Positives = 94/124 (75%)
Frame = +1 / -1
Query: 247 LDHLCILFFDEALAFLPLGGIALSILECPKQQLKTPCSQVHRAHVCSHG*RS*QASTIQL 426
LDH C L EALA LP GGIALSILECP+QQL+T C++V CS G*RS*+AS IQ
Sbjct: 420 LDHPCTLSAGEALASLPPGGIALSILECPRQQLRTLCTRVR*ERACSRG*RS*RASRIQ* 241
Query: 427 *RPRY*TYGNCCSSKQPS*PPRQLIKHKLAHMHEESILIRVRRIGAHTIST*DQ*KQAPS 606
* PR *T GNCC S+QPS PP QLIKHK AHMHE SILI+V R A TIS DQ*KQAPS
Sbjct: 240 *HPRC*TSGNCCFSQQPSLPPHQLIKHK*AHMHEGSILIQVGRTVARTISIWDQ*KQAPS 61
Query: 607 SCIQ 618
SCIQ
Sbjct: 60 SCIQ 49
Score = 90.9 bits (192), Expect(2) = 3e-034
Identities = 44/70 (62%), Positives = 51/70 (72%)
Frame = -3 / +1
Query: 456 VSVCLVSRTLQLDRGCLL*TSTMRTHMRSVNLTARSLQLLFGTFQNAQGYATQR*KC*SF 277
VS C +R L LD GC L*TST RT S N +A+ QLL GTFQNAQGYAT+R*+C*SF
Sbjct: 211 VSGCSTTRMLLLDPGCSL*TSTTRTRTLSPNPSAQRPQLLSGTFQNAQGYATRR*RC*SF 390
Query: 276 IKEKNAQVVQ 247
K+A+VVQ
Sbjct: 391 TSGKSARVVQ 420
Score = 74.8 bits (157), Expect(2) = 3e-034
Identities = 33/41 (80%), Positives = 35/41 (85%)
Frame = -3 / +1
Query: 618 LDA*RRGLFLLILS*NGMSPNPSYSDQNTFFMHMGQFMLYQ 496
LDA*RRGLFLLI +*NG S +PSY DQNT FMHMG FMLYQ
Sbjct: 49 LDA*RRGLFLLIPN*NGTSHSPSYLDQNTSFMHMGLFMLYQ 171
>gb|CA657314.1|CA657314 wlm0.pk0034.f11 wlm0 Triticum aestivum cDNA clone wlm0.pk0034.f11
5' end, mRNA sequence
Length = 463
Score = 70.7 bits (148), Expect(3) = 6e-026
Identities = 26/26 (100%), Positives = 26/26 (100%)
Frame = -2 / +1
Query: 619 IGCMKKGPVFTDPKLKWYEPQSFLLG 542
IGCMKKGPVFTDPKLKWYEPQSFLLG
Sbjct: 139 IGCMKKGPVFTDPKLKWYEPQSFLLG 216
Score = 65.7 bits (137), Expect(4) = 1e-023
Identities = 24/26 (92%), Positives = 25/26 (96%)
Frame = +3 / -3
Query: 543 PSKKDWGSYHFNLGSVKTGPFFMHPM 620
PS+KD GSYHFNLGSVKTGPFFMHPM
Sbjct: 215 PSRKDCGSYHFNLGSVKTGPFFMHPM 138
Score = 52.4 bits (108), Expect(3) = 6e-026
Identities = 22/35 (62%), Positives = 27/35 (77%)
Frame = -2 / +3
Query: 466 RNNSFRMFSI*DVTIGSWMLAMNVNHENTHALCEP 362
RNN F+ ++ + +GS MLAMNVNHENTHAL EP
Sbjct: 297 RNNIFQCSTMRMLLLGSGMLAMNVNHENTHALXEP 401
Score = 41.4 bits (84), Expect(4) = 1e-023
Identities = 16/20 (80%), Positives = 17/20 (85%)
Frame = +3 / -1
Query: 363 GSQSACVFSWLTFIASIHDP 422
GS ACVFSWLTFIASI +P
Sbjct: 400 GSXRACVFSWLTFIASIPEP 341
Score = 32.7 bits (65), Expect(3) = 6e-026
Identities = 12/15 (80%), Positives = 13/15 (86%)
Frame = -3 / +1
Query: 540 QNTFFMHMGQFMLYQ 496
+NT FMHMG FMLYQ
Sbjct: 220 KNTSFMHMGLFMLYQ 264
Score = 31.3 bits (62), Expect(4) = 1e-023
Identities = 13/15 (86%), Positives = 13/15 (86%)
Frame = +1 / -2
Query: 496 LIKHKLAHMHEESIL 540
LIKHK AHMHE SIL
Sbjct: 264 LIKHK*AHMHEGSIL 220
Score = 26.7 bits (52), Expect(4) = 1e-023
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = +2 / -1
Query: 413 PRSNCNVLDTKHTETVVPQSNQASH 487
P N N+L +H + + +SNQA H
Sbjct: 349 PEPNSNILIVEHWKMLFLRSNQACH 275
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 638,370,554
Number of Sequences: 636343
Number of extensions: 9803394
Number of successful extensions: 108656
Number of sequences better than 1.0e-020: 4
length of database: 122,413,413
effective HSP length: 53
effective length of database: 88,687,234
effective search space used: 13569146802
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)