TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3092809.2.1
(652 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA651727.1|CA651727 wre1n.pk179.e5 wre1n Triticum aestiv... 153 2e-036
gb|BJ248602.1|BJ248602 BJ248602 Y. Ogihara unpublished cDNA... 82 2e-028
gb|CD871730.1|CD871730 AZO2.118O23F010213 AZO2 Triticum aes... 66 1e-022
gb|BQ606611.1|BQ606611 BRY_2476 wheat EST endosperm library... 81 5e-022
gb|CA742061.1|CA742061 wet1c.pk001.j21 wet1c Triticum aesti... 61 4e-021
>gb|CA651727.1|CA651727 wre1n.pk179.e5 wre1n Triticum aestivum cDNA clone wre1n.pk179.e5 5'
end, mRNA sequence
Length = 445
Score = 153 bits (329), Expect = 2e-036
Identities = 63/80 (78%), Positives = 74/80 (92%)
Frame = +2 / +3
Query: 170 ALSMPHLPQGARADDALKDFLAFVGGEANLKGVDAMPEDVAQAVLYLASDEARYVSAVNL 349
ALSMPHLPQG + DA++DFLAFVG EANLKGVD +P+DVA+AVLYLASDEARY+SA+NL
Sbjct: 3 ALSMPHLPQGEQKGDAVRDFLAFVGSEANLKGVDLLPKDVAEAVLYLASDEARYISALNL 182
Query: 350 MVDGGFTAVNNNLRAFED*L 409
+VDGGFT+VN NL+AFED*L
Sbjct: 183 VVDGGFTSVNGNLKAFED*L 242
>gb|BJ248602.1|BJ248602 BJ248602 Y. Ogihara unpublished cDNA library, Wh_f Triticum
aestivum cDNA clone whf7c11 5', mRNA sequence
Length = 609
Score = 82.2 bits (173), Expect(3) = 2e-028
Identities = 30/42 (71%), Positives = 36/42 (85%)
Frame = +2 / +2
Query: 50 GPHVYTASKHAVVGLTKSVAAELGRHGVRVNCVSPYAVPTAL 175
GPH YTASKHA+VGLTK+ A ELG HG+RVNCVSP+ V T++
Sbjct: 26 GPHAYTASKHALVGLTKNAACELGEHGIRVNCVSPFGVATSM 151
Score = 57.9 bits (120), Expect(3) = 2e-028
Identities = 25/48 (52%), Positives = 32/48 (66%)
Frame = +2 / +2
Query: 239 VGGEANLKGVDAMPEDVAQAVLYLASDEARYVSAVNLMVDGGFTAVNN 382
V G A LKG D+A+A L+LAS+E+RY+S NL+VDGG T N
Sbjct: 254 VRGMATLKGPTLRAGDIAEAALFLASEESRYISGHNLVVDGGVTTSRN 397
Score = 24.0 bits (46), Expect(3) = 2e-028
Identities = 9/16 (56%), Positives = 10/16 (62%)
Frame = +1 / +1
Query: 25 RGQRHRRDGPARLHGV 72
R RDGPA +HGV
Sbjct: 1 RNSARGRDGPACVHGV 48
>gb|CD871730.1|CD871730 AZO2.118O23F010213 AZO2 Triticum aestivum cDNA clone AZO2118O23,
mRNA sequence
Length = 721
Score = 66.1 bits (138), Expect(2) = 1e-022
Identities = 27/64 (42%), Positives = 40/64 (62%)
Frame = +2 / -3
Query: 56 HVYTASKHAVVGLTKSVAAELGRHGVRVNCVSPYAVPTALSMPHLPQGARADDALKDFLA 235
H YT +K A+VGLT++ A ELG+HG+RVNC+SP A T L+ ++ A + + A
Sbjct: 311 HAYTCAKRALVGLTENAATELGQHGIRVNCISPAAAATPLATGYVGLDGEAFEMAMESFA 132
Query: 236 FVGG 247
+ G
Sbjct: 131 NLKG 120
Score = 60.6 bits (126), Expect(2) = 1e-022
Identities = 25/43 (58%), Positives = 32/43 (74%)
Frame = +2 / -3
Query: 251 ANLKGVDAMPEDVAQAVLYLASDEARYVSAVNLMVDGGFTAVN 379
ANLKGV +D+A AVL+LA D+ARYVS NL++DGG + N
Sbjct: 134 ANLKGVGLRVKDIAAAVLFLACDDARYVSGHNLLIDGGISVSN 6
>gb|BQ606611.1|BQ606611 BRY_2476 wheat EST endosperm library Triticum aestivum cDNA 5',
mRNA sequence
Length = 562
Score = 81.3 bits (171), Expect(2) = 5e-022
Identities = 30/42 (71%), Positives = 35/42 (83%)
Frame = +2 / +2
Query: 50 GPHVYTASKHAVVGLTKSVAAELGRHGVRVNCVSPYAVPTAL 175
GPH YTASKHA+VGLTK+ A ELG HG+RVNCVSP+ V T +
Sbjct: 395 GPHAYTASKHALVGLTKNAACELGEHGIRVNCVSPFGVATPM 520
Score = 43.7 bits (89), Expect(2) = 5e-022
Identities = 16/22 (72%), Positives = 19/22 (86%)
Frame = +1 / +1
Query: 7 HRVAGQRGQRHRRDGPARLHGV 72
HRV G+RG+R R DGPAR+HGV
Sbjct: 352 HRVGGERGRRARWDGPARVHGV 417
>gb|CA742061.1|CA742061 wet1c.pk001.j21 wet1c Triticum aestivum cDNA clone wet1c.pk001.j21
5' end, mRNA sequence
Length = 549
Score = 61.1 bits (127), Expect(2) = 4e-021
Identities = 24/38 (63%), Positives = 31/38 (81%)
Frame = +2 / +1
Query: 257 LKGVDAMPEDVAQAVLYLASDEARYVSAVNLMVDGGFT 370
+KG P+D+A+A LYLASDEA+YV+ NL+VDGGFT
Sbjct: 286 MKGAVLEPDDIARAALYLASDEAKYVNGHNLVVDGGFT 399
Score = 60.6 bits (126), Expect(2) = 4e-021
Identities = 25/48 (52%), Positives = 33/48 (68%)
Frame = +2 / +1
Query: 53 PHVYTASKHAVVGLTKSVAAELGRHGVRVNCVSPYAVPTALSMPHLPQ 196
PH Y+ SK AV+G+ ++VA E+ R GVRVN +SP +PT L M L Q
Sbjct: 82 PHPYSVSKTAVLGIVRAVAGEMARSGVRVNAISPNYIPTPLVMRILEQ 225
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 538,153,906
Number of Sequences: 636343
Number of extensions: 7824415
Number of successful extensions: 250454
Number of sequences better than 1.0e-020: 9
length of database: 122,413,413
effective HSP length: 53
effective length of database: 88,687,234
effective search space used: 14456019142
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)