TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2619346.2.2
(1471 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA620365.1|CA620365 wl1n.pk0055.g9 wl1n Triticum aestivu... 281 1e-074
gb|CA620968.1|CA620968 wl1n.pk0070.c12 wl1n Triticum aestiv... 112 5e-030
>gb|CA620365.1|CA620365 wl1n.pk0055.g9 wl1n Triticum aestivum cDNA clone wl1n.pk0055.g9 5'
end, mRNA sequence
Length = 618
Score = 281 bits (608), Expect = 1e-074
Identities = 116/123 (94%), Positives = 117/123 (95%)
Frame = +2 / +3
Query: 8 TIQQF*SKREEDTPQS*QANNLQEQQEWRRRVASLAFSSPCFWQEWPLPPPSTKRLLPAS 187
TIQQF*SKREEDTPQS*QANNLQEQQEWRRRVASLAFSSPCFWQEWPLPPPSTKRLLPAS
Sbjct: 3 TIQQF*SKREEDTPQS*QANNLQEQQEWRRRVASLAFSSPCFWQEWPLPPPSTKRLLPAS 182
Query: 188 GLATARVSRASMDVLPLRCPSRSHNLKLSRSPICSHCPSQNLNRNLCHIQSQSLNLNQSP 367
GLATARVSRASMDVLPLRCPSRSHNLKLSRSPICSHCPSQNLNRNLCHIQSQ +P
Sbjct: 183 GLATARVSRASMDVLPLRCPSRSHNLKLSRSPICSHCPSQNLNRNLCHIQSQKPEPQPNP 362
Query: 368 TLN 376
TLN
Sbjct: 363 TLN 371
Score = 193 bits (416), Expect = 4e-048
Identities = 78/79 (98%), Positives = 78/79 (98%)
Frame = +3 / +1
Query: 6 LRSNSSRARERKTHRRVSRLITCRSSRNGGDASPLLLSPRRASGRSGRCHRLRRSGCCRL 185
LRSNSSRARERKTHRRVSRLITCRSSRNGGDASPLLLSPRRASGRSGRCHRLRRSGCCRL
Sbjct: 1 LRSNSSRARERKTHRRVSRLITCRSSRNGGDASPLLLSPRRASGRSGRCHRLRRSGCCRL 180
Query: 186 RAWPRRAFRAQAWTCCR*D 242
RAWP RAFRAQAWTCCR*D
Sbjct: 181 RAWPXRAFRAQAWTCCR*D 237
Score = 175 bits (376), Expect(3) = 2e-050
Identities = 71/71 (100%), Positives = 71/71 (100%)
Frame = -2 / -3
Query: 300 GQWLHMGLRLSFRLWLRLGHLSGSTSMLARETRAVAKPEAGSSRFVEGGGSGHSCQKHGE 121
GQWLHMGLRLSFRLWLRLGHLSGSTSMLARETRAVAKPEAGSSRFVEGGGSGHSCQKHGE
Sbjct: 295 GQWLHMGLRLSFRLWLRLGHLSGSTSMLARETRAVAKPEAGSSRFVEGGGSGHSCQKHGE 116
Query: 120 EKAREATRRRH 88
EKAREATRRRH
Sbjct: 115 EKAREATRRRH 83
Score = 68.0 bits (142), Expect(3) = 3e-021
Identities = 31/42 (73%), Positives = 31/42 (73%)
Frame = -1 / -2
Query: 133 EARRGESKRGDASPPFXXXXXXXXXXXLRCVFLSLALELLDR 8
EARRGESKRGDASPPF LRCVFLSLALELLDR
Sbjct: 128 EARRGESKRGDASPPFLLLLQVISLLTLRCVFLSLALELLDR 3
Score = 49.2 bits (101), Expect(3) = 3e-021
Identities = 18/18 (100%), Positives = 18/18 (100%)
Frame = -3 / -1
Query: 269 VLGCGSGWGISAAARPCL 216
VLGCGSGWGISAAARPCL
Sbjct: 264 VLGCGSGWGISAAARPCL 211
Score = 42.3 bits (86), Expect(3) = 2e-050
Identities = 21/36 (58%), Positives = 21/36 (58%)
Frame = -3 / -1
Query: 113 QERRRVXXXXXXXXXXXXXXXAVCLPLSCSRTVGS* 6
QERRRV AVCLPLSCSRTVGS*
Sbjct: 108 QERRRVAAIPAAPASY*PANSAVCLPLSCSRTVGS* 1
Score = 24.0 bits (46), Expect(3) = 3e-021
Identities = 9/16 (56%), Positives = 9/16 (56%)
Frame = -2 / -3
Query: 300 GQWLHMGLRLSFRLWL 253
G WL M R FR WL
Sbjct: 343 GFWLWMWHRFRFRFWL 296
Score = 22.6 bits (43), Expect(3) = 2e-050
Identities = 7/10 (70%), Positives = 7/10 (70%)
Frame = -2 / -2
Query: 417 LWFGFWLRLG 388
LW G WL LG
Sbjct: 410 LWXGXWLSLG 381
>gb|CA620968.1|CA620968 wl1n.pk0070.c12 wl1n Triticum aestivum cDNA clone wl1n.pk0070.c12 5'
end, mRNA sequence
Length = 518
Score = 112 bits (240), Expect(4) = 1e-026
Identities = 46/49 (93%), Positives = 46/49 (93%)
Frame = -2 / -1
Query: 1335 KMTKKR*DRYNK*QHLQKHAATWHFI*TLQ*LLIATSSVDSCWWDVWRV 1189
K KKR*DRYNK*QHLQKHAATWHFI*TLQ LLIATSSVDSCWWDVWRV
Sbjct: 428 KNDKKR*DRYNK*QHLQKHAATWHFI*TLQXLLIATSSVDSCWWDVWRV 282
Score = 107 bits (228), Expect(3) = 5e-030
Identities = 45/49 (91%), Positives = 45/49 (91%)
Frame = +3 / +2
Query: 1188 *PSTHPTSSYQLRKWLLTIIVVFK*NAMLQRAFVGAATCCIYPIFSLSF 1334
*PSTHPTSSYQL KWLLT IVVFK*NAMLQRAFVGAATCCIYPIFS F
Sbjct: 281 *PSTHPTSSYQLXKWLLTXIVVFK*NAMLQRAFVGAATCCIYPIFSCHF 427
Score = 101 bits (215), Expect(2) = 1e-021
Identities = 45/50 (90%), Positives = 46/50 (92%)
Frame = -1 / -3
Query: 1336 KNDKEKIG*IQQVAAPTKARCNMAFYLNTTIIVNSHFLS**LLVGCVEGQ 1187
K +EKIG*IQQVAAPTKARCNMAFYLNTTI VNSHF S**LLVGCVEGQ
Sbjct: 429 KK*QEKIG*IQQVAAPTKARCNMAFYLNTTIXVNSHFXS**LLVGCVEGQ 280
Score = 46.0 bits (94), Expect(3) = 5e-030
Identities = 22/39 (56%), Positives = 27/39 (69%)
Frame = +2 / +3
Query: 302 SQNLNRNLCHIQSQSLNLNQSPTLNLNQCPSLNQNPNQS 418
+QN N+N H QSQSL+L Q+ +LN NQ S NQN N S
Sbjct: 114 NQNRNQNRSHNQSQSLSLIQNQSLNPNQNQSPNQNQNLS 230
Score = 26.3 bits (51), Expect(2) = 1e-021
Identities = 8/13 (61%), Positives = 8/13 (61%)
Frame = -2 / -2
Query: 426 RQWLWFGFWLRLG 388
R WLWF FW G
Sbjct: 106 RLWLWFWFWSWFG 68
Score = 24.0 bits (46), Expect(4) = 1e-026
Identities = 8/15 (53%), Positives = 9/15 (60%)
Frame = -2 / -2
Query: 294 WLHMGLRLSFRLWLR 250
WL + LR FR W R
Sbjct: 154 WLWLWLRFWFRFWFR 110
Score = 23.1 bits (44), Expect(4) = 1e-026
Identities = 6/13 (46%), Positives = 6/13 (46%)
Frame = -2 / -2
Query: 426 RQWLWFGFWLRLG 388
R W W G W G
Sbjct: 226 RFWFWLGLWFWFG 188
Score = 22.1 bits (42), Expect(3) = 5e-030
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = +2 / +1
Query: 1334 FCMLCYYMILVQFS 1375
F ++ YYMILVQ +
Sbjct: 424 FFVVSYYMILVQLA 465
Score = 21.7 bits (41), Expect(4) = 1e-026
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = -1 / -3
Query: 1369 LH*DHIIA*HTK 1334
LH*DHIIA + K
Sbjct: 459 LH*DHIIAYYKK 424
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 588,000,621
Number of Sequences: 636343
Number of extensions: 7940812
Number of successful extensions: 112881
Number of sequences better than 1.0e-020: 4
length of database: 122,413,413
effective HSP length: 55
effective length of database: 87,414,548
effective search space used: 37937913832
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)