TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2594342.2.1
(715 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CD490362.1|CD490362 WHE2497_A05_B09ZT Triticum monococcu... 175 4e-043
gb|CK211338.1|CK211338 FGAS023176 Triticum aestivum FGAS: L... 173 2e-042
gb|CK208141.1|CK208141 FGAS019822 Triticum aestivum FGAS: L... 171 8e-042
>gb|CD490362.1|CD490362 WHE2497_A05_B09ZT Triticum monococcum DV92 early reproductive apex
cDNA library Triticum monococcum cDNA clone
WHE2497_A05_B09, mRNA sequence
Length = 705
Score = 175 bits (377), Expect = 4e-043
Identities = 75/105 (71%), Positives = 84/105 (80%)
Frame = -2 / -2
Query: 711 GALCEGLAFNVPLRVRNAGRRSGGPTVFLFSSPPAVPNAPAKPLLGFEKVSLEPGQAGVV 532
G C LAF+V LRV+NAG +G +V LFSSPP+ NAPAK LLGFEKVSL PG+AG V
Sbjct: 581 GDHCGDLAFDVKLRVQNAGEVAGAHSVLLFSSPPSAHNAPAKHLLGFEKVSLAPGEAGTV 402
Query: 531 AFKVDVCKDLSVVDELGNRKVALGSHTLHVGDLKHTLNLGV*WPR 397
AF+VDVC+DLSV DELG RKVALG HTLHVGDLKHT+ L V*W R
Sbjct: 401 AFRVDVCRDLSVADELGGRKVALGGHTLHVGDLKHTVELRV*WSR 267
Score = 110 bits (235), Expect(2) = 1e-023
Identities = 52/105 (49%), Positives = 56/105 (53%)
Frame = +2 / +1
Query: 398 LGHQTPKLSVCLRSPTCSVWXXXXXXXXXXXXXXXXXXXXXXXNATTPACPGSSDTFSKP 577
L HQT +VCLRSPTCSVW NAT PA PG+SDTFS P
Sbjct: 268 LDHQTRSSTVCLRSPTCSVWPPSATLRPPSSSATLRSLHTSTLNATVPASPGASDTFSNP 447
Query: 578 SRGFAGALGTAGGDENRNTVGPPDLLPAFRTRRGTLNARPSQSAP 712
SR FAGAL GGDEN +T P PAF TR T AR Q +P
Sbjct: 448 SRCFAGALCADGGDENSSTECAPATSPAFCTRSFTSKARSPQWSP 582
Score = 73.9 bits (155), Expect(2) = 3e-027
Identities = 31/58 (53%), Positives = 32/58 (55%)
Frame = -3 / -3
Query: 572 SKRCRWSPGRRAWWRSRWTCARTSASWTSWATGRWRWAAXXXXXXXXXXXXXXXSDGR 399
S+RCRW PGR A WRS WTCA T A TSWA RWR AA SDGR
Sbjct: 442 SRRCRWRPGRPARWRSEWTCAGT*AWRTSWAAARWRSAATRCTLATSSTPWSYGSDGR 269
Score = 68.9 bits (144), Expect(2) = 3e-027
Identities = 31/45 (68%), Positives = 35/45 (77%)
Frame = -1 / -1
Query: 715 QGRALRGPGVQRAPPGAERGQKVRRAHGVPVLVAAGGAQRAGEAP 581
+GR LRGPG++R PGAER + RRA G VLVAA GAQRAGEAP
Sbjct: 585 RGRPLRGPGLRREAPGAERRRGGRRALGAAVLVAAVGAQRAGEAP 451
Score = 22.1 bits (42), Expect(2) = 1e-023
Identities = 8/17 (47%), Positives = 11/17 (64%)
Frame = +2 / +3
Query: 218 SNASKQMTKSCPWIQFF 268
+N +K K+ WIQFF
Sbjct: 69 NNRNKTTPKAPRWIQFF 119
>gb|CK211338.1|CK211338 FGAS023176 Triticum aestivum FGAS: Library 6 CAP GATE 1 Triticum
aestivum cDNA, mRNA sequence
Length = 1056
Score = 173 bits (373), Expect = 2e-042
Identities = 75/102 (73%), Positives = 83/102 (81%)
Frame = -2 / -3
Query: 711 GALCEGLAFNVPLRVRNAGRRSGGPTVFLFSSPPAVPNAPAKPLLGFEKVSLEPGQAGVV 532
G CE LAF+V LRVRNAG +G +V LFSSPP+ NAPAK LLGFEKVSL PG+AG V
Sbjct: 595 GDHCEDLAFDVKLRVRNAGEVAGAHSVLLFSSPPSAHNAPAKHLLGFEKVSLAPGEAGTV 416
Query: 531 AFKVDVCKDLSVVDELGNRKVALGSHTLHVGDLKHTLNLGV* 406
AF+VDVC+DLSV DELG RKVALG HTLHVGDLKHT+ L V*
Sbjct: 415 AFRVDVCRDLSVADELGGRKVALGGHTLHVGDLKHTVELRV* 290
Score = 111 bits (237), Expect(2) = 2e-024
Identities = 52/102 (50%), Positives = 56/102 (54%)
Frame = +2 / +3
Query: 407 QTPKLSVCLRSPTCSVWXXXXXXXXXXXXXXXXXXXXXXXNATTPACPGSSDTFSKPSRG 586
QT +VCLRSPTCSVW NAT PA PG+SDTFS PSR
Sbjct: 291 QTRSSTVCLRSPTCSVWPPSATLRPPSSSATLRSLHTSTLNATVPASPGASDTFSNPSRC 470
Query: 587 FAGALGTAGGDENRNTVGPPDLLPAFRTRRGTLNARPSQSAP 712
FAGAL GGDEN +T P PAFRTR T AR SQ +P
Sbjct: 471 FAGALCADGGDENSSTECAPATSPAFRTRSFTSKARSSQWSP 596
Score = 69.8 bits (146), Expect(4) = 1e-029
Identities = 27/39 (69%), Positives = 28/39 (71%)
Frame = -3 / -1
Query: 572 SKRCRWSPGRRAWWRSRWTCARTSASWTSWATGRWRWAA 456
S+RCRW PGR A WRS WTCA T A TSWA RWR AA
Sbjct: 456 SRRCRWRPGRPARWRSGWTCAGT*AWRTSWAAARWRSAA 340
Score = 68.9 bits (144), Expect(4) = 1e-029
Identities = 31/45 (68%), Positives = 35/45 (77%)
Frame = -1 / -2
Query: 715 QGRALRGPGVQRAPPGAERGQKVRRAHGVPVLVAAGGAQRAGEAP 581
+GR LRGPG++R PGAER + RRA G VLVAA GAQRAGEAP
Sbjct: 599 RGRPLRGPGLRREAPGAERRRGGRRALGAAVLVAAVGAQRAGEAP 465
Score = 29.5 bits (58), Expect(4) = 1e-029
Identities = 12/20 (60%), Positives = 13/20 (65%)
Frame = -1 / -2
Query: 457 PHAARRGPQAHAQLGSLMAE 398
PHAARR PQAH L+ E
Sbjct: 341 PHAARRRPQAHRGTTGLIKE 282
Score = 24.0 bits (46), Expect(2) = 2e-024
Identities = 11/29 (37%), Positives = 15/29 (51%)
Frame = +2 / +1
Query: 182 IVITITNRPSNESNASKQMTKSCPWIQFF 268
IV I N + + ++S PWIQFF
Sbjct: 61 IVPPIPNESNQI*QPQQNNSQSPPWIQFF 147
Score = 22.1 bits (42), Expect(4) = 1e-029
Identities = 7/8 (87%), Positives = 7/8 (87%)
Frame = -2 / -2
Query: 267 KNWIHGQL 244
KNWIHG L
Sbjct: 146 KNWIHGGL 123
>gb|CK208141.1|CK208141 FGAS019822 Triticum aestivum FGAS: Library 5 GATE 7 Triticum
aestivum cDNA, mRNA sequence
Length = 1134
Score = 171 bits (368), Expect = 8e-042
Identities = 74/102 (72%), Positives = 82/102 (80%)
Frame = -2 / -1
Query: 711 GALCEGLAFNVPLRVRNAGRRSGGPTVFLFSSPPAVPNAPAKPLLGFEKVSLEPGQAGVV 532
G CE LAF+V LRVRN G +G +V LFSSPP+ NAPAK LLGFEKVSL PG+AG V
Sbjct: 576 GDHCEDLAFDVKLRVRNPGEVAGAHSVLLFSSPPSAHNAPAKHLLGFEKVSLAPGEAGTV 397
Query: 531 AFKVDVCKDLSVVDELGNRKVALGSHTLHVGDLKHTLNLGV* 406
AF+VDVC+DLSV DELG RKVALG HTLHVGDLKHT+ L V*
Sbjct: 396 AFRVDVCRDLSVADELGGRKVALGGHTLHVGDLKHTVELRV* 271
Score = 109 bits (233), Expect = 3e-023
Identities = 51/102 (50%), Positives = 55/102 (53%)
Frame = +2 / +2
Query: 407 QTPKLSVCLRSPTCSVWXXXXXXXXXXXXXXXXXXXXXXXNATTPACPGSSDTFSKPSRG 586
QT +VCLRSPTCSVW NAT PA PG+SDTFS PSR
Sbjct: 272 QTRSSTVCLRSPTCSVWPPSATLRPPSSSATLRSLHTSTLNATVPASPGASDTFSNPSRC 451
Query: 587 FAGALGTAGGDENRNTVGPPDLLPAFRTRRGTLNARPSQSAP 712
FAGAL GGDEN +T P P FRTR T AR SQ +P
Sbjct: 452 FAGALCADGGDENSSTECAPATSPGFRTRSFTSKARSSQWSP 577
Score = 69.8 bits (146), Expect(3) = 5e-028
Identities = 27/39 (69%), Positives = 28/39 (71%)
Frame = -3 / -2
Query: 572 SKRCRWSPGRRAWWRSRWTCARTSASWTSWATGRWRWAA 456
S+RCRW PGR A WRS WTCA T A TSWA RWR AA
Sbjct: 437 SRRCRWRPGRPARWRSGWTCAGT*AWRTSWAAARWRSAA 321
Score = 63.8 bits (133), Expect(3) = 5e-028
Identities = 29/45 (64%), Positives = 33/45 (73%)
Frame = -1 / -3
Query: 715 QGRALRGPGVQRAPPGAERGQKVRRAHGVPVLVAAGGAQRAGEAP 581
+GR LRGPG++R PGAE + RRA G V VAA GAQRAGEAP
Sbjct: 580 RGRPLRGPGLRREAPGAEPRRGGRRALGAAVFVAAVGAQRAGEAP 446
Score = 29.5 bits (58), Expect(3) = 5e-028
Identities = 12/20 (60%), Positives = 13/20 (65%)
Frame = -1 / -3
Query: 457 PHAARRGPQAHAQLGSLMAE 398
PHAARR PQAH L+ E
Sbjct: 322 PHAARRRPQAHRGTTGLIKE 263
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 695,287,861
Number of Sequences: 636343
Number of extensions: 12690175
Number of successful extensions: 313920
Number of sequences better than 1.0e-020: 3
length of database: 122,413,413
effective HSP length: 53
effective length of database: 88,687,234
effective search space used: 16318451056
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)