TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2591368.2.1
(543 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|U42336.1|TAU42336 Triticum aestivum ACC synthase (TA-ACS... 135 1e-033
gb|CN013072.1|CN013072 WHE3956_D11_G22ZS Wheat Fusarium gra... 136 2e-033
gb|CA500522.1|CA500522 WHE4020_G10_N20ZT Wheat meiotic anth... 133 1e-032
gb|CD373768.1|CD373768 WHE2601_G01_M01ZT Sumai3 Wheat Fusar... 117 2e-028
gb|BE401232.1|BE401232 CNW01PL0232 ITEC CNW Wheat Powdery M... 69 4e-023
>gb|U42336.1|TAU42336 Triticum aestivum ACC synthase (TA-ACS2) gene, complete cds
Length = 5590
Score = 135 bits (289), Expect(3) = 1e-033
Identities = 54/70 (77%), Positives = 58/70 (82%)
Frame = -1 / +3
Query: 543 RVKLNVSPGTSFHCNEPGWFRVCYANMDDDTMEVALDRIRRFVRQHQHSKAKAERWAATR 364
+VKLNVSPGTSFHC EPGWFRVC+ANMDD+TM VAL RIR FVRQHQ KAKA+RWAA
Sbjct: 2679 KVKLNVSPGTSFHCGEPGWFRVCHANMDDETMGVALSRIRDFVRQHQQQKAKAQRWAARS 2858
Query: 363 PLRLSLPRRG 334
L LSL R G
Sbjct: 2859 HLHLSLQRHG 2888
Score = 98.7 bits (209), Expect(2) = 7e-022
Identities = 40/58 (68%), Positives = 41/58 (70%)
Frame = +2 / -1
Query: 368 VAAQRSALALLCWCWRTKRRIRXXXXXXXXXXMLA*QTRNQPGSLQWNDVPGDTLSFT 541
+AAQR ALA CWCWRTK RIR MLA QTRN PGS QWNDVPGDTLSFT
Sbjct: 2854 LAAQRWALAFCCWCWRTKSRIRLSATPMVSSSMLAWQTRNHPGSPQWNDVPGDTLSFT 2681
Score = 25.4 bits (49), Expect(2) = 7e-022
Identities = 8/20 (40%), Positives = 8/20 (40%)
Frame = +3 / -2
Query: 255 SWRGPSGTAATATPRGWGWR 314
SWR R WGWR
Sbjct: 2973 SWRRGRAETGATAARPWGWR 2914
Score = 23.5 bits (45), Expect(3) = 1e-033
Identities = 9/12 (75%), Positives = 9/12 (75%)
Frame = -2 / +1
Query: 293 RCCRRSPRWSTP 258
RCCR S R STP
Sbjct: 2932 RCCRPSLRSSTP 2967
Score = 22.6 bits (43), Expect(3) = 1e-033
Identities = 8/13 (61%), Positives = 9/13 (69%)
Frame = -2 / +1
Query: 317 TSPSPAPWRCCRR 279
T+ SPAPW C R
Sbjct: 2905 TTRSPAPWPRCCR 2943
>gb|CN013072.1|CN013072 WHE3956_D11_G22ZS Wheat Fusarium graminearum infected spike cDNA
library Triticum aestivum cDNA clone WHE3956_D11_G22,
mRNA sequence
Length = 695
Score = 136 bits (292), Expect(3) = 2e-033
Identities = 55/71 (77%), Positives = 59/71 (83%)
Frame = -1 / +3
Query: 543 RVKLNVSPGTSFHCNEPGWFRVCYANMDDDTMEVALDRIRRFVRQHQHSKAKAERWAATR 364
+VKLNVSPGTSFHC EPGWFRVC+ANMDD+TM VAL RIR FVRQHQ KAKA+RWAA
Sbjct: 21 KVKLNVSPGTSFHCGEPGWFRVCHANMDDETMGVALSRIRDFVRQHQQQKAKAQRWAARG 200
Query: 363 PLRLSLPRRGA 331
L LSL R GA
Sbjct: 201 HLHLSLQRHGA 233
Score = 100 bits (212), Expect(2) = 3e-022
Identities = 47/85 (55%), Positives = 49/85 (57%)
Frame = +2 / -2
Query: 287 SNAKGLGMARCEAVVAPXXXXXXXXXXVAAQRSALALLCWCWRTKRRIRXXXXXXXXXXM 466
S A GL A +AP +AAQR ALA CWCWRTK RIR M
Sbjct: 277 SAAMGLESAWYCEAMAPWRCRLRWRCPLAAQRWALAFCCWCWRTKSRIRLSATPMVSSSM 98
Query: 467 LA*QTRNQPGSLQWNDVPGDTLSFT 541
LA QTRN PGS QWNDVPGDTLSFT
Sbjct: 97 LAWQTRNHPGSPQWNDVPGDTLSFT 23
Score = 25.4 bits (49), Expect(2) = 3e-022
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = +2 / -2
Query: 260 AWTIGDCGDSNA 295
AWT GD GDS+A
Sbjct: 307 AWTSGDWGDSSA 272
Score = 24.0 bits (46), Expect(3) = 2e-033
Identities = 8/12 (66%), Positives = 8/12 (66%)
Frame = -2 / +1
Query: 293 RCCRRSPRWSTP 258
RCC S RWS P
Sbjct: 274 RCCPPSLRWSMP 309
Score = 21.7 bits (41), Expect(3) = 2e-033
Identities = 7/11 (63%), Positives = 8/11 (72%)
Frame = -2 / +1
Query: 317 TSPSPAPWRCC 285
T+ SPAPW C
Sbjct: 247 TTRSPAPWPRC 279
>gb|CA500522.1|CA500522 WHE4020_G10_N20ZT Wheat meiotic anther cDNA library Triticum
aestivum cDNA clone WHE4020_G10_N20, mRNA sequence
Length = 637
Score = 133 bits (286), Expect(3) = 1e-032
Identities = 54/69 (78%), Positives = 57/69 (82%)
Frame = -1 / +1
Query: 537 KLNVSPGTSFHCNEPGWFRVCYANMDDDTMEVALDRIRRFVRQHQHSKAKAERWAATRPL 358
KLNVSPGTSFHC EPGWFRVC+ANMDD+TM VAL RIR FVRQHQ KAKA+RWAA L
Sbjct: 13 KLNVSPGTSFHCGEPGWFRVCHANMDDETMGVALSRIRDFVRQHQQQKAKAQRWAARGHL 192
Query: 357 RLSLPRRGA 331
LSL R GA
Sbjct: 193 HLSLQRHGA 219
Score = 97.7 bits (207), Expect(2) = 1e-021
Identities = 46/84 (54%), Positives = 48/84 (57%)
Frame = +2 / -3
Query: 287 SNAKGLGMARCEAVVAPXXXXXXXXXXVAAQRSALALLCWCWRTKRRIRXXXXXXXXXXM 466
S A GL A +AP +AAQR ALA CWCWRTK RIR M
Sbjct: 263 SAAMGLESAWYCEAMAPWRCRLRWRCPLAAQRWALAFCCWCWRTKSRIRLSATPMVSSSM 84
Query: 467 LA*QTRNQPGSLQWNDVPGDTLSF 538
LA QTRN PGS QWNDVPGDTLSF
Sbjct: 83 LAWQTRNHPGSPQWNDVPGDTLSF 12
Score = 25.4 bits (49), Expect(2) = 1e-021
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = +2 / -3
Query: 260 AWTIGDCGDSNA 295
AWT GD GDS+A
Sbjct: 293 AWTSGDWGDSSA 258
Score = 24.0 bits (46), Expect(3) = 1e-032
Identities = 8/12 (66%), Positives = 8/12 (66%)
Frame = -2 / +2
Query: 293 RCCRRSPRWSTP 258
RCC S RWS P
Sbjct: 260 RCCPPSLRWSMP 295
Score = 21.7 bits (41), Expect(3) = 1e-032
Identities = 7/11 (63%), Positives = 8/11 (72%)
Frame = -2 / +2
Query: 317 TSPSPAPWRCC 285
T+ SPAPW C
Sbjct: 233 TTRSPAPWPRC 265
>gb|CD373768.1|CD373768 WHE2601_G01_M01ZT Sumai3 Wheat Fusarium graminearum infected spike
cDNA library Triticum aestivum cDNA clone
WHE2601_G01_M01, mRNA sequence
Length = 724
Score = 117 bits (251), Expect(3) = 2e-028
Identities = 48/64 (75%), Positives = 51/64 (79%)
Frame = -1 / -3
Query: 522 PGTSFHCNEPGWFRVCYANMDDDTMEVALDRIRRFVRQHQHSKAKAERWAATRPLRLSLP 343
PGTSFHC EPG FRVC+ANMDD+TM VAL RIR FVRQHQ KAKA+RWAA L LSL
Sbjct: 722 PGTSFHCGEPGCFRVCHANMDDETMGVALSRIRDFVRQHQQQKAKAQRWAARSHLHLSLQ 543
Query: 342 RRGA 331
R GA
Sbjct: 542 RHGA 531
Score = 23.5 bits (45), Expect(3) = 2e-028
Identities = 9/12 (75%), Positives = 9/12 (75%)
Frame = -2 / -1
Query: 293 RCCRRSPRWSTP 258
RCCR S R STP
Sbjct: 490 RCCRPSLRSSTP 455
Score = 22.6 bits (43), Expect(3) = 2e-028
Identities = 8/13 (61%), Positives = 9/13 (69%)
Frame = -2 / -1
Query: 317 TSPSPAPWRCCRR 279
T+ SPAPW C R
Sbjct: 517 TTRSPAPWPRCCR 479
>gb|BE401232.1|BE401232 CNW01PL0232 ITEC CNW Wheat Powdery Mildew Resistant Library
Triticum aestivum cDNA clone CNW01PL0232, mRNA sequence
Length = 300
Score = 68.9 bits (144), Expect(3) = 4e-023
Identities = 30/45 (66%), Positives = 35/45 (77%)
Frame = -1 / +3
Query: 474 YANMDDDTMEVALDRIRRFVRQHQHSKAKAERWAATRPLRLSLPR 340
+ANMDD+TM VAL+RIR FVRQH+ KAKA+ WAA L LSL R
Sbjct: 126 HANMDDETMGVALNRIRDFVRQHRQQKAKAQCWAARGHLHLSLQR 260
Score = 55.6 bits (115), Expect(3) = 4e-023
Identities = 19/21 (90%), Positives = 19/21 (90%)
Frame = -1 / +1
Query: 537 KLNVSPGTSFHCNEPGWFRVC 475
KLNVSPGT FHC EPGWFRVC
Sbjct: 64 KLNVSPGTLFHCREPGWFRVC 126
Score = 19.4 bits (36), Expect(3) = 4e-023
Identities = 6/14 (42%), Positives = 8/14 (57%)
Frame = -2 / +2
Query: 329 PPLRTSPSPAPWRC 288
PP ++ APW C
Sbjct: 239 PPPQSPAPLAPWLC 280
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 458,467,176
Number of Sequences: 636343
Number of extensions: 7112593
Number of successful extensions: 247561
Number of sequences better than 1.0e-020: 6
length of database: 122,413,413
effective HSP length: 52
effective length of database: 89,323,577
effective search space used: 11433417856
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)