TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2448489.2.1
(677 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ805546.1|BQ805546 WHE3568_C05_E10ZS Wheat developing g... 265 4e-070
gb|BE415956.1|BE415956 MUG002.G09R990520 ITEC MUG Wheat Spi... 187 1e-046
gb|CA605379.1|CA605379 wr1.pk0053.e2 wr1 Triticum aestivum ... 184 7e-046
gb|BE417540.1|BE417540 MUG022.A11R990620 ITEC MUG Wheat Spi... 180 1e-044
gb|BQ839503.1|BQ839503 WHE4166_H04_P08ZS Wheat CS whole pla... 166 3e-040
gb|CA652956.1|CA652956 wre1n.pk156.f8 wre1n Triticum aestiv... 163 2e-039
gb|CK194450.1|CK194450 FGAS002880 Triticum aestivum FGAS: L... 148 6e-035
gb|CK157787.1|CK157787 FGAS038954 Triticum aestivum FGAS: T... 125 8e-029
gb|CA691627.1|CA691627 wlm96.pk049.e5 wlm96 Triticum aestiv... 118 7e-026
>gb|BQ805546.1|BQ805546 WHE3568_C05_E10ZS Wheat developing grains cDNA library Triticum
aestivum cDNA clone WHE3568_C05_E10, mRNA sequence
Length = 721
Score = 265 bits (573), Expect = 4e-070
Identities = 104/130 (80%), Positives = 113/130 (86%)
Frame = -2 / +2
Query: 661 LSQXDXXXXKVFIENSYYXHGFWAXXVDKYXXMMENHXXGLGDERWPFVTHFVGCKPCGS 482
LSQ + KV+IENSYY HGFWA VDKY MMENH GLGDERWPFVTHFVGCKPCGS
Sbjct: 65 LSQKEKWMDKVYIENSYYLHGFWAGLVDKYEEMMENHHPGLGDERWPFVTHFVGCKPCGS 244
Query: 481 YGDYPVERCLRSMERAFNFADNQVLRLYGFSHKGLESPKIKRFRDQTTRPINDVENLDMK 302
YGDYPV+RCL+SMERAFNFADNQVLRLYGF+HKGLESPKIKR R QTT+PIND ENLD+K
Sbjct: 245 YGDYPVDRCLKSMERAFNFADNQVLRLYGFAHKGLESPKIKRIRSQTTKPINDKENLDVK 424
Query: 301 AKISTIS*KF 272
AK+ST S* +
Sbjct: 425 AKMSTTS*SY 454
Score = 166 bits (358), Expect(3) = 5e-045
Identities = 73/97 (75%), Positives = 76/97 (78%)
Frame = +3 / -2
Query: 387 CEKPYSRSTWLSAKLNARSMLLRQRSTG*SP*LPHGLHPTK*VTKGHRSSPRPXW*FSII 566
C KPY RSTWLSAKLNARS+LLR RSTG*SP*LPHGLHPTK VT GHRSSP P W FSII
Sbjct: 339 CAKPYRRSTWLSAKLNARSILLRHRSTG*SP*LPHGLHPTKCVTNGHRSSPSPGWWFSII 160
Query: 567 XSYLSTXXAQKPCX**EFSMNTLXXXXSFCESXXXIS 677
SYLST AQKPC * EFS+ TL SFCES I+
Sbjct: 159 SSYLSTSPAQKPCK*YEFSIYTLSIHFSFCESKRCIN 49
Score = 152 bits (327), Expect = 3e-036
Identities = 72/138 (52%), Positives = 89/138 (64%)
Frame = +2 / -1
Query: 263 LSSELSGNCRYFGLHI*VLHIINWSSCLIPESLDLGAFQPLV*ETI*PKHLVVGKIKRSL 442
LSS S + R+ GLHI VL ++N L P+ DL AFQPLV ETI +HLV+GKIK SL
Sbjct: 463 LSSVASRSGRHLGLHIKVLLVVNRLCGLTPDPFDLRAFQPLVCETIQAQHLVIGKIKCSL 284
Query: 443 HAPEAALHWVIPIAPTWLAPNKVSHERPPLVAEAXXVILHHLXILIXXXSPEAMXVVGIL 622
H E ++WVI IA WLAPNKV H+ PPL+ + V+LHHL +L+ SPE + VV IL
Sbjct: 283 HTLETPVYWVITIASAWLAPNKVCHKWPPLIPKPWMVVLHHLLVLVHQPSPETVQVV*IL 104
Query: 623 NEHLVXXXVXLREXXXXQ 676
HLV + LRE Q
Sbjct: 103 YIHLVHPLLLLREQEVYQ 50
Score = 133 bits (286), Expect = 1e-030
Identities = 68/134 (50%), Positives = 83/134 (61%)
Frame = -1 / +1
Query: 674 DXXXALAKXXXXXQGVH*EFXXXXXXLGXVGX*V*XDDGESXXXXXXRAVAFRDSLCWVQ 495
D ALA+ QGV+ EF LG G V DDGE AVA D+LCWVQ
Sbjct: 52 DTPLALAEGEVDGQGVYREFILLARFLGWAGGQVRGDDGEPPSRAWG*AVAICDTLCWVQ 231
Query: 494 AMWELWGLPSGALPQEHGASV*FCRQPGASAIWFLTQGAGKPQDQEIQGSDN*TN**CGE 315
AM +LW LPS + QE+GAS+*FCR PGA+ +WF TQGAGKP DQ+ Q SD+ * GE
Sbjct: 232 AMRKLW*LPSRPVSQEYGASI*FCR*PGAAPVWFRTQGAGKP*DQKDQESDHKAY*RQGE 411
Query: 314 LRYEGQNIYNFLKV 273
EGQ++ +FLK+
Sbjct: 412 P*CEGQDVDHFLKL 453
Score = 130 bits (279), Expect = 1e-029
Identities = 65/110 (59%), Positives = 69/110 (62%)
Frame = +1 / -3
Query: 346 DP*IS*SWGFPAPCVRNHIAEAPGCRQN*TLAPCS*GSAPLGNPHSSHMACTQQSESRKA 525
D * *S GFPAPCVRNH APG RQN* LAP S* + LGN HS MACTQQS S+ A
Sbjct: 380 DS*SF*S*GFPAPCVRNHTGAAPGYRQN*MLAPYS*DTGLLGNHHSFRMACTQQSVSQMA 201
Query: 526 TARRRGLXGDSPSSXHTYXPTQPRSHASSRNSQ*TPCXXXXXFARAXXXS 675
TA + L G SPSS T P QPR+ ASS NS TPC ARA S
Sbjct: 200 TAHPQALDGGSPSSPRTCPPAQPRNRASSMNSLYTPCPSTSPSARARGVS 51
Score = 84.9 bits (179), Expect(2) = 1e-021
Identities = 39/76 (51%), Positives = 48/76 (63%)
Frame = -3 / +3
Query: 492 HVGAMGITQWSAASGAWSERLILPTTRCFGYMVSHTRGWKAPRSRDSGIRQLDQLMMWRT 313
H AM ITQ + S WSE LILP TRC MVSHTRGWKA RS+ SG+R L RT
Sbjct: 234 HAEAMVITQ*TGVSRVWSEHLILPITRCCACMVSHTRGWKALRSKGSGVRPQSLLTTRRT 413
Query: 312 *I*RPKYLQFPESSDE 265
+*RP+ E++++
Sbjct: 414 LM*RPRCRPLLEATED 461
Score = 38.6 bits (78), Expect(2) = 1e-021
Identities = 19/38 (50%), Positives = 20/38 (52%)
Frame = -3 / +3
Query: 663 CSRKXTXXXTRCSLRIPTTXMASGLXXXISMXR*WRIT 550
CSR+ TRC RI TT SGL S R*WR T
Sbjct: 63 CSRRRRSGWTRCI*RIHTTCTVSGLGWWTSTRR*WRTT 176
Score = 28.6 bits (56), Expect(3) = 5e-045
Identities = 11/14 (78%), Positives = 13/14 (92%)
Frame = +3 / -2
Query: 279 QEIVDILAFISKFS 320
QE+VDILAF S+FS
Sbjct: 447 QEVVDILAFTSRFS 406
Score = 27.6 bits (54), Expect(3) = 5e-045
Identities = 9/14 (64%), Positives = 11/14 (78%)
Frame = +3 / -3
Query: 39 KARLFPSDLSFYHV 80
K +FPSDLS YH+
Sbjct: 647 KTEIFPSDLSSYHI 606
>gb|BE415956.1|BE415956 MUG002.G09R990520 ITEC MUG Wheat Spikelet Library Triticum aestivum
cDNA clone MUG002.G09, mRNA sequence
Length = 989
Score = 187 bits (402), Expect = 1e-046
Identities = 66/123 (53%), Positives = 88/123 (71%)
Frame = -2 / +3
Query: 658 SQXDXXXXKVFIENSYYXHGFWAXXVDKYXXMMENHXXGLGDERWPFVTHFVGCKPCGSY 479
+Q + KV++EN YY HG+W VD+Y M+EN+ GLGD RWP VTHFVGCKPC +
Sbjct: 285 TQREKWGNKVYLENGYYLHGYWGILVDRYEEMLENYQPGLGDHRWPLVTHFVGCKPCSKF 464
Query: 478 GDYPVERCLRSMERAFNFADNQVLRLYGFSHKGLESPKIKRFRDQTTRPINDVENLDMKA 299
GDYPVERCL+ M+RAFNF DNQVL +YGF HK L +I+R R++T+ P++ ++
Sbjct: 465 GDYPVERCLKQMDRAFNFGDNQVLHMYGFEHKSLGEQRIQRIRNETSEPLDMKDDYGCSP 644
Query: 298 KIS 290
K+S
Sbjct: 645 KVS 653
Score = 120 bits (256), Expect = 2e-026
Identities = 56/83 (67%), Positives = 58/83 (69%)
Frame = +3 / -2
Query: 387 CEKPYSRSTWLSAKLNARSMLLRQRSTG*SP*LPHGLHPTK*VTKGHRSSPRPXW*FSII 566
C PY +TWLS KL ARS+ LR RSTG*SP L HGLHPTK*VT GHR SP P W*FS I
Sbjct: 556 CSNPYMCNTWLSPKLKARSICLRHRSTG*SPNLEHGLHPTK*VTSGHR*SPSPGW*FSSI 377
Query: 567 XSYLSTXXAQKPCX**EFSMNTL 635
SYLST Q P ** FS TL
Sbjct: 376 SSYLSTKMPQ*PWR**PFSRYTL 308
>gb|CA605379.1|CA605379 wr1.pk0053.e2 wr1 Triticum aestivum cDNA clone wr1.pk0053.e2 5'
end, mRNA sequence
Length = 584
Score = 184 bits (397), Expect = 7e-046
Identities = 65/101 (64%), Positives = 79/101 (78%)
Frame = -2 / +3
Query: 634 KVFIENSYYXHGFWAXXVDKYXXMMENHXXGLGDERWPFVTHFVGCKPCGSYGDYPVERC 455
KV++EN YY HG+W VD+Y M+EN+ GLGD RWP VTHFVGCKPC +GDYPVERC
Sbjct: 21 KVYLENGYYLHGYWGILVDRYEEMLENYQPGLGDHRWPLVTHFVGCKPCSKFGDYPVERC 200
Query: 454 LRSMERAFNFADNQVLRLYGFSHKGLESPKIKRFRDQTTRP 332
L+ M+RAFNF DNQVL +YGF HK L S +IKR R++T+ P
Sbjct: 201 LKQMDRAFNFGDNQVLHMYGFEHKSLASRRIKRIRNETSDP 323
Score = 120 bits (256), Expect = 2e-026
Identities = 56/83 (67%), Positives = 58/83 (69%)
Frame = +3 / -2
Query: 387 CEKPYSRSTWLSAKLNARSMLLRQRSTG*SP*LPHGLHPTK*VTKGHRSSPRPXW*FSII 566
C PY +TWLS KL ARS+ LR RSTG*SP L HGLHPTK*VT GHR SP P W*FS I
Sbjct: 268 CSNPYMCNTWLSPKLKARSICLRHRSTG*SPNLEHGLHPTK*VTSGHR*SPSPGW*FSSI 89
Query: 567 XSYLSTXXAQKPCX**EFSMNTL 635
SYLST Q P ** FS TL
Sbjct: 88 SSYLSTKIPQ*PWR**PFSRYTL 20
>gb|BE417540.1|BE417540 MUG022.A11R990620 ITEC MUG Wheat Spikelet Library Triticum aestivum
cDNA clone MUG022.A11, mRNA sequence
Length = 600
Score = 180 bits (388), Expect = 1e-044
Identities = 64/105 (60%), Positives = 79/105 (75%)
Frame = -2 / +3
Query: 658 SQXDXXXXKVFIENSYYXHGFWAXXVDKYXXMMENHXXGLGDERWPFVTHFVGCKPCGSY 479
+Q + KV++EN YY HG+W VD+Y M+EN+ GLGD RWP VTHFVGCKPC +
Sbjct: 285 TQREKWGNKVYLENGYYLHGYWGILVDRYEEMLENYQPGLGDHRWPLVTHFVGCKPCSKF 464
Query: 478 GDYPVERCLRSMERAFNFADNQVLRLYGFSHKGLESPKIKRFRDQ 344
GDYPVERCL+ M+RAFNF DNQVL +YGF HK L S +IKR R++
Sbjct: 465 GDYPVERCLKQMDRAFNFGDNQVLHMYGFEHKSLASRRIKRIRNE 599
Score = 120 bits (256), Expect = 2e-026
Identities = 56/83 (67%), Positives = 58/83 (69%)
Frame = +3 / -3
Query: 387 CEKPYSRSTWLSAKLNARSMLLRQRSTG*SP*LPHGLHPTK*VTKGHRSSPRPXW*FSII 566
C PY +TWLS KL ARS+ LR RSTG*SP L HGLHPTK*VT GHR SP P W*FS I
Sbjct: 556 CSNPYMCNTWLSPKLKARSICLRHRSTG*SPNLEHGLHPTK*VTSGHR*SPSPGW*FSSI 377
Query: 567 XSYLSTXXAQKPCX**EFSMNTL 635
SYLST Q P ** FS TL
Sbjct: 376 SSYLSTKMPQ*PWR**PFSRYTL 308
>gb|BQ839503.1|BQ839503 WHE4166_H04_P08ZS Wheat CS whole plant cDNA library Triticum
aestivum cDNA clone WHE4166_H04_P08, mRNA sequence
Length = 552
Score = 166 bits (356), Expect = 3e-040
Identities = 58/93 (62%), Positives = 72/93 (77%)
Frame = -2 / -3
Query: 604 HGFWAXXVDKYXXMMENHXXGLGDERWPFVTHFVGCKPCGSYGDYPVERCLRSMERAFNF 425
HG+W VD+Y M+EN+ GLGD RWP VTHFVGCKPC +GDYPVERCL+ M+RAFNF
Sbjct: 550 HGYWGILVDRYEEMLENYQPGLGDHRWPLVTHFVGCKPCSKFGDYPVERCLKQMDRAFNF 371
Query: 424 ADNQVLRLYGFSHKGLESPKIKRFRDQTTRPIN 326
DNQVL +YGF HK L S +IKR R++ + P++
Sbjct: 370 GDNQVLHMYGFEHKSLASRRIKRIRNEPSDPLD 272
Score = 113 bits (242), Expect = 2e-024
Identities = 50/72 (69%), Positives = 52/72 (72%)
Frame = +3 / +3
Query: 387 CEKPYSRSTWLSAKLNARSMLLRQRSTG*SP*LPHGLHPTK*VTKGHRSSPRPXW*FSII 566
C PY +TWLS KL ARS+ LR RSTG*SP L HGLHPTK*VT GHR SP P W*FS I
Sbjct: 333 CSNPYMCNTWLSPKLKARSICLRHRSTG*SPNLEHGLHPTK*VTSGHR*SPSPGW*FSSI 512
Query: 567 XSYLSTXXAQKP 602
SYLST Q P
Sbjct: 513 SSYLSTKIPQ*P 548
>gb|CA652956.1|CA652956 wre1n.pk156.f8 wre1n Triticum aestivum cDNA clone wre1n.pk156.f8 5'
end, mRNA sequence
Length = 529
Score = 163 bits (351), Expect = 2e-039
Identities = 62/89 (69%), Positives = 71/89 (79%)
Frame = -2 / +1
Query: 583 VDKYXXMMENHXXGLGDERWPFVTHFVGCKPCGSYGDYPVERCLRSMERAFNFADNQVLR 404
VDKY MME H GLGDERWPF+THFVG KPCGSYGDYPVE+CL MERAFNFADNQVLR
Sbjct: 4 VDKYEEMMEKHHPGLGDERWPFITHFVGXKPCGSYGDYPVEQCLTGMERAFNFADNQVLR 183
Query: 403 LYGFSHKGLESPKIKRFRDQTTRPINDVE 317
LYGF H+ L +PK+K ++T P+ + E
Sbjct: 184 LYGFRHRSLTNPKVKPVANRTASPLLNKE 270
Score = 110 bits (235), Expect = 2e-023
Identities = 50/66 (75%), Positives = 51/66 (77%)
Frame = +3 / -3
Query: 387 CEKPYSRSTWLSAKLNARSMLLRQRSTG*SP*LPHGLHPTK*VTKGHRSSPRPXW*FSII 566
C KP S STWLSAKL ARSM +R STG*SP*LP GL PTK V KGHRSSP P W FSII
Sbjct: 200 CRKP*SLSTWLSAKLKARSMPVRHCSTG*SP*LPQGLXPTKCVMKGHRSSPSPGWCFSII 21
Query: 567 XSYLST 584
SYLST
Sbjct: 20 SSYLST 3
>gb|CK194450.1|CK194450 FGAS002880 Triticum aestivum FGAS: Library 3 Gate 6 Triticum
aestivum cDNA, mRNA sequence
Length = 828
Score = 148 bits (318), Expect = 6e-035
Identities = 55/85 (64%), Positives = 66/85 (77%)
Frame = -2 / -2
Query: 583 VDKYXXMMENHXXGLGDERWPFVTHFVGCKPCGSYGDYPVERCLRSMERAFNFADNQVLR 404
V+KY MME H GLG+ERWPF+T FVGCKP G YG+YPVE+CL MERAF FADNQVLR
Sbjct: 695 VNKYEEMMEKHHPGLGEERWPFITQFVGCKPSGRYGEYPVEQCLTGMERAFKFADNQVLR 516
Query: 403 LYGFSHKGLESPKIKRFRDQTTRPI 329
LYGF H+ L +PK+K ++T P+
Sbjct: 515 LYGFRHRSLTNPKVKPVANRTGNPL 441
>gb|CK157787.1|CK157787 FGAS038954 Triticum aestivum FGAS: TaLt4 Triticum aestivum cDNA,
mRNA sequence
Length = 847
Score = 125 bits (267), Expect(2) = 8e-029
Identities = 44/67 (65%), Positives = 54/67 (80%)
Frame = -2 / -3
Query: 526 WPFVTHFVGCKPCGSYGDYPVERCLRSMERAFNFADNQVLRLYGFSHKGLESPKIKRFRD 347
WP VT FVGCKPC +GDYPVERCL+ M+RAFNF DNQVL +YGF HK L S +IKR R+
Sbjct: 605 WPLVTRFVGCKPCSKFGDYPVERCLKQMDRAFNFGDNQVLHMYGFEHKSLASRRIKRIRN 426
Query: 346 QTTRPIN 326
+T+ P++
Sbjct: 425 ETSDPLD 405
Score = 22.6 bits (43), Expect(2) = 8e-029
Identities = 8/14 (57%), Positives = 9/14 (64%)
Frame = -2 / -2
Query: 574 YXXMMENHXXGLGD 533
Y M EN+ GLGD
Sbjct: 651 YEEMFENYPAGLGD 610
>gb|CA691627.1|CA691627 wlm96.pk049.e5 wlm96 Triticum aestivum cDNA clone wlm96.pk049.e5 5'
end, mRNA sequence
Length = 494
Score = 118 bits (252), Expect = 7e-026
Identities = 41/69 (59%), Positives = 49/69 (71%)
Frame = -2 / +2
Query: 631 VFIENSYYXHGFWAXXVDKYXXMMENHXXGLGDERWPFVTHFVGCKPCGSYGDYPVERCL 452
V++EN YY HG+W V +Y M+ N+ GLGD RW VTHFVGCKPC +GDYPVERCL
Sbjct: 158 VYLENGYYLHGYWGILVXRYXEMLXNYQPGLGDHRWXLVTHFVGCKPCSKFGDYPVERCL 337
Query: 451 RSMERAFNF 425
M+R F
Sbjct: 338 XQMDRGLQF 364
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 558,233,603
Number of Sequences: 636343
Number of extensions: 7713010
Number of successful extensions: 70979
Number of sequences better than 1.0e-020: 15
length of database: 122,413,413
effective HSP length: 53
effective length of database: 88,687,234
effective search space used: 15254204248
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)