TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2404858.2.1
(958 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CD871730.1|CD871730 AZO2.118O23F010213 AZO2 Triticum aes... 148 4e-080
gb|BQ606611.1|BQ606611 BRY_2476 wheat EST endosperm library... 68 6e-027
gb|CK206078.1|CK206078 FGAS017654 Triticum aestivum FGAS: L... 58 3e-025
gb|BE414217.1|BE414217 SCU007.E04.R990714 ITEC SCU Wheat En... 46 2e-023
gb|CD926685.1|CD926685 GR45.001M10F001019 GR45 Triticum aes... 89 2e-022
gb|BJ248602.1|BJ248602 BJ248602 Y. Ogihara unpublished cDNA... 62 1e-021
>gb|CD871730.1|CD871730 AZO2.118O23F010213 AZO2 Triticum aestivum cDNA clone AZO2118O23,
mRNA sequence
Length = 721
Score = 148 bits (317), Expect(3) = 4e-080
Identities = 60/91 (65%), Positives = 69/91 (75%)
Frame = +1 / -3
Query: 1 RVCADLGAGAASYVHCDVTSEXXXXXXXXXXXXTFGALDIMFNNAGVSGPACYSVRESGK 180
R+CA+LG A+SYV CDVT E FG LDIMFNNAG+ G AC+S+R+S K
Sbjct: 635 RLCAELGPAASSYVRCDVTKEEDVAAAVDHTVARFGKLDIMFNNAGIGGAACHSIRDSTK 456
Query: 181 ADFERVLAVNLVGPFLGTKHAARVMVPSRRG 273
DFERVLAVNLVGPFLGTKHAARVMVP+R+G
Sbjct: 455 EDFERVLAVNLVGPFLGTKHAARVMVPARQG 363
Score = 120 bits (257), Expect(3) = 4e-080
Identities = 51/86 (59%), Positives = 55/86 (63%)
Frame = +1 / -3
Query: 268 RGGCIIXXXXXXXXXXXXXXXXXXCAKRAMVTLTENAAAELGQHGIRVNCVSPAGVATPL 447
R GCII CAKRA+V LTENAA ELGQHGIRVNC+SPA ATPL
Sbjct: 371 RQGCIIGTSSLASAVGGVASHAYTCAKRALVGLTENAATELGQHGIRVNCISPAAAATPL 192
Query: 448 TMRYMGLEAEALEQAMEGMANLKGVG 525
Y+GL+ EA E AME ANLKGVG
Sbjct: 191 ATGYVGLDGEAFEMAMESFANLKGVG 114
Score = 118 bits (252), Expect(3) = 2e-065
Identities = 55/83 (66%), Positives = 58/83 (69%)
Frame = -3 / +1
Query: 515 LRLAMPSIACSNASASRPMYLMVSGVATPAGETQFTRMPCWPSSAAAFSVRVTIARFAHA 336
LRLA S+A SNAS S P + SGVA AGE Q TR+PCWPSS AAFSV T AR AH
Sbjct: 124 LRLAKDSMAISNASPSSPT*PVASGVAAAAGEMQLTRIPCWPSSVAAFSVSPTRARLAHV 303
Query: 335 YAYDDTPVTAAATLDVPMMQPPR 267
YA D TP TA A LDVPMMQP R
Sbjct: 304 YACDATPPTADARLDVPMMQPCR 372
Score = 111 bits (238), Expect(3) = 2e-065
Identities = 54/92 (58%), Positives = 57/92 (61%)
Frame = -3 / +1
Query: 281 MQPPRREGTITRAACLVPRNGPTRFTASTRSKSALPLSRTL*HAGPLTPALLNMMSSAPN 102
M P R GT+TRAACLVPR GPTR TASTRSKS+L LSR L HA P PALLNMMSS PN
Sbjct: 355 MMQPCRAGTMTRAACLVPRKGPTRLTASTRSKSSLVLSRMLWHAAPPMPALLNMMSSFPN 534
Query: 101 VXXXXXXXXXXXXXXXXSQWT*LAAPAPRSAQ 6
+ S T*L A P SAQ
Sbjct: 535 LATVWSTAAATSSSLVTSHRT*LEAAGPSSAQ 630
Score = 83.1 bits (175), Expect(3) = 2e-032
Identities = 36/57 (63%), Positives = 41/57 (71%)
Frame = +3 / -2
Query: 111 AGHHVQQRGRQRAGVLQRPGERQG*LRARAGRESGGPVPRHQARGAGDGALAPRWLH 281
AGHHVQQR +R GV Q PG+ QG LRARAGR+ G +P +QARGAG GA A R H
Sbjct: 525 AGHHVQQRRHRRRGVPQHPGQHQGGLRARAGRQPRGALPGYQARGAGHGARAARLHH 355
Score = 70.3 bits (147), Expect(3) = 4e-080
Identities = 28/36 (77%), Positives = 32/36 (88%)
Frame = +1 / -3
Query: 529 LRVHDVAAAVRFLASDDARYISGHNLFVDGGISVAN 636
LRV D+AAAV FLA DDARY+SGHNL +DGGISV+N
Sbjct: 113 LRVKDIAAAVLFLACDDARYVSGHNLLIDGGISVSN 6
Score = 68.4 bits (143), Expect(3) = 2e-030
Identities = 31/54 (57%), Positives = 39/54 (72%)
Frame = -2 / +3
Query: 525 AHALEVGHALHCLLQRLRLEAHVPHGQRRGDPRGGDAVHANAVLAELRSRVLRQ 364
+HALEV LH L+RL ++ +V GQRRG R GDAV A+ +LA+LR RVLRQ
Sbjct: 114 SHALEVSKGLHGHLERLAVQPNVARGQRRGSRRRGDAVDADPMLAKLRRRVLRQ 275
Score = 67.0 bits (140), Expect(3) = 2e-032
Identities = 31/59 (52%), Positives = 34/59 (57%)
Frame = +3 / -2
Query: 276 LHHRDVERGGGSDWXXXXXXXXXEARDGDSDGERGCGARPARHSRELRLPRGGRHAADH 452
LHHRDVE G G +AR G +DGERG GA PA R+L LP GG HAA H
Sbjct: 363 LHHRDVEPGVGGGRRGVARVHVRQARPGRADGERGDGAWPAWDPRQLHLPGGGCHAAGH 187
Score = 62.5 bits (130), Expect(3) = 2e-030
Identities = 28/60 (46%), Positives = 31/60 (51%)
Frame = -2 / +3
Query: 282 DAATAARGHHHPRRVLGAEERAHQIHGQHALEVSXXXXXXXXXXXXXXXXXVEHDVQRAE 103
D A RGHH PRRVLG +E H++ GQHALEV VEHDVQ E
Sbjct: 354 DDAALPRGHHDPRRVLGTQEGPHEVDGQHALEVLLGAVPDAVARRAADAGVVEHDVQLPE 533
Score = 59.7 bits (124), Expect(3) = 2e-065
Identities = 25/34 (73%), Positives = 28/34 (82%)
Frame = -2 / +3
Query: 630 DGDPAVDEQVVAADVPRVIAGQEAHGGGDVVHPQ 529
DGD AVDEQVVAA V RVIAG++ H GGDV+ PQ
Sbjct: 12 DGDAAVDEQVVAAHVARVIAGEKEHRGGDVLDPQ 113
Score = 40.5 bits (82), Expect(3) = 2e-030
Identities = 17/25 (68%), Positives = 19/25 (76%)
Frame = -3 / +1
Query: 635 FATEIPPSTNRLWPLMYLASSLARK 561
F TE+PPS +RLWPL ASS ARK
Sbjct: 7 FETEMPPSMSRLWPLT*RASSQARK 81
Score = 28.1 bits (55), Expect(3) = 2e-032
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = +2 / -1
Query: 530 CGCTTSPPPCASWPAM 577
CG TSPP C+ PAM
Sbjct: 112 CGSRTSPPRCSFSPAM 65
>gb|BQ606611.1|BQ606611 BRY_2476 wheat EST endosperm library Triticum aestivum cDNA 5',
mRNA sequence
Length = 562
Score = 68.4 bits (143), Expect(3) = 6e-027
Identities = 29/59 (49%), Positives = 35/59 (59%)
Frame = +1 / +2
Query: 271 GGCIIXXXXXXXXXXXXXXXXXXCAKRAMVTLTENAAAELGQHGIRVNCVSPAGVATPL 447
GG I+ +K A+V LT+NAA ELG+HGIRVNCVSP GVATP+
Sbjct: 344 GGTIVSVASVAGVLGGMGPHAYTASKHALVGLTKNAACELGEHGIRVNCVSPFGVATPM 520
Score = 46.0 bits (94), Expect(3) = 6e-027
Identities = 20/48 (41%), Positives = 24/48 (50%)
Frame = +1 / +2
Query: 4 VCADLGAGAASYVHCDVTSEXXXXXXXXXXXXTFGALDIMFNNAGVSG 147
+ A LG SYVHCDV+ E G LD++ NNAGV G
Sbjct: 38 LAAALGGACCSYVHCDVSVEADVERAVGCCVARHGRLDVLCNNAGVLG 181
Score = 45.5 bits (93), Expect(3) = 6e-027
Identities = 19/32 (59%), Positives = 23/32 (71%)
Frame = +1 / +2
Query: 181 ADFERVLAVNLVGPFLGTKHAARVMVPSRRGG 276
A+F+RVL VN +G LG KHAAR M+ R GG
Sbjct: 242 AEFDRVLRVNTLGAALGMKHAARAMLQRRGGG 337
>gb|CK206078.1|CK206078 FGAS017654 Triticum aestivum FGAS: Library 5 GATE 7 Triticum
aestivum cDNA, mRNA sequence
Length = 1147
Score = 57.9 bits (120), Expect(4) = 3e-025
Identities = 24/52 (46%), Positives = 33/52 (63%)
Frame = +1 / +1
Query: 475 EALEQAMEGMANLKGVGALRVHDVAAAVRFLASDDARYISGHNLFVDGGISV 630
E L + + N G L V D+A A +LASD+A+Y++GHNL VDGG +V
Sbjct: 448 EELRRMVATAMNEMGGSVLEVEDIARAAVYLASDEAKYVNGHNLVVDGGFTV 603
Score = 45.1 bits (92), Expect(4) = 3e-025
Identities = 20/38 (52%), Positives = 23/38 (60%)
Frame = +1 / +1
Query: 160 SVRESGKADFERVLAVNLVGPFLGTKHAARVMVPSRRG 273
SV ADF+R +A N+ G KHAARVMVP R G
Sbjct: 121 SVASLDLADFDRTMAANVRSAVAGIKHAARVMVPRRSG 234
Score = 43.7 bits (89), Expect(4) = 3e-025
Identities = 17/40 (42%), Positives = 25/40 (62%)
Frame = +1 / +1
Query: 343 AKRAMVTLTENAAAELGQHGIRVNCVSPAGVATPLTMRYM 462
+K A++ A ELG+ G+RVN +SP +ATP +R M
Sbjct: 301 SKAAVIGAVRALAGELGRQGVRVNAISPHAIATPFGLRGM 420
Score = 24.4 bits (47), Expect(4) = 3e-025
Identities = 8/14 (57%), Positives = 12/14 (85%)
Frame = +1 / +1
Query: 106 GALDIMFNNAGVSG 147
G LD++ N+AGV+G
Sbjct: 64 GRLDVLHNHAGVTG 105
>gb|BE414217.1|BE414217 SCU007.E04.R990714 ITEC SCU Wheat Endosperm Library Triticum
aestivum cDNA clone SCU007.E04, mRNA sequence
Length = 682
Score = 46.0 bits (94), Expect(4) = 2e-023
Identities = 20/48 (41%), Positives = 24/48 (50%)
Frame = +1 / +1
Query: 4 VCADLGAGAASYVHCDVTSEXXXXXXXXXXXXTFGALDIMFNNAGVSG 147
+ A LG SYVHCDV+ E G LD++ NNAGV G
Sbjct: 160 LAAALGGACCSYVHCDVSVEADVERAVGCCVARHGRLDVLCNNAGVLG 303
Score = 45.5 bits (93), Expect(4) = 2e-023
Identities = 19/32 (59%), Positives = 23/32 (71%)
Frame = +1 / +1
Query: 181 ADFERVLAVNLVGPFLGTKHAARVMVPSRRGG 276
A+F+RVL VN +G LG KHAAR M+ R GG
Sbjct: 364 AEFDRVLRVNTLGAALGMKHAARAMLQRRGGG 459
Score = 37.3 bits (75), Expect(4) = 2e-023
Identities = 13/19 (68%), Positives = 15/19 (78%)
Frame = +1 / +3
Query: 370 ENAAAELGQHGIRVNCVSP 426
+ ELG+HGIRVNCVSP
Sbjct: 564 QERGCELGEHGIRVNCVSP 620
Score = 36.3 bits (73), Expect(4) = 2e-023
Identities = 16/38 (42%), Positives = 18/38 (47%)
Frame = +3 / +3
Query: 270 RWLHHRDVERGGGSDWXXXXXXXXXEARDGDSDGERGC 383
RW H ERG + W EAR G +D ERGC
Sbjct: 465 RWDHRVGGERGRRARWDGPARVHGVEARAGGADQERGC 578
>gb|CD926685.1|CD926685 GR45.001M10F001019 GR45 Triticum aestivum cDNA clone GR45001M10,
mRNA sequence
Length = 718
Score = 89.0 bits (188), Expect(2) = 2e-022
Identities = 36/82 (43%), Positives = 48/82 (58%)
Frame = +1 / +2
Query: 37 YVHCDVTSEXXXXXXXXXXXXTFGALDIMFNNAGVSGPACYSVRESGKADFERVLAVNLV 216
+VHCDVT E FG LDIM NNAG++G +R AD ++V +N+
Sbjct: 308 FVHCDVTVEEDISRAVDATAEKFGTLDIMVNNAGITGDKVTDIRNLDFADVKKVFDINVH 487
Query: 217 GPFLGTKHAARVMVPSRRGGCI 282
G LG KHAARVM+PS++G +
Sbjct: 488 GMLLGMKHAARVMIPSKKGSIV 553
Score = 37.7 bits (76), Expect(2) = 2e-022
Identities = 17/35 (48%), Positives = 25/35 (71%)
Frame = +1 / +2
Query: 343 AKRAMVTLTENAAAELGQHGIRVNCVSPAGVATPL 447
+K A+V LT++ A EL ++GIR+NCV+ V T L
Sbjct: 611 SKHAVVGLTKSVALELLRYGIRLNCVTSYDVFTVL 715
>gb|BJ248602.1|BJ248602 BJ248602 Y. Ogihara unpublished cDNA library, Wh_f Triticum
aestivum cDNA clone whf7c11 5', mRNA sequence
Length = 609
Score = 62.5 bits (130), Expect(3) = 1e-021
Identities = 25/35 (71%), Positives = 30/35 (85%)
Frame = +1 / +2
Query: 343 AKRAMVTLTENAAAELGQHGIRVNCVSPAGVATPL 447
+K A+V LT+NAA ELG+HGIRVNCVSP GVAT +
Sbjct: 47 SKHALVGLTKNAACELGEHGIRVNCVSPFGVATSM 151
Score = 55.6 bits (115), Expect(3) = 1e-021
Identities = 22/40 (55%), Positives = 29/40 (72%)
Frame = +1 / +2
Query: 529 LRVHDVAAAVRFLASDDARYISGHNLFVDGGISVANPSTG 648
LR D+A A FLAS+++RYISGHNL VDGG++ + G
Sbjct: 287 LRAGDIAEAALFLASEESRYISGHNLVVDGGVTTSRNVIG 406
Score = 23.5 bits (45), Expect(3) = 1e-021
Identities = 9/17 (52%), Positives = 11/17 (64%)
Frame = +1 / +2
Query: 469 EAEALEQAMEGMANLKG 519
E E E+ + GMA LKG
Sbjct: 230 EVEKTEEMVRGMATLKG 280
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 806,087,596
Number of Sequences: 636343
Number of extensions: 12457666
Number of successful extensions: 230920
Number of sequences better than 1.0e-020: 10
length of database: 122,413,413
effective HSP length: 54
effective length of database: 88,050,891
effective search space used: 23245435224
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)