TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCN28g01.yg.2.1
(513 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG464188.1|BG464188 EM1_70_F11.b1_A002 Embryo 1 (EM1) So... 310 8e-084
gb|CN137529.1|CN137529 OX1_57_H05.g1_A002 Oxidatively-stres... 183 1e-057
gb|CF073501.1|CF073501 FE1_10_C03.g1_A002 Iron-deficient se... 157 1e-037
gb|CW295861.1|CW295861 104_777_11461177_148_35626_009 Sorgh... 84 3e-030
gb|CW118882.1|CW118882 104_495_11108451_148_34628_006 Sorgh... 104 1e-021
>gb|BG464188.1|BG464188 EM1_70_F11.b1_A002 Embryo 1 (EM1) Sorghum bicolor cDNA, mRNA
sequence
Length = 618
Score = 310 bits (672), Expect = 8e-084
Identities = 130/132 (98%), Positives = 130/132 (98%)
Frame = -1 / +2
Query: 495 LRLLLTVKESAMDVPTNLDARRRISFFANSLFMSMPDAPKVRQMLPFSVLTPYYKEDVLF 316
LRLLLTVKESAMDVPTNLDARRRISFFANSLFMSMPDAPKVRQMLPFSVLTPYYKEDVLF
Sbjct: 44 LRLLLTVKESAMDVPTNLDARRRISFFANSLFMSMPDAPKVRQMLPFSVLTPYYKEDVLF 223
Query: 315 SSQALAEQNEDGVSILFYLQKIYPDEWKNFLERVHCESEDQLHETEHSEEQLRLWASYRG 136
SSQAL EQNEDGVSILFYLQKIYPDEWKNFLERVHCESEDQLHETE SEEQLRLWASYRG
Sbjct: 224 SSQALGEQNEDGVSILFYLQKIYPDEWKNFLERVHCESEDQLHETEQSEEQLRLWASYRG 403
Query: 135 QTLTRTVRGMMY 100
QTLTRTVRGMMY
Sbjct: 404 QTLTRTVRGMMY 439
Score = 297 bits (642), Expect = 1e-079
Identities = 120/133 (90%), Positives = 125/133 (93%)
Frame = +1 / -3
Query: 97 LVHHPSYSSCQSLSPV*CPKAKLLFRMLSLVELIFTLTMHPFKKIFPFIWVDFLQIKKDG 276
+VHHP YSSCQSLSPV*CPKAKLLFR+L LVELIFTLTMHPFKKIFPFIWVDFLQI+KD
Sbjct: 442 VVHHPPYSSCQSLSPV*CPKAKLLFRLLGLVELIFTLTMHPFKKIFPFIWVDFLQIEKDR 263
Query: 277 NSVLILFC*CL*REKNIFFIIRSQDREGQHLPHFWSIWHRHKKRISKERYPPSSIQVCRD 456
N +LILF *CL*REKN+FFIIRSQDREGQHLPHFW IWHRHKKRISKERYPPSSIQVCRD
Sbjct: 262 NPILILFP*CL*REKNVFFIIRSQDREGQHLPHFWGIWHRHKKRISKERYPPSSIQVCRD 83
Query: 457 IHSRFLHRE*KSK 495
IHS FLHRE KSK
Sbjct: 82 IHS*FLHREQKSK 44
Score = 277 bits (600), Expect = 7e-074
Identities = 121/136 (88%), Positives = 122/136 (89%)
Frame = -2 / +3
Query: 494 FDFYSR*RNRLWMSLQTWMLEGGYLSLLILFLCRCQMLQKCGKCCPSLS*LLIIKKMFFS 315
FDF SR*RN+LWMSLQTWMLEGGYLSLLILFLCRCQM QKCGKCCPSLS*LLIIKK FFS
Sbjct: 45 FDFCSR*RNQLWMSLQTWMLEGGYLSLLILFLCRCQMPQKCGKCCPSLS*LLIIKKTFFS 224
Query: 314 LHKH*QNRMRTEFPSFFICKKSTQMNGKIFLKGCIVRVKINSTRLSILKSSFAFGHHTGD 135
LHKH* NRMR F SF ICKKSTQMNGKIFLKGCIVRVKINSTR S LKSSFAFGHHTGD
Sbjct: 225 LHKH*GNRMRMGFLSFSICKKSTQMNGKIFLKGCIVRVKINSTRPSNLKSSFAFGHHTGD 404
Query: 134 KL*QEL*EG*CTKKDL 87
KL*QEL* G*CT L
Sbjct: 405 KL*QEL*GG*CTTDKL 452
Score = 226 bits (488), Expect = 2e-058
Identities = 102/112 (91%), Positives = 104/112 (92%)
Frame = +3 / -2
Query: 102 TSSLLQFLSKFVPGMMPKGEAALQNAQSRGVDLHSHNAPFQENFSIHLGRFFANKKGWKL 281
TSS LQFLSKFVPGMMPKGEAALQ A+SRGVDLHSHNAPFQENFSIHLGRFFAN+KG K
Sbjct: 437 TSSPLQFLSKFVPGMMPKGEAALQIARSRGVDLHSHNAPFQENFSIHLGRFFANRKG*KP 258
Query: 282 RPHSVLLVLVKRKEHLLYNKESRQRRATFAALLEHLAST*KEN*QRKISSF* 437
PHSV LVLVKRKE LLYNKESRQRRATFAALL HLAST*KEN*QRKISSF*
Sbjct: 257 HPHSVPLVLVKRKERLLYNKESRQRRATFAALLGHLAST*KEN*QRKISSF* 102
Score = 198 bits (426), Expect(2) = 4e-059
Identities = 87/90 (96%), Positives = 89/90 (98%)
Frame = +2 / -1
Query: 224 RKFFHSSG*IFCK*KRMETPSSFCSASACEEKRTSSL**GVKTEKGNICRTFGASGIDIK 403
RKFFHSSG*IFCK*KR+ETPSSFCS SACEEKRTSSL**GVKTEKGNICRTFGASGIDIK
Sbjct: 315 RKFFHSSG*IFCK*KRIETPSSFCSPSACEEKRTSSL**GVKTEKGNICRTFGASGIDIK 136
Query: 404 RELAKKDILLLASRFVGTSIADSFTVSKSR 493
RELAKKDILLLASRFVGTSIADSFTVS+SR
Sbjct: 135 RELAKKDILLLASRFVGTSIADSFTVSRSR 46
Score = 135 bits (290), Expect(2) = 2e-056
Identities = 62/70 (88%), Positives = 63/70 (90%)
Frame = -3 / +1
Query: 310 TSTSRTE*GRSFHPFLFAKNLPR*MEKFS*KGAL*E*RSTPRD*AF*RAASPLGIIPGTN 131
TST TE*G F+PFLFAKNLPR*MEKFS*KGAL*E*RSTPRD A *RAASPLGIIPGTN
Sbjct: 229 TSTRGTE*GWGFYPFLFAKNLPR*MEKFS*KGAL*E*RSTPRDRAI*RAASPLGIIPGTN 408
Query: 130 FDKNCKRDDV 101
FDKNCK DDV
Sbjct: 409 FDKNCKGDDV 438
Score = 104 bits (222), Expect(2) = 2e-056
Identities = 41/46 (89%), Positives = 42/46 (91%)
Frame = -3 / +1
Query: 496 SSTFTHGEGIGYGCPYKPGC*KEDIFLC*FSFYVDARCSKSAANVA 359
+ST HGEGI YGCPYKPGC*KEDIFLC*FSFYVDARC KSAANVA
Sbjct: 43 ASTSAHGEGISYGCPYKPGC*KEDIFLC*FSFYVDARCPKSAANVA 180
Score = 51.0 bits (105), Expect(2) = 4e-059
Identities = 20/20 (100%), Positives = 20/20 (100%)
Frame = +2 / -1
Query: 101 YIIPLTVLVKVCPRYDAQRR 160
YIIPLTVLVKVCPRYDAQRR
Sbjct: 438 YIIPLTVLVKVCPRYDAQRR 379
>gb|CN137529.1|CN137529 OX1_57_H05.g1_A002 Oxidatively-stressed leaves and roots Sorghum
bicolor cDNA clone OX1_57_H05_A002 5', mRNA sequence
Length = 797
Score = 183 bits (395), Expect(2) = 1e-057
Identities = 77/99 (77%), Positives = 83/99 (83%)
Frame = -1 / +2
Query: 495 LRLLLTVKESAMDVPTNLDARRRISFFANSLFMSMPDAPKVRQMLPFSVLTPYYKEDVLF 316
L LLLTVKESAMDVP+NL+ARRR++FF NSLFM MPDAPKVR ML FS LTPYY E VLF
Sbjct: 287 LELLLTVKESAMDVPSNLEARRRLTFFTNSLFMDMPDAPKVRNMLSFSALTPYYNEPVLF 466
Query: 315 SSQALAEQNEDGVSILFYLQKIYPDEWKNFLERVHCESE 199
S + L E+NEDGVS LFYLQKIYPDEWKNF ERV E E
Sbjct: 467 SIKELEEENEDGVSTLFYLQKIYPDEWKNFKERVGLEEE 583
Score = 130 bits (278), Expect(2) = 1e-032
Identities = 62/88 (70%), Positives = 69/88 (78%)
Frame = +2 / -3
Query: 227 KFFHSSG*IFCK*KRMETPSSFCSASACEEKRTSSL**GVKTEKGNICRTFGASGIDIKR 406
KFFHSSG*IFC+* R+ETPSSF S+S+ EKRT SL**GV E ++ RT GASGI I +
Sbjct: 555 KFFHSSG*IFCR*NRVETPSSFSSSSSFIEKRTGSL**GVNAENESMFRTLGASGISINK 376
Query: 407 ELAKKDILLLASRFVGTSIADSFTVSKS 490
EL KK LLASRF GTSIADSFTVS S
Sbjct: 375 ELVKKVRRLLASRFEGTSIADSFTVSSS 292
Score = 60.6 bits (126), Expect(2) = 1e-057
Identities = 24/39 (61%), Positives = 30/39 (76%)
Frame = -1 / +2
Query: 207 ESEDQLHETEHSEEQLRLWASYRGQTLTRTVRGMMYQER 91
E + E+E +E+LRLWASYRGQTL RTVRGMMY ++
Sbjct: 578 EELKESEESEELKEELRLWASYRGQTLARTVRGMMYYKK 694
Score = 30.4 bits (60), Expect(2) = 1e-032
Identities = 12/20 (60%), Positives = 14/20 (70%)
Frame = +2 / -3
Query: 101 YIIPLTVLVKVCPRYDAQRR 160
YI+PLTV V P Y+A RR
Sbjct: 684 YIMPLTVRASVWPLYEAHRR 625
>gb|CF073501.1|CF073501 FE1_10_C03.g1_A002 Iron-deficient seedlings Sorghum bicolor cDNA
clone FE1_10_C03_A002 5', mRNA sequence
Length = 782
Score = 157 bits (337), Expect = 1e-037
Identities = 66/84 (78%), Positives = 72/84 (85%)
Frame = -1 / +2
Query: 495 LRLLLTVKESAMDVPTNLDARRRISFFANSLFMSMPDAPKVRQMLPFSVLTPYYKEDVLF 316
L LLLTVKESAMDVP+NL+ARRR++FF NSLFM MPDAPKVR ML FS LTPYY E VLF
Sbjct: 359 LELLLTVKESAMDVPSNLEARRRLTFFTNSLFMDMPDAPKVRNMLSFSALTPYYNEPVLF 538
Query: 315 SSQALAEQNEDGVSILFYLQKIYP 244
S + L E+NEDGVS LFYLQKIYP
Sbjct: 539 SIKELEEENEDGVSTLFYLQKIYP 610
Score = 115 bits (245), Expect = 6e-025
Identities = 56/82 (68%), Positives = 63/82 (76%)
Frame = +2 / -3
Query: 245 G*IFCK*KRMETPSSFCSASACEEKRTSSL**GVKTEKGNICRTFGASGIDIKRELAKKD 424
G*IFC+* R+ETPSSF S+S+ EKRT SL**GV E ++ RT GASGI I +EL KK
Sbjct: 609 G*IFCR*NRVETPSSFSSSSSFIEKRTGSL**GVNAENESMFRTLGASGISINKELVKKV 430
Query: 425 ILLLASRFVGTSIADSFTVSKS 490
LLASRF GTSIADSFTVS S
Sbjct: 429 RRLLASRFEGTSIADSFTVSSS 364
>gb|CW295861.1|CW295861 104_777_11461177_148_35626_009 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11461177, DNA
sequence
Length = 678
Score = 83.5 bits (176), Expect(2) = 3e-030
Identities = 36/50 (72%), Positives = 41/50 (82%)
Frame = -1 / -1
Query: 504 C*ALRLLLTVKESAMDVPTNLDARRRISFFANSLFMSMPDAPKVRQMLPF 355
C L+LLLT KESA+ VPTNL+ARRRI+FFANSLFM MP AP VR M+ F
Sbjct: 597 CIRLQLLLTTKESAIYVPTNLEARRRITFFANSLFMKMPRAPPVRSMMSF 448
Score = 70.3 bits (147), Expect(2) = 3e-030
Identities = 27/37 (72%), Positives = 34/37 (91%)
Frame = -1 / -1
Query: 354 SVLTPYYKEDVLFSSQALAEQNEDGVSILFYLQKIYP 244
SVLTPY+KE+VLFS + L ++NEDG+SILFYL+KIYP
Sbjct: 207 SVLTPYFKEEVLFSPEDLRKKNEDGISILFYLRKIYP 97
>gb|CW118882.1|CW118882 104_495_11108451_148_34628_006 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11108451, DNA
sequence
Length = 646
Score = 104 bits (221), Expect = 1e-021
Identities = 46/54 (85%), Positives = 47/54 (87%)
Frame = -1 / +1
Query: 513 FFFC*ALRLLLTVKESAMDVPTNLDARRRISFFANSLFMSMPDAPKVRQMLPFS 352
FF L LLLTVKESAMDVPTNLDARRRISFFANSLFM MP+APKVR MLPFS
Sbjct: 286 FFQIKRLHLLLTVKESAMDVPTNLDARRRISFFANSLFMDMPNAPKVRNMLPFS 447
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 599,136,228
Number of Sequences: 832831
Number of extensions: 8111674
Number of successful extensions: 92998
Number of sequences better than 1.0e-020: 5
length of database: 163,786,556
effective HSP length: 53
effective length of database: 119,646,513
effective search space used: 13998642021
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)