TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCM12h10.yg.2.1
(339 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CD219775.1|CD219775 CCC1_58_A07.g1_A007 Callus culture/c... 187 7e-047
gb|CD428230.1|CD428230 ETH1_28_F10.g1_A002 Ethylene-treated... 172 2e-042
gb|BG355549.1|BG355549 EM1_17_G07.b1_A002 Embryo 1 (EM1) So... 151 4e-036
gb|BI643696.1|BI643696 EM1_19_F12.b1_A002 Embryo 1 (EM1) So... 151 4e-036
gb|CW414651.1|CW414651 fsbb001f114g15k0 Sorghum methylation... 146 1e-034
gb|BM325000.1|BM325000 PIC1_38_E07.b1_A002 Pathogen-infecte... 145 3e-034
gb|CW256112.1|CW256112 104_721_11226607_148_35144_032 Sorgh... 144 7e-034
gb|CL170431.1|CL170431 104_371_10813804_148_31790_124 Sorgh... 137 8e-032
gb|CW173113.1|CW173113 104_584_11156668_148_36547_006 Sorgh... 127 4e-029
gb|CF482724.1|CF482724 POL1_8_F04.g1_A002 Pollen Sorghum bi... 113 2e-027
gb|CN147848.1|CN147848 WOUND1_52_D08.g1_A002 Wounded leaves... 100 6e-021
>gb|CD219775.1|CD219775 CCC1_58_A07.g1_A007 Callus culture/cell suspension Sorghum bicolor
cDNA clone CCC1_58_A07_A007 5', mRNA sequence
Length = 669
Score = 187 bits (402), Expect = 7e-047
Identities = 77/110 (70%), Positives = 86/110 (78%)
Frame = -3 / +3
Query: 337 FSSVXXTFSMGTKSXXXDXXILHGGAKYRATXRGFVVQHKSFAENYXLYARGHFIKAIEL 158
F+SV TFSMGTK+ ILHGGAKYRAT RGFVV+HK FAENY LYAR HFIKAIEL
Sbjct: 300 FASVFYTFSMGTKTHYYGRTILHGGAKYRATGRGFVVEHKKFAENYRLYARSHFIKAIEL 479
Query: 157 GIILTVYAAHSVIAKNTLVYIXXXXSSWFLVVSWIMAPFAFXXSGFDWLK 8
G+ILT+YA++ + NTLVYI SSWFLV SWI+APF F SGFDWLK
Sbjct: 480 GVILTLYASYGSASGNTLVYILLTLSSWFLVSSWILAPFIFNPSGFDWLK 629
Score = 90.0 bits (190), Expect(2) = 2e-025
Identities = 44/66 (66%), Positives = 45/66 (68%)
Frame = +3 / -3
Query: 135 AYTVNIIPSSIAFMKWPLAYSX*FSAKLLCCTTKPRXVARYFAPP*RIXXSXXWLLVPME 314
AY VNI PSSIA MKW A S *FSA LC TTKP VARYFAPP*RI + VPME
Sbjct: 502 AYRVNITPSSIALMKWLRA*SL*FSANFLCSTTKPLPVARYFAPP*RIVLP**CVFVPME 323
Query: 315 NVXNTD 332
NV TD
Sbjct: 322 NV*KTD 305
Score = 44.6 bits (91), Expect(2) = 2e-025
Identities = 20/35 (57%), Positives = 22/35 (62%)
Frame = +3 / -3
Query: 9 FNQSKPXXXKANGAIIHDTTRNQLXXXXXM*TSVF 113
F+QSKP K GA IHD TRNQL + TSVF
Sbjct: 628 FSQSKPEGLKMKGARIHDDTRNQLESVSRIYTSVF 524
>gb|CD428230.1|CD428230 ETH1_28_F10.g1_A002 Ethylene-treated seedlings Sorghum bicolor cDNA
clone ETH1_28_F10_A002 5', mRNA sequence
Length = 533
Score = 172 bits (370), Expect = 2e-042
Identities = 68/78 (87%), Positives = 69/78 (88%)
Frame = +2 / -1
Query: 2 HCFQPIKTRXXKSKWSHNP*YHQKPARXXXXYVNKCILGNNTVCSIHSQYYSKLYCLYEV 181
HCFQP KTR KSKWSHNP*YHQKPAR YVNKCILGNNTVCSIHSQYYSK YCLYEV
Sbjct: 242 HCFQPTKTRWIKSKWSHNP*YHQKPARYIHDYVNKCILGNNTVCSIHSQYYSKFYCLYEV 63
Query: 182 APSIQXVIFSKTLVLHNK 235
APSIQ VIFSK+LVLHNK
Sbjct: 62 APSIQPVIFSKSLVLHNK 9
Score = 160 bits (344), Expect = 7e-039
Identities = 68/78 (87%), Positives = 68/78 (87%)
Frame = -3 / +3
Query: 235 FVVQHKSFAENYXLYARGHFIKAIELGIILTVYAAHSVIAKNTLVYIXXXXSSWFLVVSW 56
FVVQHK FAENY LYAR HFIKAIELGIILTVYAAHSVIAKNTLVYI SSWFLVVSW
Sbjct: 9 FVVQHKRFAENYRLYARSHFIKAIELGIILTVYAAHSVIAKNTLVYIIMNISSWFLVVSW 188
Query: 55 IMAPFAFXXSGFDWLKTV 2
IMAPFAF SGF WLKTV
Sbjct: 189 IMAPFAFNPSGFGWLKTV 242
Score = 155 bits (332), Expect = 3e-037
Identities = 68/78 (87%), Positives = 68/78 (87%)
Frame = +1 / -3
Query: 1 TLFSTNQNQXX*KQMEP*SMIPPETS*XXXXXCKQVYSWQ*HCVQHTQSILFQALLPL*S 180
TLFSTNQNQ *KQMEP*SMIPPETS* CKQVYSWQ*HCVQHTQSILFQ LLPL*S
Sbjct: 243 TLFSTNQNQMD*KQMEP*SMIPPETS*IYS*LCKQVYSWQ*HCVQHTQSILFQVLLPL*S 64
Query: 181 GP*HTACNFQQNSCAAQQ 234
G *HTACNFQQ SCAAQQ
Sbjct: 63 GS*HTACNFQQISCAAQQ 10
Score = 147 bits (315), Expect = 7e-035
Identities = 65/77 (84%), Positives = 65/77 (84%)
Frame = +3 / -2
Query: 3 TVFNQSKPXXXKANGAIIHDTTRNQLXXXXXM*TSVFLAITLCAAYTVNIIPSSIAFMKW 182
TVFNQ KP KANGAIIHDTTRNQL M*TSVFLAITLCAAYTVNIIPSSIAFMKW
Sbjct: 241 TVFNQPKPDGLKANGAIIHDTTRNQLDIFMIM*TSVFLAITLCAAYTVNIIPSSIAFMKW 62
Query: 183 PLAYSX*FSAKLLCCTT 233
LAYS *FSA LLCCTT
Sbjct: 61 LLAYSL*FSANLLCCTT 11
Score = 111 bits (236), Expect = 6e-024
Identities = 50/69 (72%), Positives = 52/69 (75%)
Frame = -1 / +1
Query: 207 KITXCMLGATS*RQ*SLE*Y*LCMLHTVLLPRIHLFTXXXXXLAGFWWYHGXXXXXXXXX 28
KIT CMLGATS*RQ*+LE*Y*LCMLHTVLLPRIHLFT LAGFWWYHG
Sbjct: 37 KITGCMLGATS*RQ*NLE*Y*LCMLHTVLLPRIHLFT*S*IYLAGFWWYHGLWLHLLLIH 216
Query: 27 XXLIG*KQC 1
L+G*KQC
Sbjct: 217 LVLVG*KQC 243
Score = 68.9 bits (144), Expect(2) = 2e-022
Identities = 26/30 (86%), Positives = 26/30 (86%)
Frame = -2 / +2
Query: 233 CCAAQEFC*KLXAVC*GPLHKGNRAWNNID 144
CCAAQE C*KL AVC* PLHKGNR WNNID
Sbjct: 11 CCAAQEIC*KLQAVC*EPLHKGNRTWNNID 100
Score = 55.1 bits (114), Expect(2) = 2e-022
Identities = 24/33 (72%), Positives = 24/33 (72%)
Frame = -2 / +2
Query: 101 LHXXXXX*LVSGGIMDYGSICFXXXWF*LVENS 3
LH *LVSGGIMDYGSICF WF LVENS
Sbjct: 143 LHNHEYI*LVSGGIMDYGSICF*SIWFWLVENS 241
>gb|BG355549.1|BG355549 EM1_17_G07.b1_A002 Embryo 1 (EM1) Sorghum bicolor cDNA, mRNA
sequence
Length = 502
Score = 151 bits (324), Expect = 4e-036
Identities = 59/112 (52%), Positives = 78/112 (69%)
Frame = -3 / +2
Query: 337 FSSVXXTFSMGTKSXXXDXXILHGGAKYRATXRGFVVQHKSFAENYXLYARGHFIKAIEL 158
F SV TFS+GT++ ILHGGAKY+AT RGFVV+H FAENY LY+R HF+KA+E+
Sbjct: 29 FCSVFFTFSLGTRTHYFGRTILHGGAKYKATGRGFVVRHIKFAENYRLYSRSHFVKALEV 208
Query: 157 GIILTVYAAHSVIAKNTLVYIXXXXSSWFLVVSWIMAPFAFXXSGFDWLKTV 2
++L VY ++ + +I SSWFLV+SW+ AP+ F SGF+W KTV
Sbjct: 209 ALLLIVYISYGYTKGGSSSFILITISSWFLVMSWLFAPYIFNPSGFEWQKTV 364
>gb|BI643696.1|BI643696 EM1_19_F12.b1_A002 Embryo 1 (EM1) Sorghum bicolor cDNA, mRNA
sequence
Length = 631
Score = 151 bits (324), Expect = 4e-036
Identities = 59/112 (52%), Positives = 78/112 (69%)
Frame = -3 / +2
Query: 337 FSSVXXTFSMGTKSXXXDXXILHGGAKYRATXRGFVVQHKSFAENYXLYARGHFIKAIEL 158
F SV TFS+GT++ ILHGGAKY+AT RGFVV+H FAENY LY+R HF+KA+E+
Sbjct: 29 FCSVFFTFSLGTRTHYFGRTILHGGAKYKATGRGFVVRHIKFAENYRLYSRSHFVKALEV 208
Query: 157 GIILTVYAAHSVIAKNTLVYIXXXXSSWFLVVSWIMAPFAFXXSGFDWLKTV 2
++L VY ++ + +I SSWFLV+SW+ AP+ F SGF+W KTV
Sbjct: 209 ALLLIVYISYGYTKGGSSSFILITISSWFLVMSWLFAPYIFNPSGFEWQKTV 364
>gb|CW414651.1|CW414651 fsbb001f114g15k0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f114g15, DNA
sequence
Length = 636
Score = 146 bits (314), Expect = 1e-034
Identities = 59/111 (53%), Positives = 73/111 (65%)
Frame = -3 / +2
Query: 334 SSVXXTFSMGTKSXXXDXXILHGGAKYRATXRGFVVQHKSFAENYXLYARGHFIKAIELG 155
+SV TFS+GTK+ +LHGGA+YRAT RGFVV H FAENY LY+R HF+K IEL
Sbjct: 32 ASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHFVKGIELM 211
Query: 154 IILTVYAAHSVIAKNTLVYIXXXXSSWFLVVSWIMAPFAFXXSGFDWLKTV 2
I+L VY + + YI S WF+VV+W+ APF F SGF+W K V
Sbjct: 212 ILLIVYEIFGQSYRGAIAYIFITISMWFMVVTWLFAPFLFNPSGFEWQKIV 364
>gb|BM325000.1|BM325000 PIC1_38_E07.b1_A002 Pathogen-infected compatible 1 (PIC1) Sorghum
bicolor cDNA, mRNA sequence
Length = 558
Score = 145 bits (311), Expect = 3e-034
Identities = 58/111 (52%), Positives = 74/111 (66%)
Frame = -3 / +3
Query: 334 SSVXXTFSMGTKSXXXDXXILHGGAKYRATXRGFVVQHKSFAENYXLYARGHFIKAIELG 155
++V TFS+GTK+ +LHGGA+YR T RGFVV H FAENY LY+R HF+K IEL
Sbjct: 189 ATVFFTFSLGTKTHYYGRMLLHGGAQYRGTGRGFVVFHAKFAENYRLYSRSHFVKGIELM 368
Query: 154 IILTVYAAHSVIAKNTLVYIXXXXSSWFLVVSWIMAPFAFXXSGFDWLKTV 2
I+L VY ++T+ YI S WFLV++W+ APF F SGF+W K V
Sbjct: 369 ILLIVYQLFGQSYRSTIAYIFITISMWFLVLTWLFAPFLFNPSGFEWAKIV 521
>gb|CW256112.1|CW256112 104_721_11226607_148_35144_032 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11226607, DNA
sequence
Length = 678
Score = 144 bits (308), Expect = 7e-034
Identities = 58/109 (53%), Positives = 74/109 (67%)
Frame = -3 / +1
Query: 328 VXXTFSMGTKSXXXDXXILHGGAKYRATXRGFVVQHKSFAENYXLYARGHFIKAIELGII 149
V TF +GTK+ ILHGGAKYRAT RGFVV+H FAENY +Y+R HF+KA+EL I+
Sbjct: 115 VFFTFHLGTKTHYYGRTILHGGAKYRATGRGFVVRHAKFAENYRMYSRSHFVKALELLIL 294
Query: 148 LTVYAAHSVIAKNTLVYIXXXXSSWFLVVSWIMAPFAFXXSGFDWLKTV 2
L VY A+ +++ +Y+ S WFLV W+ APF F S F+W KTV
Sbjct: 295 LVVYLAYGSSYRSSSLYLYVTVSIWFLVFCWLFAPFIFNPSCFEWHKTV 441
>gb|CL170431.1|CL170431 104_371_10813804_148_31790_124 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10813804, DNA
sequence
Length = 656
Score = 137 bits (293), Expect = 8e-032
Identities = 58/86 (67%), Positives = 66/86 (76%)
Frame = -3 / -2
Query: 337 FSSVXXTFSMGTKSXXXDXXILHGGAKYRATXRGFVVQHKSFAENYXLYARGHFIKAIEL 158
F+SV TFSMGTK+ ILHGGAKYRAT RGFVV+HK FAENY LYAR HFIKAIEL
Sbjct: 259 FASVFYTFSMGTKTHYYGRTILHGGAKYRATGRGFVVEHKKFAENYRLYARSHFIKAIEL 80
Query: 157 GIILTVYAAHSVIAKNTLVYIXXXXS 80
G+ILT+YA++ + NTLVYI S
Sbjct: 79 GVILTLYASYGSASGNTLVYILLTLS 2
>gb|CW173113.1|CW173113 104_584_11156668_148_36547_006 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11156668, DNA
sequence
Length = 628
Score = 127 bits (273), Expect = 4e-029
Identities = 50/92 (54%), Positives = 63/92 (68%)
Frame = -3 / +3
Query: 277 ILHGGAKYRATXRGFVVQHKSFAENYXLYARGHFIKAIELGIILTVYAAHSVIAKNTLVY 98
ILHGGAKYR T RGFVV H FAENY +Y+R HF+K +EL I+L VY + +++ +Y
Sbjct: 18 ILHGGAKYRPTGRGFVVYHAKFAENYRMYSRSHFVKGLELLILLVVYLVYGRSYRSSSLY 197
Query: 97 IXXXXSSWFLVVSWIMAPFAFXXSGFDWLKTV 2
+ S WFLV SW+ APF F S F+W KTV
Sbjct: 198 LFVTCSMWFLVASWLFAPFIFNPSCFEWQKTV 293
>gb|CF482724.1|CF482724 POL1_8_F04.g1_A002 Pollen Sorghum bicolor cDNA clone
POL1_8_F04_A002 5', mRNA sequence
Length = 773
Score = 113 bits (241), Expect(2) = 2e-027
Identities = 43/83 (51%), Positives = 58/83 (69%)
Frame = -3 / +2
Query: 250 ATXRGFVVQHKSFAENYXLYARGHFIKAIELGIILTVYAAHSVIAKNTLVYIXXXXSSWF 71
AT RGFVV+H FAENY LY+R HF+KA+E+ ++L VY ++ + +I SSWF
Sbjct: 41 ATGRGFVVRHIKFAENYRLYSRSHFVKALEVALLLIVYISYGYTKGGSSSFILITISSWF 220
Query: 70 LVVSWIMAPFAFXXSGFDWLKTV 2
LV+SW+ AP+ F SGF+W KTV
Sbjct: 221 LVMSWLFAPYIFNPSGFEWQKTV 289
Score = 28.6 bits (56), Expect(2) = 2e-027
Identities = 7/8 (87%), Positives = 8/8 (100%)
Frame = -2 / +2
Query: 278 NPSWWCKI 255
NPSWWCK+
Sbjct: 14 NPSWWCKV 37
>gb|CN147848.1|CN147848 WOUND1_52_D08.g1_A002 Wounded leaves Sorghum bicolor cDNA clone
WOUND1_52_D08_A002 5', mRNA sequence
Length = 731
Score = 100 bits (214), Expect = 6e-021
Identities = 36/80 (45%), Positives = 52/80 (65%)
Frame = -3 / +2
Query: 241 RGFVVQHKSFAENYXLYARGHFIKAIELGIILTVYAAHSVIAKNTLVYIXXXXSSWFLVV 62
RGFVV+H FAENY +YAR HF+K +E+ ++L ++ + + YI SSWF+ +
Sbjct: 2 RGFVVRHIKFAENYRIYARSHFVKGMEVALLLVIFLVYGFNNGGAVGYILLSISSWFMAL 181
Query: 61 SWIMAPFAFXXSGFDWLKTV 2
SW+ AP+ F SGF+W K V
Sbjct: 182 SWLFAPYLFNPSGFEWQKIV 241
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 340,515,112
Number of Sequences: 832831
Number of extensions: 3479265
Number of successful extensions: 177494
Number of sequences better than 1.0e-020: 12
length of database: 163,786,556
effective HSP length: 51
effective length of database: 121,312,175
effective search space used: 7400042675
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)