TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCL28h07.yg.2.1
(277 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CW414651.1|CW414651 fsbb001f114g15k0 Sorghum methylation... 202 1e-051
gb|BM325000.1|BM325000 PIC1_38_E07.b1_A002 Pathogen-infecte... 182 2e-045
gb|CW256112.1|CW256112 104_721_11226607_148_35144_032 Sorgh... 163 5e-040
gb|BG355549.1|BG355549 EM1_17_G07.b1_A002 Embryo 1 (EM1) So... 146 7e-035
gb|BI643696.1|BI643696 EM1_19_F12.b1_A002 Embryo 1 (EM1) So... 146 7e-035
gb|CL170431.1|CL170431 104_371_10813804_148_31790_124 Sorgh... 145 2e-034
gb|CD219775.1|CD219775 CCC1_58_A07.g1_A007 Callus culture/c... 145 2e-034
gb|BF657000.1|BF657000 OV2_19_G06.b1_A002 Ovary 2 (OV2) Sor... 132 2e-030
gb|CW173113.1|CW173113 104_584_11156668_148_36547_006 Sorgh... 127 4e-029
>gb|CW414651.1|CW414651 fsbb001f114g15k0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f114g15, DNA
sequence
Length = 636
Score = 202 bits (436), Expect = 1e-051
Identities = 85/85 (100%), Positives = 85/85 (100%)
Frame = -1 / +2
Query: 256 VLMQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHF 77
VLMQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHF
Sbjct: 11 VLMQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHF 190
Query: 76 VKGIELMILLIVYEIFGQSYRGAIA 2
VKGIELMILLIVYEIFGQSYRGAIA
Sbjct: 191 VKGIELMILLIVYEIFGQSYRGAIA 265
Score = 186 bits (400), Expect = 9e-047
Identities = 72/80 (90%), Positives = 75/80 (93%)
Frame = -3 / +1
Query: 242 PVSICVLHILSWDQNSLLRNHAATWRS*I*SYRAWICGVPCQVC*ELSIVFAKPFCEGH* 63
PVSICVLHILSWDQNSLLRNH TWRS*I*SYRAWICG+PCQ+C*ELSIVF K FCEGH
Sbjct: 25 PVSICVLHILSWDQNSLLRNHTVTWRS*I*SYRAWICGLPCQIC*ELSIVFTKSFCEGHR 204
Query: 62 TDDFAYCV*NIWAVIPRSYC 3
TDDFA+CV*NIWAVIPRSYC
Sbjct: 205 TDDFAHCV*NIWAVIPRSYC 264
Score = 174 bits (375), Expect = 3e-043
Identities = 73/81 (90%), Positives = 75/81 (92%)
Frame = +1 / -2
Query: 1 TQ*LLGMTAQIFHTQ*AKSSVQCPSQNGFANTIDNSQQTWHGTPQIHAL*LYIQLLHVAA 180
TQ*LLGMTAQIFHTQ*AKSSV+CPSQN F NTID+SQQ WHG PQIHAL*LYIQLLHV
Sbjct: 266 TQ*LLGMTAQIFHTQ*AKSSVRCPSQNDFVNTIDSSQQIWHGRPQIHAL*LYIQLLHVTV 87
Query: 181 WFRSSEFWSQERM*RTQMLTG 243
WFRSSEFWSQERM*RTQMLTG
Sbjct: 86 WFRSSEFWSQERM*RTQMLTG 24
Score = 145 bits (312), Expect = 1e-034
Identities = 61/69 (88%), Positives = 62/69 (89%)
Frame = -2 / +3
Query: 207 GPKLTTTEPRCYMEELNIELQGVDLWCSMPSLLRIIDCIREAIL*RALN**FCLLCMKYL 28
GPKLTTTEP CYMEELNIELQGVDLW SMP+LLR IDCI E IL*RA N**FC LCMKYL
Sbjct: 60 GPKLTTTEPHCYMEELNIELQGVDLWSSMPNLLRTIDCIHEVIL*RASN**FCSLCMKYL 239
Query: 27 GSHTEELLR 1
GSHTEELLR
Sbjct: 240 GSHTEELLR 266
Score = 141 bits (302), Expect(2) = 2e-043
Identities = 61/69 (88%), Positives = 64/69 (92%)
Frame = +2 / -3
Query: 2 RNSSSV*LPKYFIHNKQNHQFNALHKMASRIQSIILSKLGMEHHKSTPCSSIFSSSM*QR 181
RNSSSV*LPKYFIHN+QNHQF+ALHKM S IQSI+LSK GME HKSTPCSSIFSSSM*Q
Sbjct: 265 RNSSSV*LPKYFIHNEQNHQFDALHKMTS*IQSIVLSKFGMEDHKSTPCSSIFSSSM*QC 86
Query: 182 GSVVVSFGP 208
GSVVVSFGP
Sbjct: 85 GSVVVSFGP 59
Score = 119 bits (255), Expect = 9e-027
Identities = 49/52 (94%), Positives = 50/52 (96%)
Frame = +3 / -1
Query: 3 AIAPRYDCPNISYTISKIISSMPFTKWLREYNR*FSANLAWNTTNPRPVALY 158
AIAPRYDCPNISYT+SKIISSMPFTK LREYNR*FSANLAW TTNPRPVALY
Sbjct: 264 AIAPRYDCPNISYTMSKIISSMPFTK*LREYNR*FSANLAWKTTNPRPVALY 109
Score = 52.8 bits (109), Expect(2) = 2e-043
Identities = 21/21 (100%), Positives = 21/21 (100%)
Frame = +3 / -1
Query: 195 *VLVPRENVKNTDANWSCIST 257
*VLVPRENVKNTDANWSCIST
Sbjct: 72 *VLVPRENVKNTDANWSCIST 10
>gb|BM325000.1|BM325000 PIC1_38_E07.b1_A002 Pathogen-infected compatible 1 (PIC1) Sorghum
bicolor cDNA, mRNA sequence
Length = 558
Score = 182 bits (391), Expect = 2e-045
Identities = 73/85 (85%), Positives = 79/85 (92%)
Frame = -1 / +3
Query: 256 VLMQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHF 77
++M LQLA+VFFTFSLGTKTHYYG LLHGGA+YR TGRGFVVFHAKFAENYRLYSRSHF
Sbjct: 168 IMMNLQLATVFFTFSLGTKTHYYGRMLLHGGAQYRGTGRGFVVFHAKFAENYRLYSRSHF 347
Query: 76 VKGIELMILLIVYEIFGQSYRGAIA 2
VKGIELMILLIVY++FGQSYR IA
Sbjct: 348 VKGIELMILLIVYQLFGQSYRSTIA 422
>gb|CW256112.1|CW256112 104_721_11226607_148_35144_032 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11226607, DNA
sequence
Length = 678
Score = 163 bits (351), Expect = 5e-040
Identities = 65/83 (78%), Positives = 74/83 (89%)
Frame = -1 / +1
Query: 256 VLMQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHF 77
V+MQLQLA VFFTF LGTKTHYYG T+LHGGA+YRATGRGFVV HAKFAENYR+YSRSHF
Sbjct: 88 VIMQLQLAPVFFTFHLGTKTHYYGRTILHGGAKYRATGRGFVVRHAKFAENYRMYSRSHF 267
Query: 76 VKGIELMILLIVYEIFGQSYRGA 8
VK +EL+ILL+VY +G SYR +
Sbjct: 268 VKALELLILLVVYLAYGSSYRSS 336
>gb|BG355549.1|BG355549 EM1_17_G07.b1_A002 Embryo 1 (EM1) Sorghum bicolor cDNA, mRNA
sequence
Length = 502
Score = 146 bits (314), Expect = 7e-035
Identities = 58/83 (69%), Positives = 70/83 (84%)
Frame = -1 / +2
Query: 256 VLMQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHF 77
+ MQLQ SVFFTFSLGT+THY+G T+LHGGA+Y+ATGRGFVV H KFAENYRLYSRSHF
Sbjct: 11 ITMQLQFCSVFFTFSLGTRTHYFGRTILHGGAKYKATGRGFVVRHIKFAENYRLYSRSHF 190
Query: 76 VKGIELMILLIVYEIFGQSYRGA 8
VK +E+ +LLIVY +G + G+
Sbjct: 191 VKALEVALLLIVYISYGYTKGGS 259
>gb|BI643696.1|BI643696 EM1_19_F12.b1_A002 Embryo 1 (EM1) Sorghum bicolor cDNA, mRNA
sequence
Length = 631
Score = 146 bits (314), Expect = 7e-035
Identities = 58/83 (69%), Positives = 70/83 (84%)
Frame = -1 / +2
Query: 256 VLMQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHF 77
+ MQLQ SVFFTFSLGT+THY+G T+LHGGA+Y+ATGRGFVV H KFAENYRLYSRSHF
Sbjct: 11 ITMQLQFCSVFFTFSLGTRTHYFGRTILHGGAKYKATGRGFVVRHIKFAENYRLYSRSHF 190
Query: 76 VKGIELMILLIVYEIFGQSYRGA 8
VK +E+ +LLIVY +G + G+
Sbjct: 191 VKALEVALLLIVYISYGYTKGGS 259
>gb|CL170431.1|CL170431 104_371_10813804_148_31790_124 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10813804, DNA
sequence
Length = 656
Score = 145 bits (311), Expect = 2e-034
Identities = 56/77 (72%), Positives = 67/77 (87%)
Frame = -1 / -2
Query: 250 MQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHFVK 71
MQLQ ASVF+TFS+GTKTHYYG T+LHGGA+YRATGRGFVV H KFAENYRLY+RSHF+K
Sbjct: 271 MQLQFASVFYTFSMGTKTHYYGRTILHGGAKYRATGRGFVVEHKKFAENYRLYARSHFIK 92
Query: 70 GIELMILLIVYEIFGQS 20
IEL ++L +Y +G +
Sbjct: 91 AIELGVILTLYASYGSA 41
>gb|CD219775.1|CD219775 CCC1_58_A07.g1_A007 Callus culture/cell suspension Sorghum bicolor
cDNA clone CCC1_58_A07_A007 5', mRNA sequence
Length = 669
Score = 145 bits (311), Expect = 2e-034
Identities = 56/77 (72%), Positives = 67/77 (87%)
Frame = -1 / +3
Query: 250 MQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHFVK 71
MQLQ ASVF+TFS+GTKTHYYG T+LHGGA+YRATGRGFVV H KFAENYRLY+RSHF+K
Sbjct: 288 MQLQFASVFYTFSMGTKTHYYGRTILHGGAKYRATGRGFVVEHKKFAENYRLYARSHFIK 467
Query: 70 GIELMILLIVYEIFGQS 20
IEL ++L +Y +G +
Sbjct: 468 AIELGVILTLYASYGSA 518
>gb|BF657000.1|BF657000 OV2_19_G06.b1_A002 Ovary 2 (OV2) Sorghum bicolor cDNA, mRNA
sequence
Length = 455
Score = 132 bits (282), Expect = 2e-030
Identities = 51/65 (78%), Positives = 58/65 (89%)
Frame = -1 / +2
Query: 256 VLMQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHF 77
+ MQLQ SVFFTFSLGT+THY+G T+LHGGA+Y+ATGRGFVV H KFAENYRLYSRSHF
Sbjct: 260 ITMQLQFCSVFFTFSLGTRTHYFGRTILHGGAKYKATGRGFVVRHIKFAENYRLYSRSHF 439
Query: 76 VKGIE 62
VK +E
Sbjct: 440 VKALE 454
>gb|CW173113.1|CW173113 104_584_11156668_148_36547_006 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11156668, DNA
sequence
Length = 628
Score = 127 bits (272), Expect = 4e-029
Identities = 47/62 (75%), Positives = 58/62 (93%)
Frame = -1 / +3
Query: 193 YYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHFVKGIELMILLIVYEIFGQSYR 14
YYG T+LHGGA+YR TGRGFVV+HAKFAENYR+YSRSHFVKG+EL+ILL+VY ++G+SYR
Sbjct: 3 YYGRTILHGGAKYRPTGRGFVVYHAKFAENYRMYSRSHFVKGLELLILLVVYLVYGRSYR 182
Query: 13 GA 8
+
Sbjct: 183 SS 188
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 328,180,357
Number of Sequences: 832831
Number of extensions: 3834992
Number of successful extensions: 307210
Number of sequences better than 1.0e-020: 9
length of database: 163,786,556
effective HSP length: 50
effective length of database: 122,145,006
effective search space used: 5007945246
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)