TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCH30c02.yg.2.1
(722 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CW116836.1|CW116836 104_492_11107335_116_34608_054 Sorgh... 245 6e-064
gb|CW784439.1|CW784439 SP__Bb0020B10.f SP__Bb Sorghum propi... 177 4e-047
>gb|CW116836.1|CW116836 104_492_11107335_116_34608_054 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11107335, DNA
sequence
Length = 573
Score = 245 bits (529), Expect = 6e-064
Identities = 108/133 (81%), Positives = 108/133 (81%)
Frame = -2 / -1
Query: 670 ELXXXXXXXXIXXXXEQXAGXXEXSFSRWVXLXXXSKXXSMYIACSSYPFLDHDMFSVMA 491
EL I EQ AG E SFSRWV L SK SMYIAC SYPFLDHDMFSVMA
Sbjct: 399 ELYYSICRNEIRTIPEQGAGCSEMSFSRWVDLMWRSKRTSMYIACDSYPFLDHDMFSVMA 220
Query: 490 GPTVAAVSVVFDNVEHEEVLTGCIDGFLSVAKLAAFYHLGDVLNDLVVALSKFTTLLTTS 311
GPTVAAVSVVFDNVEHEEVLTGCIDGFLSVAKLAAFYHL DVLNDLVVALSKFTTLL TS
Sbjct: 219 GPTVAAVSVVFDNVEHEEVLTGCIDGFLSVAKLAAFYHLDDVLNDLVVALSKFTTLLNTS 40
Query: 310 YIDDPVTAFGEDT 272
YIDDPVTAFGEDT
Sbjct: 39 YIDDPVTAFGEDT 1
Score = 219 bits (473), Expect = 3e-056
Identities = 92/133 (69%), Positives = 102/133 (76%)
Frame = +2 / +1
Query: 272 GILPKRCYRIVNVGSGQQSGEFR*CHNKIIQNITKMIECSQFRH*QKAVNASCKNLFMLN 451
GILP+ CYRI+NV S QQSGEFR*CHNKII+NI KMIECSQF H Q+A+NASCKNLFMLN
Sbjct: 1 GILPESCYRIINVRSVQQSGEFR*CHNKIIENIIKMIECSQFCHRQEAINASCKNLFMLN 180
Query: 452 VIKNHRNSSNCWSGHNRKHVMIKKWVRATSNIH*XXL*PXXXXNPTTERXLXXPSXLLXX 631
++KNHRNSSNCWSGHN KHVMIK+ V TSN+H* L*P NPTTER L PS LL
Sbjct: 181 IVKNHRNSSNCWSGHNGKHVMIKERVGVTSNVH*CSL*PPH*INPTTERHLRTPSSLLRN 360
Query: 632 XXDXXXXXXXVKL 670
D +KL
Sbjct: 361 SSDFIPANRIIKL 399
Score = 192 bits (414), Expect = 5e-048
Identities = 91/123 (73%), Positives = 92/123 (74%)
Frame = +3 / +2
Query: 273 VSSPNAVTGSSM*EVVNKVVNLDXXXXXXXXXXXXXXNAASFATDRKPSMHPVRTSSCST 452
VSSP AVTGSSM*EV NKVVNLD NAASFATDRKPSMHPVRTSSCST
Sbjct: 2 VSSPKAVTGSSM*EVFNKVVNLDSATTRSLRTSSR**NAASFATDRKPSMHPVRTSSCST 181
Query: 453 LSKTTETAATVGPAITENMS*SRNG*ELQAIYIDXLFDXXXRXTQRLKXXSXXPAXCSXX 632
LSKTTETAATVGPAITENMS*SR G*E QA+YID LFD R TQRLK S PA CS
Sbjct: 182 LSKTTETAATVGPAITENMS*SRKG*ESQAMYIDVLFDLHIRSTQRLKDISEHPAPCSGI 361
Query: 633 XLI 641
LI
Sbjct: 362 VLI 370
Score = 131 bits (280), Expect(2) = 2e-039
Identities = 62/84 (73%), Positives = 64/84 (76%)
Frame = -1 / -3
Query: 641 NQXXX*AXSWVXXXXFQSLGXSXVXVKEXINVYCL*LLPIS*S*HVFCYGRTNSCCCFCG 462
NQ *A SWV FQSLG S V VKE INV+CL*LLP *S*HVF YGRTNSCCCFCG
Sbjct: 370 NQNYS*ARSWVF*DVFQSLG*SNVEVKENINVHCL*LLPFP*S*HVFRYGRTNSCCCFCG 191
Query: 461 F**R*A*RGSYRMH*RLSVSGETG 390
F* *A*RGSYRMH* L V G+TG
Sbjct: 190 F*QC*A*RGSYRMH*WLPVGGKTG 119
Score = 116 bits (249), Expect(2) = 7e-039
Identities = 48/58 (82%), Positives = 50/58 (86%)
Frame = +1 / +3
Query: 274 YPPQTLLQDRQCRKWSTKW*I*IVPQQDHSEHHQDDRMQPVSPLTESRQCIL*EPLHA 447
YPP+ LLQD QC+K STKW*I*IVPQQDH EHHQDDRMQPV P T S QCIL*EPLHA
Sbjct: 3 YPPRKLLQDHQCKKCSTKW*I*IVPQQDH*EHHQDDRMQPVLPPTGSHQCIL*EPLHA 176
Score = 81.3 bits (171), Expect(2) = 3e-025
Identities = 31/37 (83%), Positives = 34/37 (91%)
Frame = -3 / -2
Query: 450 LSMKRFLQDALTAFCQWRNWLHSIILVMF*MILLWHY 340
LSMKRFLQDAL A C+W+NWLHSIIL+MF MILLWHY
Sbjct: 179 LSMKRFLQDALMASCRWQNWLHSIILMMFSMILLWHY 69
Score = 65.2 bits (136), Expect(2) = 7e-039
Identities = 28/41 (68%), Positives = 30/41 (73%)
Frame = +1 / +3
Query: 502 KTCHDQEMGKSYKQYTLMXSLTXTXDXPND*KXSXXTQLXA 624
KTCHDQ G+S+KQ TLM SLT T D PND*K S TQL A
Sbjct: 231 KTCHDQGKGRSHKQCTLMFSLTSTLDQPND*KTSQNTQLLA 353
Score = 55.1 bits (114), Expect(2) = 3e-025
Identities = 26/40 (65%), Positives = 26/40 (65%)
Frame = -3 / -2
Query: 624 SXXLGXXRXLSVVGXIXCXGQRXHQCILLVALTHFLIMTC 505
S LG R LSVVG I C GQR HQC LLV T LIMTC
Sbjct: 353 SKELGVLRCLSVVGLI*CGGQREHQCTLLVTPTLSLIMTC 234
Score = 52.8 bits (109), Expect(2) = 2e-039
Identities = 21/25 (84%), Positives = 22/25 (88%)
Frame = -1 / -3
Query: 347 GTI*IHHFVDHFLH*RSCNSVWGGY 273
GTI*IHHFV+HFLH* SCNS GGY
Sbjct: 76 GTI*IHHFVEHFLH**SCNSFRGGY 2
>gb|CW784439.1|CW784439 SP__Bb0020B10.f SP__Bb Sorghum propinquum genomic clone
SP__Bb0020B10 5', DNA sequence
Length = 850
Score = 177 bits (382), Expect(2) = 4e-047
Identities = 71/113 (62%), Positives = 93/113 (82%)
Frame = -2 / -3
Query: 409 LSVAKLAAFYHLGDVLNDLVVALSKFTTLLTTSYIDDPVTAFGEDTKARMATEAVFTIAT 230
L V K++AF+HL DVL+DLVV+L KFTTLL TS +++PVTAFG+D KAR+ATE +FTIA
Sbjct: 848 LGVQKISAFHHLEDVLDDLVVSLCKFTTLLNTS*VEEPVTAFGDDLKARLATETLFTIAN 669
Query: 229 VYGDHIRSGWRNIIDCILRLHKIGLLPGPLTGDTADDQDSSSKSLPGKLASSA 71
YGD+IR+GWRN++DCILRLHK+GLLP + D ADD + S++++ GK A SA
Sbjct: 668 RYGDYIRTGWRNVLDCILRLHKLGLLPARVASDAADDSELSAEAVQGKAAPSA 510
Score = 31.8 bits (63), Expect(2) = 4e-047
Identities = 13/18 (72%), Positives = 15/18 (83%)
Frame = -2 / -3
Query: 55 ISTSRKTYGLMGRFSQLL 2
+ T RK+ GLMGRFSQLL
Sbjct: 485 MGTPRKSSGLMGRFSQLL 432
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 670,685,544
Number of Sequences: 832831
Number of extensions: 8314320
Number of successful extensions: 59475
Number of sequences better than 1.0e-020: 2
length of database: 163,786,556
effective HSP length: 54
effective length of database: 118,813,682
effective search space used: 22099344852
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)