TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCH2g03.yg.2.1
(537 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CD425534.1|CD425534 SA1_12_E03.g1_A002 Salicylic acid-tr... 106 3e-042
gb|BF656184.1|BF656184 FM1_44_A08.b1_A003 Floral-Induced Me... 172 4e-042
gb|CW474684.1|CW474684 fsbb001f230k23k0 Sorghum methylation... 132 3e-038
gb|BZ626646.1|BZ626646 ih47e08.b1 WGS-SbicolorF (DH5a methy... 71 1e-032
gb|CW408903.1|CW408903 fsbb001f102p21f0 Sorghum methylation... 76 4e-032
gb|BG054350.1|BG054350 OV2_3_C07.b1_A002 Ovary 2 (OV2) Sorg... 67 3e-024
gb|CW318957.1|CW318957 104_811_11474232_148_35883_090 Sorgh... 71 2e-023
gb|CW474683.1|CW474683 fsbb001f230k23f0 Sorghum methylation... 101 9e-021
>gb|CD425534.1|CD425534 SA1_12_E03.g1_A002 Salicylic acid-treated seedlings Sorghum bicolor
cDNA clone SA1_12_E03_A002 5', mRNA sequence
Length = 546
Score = 106 bits (227), Expect(2) = 3e-042
Identities = 45/62 (72%), Positives = 51/62 (82%)
Frame = +3 / +1
Query: 351 EPGQSLDQVIYKIKLPGNAILXEGKPENQXHAIIFTRXXXXQTIDMNQEHYMEEXLKMXN 530
+P Q+LDQVIY+IKLPG AIL EGKPENQ HAIIFTR QTID NQ++Y+EE LKM N
Sbjct: 277 DPIQNLDQVIYRIKLPGPAILGEGKPENQNHAIIFTRGEGLQTIDTNQDNYLEEALKMRN 456
Query: 531 LL 536
LL
Sbjct: 457 LL 462
Score = 86.3 bits (182), Expect(2) = 3e-042
Identities = 38/55 (69%), Positives = 42/55 (76%)
Frame = +3 / +1
Query: 105 LKTQCKAIADMKFTYVVSCQLYGIQKRSNEPCAHDILRLMTEYPSLRVAYIDEVE 269
L QC+A+ADMKFTYVVSCQ YG KR+ A DIL+LM Y SLRVAYIDEVE
Sbjct: 28 LFAQCEAVADMKFTYVVSCQQYGNDKRAALANAQDILQLMRNYSSLRVAYIDEVE 192
>gb|BF656184.1|BF656184 FM1_44_A08.b1_A003 Floral-Induced Meristem 1 (FM1) Sorghum
propinquum cDNA, mRNA sequence
Length = 413
Score = 172 bits (370), Expect = 4e-042
Identities = 74/82 (90%), Positives = 74/82 (90%)
Frame = +3 / +2
Query: 291 KKIEKVYYSVLVKASVTKPNEPGQSLDQVIYKIKLPGNAILXEGKPENQXHAIIFTRXXX 470
KKIEKVYYSVLVKASVTKPNEPGQSLDQVIYKIKLPGNAIL EGKPENQ HAIIFTR
Sbjct: 14 KKIEKVYYSVLVKASVTKPNEPGQSLDQVIYKIKLPGNAILGEGKPENQNHAIIFTRGEC 193
Query: 471 XQTIDMNQEHYMEEXLKMXNLL 536
QTIDMNQEHYMEE LKM NLL
Sbjct: 194 LQTIDMNQEHYMEEALKMRNLL 259
Score = 164 bits (352), Expect = 1e-039
Identities = 70/85 (82%), Positives = 72/85 (84%)
Frame = +2 / +1
Query: 281 R*NQED*KGLLLSVSEGFCYKT**AWSEP*SGHLQDKTTR*CHFX*RKARKPXPCNNIHP 460
R QED*KGLLL VS+GFCYKT**AWSEP*SGHLQDKTTR*CHF *RKARK PCNNIHP
Sbjct: 4 RGKQED*KGLLLRVSQGFCYKT**AWSEP*SGHLQDKTTR*CHFR*RKARKSEPCNNIHP 183
Query: 461 XRXXXNYRYESGALYGGXXENEXSA 535
R NYR+ESGALYGG ENE SA
Sbjct: 184 RRMSPNYRHESGALYGGGFENEKSA 258
Score = 131 bits (280), Expect = 1e-029
Identities = 55/67 (82%), Positives = 58/67 (86%)
Frame = -2 / -2
Query: 482 YSLXXXXSGEYYCMXLVFWLSFX*NGITW*FYLVDDLIKALTRLIRFCNRSLH*H*VVNL 303
YSL +GEYYCM L+FWLSF *NGITW*FYLVDDLIKALTRLIRFCNRSL *H VVNL
Sbjct: 205 YSLETFAAGEYYCMVLIFWLSFT*NGITW*FYLVDDLIKALTRLIRFCNRSLD*HGVVNL 26
Query: 302 FNLLDSI 282
FNLL S+
Sbjct: 25 FNLLVSL 5
Score = 123 bits (263), Expect = 2e-027
Identities = 57/66 (86%), Positives = 57/66 (86%)
Frame = -1 / -1
Query: 483 L*FXXXRXG*ILLHGSGFLAFLXLKWHYLVVLSCR*PDQGSDQAH*VL*QKPSLTLSSKP 304
L*F R G*ILLHGS FLAFL LKWHYLVVLSCR*PDQGSDQAH*VL*QKP LT SSKP
Sbjct: 206 L*FGDIRRG*ILLHGSDFLAFLHLKWHYLVVLSCR*PDQGSDQAH*VL*QKP*LTRSSKP 27
Query: 303 FQSS*F 286
FQSS F
Sbjct: 26 FQSSCF 9
Score = 119 bits (255), Expect = 3e-026
Identities = 58/84 (69%), Positives = 59/84 (70%)
Frame = +1 / +3
Query: 286 ESRRLKRFTTQCXXXXXXXXXXXXVRALIRSSTR*NYQVMPF*XKESQKTRXMQ*YSPXX 465
E+RRLKRFTT C VRALIRSSTR*NYQVMPF* KESQK R MQ*YSP
Sbjct: 9 ETRRLKRFTTPC*SRLLLQNLMSLVRALIRSSTR*NYQVMPF*VKESQKIRTMQ*YSPAA 188
Query: 466 XXXKL*I*IRSTIWRXX*K*XICC 537
KL* *IRSTIWR *K* ICC
Sbjct: 189 NVSKL*T*IRSTIWRRL*K*EICC 260
>gb|CW474684.1|CW474684 fsbb001f230k23k0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f230k23, DNA
sequence
Length = 678
Score = 132 bits (282), Expect(2) = 3e-038
Identities = 56/66 (84%), Positives = 58/66 (87%)
Frame = +3 / +1
Query: 54 DLMKGYRAMEVMSEDSPLKTQCKAIADMKFTYVVSCQLYGIQKRSNEPCAHDILRLMTEY 233
DLM+GYRA EVM EDS L TQCKAIADMKFTYVVSCQ YGIQKRSNEPCAHDILRLMTE
Sbjct: 253 DLMEGYRATEVMPEDSQLMTQCKAIADMKFTYVVSCQQYGIQKRSNEPCAHDILRLMTE* 432
Query: 234 PSLRVA 251
SLR +
Sbjct: 433 VSLRTS 450
Score = 121 bits (259), Expect(2) = 6e-036
Identities = 54/65 (83%), Positives = 58/65 (89%)
Frame = -3 / -2
Query: 244 LSDGYSVISLKISCAQGSLERF*IPYSWHETTYVNFMSAIALH*VFNGESSDITSIAL*P 65
L + +SVISLKISCAQGSLERF*IPY WHETTYVNFMSAIALH*V N ESS ITS+AL*P
Sbjct: 443 LKETHSVISLKISCAQGSLERF*IPYCWHETTYVNFMSAIALH*VINCESSGITSVAL*P 264
Query: 64 FIRSS 50
IRS+
Sbjct: 263 SIRSN 249
Score = 119 bits (255), Expect(2) = 1e-031
Identities = 53/60 (88%), Positives = 53/60 (88%)
Frame = +2 / +3
Query: 56 SYERLQSNGSNV*GLTIKDSMQGYSRHEVYICCLMPTIWNSEAF**ALRT*YFETNDRVS 235
SY RLQSN SN *GLTI DSMQGYS HEVYICCLMPTIWNSEAF**AL T*YFETND VS
Sbjct: 255 SYGRLQSNRSNA*GLTINDSMQGYS*HEVYICCLMPTIWNSEAF**ALCT*YFETNDGVS 434
Score = 117 bits (250), Expect(2) = 3e-031
Identities = 52/59 (88%), Positives = 54/59 (91%)
Frame = -1 / -3
Query: 231 TLSLVSKYHVRKAH*NASEFHIVGMRQHM*TSCRL*PCIESLMVSPQTLLPLLCSLS*D 55
T SLVSKYHV KAH*NASEFHIVGMRQHM*TSC+L*PCIESL+VSPQ LL LLCSL *D
Sbjct: 430 TPSLVSKYHVHKAH*NASEFHIVGMRQHM*TSCQL*PCIESLIVSPQALLLLLCSLP*D 254
Score = 111 bits (238), Expect(2) = 2e-027
Identities = 52/59 (88%), Positives = 52/59 (88%)
Frame = -2 / -1
Query: 230 LCH*SQNIMCARLIRTLLNSI*LA*DNICKLHVGYSLALSL*W*VLRHYFHCSVAFHKI 54
L H*SQNIMC RLIRTLLNSI LA*DNICKLHV YSLALS * *VLRHYF CSVAFHKI
Sbjct: 429 LRH*SQNIMCTRLIRTLLNSILLA*DNICKLHVSYSLALSH*L*VLRHYFCCSVAFHKI 253
Score = 101 bits (216), Expect(2) = 4e-026
Identities = 51/59 (86%), Positives = 53/59 (89%)
Frame = +1 / +2
Query: 55 IL*KATEQWK*CLRTHH*RLNARL*PT*SLHMLSHANYMEFRSVLMSLAHMIF*D**QS 231
IL KATEQ K*CLRTH+* LNARL* T*SLHMLSHAN MEFRSVLMSL HMIF*D**+S
Sbjct: 254 ILWKATEQQK*CLRTHN**LNARL*LT*SLHMLSHANNMEFRSVLMSLVHMIF*D**RS 430
Score = 50.1 bits (103), Expect(2) = 6e-036
Identities = 17/18 (94%), Positives = 17/18 (94%)
Frame = -1 / -2
Query: 54 HHCWPYQEKPEAPKLFDS 1
HH WPYQEKPEAPKLFDS
Sbjct: 158 HHLWPYQEKPEAPKLFDS 105
Score = 47.3 bits (97), Expect(2) = 3e-038
Identities = 17/19 (89%), Positives = 18/19 (94%)
Frame = +1 / +3
Query: 1 TIEKLWSFRLFLIWPTMMI 57
TIEKLWSFRLFLIWP MM+
Sbjct: 105 TIEKLWSFRLFLIWPKMMV 161
Score = 38.6 bits (78), Expect(2) = 3e-031
Identities = 17/22 (77%), Positives = 17/22 (77%)
Frame = -2 / -3
Query: 68 AFHKIIIVGHIKKSLKLQSFSI 3
A II GHIKKSLKLQSFSI
Sbjct: 172 AIRNTIIFGHIKKSLKLQSFSI 107
Score = 37.7 bits (76), Expect(2) = 1e-031
Identities = 15/19 (78%), Positives = 17/19 (89%)
Frame = +3 / +2
Query: 3 YRKALELQAFLDMANNDDL 59
YRKALELQAFLDMA +D +
Sbjct: 107 YRKALELQAFLDMAKDDGI 163
Score = 36.8 bits (74), Expect(2) = 4e-026
Identities = 16/17 (94%), Positives = 17/17 (100%)
Frame = +2 / +1
Query: 2 LSKSFGASGFS*YGQQ* 52
LSKSFGASGFS*YGQ+*
Sbjct: 106 LSKSFGASGFS*YGQR* 156
Score = 31.3 bits (62), Expect(2) = 2e-027
Identities = 16/17 (94%), Positives = 16/17 (94%)
Frame = -3 / -1
Query: 52 SLLAISRKA*SSKAFR* 2
S LAISRKA*SSKAFR*
Sbjct: 156 SSLAISRKA*SSKAFR* 106
>gb|BZ626646.1|BZ626646 ih47e08.b1 WGS-SbicolorF (DH5a methyl filtered) Sorghum bicolor
genomic clone ih47e08 5', DNA sequence
Length = 714
Score = 70.7 bits (148), Expect(4) = 1e-032
Identities = 30/39 (76%), Positives = 31/39 (79%)
Frame = +3 / -1
Query: 378 IYKIKLPGNAILXEGKPENQXHAIIFTRXXXXQTIDMNQ 494
IY+IKLPG A L EGKPENQ HAIIFTR QTIDMNQ
Sbjct: 123 IYRIKLPGPAKLGEGKPENQNHAIIFTRGEALQTIDMNQ 7
Score = 60.6 bits (126), Expect(4) = 1e-032
Identities = 26/52 (50%), Positives = 34/52 (65%)
Frame = +3 / -2
Query: 75 AMEVMSEDSPLKTQCKAIADMKFTYVVSCQLYGIQKRSNEPCAHDILRLMTE 230
A E L +Q +A+ADMKFTYV +CQ+YG QK+S + A DIL LM +
Sbjct: 677 AEEEKKSQRSLSSQLEAVADMKFTYVATCQIYGNQKQSGDRRATDILNLMVK 522
Score = 38.2 bits (77), Expect(4) = 1e-032
Identities = 14/23 (60%), Positives = 18/23 (78%)
Frame = +3 / -2
Query: 231 YPSLRVAYIDEVEAPSKDRIKKI 299
YP LRVAYIDEVE D+++K+
Sbjct: 326 YPGLRVAYIDEVEEREGDKVQKV 258
Score = 27.6 bits (54), Expect(4) = 1e-032
Identities = 10/13 (76%), Positives = 13/13 (100%)
Frame = +3 / -2
Query: 294 KIEKVYYSVLVKA 332
K++KV+YSVLVKA
Sbjct: 272 KVQKVFYSVLVKA 234
>gb|CW408903.1|CW408903 fsbb001f102p21f0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f102p21, DNA
sequence
Length = 704
Score = 76.2 bits (160), Expect(4) = 4e-032
Identities = 32/43 (74%), Positives = 35/43 (81%)
Frame = +3 / +2
Query: 366 LDQVIYKIKLPGNAILXEGKPENQXHAIIFTRXXXXQTIDMNQ 494
+ +VIY+IKLPG AIL EGKPENQ HAIIFTR QTIDMNQ
Sbjct: 545 VSKVIYRIKLPGPAILGEGKPENQNHAIIFTRGEGLQTIDMNQ 673
Score = 57.4 bits (119), Expect(4) = 4e-032
Identities = 24/36 (66%), Positives = 28/36 (77%)
Frame = +3 / +2
Query: 117 CKAIADMKFTYVVSCQLYGIQKRSNEPCAHDILRLM 224
C+A+ADMKFTYVVSCQ YG KR+ A DIL+LM
Sbjct: 2 CEAVADMKFTYVVSCQQYGNDKRAALANAQDILQLM 109
Score = 32.2 bits (64), Expect(4) = 4e-032
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = +3 / +3
Query: 291 KKIEKVYYSVLVKASVTKPNEPGQSLDQV 377
KK+E YYS LVK ++TK +E + +
Sbjct: 312 KKMETAYYSTLVKVALTKDSESADPIQNL 398
Score = 29.5 bits (58), Expect(4) = 4e-032
Identities = 12/13 (92%), Positives = 12/13 (92%)
Frame = +3 / +3
Query: 231 YPSLRVAYIDEVE 269
Y SLRVAYIDEVE
Sbjct: 258 YSSLRVAYIDEVE 296
>gb|BG054350.1|BG054350 OV2_3_C07.b1_A002 Ovary 2 (OV2) Sorghum bicolor cDNA, mRNA sequence
Length = 466
Score = 66.6 bits (139), Expect(3) = 3e-024
Identities = 29/39 (74%), Positives = 29/39 (74%)
Frame = +3 / +2
Query: 369 DQVIYKIKLPGNAILXEGKPENQXHAIIFTRXXXXQTID 485
DQ IY IKLPGN L EGKPENQ HAIIFTR QTID
Sbjct: 350 DQEIYCIKLPGNPKLGEGKPENQNHAIIFTRGDAVQTID 466
Score = 61.6 bits (128), Expect(3) = 3e-024
Identities = 29/60 (48%), Positives = 38/60 (63%)
Frame = +3 / +2
Query: 105 LKTQCKAIADMKFTYVVSCQLYGIQKRSNEPCAHDILRLMTEYPSLRVAYIDEVEAPSKD 284
L +A AD+K TYVVSCQ+YG QK+ + A DI L+ +LRVA+I E E S+D
Sbjct: 110 LSPDARAQADIKLTYVVSCQIYGQQKQMKKQEAADIALLLQRNEALRVAFIHEEENISRD 289
Score = 22.1 bits (42), Expect(3) = 3e-024
Identities = 10/16 (62%), Positives = 10/16 (62%)
Frame = +3 / +2
Query: 291 KKIEKVYYSVLVKASV 338
K K YYS LVKA V
Sbjct: 293 KATTKEYYSKLVKADV 340
>gb|CW318957.1|CW318957 104_811_11474232_148_35883_090 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11474232, DNA
sequence
Length = 750
Score = 70.7 bits (148), Expect(4) = 2e-023
Identities = 30/39 (76%), Positives = 31/39 (79%)
Frame = +3 / +3
Query: 378 IYKIKLPGNAILXEGKPENQXHAIIFTRXXXXQTIDMNQ 494
IY+IKLPG A L EGKPENQ HAIIFTR QTIDMNQ
Sbjct: 312 IYRIKLPGPAKLGEGKPENQNHAIIFTRGEALQTIDMNQ 428
Score = 38.2 bits (77), Expect(4) = 2e-023
Identities = 14/23 (60%), Positives = 18/23 (78%)
Frame = +3 / +1
Query: 231 YPSLRVAYIDEVEAPSKDRIKKI 299
YP LRVAYIDEVE D+++K+
Sbjct: 109 YPGLRVAYIDEVEEREGDKVQKV 177
Score = 27.6 bits (54), Expect(4) = 2e-023
Identities = 10/15 (66%), Positives = 13/15 (86%)
Frame = +3 / +3
Query: 492 QEHYMEEXLKMXNLL 536
Q++Y+EE LKM NLL
Sbjct: 498 QDNYLEEALKMRNLL 542
Score = 27.6 bits (54), Expect(4) = 2e-023
Identities = 10/13 (76%), Positives = 13/13 (100%)
Frame = +3 / +1
Query: 294 KIEKVYYSVLVKA 332
K++KV+YSVLVKA
Sbjct: 163 KVQKVFYSVLVKA 201
>gb|CW474683.1|CW474683 fsbb001f230k23f0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f230k23, DNA
sequence
Length = 613
Score = 101 bits (215), Expect = 9e-021
Identities = 42/47 (89%), Positives = 43/47 (91%)
Frame = +3 / -2
Query: 111 TQCKAIADMKFTYVVSCQLYGIQKRSNEPCAHDILRLMTEYPSLRVA 251
TQCKAIADMKFTYVVSCQ YGIQKRSNEPCAHDILRLMTE SLR +
Sbjct: 609 TQCKAIADMKFTYVVSCQQYGIQKRSNEPCAHDILRLMTE*VSLRTS 469
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 502,968,696
Number of Sequences: 832831
Number of extensions: 5720660
Number of successful extensions: 40020
Number of sequences better than 1.0e-020: 8
length of database: 163,786,556
effective HSP length: 53
effective length of database: 119,646,513
effective search space used: 14955814125
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)