TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBTB.006E19F020311.3.1
(789 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CW130838.1|CW130838 104_513_11115338_148_34779_007 Sorgh... 104 4e-034
gb|CL185860.1|CL185860 104_400_10899829_116_32404_059 Sorgh... 101 1e-027
gb|CW130774.1|CW130774 104_513_11115303_148_34778_058 Sorgh... 58 3e-026
gb|CW153395.1|CW153395 104_555_11145024_116_36346_096 Sorgh... 94 2e-025
gb|CW331360.1|CW331360 104_828_11480862_116_36026_021 Sorgh... 94 3e-021
>gb|CW130838.1|CW130838 104_513_11115338_148_34779_007 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11115338, DNA
sequence
Length = 659
Score = 104 bits (222), Expect(2) = 4e-034
Identities = 41/55 (74%), Positives = 46/55 (83%)
Frame = +2 / +3
Query: 479 VMLTYLVYEKQQKLRIMMKMHGLKDGPYWLISYSYFLALSIVYMLFFMIFGSLIG 643
VMLTYLVYEKQ KLR MMKMHGL DGPYW+I Y YFL S +YM+ F+IFGS+IG
Sbjct: 417 VMLTYLVYEKQHKLRTMMKMHGLGDGPYWIIYYMYFLIFSTLYMIVFVIFGSVIG 581
Score = 63.8 bits (133), Expect(2) = 4e-034
Identities = 26/54 (48%), Positives = 34/54 (62%)
Frame = +2 / +1
Query: 320 VSNAYLKLIKGTGVDMLLEFVKDMPKVGTXXXXXXXXXXXXXXXTWIIELLFPV 481
VSNAYL+LI+G+GV+MLL+F K+MPK T W++ LLFPV
Sbjct: 127 VSNAYLQLIRGSGVNMLLDFTKEMPKQATRLTFDFSAVAGPLFFEWVVVLLFPV 288
>gb|CL185860.1|CL185860 104_400_10899829_116_32404_059 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10899829, DNA
sequence
Length = 719
Score = 101 bits (215), Expect(2) = 1e-027
Identities = 39/59 (66%), Positives = 47/59 (79%)
Frame = +2 / +3
Query: 473 FPVMLTYLVYEKQQKLRIMMKMHGLKDGPYWLISYSYFLALSIVYMLFFMIFGSLIGLN 649
F V +T LVYEK+ KLR+MMKMHGLK+ PYWLI+Y+YFL LS Y+ MIFGSL+G N
Sbjct: 3 FQVTVTLLVYEKEHKLRLMMKMHGLKNAPYWLITYAYFLCLSTAYITLLMIFGSLLGTN 179
Score = 43.2 bits (88), Expect(2) = 1e-027
Identities = 17/43 (39%), Positives = 23/43 (53%)
Frame = +2 / +3
Query: 641 GLNFFRVNEYSIQAVFFFVCINLQIXXXXXXXXXXXXGKMATG 769
GL+ FR+N Y +Q +F+F INLQI K A+G
Sbjct: 267 GLDIFRLNNYGVQFIFYFTYINLQIALAFLFASFFSSTKTASG 395
>gb|CW130774.1|CW130774 104_513_11115303_148_34778_058 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11115303, DNA
sequence
Length = 653
Score = 58.3 bits (121), Expect(3) = 3e-026
Identities = 23/31 (74%), Positives = 26/31 (83%)
Frame = +2 / +2
Query: 461 IELLFPVMLTYLVYEKQQKLRIMMKMHGLKD 553
I V+LTYLVYEKQQ+LR+MMKMHGLKD
Sbjct: 560 ISFFLQVILTYLVYEKQQRLRLMMKMHGLKD 652
Score = 51.5 bits (106), Expect(3) = 3e-026
Identities = 20/43 (46%), Positives = 29/43 (67%)
Frame = +2 / +2
Query: 194 GYDFLGTTQYGLGVNVWYNSTYNDNNAYSFISTLRVPRLVNAV 322
GYDFL T + + VWYNS+++ +N + ++ LRV RLVN V
Sbjct: 74 GYDFLDTNKRRFHIYVWYNSSFSRDNGHHSMTVLRVSRLVNMV 202
Score = 50.6 bits (104), Expect(3) = 3e-026
Identities = 20/53 (37%), Positives = 26/53 (49%)
Frame = +2 / +3
Query: 323 SNAYLKLIKGTGVDMLLEFVKDMPKVGTXXXXXXXXXXXXXXXTWIIELLFPV 481
S AYL +KG +M LEF+K+MPK TW ++LL PV
Sbjct: 348 STAYLNFLKGENAEMRLEFLKEMPKAAVKTRLDFTTLLDALFFTWTVQLLLPV 506
>gb|CW153395.1|CW153395 104_555_11145024_116_36346_096 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11145024, DNA
sequence
Length = 693
Score = 93.6 bits (198), Expect(2) = 2e-025
Identities = 35/51 (68%), Positives = 42/51 (82%)
Frame = +2 / +1
Query: 497 VYEKQQKLRIMMKMHGLKDGPYWLISYSYFLALSIVYMLFFMIFGSLIGLN 649
VYEK+ KLR+MMKMHGLK+ PYWLI+Y+YFL LS Y+ MIFGSL+G N
Sbjct: 1 VYEKEHKLRLMMKMHGLKNAPYWLITYAYFLCLSTAYITLLMIFGSLLGTN 153
Score = 43.2 bits (88), Expect(2) = 2e-025
Identities = 17/43 (39%), Positives = 23/43 (53%)
Frame = +2 / +1
Query: 641 GLNFFRVNEYSIQAVFFFVCINLQIXXXXXXXXXXXXGKMATG 769
GL+ FR+N Y +Q +F+F INLQI K A+G
Sbjct: 241 GLDIFRLNNYGVQFIFYFTYINLQIALAFLFASFFSSTKTASG 369
>gb|CW331360.1|CW331360 104_828_11480862_116_36026_021 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11480862, DNA
sequence
Length = 671
Score = 94.1 bits (199), Expect(2) = 3e-021
Identities = 38/59 (64%), Positives = 46/59 (77%)
Frame = +2 / +3
Query: 467 LLFPVMLTYLVYEKQQKLRIMMKMHGLKDGPYWLISYSYFLALSIVYMLFFMIFGSLIG 643
LL V+L+ LVYEK+ KLR +MKMHGL D YW+ISY YFL LS++YMLF + FGSL G
Sbjct: 273 LLLQVILSNLVYEKEHKLRTIMKMHGLGDMAYWIISYCYFLLLSLLYMLFLVFFGSLFG 449
Score = 30.9 bits (61), Expect(2) = 3e-021
Identities = 10/19 (52%), Positives = 14/19 (73%)
Frame = +2 / +3
Query: 344 IKGTGVDMLLEFVKDMPKV 400
++G G M EF+KDMP+V
Sbjct: 27 LRGNGTKMQFEFIKDMPRV 83
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 990,651,054
Number of Sequences: 832831
Number of extensions: 13903026
Number of successful extensions: 77290
Number of sequences better than 1.0e-020: 5
length of database: 163,786,556
effective HSP length: 54
effective length of database: 118,813,682
effective search space used: 24713245856
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)