TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBJ4h04.xg.2.2
(494 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CW071272.1|CW071272 104_323_10591451_114_30471 Sorghum m... 90 4e-035
gb|CW376890.1|CW376890 fsbb001f054p19f0 Sorghum methylation... 90 9e-033
gb|CN127879.1|CN127879 RHOH1_25_D05.g1_A002 Acid- and alkal... 94 2e-031
gb|AW671472.1|AW671472 LG1_347_C12.b1_A002 Light Grown 1 (L... 105 2e-031
gb|BE360223.1|BE360223 DG1_62_C07.b1_A002 Dark Grown 1 (DG1... 94 3e-031
gb|CN140719.1|CN140719 OX1_46_B10.g1_A002 Oxidatively-stres... 91 1e-030
gb|CN129542.1|CN129542 RHOH1_36_B03.b3_A002 Acid- and alkal... 86 3e-029
gb|CN129628.1|CN129628 RHOH1_36_B03.g1_A002 Acid- and alkal... 86 3e-029
gb|CW376891.1|CW376891 fsbb001f054p19k0 Sorghum methylation... 90 7e-027
gb|AW287022.2|AW287022 LG1_264_E07.b1_A002 Light Grown 1 (L... 94 3e-026
gb|CW497105.1|CW497105 fsbb001f289k18f0 Sorghum methylation... 80 2e-025
gb|CN127798.1|CN127798 RHOH1_25_D05.b1_A002 Acid- and alkal... 70 2e-024
>gb|CW071272.1|CW071272 104_323_10591451_114_30471 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 10591451, DNA
sequence
Length = 734
Score = 90.0 bits (190), Expect(2) = 4e-035
Identities = 34/48 (70%), Positives = 35/48 (72%)
Frame = +2 / -1
Query: 65 GTMSFTGXQDKCTXCDKTXHFIDLLTXXXXIYHKTCXXXXHCKGXXSM 208
GTMSFTG QDKCT CDKT HFIDLLT IYHKTC HCKG S+
Sbjct: 521 GTMSFTGTQDKCTACDKTVHFIDLLTADGVIYHKTCFKCSHCKGILSV 378
Score = 80.3 bits (169), Expect(2) = 4e-035
Identities = 31/39 (79%), Positives = 31/39 (79%)
Frame = +2 / -1
Query: 188 CKGXXSMCSYSSMDXXLYCKTHFEQLFKXTGSFSKNFTP 304
C G MCSYSSMD LYCKTHFEQLFK TGSFSK FTP
Sbjct: 239 CVGRLQMCSYSSMDGVLYCKTHFEQLFKETGSFSKKFTP 123
Score = 72.1 bits (151), Expect(2) = 2e-022
Identities = 27/40 (67%), Positives = 27/40 (67%)
Frame = +1 / -3
Query: 67 HHVFHRXAGQVHXXRQDXPLHRPPHXXXXHLP*DMXQXXP 186
HHVFHR AGQVH RQD P HRPPH HLP DM Q P
Sbjct: 519 HHVFHRHAGQVHGVRQDRPFHRPPHGRRRHLPQDMLQVQP 400
Score = 70.7 bits (148), Expect(2) = 6e-025
Identities = 30/43 (69%), Positives = 31/43 (72%)
Frame = -3 / +1
Query: 303 GVKFLEKLPVXLKSCSKWVLQYXXXSMEE*LHIEXXPLQWXXL 175
GV FLEKLPV LKSCSKWVLQY SMEE*LHI P + L
Sbjct: 124 GVNFLEKLPVSLKSCSKWVLQYSTPSMEE*LHICSRPTHYTSL 252
Score = 65.2 bits (136), Expect(2) = 6e-025
Identities = 28/42 (66%), Positives = 29/42 (69%)
Frame = -1 / +2
Query: 191 CSGXX*XMSYGRWXXXX*GGR*SGXSCRXXCTCPAXR*KTWC 66
CSG * MS GR *GGR*+G SCR CTCPA R*KTWC
Sbjct: 395 CSGCT*SMSCGR*RRRP*GGR*NGRSCRTPCTCPACR*KTWC 520
Score = 55.6 bits (115), Expect(2) = 2e-022
Identities = 23/27 (85%), Positives = 24/27 (88%)
Frame = +1 / -3
Query: 223 HGRXXVLQDPLRAALQXDRELLQELHA 303
HGR VLQDPLRAALQ DRELLQE+HA
Sbjct: 204 HGRCAVLQDPLRAALQGDRELLQEVHA 124
>gb|CW376890.1|CW376890 fsbb001f054p19f0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f054p19, DNA
sequence
Length = 642
Score = 90.0 bits (190), Expect(2) = 9e-033
Identities = 34/48 (70%), Positives = 35/48 (72%)
Frame = +2 / +3
Query: 65 GTMSFTGXQDKCTXCDKTXHFIDLLTXXXXIYHKTCXXXXHCKGXXSM 208
GTMSFTG QDKCT CDKT HFIDLLT IYHKTC HCKG S+
Sbjct: 252 GTMSFTGTQDKCTACDKTVHFIDLLTADGVIYHKTCFKCSHCKGILSV 395
Score = 72.1 bits (151), Expect(2) = 9e-033
Identities = 28/35 (80%), Positives = 28/35 (80%)
Frame = +2 / +3
Query: 188 CKGXXSMCSYSSMDXXLYCKTHFEQLFKXTGSFSK 292
C G MCSYSSMD LYCKTHFEQLFK TGSFSK
Sbjct: 534 CVGRLQMCSYSSMDGVLYCKTHFEQLFKETGSFSK 638
Score = 72.1 bits (151), Expect(2) = 9e-021
Identities = 27/40 (67%), Positives = 27/40 (67%)
Frame = +1 / +2
Query: 67 HHVFHRXAGQVHXXRQDXPLHRPPHXXXXHLP*DMXQXXP 186
HHVFHR AGQVH RQD P HRPPH HLP DM Q P
Sbjct: 254 HHVFHRHAGQVHGVRQDRPFHRPPHGRRRHLPQDMLQVQP 373
Score = 66.1 bits (138), Expect(2) = 1e-023
Identities = 28/40 (70%), Positives = 29/40 (72%)
Frame = -3 / -3
Query: 294 FLEKLPVXLKSCSKWVLQYXXXSMEE*LHIEXXPLQWXXL 175
FLEKLPV LKSCSKWVLQY SMEE*LHI P + L
Sbjct: 640 FLEKLPVSLKSCSKWVLQYSTPSMEE*LHICSRPTHYTSL 521
Score = 65.2 bits (136), Expect(2) = 1e-023
Identities = 28/42 (66%), Positives = 29/42 (69%)
Frame = -1 / -1
Query: 191 CSGXX*XMSYGRWXXXX*GGR*SGXSCRXXCTCPAXR*KTWC 66
CSG * MS GR *GGR*+G SCR CTCPA R*KTWC
Sbjct: 378 CSGCT*SMSCGR*RRRP*GGR*NGRSCRTPCTCPACR*KTWC 253
Score = 49.6 bits (102), Expect(2) = 9e-021
Identities = 21/24 (87%), Positives = 21/24 (87%)
Frame = +1 / +2
Query: 223 HGRXXVLQDPLRAALQXDRELLQE 294
HGR VLQDPLRAALQ DRELLQE
Sbjct: 569 HGRCAVLQDPLRAALQGDRELLQE 640
>gb|CN127879.1|CN127879 RHOH1_25_D05.g1_A002 Acid- and alkaline-treated roots Sorghum
bicolor cDNA clone RHOH1_25_D05_A002 5', mRNA sequence
Length = 692
Score = 93.6 bits (198), Expect(2) = 2e-031
Identities = 34/74 (45%), Positives = 47/74 (63%)
Frame = +2 / +3
Query: 77 FTGXQDKCTXCDKTXHFIDLLTXXXXIYHKTCXXXXHCKGXXSMCSYSSMDXXLYCKTHF 256
F+G Q KC C KT + +D L+ +H++C HCK S+ +YSS + YCK HF
Sbjct: 69 FSGTQQKCKVCTKTVYPMDQLSTDGVAFHRSCFKCQHCKSTLSLSNYSSFEGVPYCKAHF 248
Query: 257 EQLFKXTGSFSKNF 298
EQLFK TGS++K+F
Sbjct: 249 EQLFKETGSYNKSF 290
Score = 62.9 bits (131), Expect(2) = 2e-031
Identities = 26/54 (48%), Positives = 31/54 (57%)
Frame = +2 / +3
Query: 332 LTRAPSKLSSAFSGTXDKXAACQKTXYPXXXLTLXXXXYHKXXXKCSXXXXXLT 493
LTR+PSK + FSGT DK A C KT YP +T+ YHK KCS +T
Sbjct: 339 LTRSPSKAARMFSGTQDKCATCGKTAYPLEKVTVEEKAYHKSCFKCSHGGCAIT 500
>gb|AW671472.1|AW671472 LG1_347_C12.b1_A002 Light Grown 1 (LG1) Sorghum bicolor cDNA, mRNA
sequence
Length = 674
Score = 105 bits (224), Expect(2) = 2e-031
Identities = 38/75 (50%), Positives = 47/75 (62%)
Frame = +2 / +1
Query: 74 SFTGXQDKCTXCDKTXHFIDLLTXXXXIYHKTCXXXXHCKGXXSMCSYSSMDXXLYCKTH 253
SF G KCT CDKT + +D LT IYHK C HCKG + +Y+S + LYC+ H
Sbjct: 229 SFQGTTTKCTACDKTVYLVDKLTADNRIYHKACFRCHHCKGTLKLANYNSFEGVLYCRPH 408
Query: 254 FEQLFKXTGSFSKNF 298
F+QLFK TGS K+F
Sbjct: 409 FDQLFKRTGSLDKSF 453
Score = 51.0 bits (105), Expect(2) = 2e-031
Identities = 21/57 (36%), Positives = 28/57 (49%)
Frame = +2 / +1
Query: 299 TPXXXSSDKXXLTRAPSKLSSAFSGTXDKXAACQKTXYPXXXLTLXXXXYHKXXXKC 469
TP ++ K+SSAF+GT +K C KT YP +T+ YHK KC
Sbjct: 460 TPKVVKPERNVGNENAVKVSSAFAGTREKCVGCSKTVYPIEKVTVNNTMYHKSCFKC 630
>gb|BE360223.1|BE360223 DG1_62_C07.b1_A002 Dark Grown 1 (DG1) Sorghum bicolor cDNA, mRNA
sequence
Length = 597
Score = 93.6 bits (198), Expect(2) = 3e-031
Identities = 34/74 (45%), Positives = 47/74 (63%)
Frame = +2 / +2
Query: 77 FTGXQDKCTXCDKTXHFIDLLTXXXXIYHKTCXXXXHCKGXXSMCSYSSMDXXLYCKTHF 256
F+G Q KC C KT + +D L+ +H++C HCK S+ +YSS + YCK HF
Sbjct: 149 FSGTQQKCKVCTKTVYPMDQLSTDGVAFHRSCFKCQHCKSTLSLSNYSSFEGVPYCKAHF 328
Query: 257 EQLFKXTGSFSKNF 298
EQLFK TGS++K+F
Sbjct: 329 EQLFKETGSYNKSF 370
Score = 62.0 bits (129), Expect(2) = 3e-031
Identities = 25/47 (53%), Positives = 29/47 (61%)
Frame = +2 / +2
Query: 332 LTRAPSKLSSAFSGTXDKXAACQKTXYPXXXLTLXXXXYHKXXXKCS 472
LTR+PSK + FSGT DK A C KT YP +T+ YHK KCS
Sbjct: 419 LTRSPSKAARMFSGTQDKCATCGKTAYPLEKVTVEEKAYHKSCFKCS 559
>gb|CN140719.1|CN140719 OX1_46_B10.g1_A002 Oxidatively-stressed leaves and roots Sorghum
bicolor cDNA clone OX1_46_B10_A002 5', mRNA sequence
Length = 544
Score = 90.9 bits (192), Expect(2) = 1e-030
Identities = 34/78 (43%), Positives = 47/78 (60%)
Frame = +2 / +1
Query: 65 GTMSFTGXQDKCTXCDKTXHFIDLLTXXXXIYHKTCXXXXHCKGXXSMCSYSSMDXXLYC 244
G F+G Q KC C KT + +D L+ +H++C HCK S+ +YSS + YC
Sbjct: 46 GEKMFSGTQQKCKVCTKTVYPMDQLSTDGVAFHRSCFKCQHCKSTLSLSNYSSFEGVPYC 225
Query: 245 KTHFEQLFKXTGSFSKNF 298
K HFEQLFK T S++K+F
Sbjct: 226 KAHFEQLFKETVSYNKSF 279
Score = 62.9 bits (131), Expect(2) = 1e-030
Identities = 26/54 (48%), Positives = 31/54 (57%)
Frame = +2 / +1
Query: 332 LTRAPSKLSSAFSGTXDKXAACQKTXYPXXXLTLXXXXYHKXXXKCSXXXXXLT 493
LTR+PSK + FSGT DK A C KT YP +T+ YHK KCS +T
Sbjct: 328 LTRSPSKAARMFSGTQDKCATCGKTAYPLEKVTVEEKAYHKSCFKCSHGGCAIT 489
>gb|CN129542.1|CN129542 RHOH1_36_B03.b3_A002 Acid- and alkaline-treated roots Sorghum
bicolor cDNA clone RHOH1_36_B03_A002 3', mRNA sequence
Length = 819
Score = 86.3 bits (182), Expect(2) = 3e-029
Identities = 34/74 (45%), Positives = 46/74 (62%)
Frame = +2 / +2
Query: 77 FTGXQDKCTXCDKTXHFIDLLTXXXXIYHKTCXXXXHCKGXXSMCSYSSMDXXLYCKTHF 256
F+G Q KC KT + +D L+ H++C HCK S+ +YSS + YCK HF
Sbjct: 11 FSGTQQKCKVRTKTVYPMDQLSTXGVALHRSCFKCQHCKSTLSLSNYSSFEGVPYCKAHF 190
Query: 257 EQLFKXTGSFSKNF 298
EQLFK TGS++K+F
Sbjct: 191 EQLFKETGSYNKSF 232
Score = 62.9 bits (131), Expect(2) = 3e-029
Identities = 26/54 (48%), Positives = 31/54 (57%)
Frame = +2 / +2
Query: 332 LTRAPSKLSSAFSGTXDKXAACQKTXYPXXXLTLXXXXYHKXXXKCSXXXXXLT 493
LTR+PSK + FSGT DK A C KT YP +T+ YHK KCS +T
Sbjct: 281 LTRSPSKAARMFSGTQDKCATCGKTAYPLEKVTVEEKAYHKSCFKCSHGGCAIT 442
>gb|CN129628.1|CN129628 RHOH1_36_B03.g1_A002 Acid- and alkaline-treated roots Sorghum
bicolor cDNA clone RHOH1_36_B03_A002 5', mRNA sequence
Length = 784
Score = 86.3 bits (182), Expect(2) = 3e-029
Identities = 33/74 (44%), Positives = 45/74 (60%)
Frame = +2 / +2
Query: 77 FTGXQDKCTXCDKTXHFIDLLTXXXXIYHKTCXXXXHCKGXXSMCSYSSMDXXLYCKTHF 256
F+G Q KC KT + +D L+ H++C HCK S+ +YSS + YCK HF
Sbjct: 113 FSGTQQKCKVRTKTVYPMDQLSTDGVALHRSCFKCQHCKSTLSLSNYSSFEGVPYCKAHF 292
Query: 257 EQLFKXTGSFSKNF 298
EQLFK TGS++K+F
Sbjct: 293 EQLFKETGSYNKSF 334
Score = 62.9 bits (131), Expect(2) = 3e-029
Identities = 26/54 (48%), Positives = 31/54 (57%)
Frame = +2 / +2
Query: 332 LTRAPSKLSSAFSGTXDKXAACQKTXYPXXXLTLXXXXYHKXXXKCSXXXXXLT 493
LTR+PSK + FSGT DK A C KT YP +T+ YHK KCS +T
Sbjct: 383 LTRSPSKAARMFSGTQDKCATCGKTAYPLEKVTVEEKAYHKSCFKCSHGGCAIT 544
>gb|CW376891.1|CW376891 fsbb001f054p19k0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f054p19, DNA
sequence
Length = 483
Score = 90.0 bits (190), Expect(2) = 7e-027
Identities = 34/48 (70%), Positives = 35/48 (72%)
Frame = +2 / -1
Query: 65 GTMSFTGXQDKCTXCDKTXHFIDLLTXXXXIYHKTCXXXXHCKGXXSM 208
GTMSFTG QDKCT CDKT HFIDLLT IYHKTC HCKG S+
Sbjct: 357 GTMSFTGTQDKCTACDKTVHFIDLLTADGVIYHKTCFKCSHCKGILSV 214
Score = 51.9 bits (107), Expect(2) = 7e-027
Identities = 19/25 (76%), Positives = 19/25 (76%)
Frame = +2 / -1
Query: 188 CKGXXSMCSYSSMDXXLYCKTHFEQ 262
C G MCSYSSMD LYCKTHFEQ
Sbjct: 75 CVGRLQMCSYSSMDGVLYCKTHFEQ 1
>gb|AW287022.2|AW287022 LG1_264_E07.b1_A002 Light Grown 1 (LG1) Sorghum bicolor cDNA, mRNA
sequence
Length = 511
Score = 93.6 bits (198), Expect(2) = 3e-026
Identities = 34/74 (45%), Positives = 47/74 (63%)
Frame = +2 / +2
Query: 77 FTGXQDKCTXCDKTXHFIDLLTXXXXIYHKTCXXXXHCKGXXSMCSYSSMDXXLYCKTHF 256
F+G Q KC C KT + +D L+ +H++C HCK S+ +YSS + YCK HF
Sbjct: 149 FSGTQQKCKVCTKTVYPMDQLSTDGVAFHRSCFKCQHCKSTLSLSNYSSFEGVPYCKAHF 328
Query: 257 EQLFKXTGSFSKNF 298
EQLFK TGS++K+F
Sbjct: 329 EQLFKETGSYNKSF 370
Score = 45.5 bits (93), Expect(2) = 3e-026
Identities = 18/28 (64%), Positives = 20/28 (71%)
Frame = +2 / +2
Query: 332 LTRAPSKLSSAFSGTXDKXAACQKTXYP 415
LTR+PSK + FSGT DK A C KT YP
Sbjct: 419 LTRSPSKAARMFSGTQDKCATCGKTAYP 502
>gb|CW497105.1|CW497105 fsbb001f289k18f0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f289k18, DNA
sequence
Length = 711
Score = 80.3 bits (169), Expect(2) = 2e-025
Identities = 31/39 (79%), Positives = 31/39 (79%)
Frame = +2 / +3
Query: 188 CKGXXSMCSYSSMDXXLYCKTHFEQLFKXTGSFSKNFTP 304
C G MCSYSSMD LYCKTHFEQLFK TGSFSK FTP
Sbjct: 156 CVGRLQMCSYSSMDGVLYCKTHFEQLFKETGSFSKKFTP 272
Score = 70.7 bits (148), Expect(2) = 3e-022
Identities = 30/43 (69%), Positives = 31/43 (72%)
Frame = -3 / -3
Query: 303 GVKFLEKLPVXLKSCSKWVLQYXXXSMEE*LHIEXXPLQWXXL 175
GV FLEKLPV LKSCSKWVLQY SMEE*LHI P + L
Sbjct: 271 GVNFLEKLPVSLKSCSKWVLQYSTPSMEE*LHICSRPTHYTSL 143
Score = 57.0 bits (118), Expect(2) = 2e-025
Identities = 22/27 (81%), Positives = 23/27 (85%)
Frame = +1 / +1
Query: 337 KGPKQAVVCIFWYPX*VRSMPENXVPA 417
KGPKQA+VCIFWY *V SMPEN VPA
Sbjct: 520 KGPKQAIVCIFWYSG*VCSMPENSVPA 600
Score = 56.1 bits (116), Expect(2) = 3e-022
Identities = 21/27 (77%), Positives = 21/27 (77%)
Frame = -2 / -3
Query: 415 RVXXFLACCXLIXGTRKCRRQLAWGPC 335
RV FLACC LI TRKCRR LAWGPC
Sbjct: 598 RVHCFLACCTLILSTRKCRR*LAWGPC 518
>gb|CN127798.1|CN127798 RHOH1_25_D05.b1_A002 Acid- and alkaline-treated roots Sorghum
bicolor cDNA clone RHOH1_25_D05_A002 3', mRNA sequence
Length = 720
Score = 70.3 bits (147), Expect(2) = 2e-024
Identities = 24/47 (51%), Positives = 33/47 (70%)
Frame = +2 / +2
Query: 158 YHKTCXXXXHCKGXXSMCSYSSMDXXLYCKTHFEQLFKXTGSFSKNF 298
+H++C HCK S+ +YSS + YCK HFEQLFK TGS++K+F
Sbjct: 8 FHRSCFKCQHCKSTLSLSNYSSFEGVPYCKAHFEQLFKETGSYNKSF 148
Score = 62.9 bits (131), Expect(2) = 2e-024
Identities = 26/54 (48%), Positives = 31/54 (57%)
Frame = +2 / +2
Query: 332 LTRAPSKLSSAFSGTXDKXAACQKTXYPXXXLTLXXXXYHKXXXKCSXXXXXLT 493
LTR+PSK + FSGT DK A C KT YP +T+ YHK KCS +T
Sbjct: 197 LTRSPSKAARMFSGTQDKCATCGKTAYPLEKVTVEEKAYHKSCFKCSHGGCAIT 358
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 382,304,153
Number of Sequences: 832831
Number of extensions: 3362451
Number of successful extensions: 47781
Number of sequences better than 1.0e-020: 13
length of database: 163,786,556
effective HSP length: 53
effective length of database: 119,646,513
effective search space used: 13280762943
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)