TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAI15h08.yg.2.1
(557 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BI245888.1|BI245888 IP1_64_G09.b1_A002 Immature pannicle... 137 1e-054
gb|CW033103.1|CW033103 104_262_10501765_115_30362 Sorghum m... 132 2e-037
gb|BI075220.1|BI075220 IP1_17_E11.b1_A002 Immature pannicle... 155 5e-037
gb|CL172808.1|CL172808 104_375_10890425_148_31796_329 Sorgh... 116 3e-025
>gb|BI245888.1|BI245888 IP1_64_G09.b1_A002 Immature pannicle 1 (IP1) Sorghum bicolor cDNA,
mRNA sequence
Length = 448
Score = 137 bits (293), Expect(3) = 1e-054
Identities = 57/80 (71%), Positives = 64/80 (80%)
Frame = -2 / +1
Query: 400 LMSNHLGVVLTFPVYHADLPSDAKEEDRVAATAGYLGGAFDVESLVENLLRQLAGNQELV 221
L SNHLGVVLTF VY DLP+DA E RV AT GYLGG FDVESLVENLL +LAGNQ++V
Sbjct: 142 LGSNHLGVVLTFAVYRPDLPADASVEQRVEATIGYLGGGFDVESLVENLLSKLAGNQDIV 321
Query: 220 VNVYDVTNISNPLVMYGSEV 161
VNVYDVTN S P+ +YG +
Sbjct: 322 VNVYDVTNASEPMALYGPSI 381
Score = 92.7 bits (196), Expect(3) = 2e-023
Identities = 44/78 (56%), Positives = 50/78 (64%)
Frame = +3 / -3
Query: 171 PYMTRGFEMFVXXXXXXXXXXLPASCLNKFSTNDSTSNAPPRYPAVAATRSSSLASEGRS 350
PY G E FV LPAS L+KFST+DSTSN PPRYP VA+TR S+ AS GRS
Sbjct: 371 PYKAIGSEAFVTS*TLTTIS*LPASLLSKFSTSDSTSNPPPRYPIVASTRCSTDASAGRS 192
Query: 351 A**TGKVNTTPR*FDMSR 404
T KV TTP+ FD S+
Sbjct: 191 GRYTAKVRTTPKWFDPSK 138
Score = 80.3 bits (169), Expect(3) = 1e-054
Identities = 30/48 (62%), Positives = 41/48 (85%)
Frame = -2 / +1
Query: 529 PVVYSQETVXYIEGLDMMXGEEDRENILRSRASGKAVLTRPFRLMSNH 386
PV++SQ+TV Y+ +DMM GEED+ENILR+R +GKAVLT PFRL+ ++
Sbjct: 10 PVIFSQDTVSYLARIDMMSGEEDQENILRARTTGKAVLTNPFRLLGSN 153
Score = 35.4 bits (71), Expect(3) = 1e-054
Identities = 11/14 (78%), Positives = 13/14 (92%)
Frame = -2 / +1
Query: 136 HICMLDFGDPFRKH 95
H+ MLDFGDPFR+H
Sbjct: 403 HVSMLDFGDPFRRH 444
Score = 29.0 bits (57), Expect(3) = 2e-023
Identities = 10/14 (71%), Positives = 11/14 (78%)
Frame = +3 / -3
Query: 96 CFLNGSPKSSMQMC 137
C LNGSPKS+M C
Sbjct: 443 CLLNGSPKSNMLTC 402
Score = 25.8 bits (50), Expect(3) = 2e-023
Identities = 16/43 (37%), Positives = 17/43 (39%)
Frame = +3 / -3
Query: 402 RNGRVRTAFPDALDLKIFXXXXXXXXXXXXXXXXTVSCEYTTG 530
RNG V TA P L K+F TVS E TG
Sbjct: 137 RNGFVNTALPVVLARKMFSWSSSPDIMSMRAR*ETVS*EKITG 9
>gb|CW033103.1|CW033103 104_262_10501765_115_30362 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 10501765, DNA
sequence
Length = 630
Score = 132 bits (283), Expect(2) = 2e-037
Identities = 51/56 (91%), Positives = 53/56 (94%)
Frame = +1 / +2
Query: 301 LRWQQHDPPPWHQKEDPHDRQEKSIRHPGDST*AGMVE*ELPSPMLLTSKYSHGPP 468
L+WQQH PPPWHQ+EDPHDRQEKSIRHPGDST*AGMV *ELPSPMLLTSK SHGPP
Sbjct: 170 LQWQQHVPPPWHQEEDPHDRQEKSIRHPGDST*AGMVS*ELPSPMLLTSKCSHGPP 337
Score = 127 bits (271), Expect(2) = 1e-034
Identities = 53/57 (92%), Positives = 55/57 (96%)
Frame = -2 / -2
Query: 469 EEDRENILRSRASGKAVLTRPFRLMSNHLGVVLTFPVYHADLPSDAKEEDRVAATAG 299
+EDRENILRSRASGKAVLTRPFRLMSNHLGVVLTFPVYH DLP DAKEE+RVAATAG
Sbjct: 338 QEDRENILRSRASGKAVLTRPFRLMSNHLGVVLTFPVYHVDLPPDAKEEERVAATAG 168
Score = 125 bits (268), Expect(2) = 4e-034
Identities = 50/56 (89%), Positives = 52/56 (92%)
Frame = +2 / +3
Query: 302 CGGSNTILLLGIRRKIRMIDRKSQYDTQVIRHEPEWSSENCLPRCS*PQNILTVLL 469
C GSNT LLLGIRRKI MIDRKSQYDTQVIRHEPEWS +NCLPRCS*PQN+LTVLL
Sbjct: 171 CSGSNTFLLLGIRRKIHMIDRKSQYDTQVIRHEPEWSRKNCLPRCS*PQNVLTVLL 338
Score = 113 bits (242), Expect = 2e-024
Identities = 50/56 (89%), Positives = 50/56 (89%)
Frame = -3 / -3
Query: 468 RRTVRIF*GQEHRGRQFSLDHSGSCRITWVSY*LFLSIMRIFLLMPRRRIVLLPPQ 301
RRTVR F*GQEHRGRQF DHSGSCRITWVSY*LFLSIM IFLLMPRRR VLLP Q
Sbjct: 337 RRTVRTF*GQEHRGRQFLRDHSGSCRITWVSY*LFLSIMWIFLLMPRRRNVLLPLQ 170
Score = 106 bits (226), Expect(2) = 9e-024
Identities = 48/53 (90%), Positives = 50/53 (94%)
Frame = +3 / +1
Query: 297 YPAVAATRSSSLASEGRSA**TGKVNTTPR*FDMSRNGRVRTAFPDALDLKIF 455
+PAVAATRSSSLAS GRS **TGKVNTTPR*FDMSRNG VRTAFPDALDLK+F
Sbjct: 166 HPAVAATRSSSLASGGRST**TGKVNTTPR*FDMSRNGLVRTAFPDALDLKMF 324
Score = 79.0 bits (166), Expect(2) = 3e-024
Identities = 31/34 (91%), Positives = 33/34 (97%)
Frame = -1 / -1
Query: 467 GGP*EYFEVKSIGEGSSHSTIPAHVESPGCRIDF 366
GGP*E+FEVKSIGEGSS+ TIPAHVESPGCRIDF
Sbjct: 336 GGP*EHFEVKSIGEGSSYETIPAHVESPGCRIDF 235
Score = 53.8 bits (111), Expect(2) = 3e-024
Identities = 23/32 (71%), Positives = 25/32 (78%)
Frame = -2 / -3
Query: 535 YAPVVYSQETVXYIEGLDMMXGEEDRENILRS 440
YAPVVYSQETV YIEGLDMM GE + + RS
Sbjct: 505 YAPVVYSQETVSYIEGLDMMSGEVCGDVVSRS 410
Score = 44.6 bits (91), Expect(2) = 2e-037
Identities = 18/26 (69%), Positives = 20/26 (76%)
Frame = +1 / +1
Query: 466 PRXTSCRAPRCXXRSPASIQRARTCP 543
PR TSCRAP+C RSPAS + ART P
Sbjct: 436 PRRTSCRAPQCRRRSPASRRPARTHP 513
Score = 40.5 bits (82), Expect(2) = 1e-034
Identities = 18/26 (69%), Positives = 19/26 (73%)
Frame = -1 / -2
Query: 542 GQVRARCILAGDRXXHRGARHDVXRG 465
G VRA +LAGDR H GARHDV RG
Sbjct: 512 G*VRAGRLLAGDRLLH*GARHDVRRG 435
Score = 40.0 bits (81), Expect(2) = 4e-034
Identities = 17/23 (73%), Positives = 19/23 (82%)
Frame = +2 / +2
Query: 467 LAXHHVEPLDVXDGLLRVYNGRV 535
LA HHVEPL+V DGLLRV + RV
Sbjct: 437 LAGHHVEPLNVGDGLLRVDDRRV 505
Score = 25.8 bits (50), Expect(2) = 9e-024
Identities = 10/11 (90%), Positives = 10/11 (90%)
Frame = +3 / +3
Query: 504 TVSCEYTTGAY 536
TVSCE TTGAY
Sbjct: 474 TVSCE*TTGAY 506
>gb|BI075220.1|BI075220 IP1_17_E11.b1_A002 Immature pannicle 1 (IP1) Sorghum bicolor cDNA,
mRNA sequence
Length = 609
Score = 155 bits (333), Expect = 5e-037
Identities = 58/113 (51%), Positives = 84/113 (74%)
Frame = -2 / +2
Query: 535 YAPVVYSQETVXYIEGLDMMXGEEDRENILRSRASGKAVLTRPFRLMSNHLGVVLTFPVY 356
YAPV+++Q+ ++ D++ G +DR+N+LR+R SGK VLT PF+L++N LGV+ T+ VY
Sbjct: 194 YAPVIFAQDAYKHVISFDLLSGADDRDNVLRARESGKGVLTAPFKLLNNRLGVISTYAVY 373
Query: 355 HADLPSDAKEEDRVAATAGYLGGAFDVESLVENLLRQLAGNQELVVNVYDVTN 197
+LP +A +DR+ A GYLGG FD+E+LV+ L QLAG Q ++VNVYD TN
Sbjct: 374 KYELPPNATPQDRIQAAIGYLGGIFDIEALVDKSLHQLAGKQSIMVNVYDTTN 532
>gb|CL172808.1|CL172808 104_375_10890425_148_31796_329 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10890425, DNA
sequence
Length = 581
Score = 116 bits (248), Expect = 3e-025
Identities = 45/54 (83%), Positives = 48/54 (88%)
Frame = -1 / +3
Query: 305 RRIPWGSI*CRIISGKFVEAASWQSGIGGKCL*CHKHFEPSCHVWIRSSSWQPL 144
++IPWG I*CRIISGKFVEAA WQSGIGGKCL*CHK F+PSCHV I SSSW PL
Sbjct: 420 QQIPWGII*CRIISGKFVEAACWQSGIGGKCL*CHKQFKPSCHVGIGSSSWHPL 581
Score = 114 bits (243), Expect = 1e-024
Identities = 46/52 (88%), Positives = 47/52 (90%)
Frame = +1 / -2
Query: 145 RGCQEELLIHT*QEGSKCL*HHKHLPPIPDCQLAASTNFPLMILHQMLPQGI 300
RGCQEEL I T*QEG CL*HHKHLPPIPDCQ AASTNFPLMILHQM+PQGI
Sbjct: 580 RGCQEELPIPT*QEGLNCL*HHKHLPPIPDCQQAASTNFPLMILHQMIPQGI 425
Score = 111 bits (236), Expect = 1e-023
Identities = 45/52 (86%), Positives = 49/52 (94%)
Frame = -3 / +2
Query: 300 DTLGEHLM*NH*WKIC*GS*LAIRNWW*MFMMSQTFRTLLSCMDQKFLLATP 145
DTLG+HLM*NH*WKIC*GS LAIRNWW*MFMMSQT +TLLSC D+KFLLA+P
Sbjct: 425 DTLGDHLM*NH*WKIC*GSLLAIRNWW*MFMMSQTVQTLLSCRDRKFLLASP 580
Score = 108 bits (230), Expect = 8e-023
Identities = 47/51 (92%), Positives = 48/51 (94%)
Frame = -2 / +1
Query: 298 YLGGAFDVESLVENLLRQLAGNQELVVNVYDVTNISNPLVMYGSEVPLGNP 146
YLGG+FDVESLVENLLRQLAGNQELVVNVYDVTN SNPLVM GSEVPLG P
Sbjct: 427 YLGGSFDVESLVENLLRQLAGNQELVVNVYDVTNSSNPLVM*GSEVPLGIP 579
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 742,959,904
Number of Sequences: 832831
Number of extensions: 11966590
Number of successful extensions: 139214
Number of sequences better than 1.0e-020: 5
length of database: 163,786,556
effective HSP length: 53
effective length of database: 119,646,513
effective search space used: 15793339716
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)