TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF1g01.yg.2.1
(335 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CL170430.1|CL170430 104_371_10813804_116_31791_124 Sorgh... 164 4e-040
gb|BG559259.1|BG559259 RHIZ2_52_F03.b1_A003 Rhizome2 (RHIZ2... 163 1e-039
gb|CW473573.1|CW473573 fsbb001f229a03k0 Sorghum methylation... 112 1e-038
gb|BG054341.1|BG054341 OV2_3_A09.b1_A002 Ovary 2 (OV2) Sorg... 137 8e-032
gb|CW513921.1|CW513921 115_5_10512125_1_30022 Sorghum unfil... 114 6e-025
gb|CW883674.1|CW883674 Sorghum CISP Intron:I00_X00_F_PRSC1_... 104 6e-022
gb|CW884311.1|CW884311 Sorghum CISP Intron:I00_X00_F_PRSC1_... 104 6e-022
>gb|CL170430.1|CL170430 104_371_10813804_116_31791_124 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10813804, DNA
sequence
Length = 746
Score = 164 bits (353), Expect = 4e-040
Identities = 66/88 (75%), Positives = 75/88 (85%)
Frame = -2 / +1
Query: 265 KVRXXYXXPDVFDRIFXITRGGIXKASRVINISEDIYAGFNSTLRQGNITHHEYIQVGKG 86
KVR Y PDVFDR++ + RGGI KAS+ INISEDI+AGFN TLR GN+THHEYIQVGKG
Sbjct: 106 KVRMHYGHPDVFDRLWFLGRGGISKASKTINISEDIFAGFNCTLRGGNVTHHEYIQVGKG 285
Query: 85 RDVGLNQIALFEGKVAGGNGEQVLSRDV 2
RDVGLNQ+++FE KVA GNGEQ LSRDV
Sbjct: 286 RDVGLNQVSMFEAKVASGNGEQTLSRDV 369
Score = 120 bits (256), Expect = 1e-026
Identities = 50/80 (62%), Positives = 55/80 (68%)
Frame = +3 / -1
Query: 3 TSRLRTCSPLPPATFPSNSAIWFKPTSRPFPTWIYSWCVMFPWRKVELNPAYISSLILIT 182
TSRL+ CSPLP AT SN W PTS P PTWIYSWCV P R+V+L PA ISSL+LI
Sbjct: 368 TSRLKVCSPLPLATLASNIETWLSPTSLPLPTWIYSWCVTLPPRRVQLKPANISSLMLIV 189
Query: 183 RDAXXMPPRVXXNIRSKTSG 242
DA +PPR RSKTSG
Sbjct: 188 FDALLIPPRPKNQRRSKTSG 129
>gb|BG559259.1|BG559259 RHIZ2_52_F03.b1_A003 Rhizome2 (RHIZ2) Sorghum propinquum cDNA, mRNA
sequence
Length = 456
Score = 163 bits (350), Expect = 1e-039
Identities = 65/88 (73%), Positives = 75/88 (85%)
Frame = -2 / +3
Query: 265 KVRXXYXXPDVFDRIFXITRGGIXKASRVINISEDIYAGFNSTLRQGNITHHEYIQVGKG 86
+VR Y PDVFDR++ + RGGI KAS+ INISEDI+AGFN TLR GN+THHEYIQVGKG
Sbjct: 6 RVRMHYGHPDVFDRLWFLGRGGISKASKTINISEDIFAGFNCTLRGGNVTHHEYIQVGKG 185
Query: 85 RDVGLNQIALFEGKVAGGNGEQVLSRDV 2
RDVGLNQ+++FE KVA GNGEQ LSRDV
Sbjct: 186 RDVGLNQVSMFEAKVASGNGEQTLSRDV 269
Score = 120 bits (256), Expect = 1e-026
Identities = 50/80 (62%), Positives = 55/80 (68%)
Frame = +3 / -3
Query: 3 TSRLRTCSPLPPATFPSNSAIWFKPTSRPFPTWIYSWCVMFPWRKVELNPAYISSLILIT 182
TSRL+ CSPLP AT SN W PTS P PTWIYSWCV P R+V+L PA ISSL+LI
Sbjct: 268 TSRLKVCSPLPLATLASNIETWLSPTSLPLPTWIYSWCVTLPPRRVQLKPANISSLMLIV 89
Query: 183 RDAXXMPPRVXXNIRSKTSG 242
DA +PPR RSKTSG
Sbjct: 88 FDALLIPPRPKNQRRSKTSG 29
>gb|CW473573.1|CW473573 fsbb001f229a03k0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f229a03, DNA
sequence
Length = 646
Score = 112 bits (240), Expect(2) = 1e-038
Identities = 43/53 (81%), Positives = 51/53 (96%)
Frame = -2 / +3
Query: 160 IYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDV 2
+++GFNSTLR+GN+THHEYIQVGKGRDVGLNQI+LFE KVA GNGEQ+LSRD+
Sbjct: 486 LFSGFNSTLRRGNVTHHEYIQVGKGRDVGLNQISLFEAKVACGNGEQILSRDI 644
Score = 66.1 bits (138), Expect(2) = 1e-038
Identities = 28/39 (71%), Positives = 31/39 (79%)
Frame = -2 / +2
Query: 265 KVRXXYXXPDVFDRIFXITRGGIXKASRVINISEDIYAG 149
+VR Y PDVFDRIF ITRGGI KAS IN+SEDI+AG
Sbjct: 263 RVRFHYGHPDVFDRIFHITRGGISKASCGINLSEDIFAG 379
>gb|BG054341.1|BG054341 OV2_3_A09.b1_A002 Ovary 2 (OV2) Sorghum bicolor cDNA, mRNA sequence
Length = 528
Score = 137 bits (293), Expect = 8e-032
Identities = 58/64 (90%), Positives = 59/64 (92%)
Frame = -2 / +3
Query: 193 KASRVINISEDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVL 14
K R NISEDIYAGFNSTLRQG ITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVL
Sbjct: 306 KYRRETNISEDIYAGFNSTLRQGCITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVL 485
Query: 13 SRDV 2
SRD+
Sbjct: 486 SRDI 497
Score = 100 bits (213), Expect = 8e-021
Identities = 45/60 (75%), Positives = 47/60 (78%)
Frame = +3 / -3
Query: 6 SRLRTCSPLPPATFPSNSAIWFKPTSRPFPTWIYSWCVMFPWRKVELNPAYISSLILITR 185
SRLRTCSP PPATFPS SAI PTS P PT IYS VM P R VELNPAYISSLIL++R
Sbjct: 493 SRLRTCSPFPPATFPSKSAI*LSPTSLPLPT*IYS**VMHP*RSVELNPAYISSLILVSR 314
>gb|CW513921.1|CW513921 115_5_10512125_1_30022 Sorghum unfiltered library (LibID: 115)
Sorghum bicolor genomic clone 10512125, DNA sequence
Length = 639
Score = 114 bits (243), Expect = 6e-025
Identities = 45/58 (77%), Positives = 51/58 (87%)
Frame = -2 / +2
Query: 175 NISEDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDV 2
NI I AGFNSTLR+GN+THHEY+QVGKGRDVGLNQI+LFE K+A GNGEQ LSRD+
Sbjct: 38 NIVASIGAGFNSTLREGNVTHHEYMQVGKGRDVGLNQISLFEAKIANGNGEQTLSRDI 211
>gb|CW883674.1|CW883674 Sorghum CISP Intron:I00_X00_F_PRSC1_002_SORG_BTXX BTXX Sorghum
bicolor genomic, DNA sequence
Length = 509
Score = 104 bits (221), Expect = 6e-022
Identities = 40/50 (80%), Positives = 45/50 (90%)
Frame = -2 / +1
Query: 151 GFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDV 2
GFNSTLRQGN+THHEYIQ+GKGRDVG+NQI+ FE KVA GNGEQ L RD+
Sbjct: 64 GFNSTLRQGNVTHHEYIQLGKGRDVGMNQISNFEAKVANGNGEQTLCRDI 213
>gb|CW884311.1|CW884311 Sorghum CISP Intron:I00_X00_F_PRSC1_002_SORG_IS18 IS18 Sorghum
bicolor genomic, DNA sequence
Length = 501
Score = 104 bits (221), Expect = 6e-022
Identities = 40/50 (80%), Positives = 45/50 (90%)
Frame = -2 / +2
Query: 151 GFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDV 2
GFNSTLRQGN+THHEYIQ+GKGRDVG+NQI+ FE KVA GNGEQ L RD+
Sbjct: 56 GFNSTLRQGNVTHHEYIQLGKGRDVGMNQISNFEAKVANGNGEQTLCRDI 205
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 302,915,689
Number of Sequences: 832831
Number of extensions: 3835779
Number of successful extensions: 254281
Number of sequences better than 1.0e-020: 9
length of database: 163,786,556
effective HSP length: 51
effective length of database: 121,312,175
effective search space used: 7278730500
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)