TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF18e02.yg.3.1
(315 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BM324384.1|BM324384 PIC1_27_F06.b1_A002 Pathogen-infecte... 199 8e-051
gb|CD206409.1|CD206409 HS1_22_D02.g1_A012 Heat-shocked seed... 164 4e-040
gb|BM324288.1|BM324288 PIC1_26_E08.b1_A002 Pathogen-infecte... 155 1e-037
gb|CD431032.1|CD431032 ETH1_6_D11.g1_A002 Ethylene-treated ... 79 2e-014
gb|CL193734.1|CL193734 104_417_10941140_114_32274_070 Sorgh... 45 4e-004
gb|CL193735.1|CL193735 104_417_10941140_116_32279_070 Sorgh... 45 4e-004
gb|CL698098.2|CL698098 SP__Ba0024O08.r SP__Ba Sorghum propi... 45 4e-004
gb|CL696930.1|CL696930 SP__Ba0003I23.r SP__Ba Sorghum propi... 45 5e-004
gb|CW477192.1|CW477192 fsbb001f234g14k0 Sorghum methylation... 25 1.3
gb|CW347611.1|CW347611 fsbb001f007j14k0 Sorghum methylation... 25 1.5
gb|CW240825.1|CW240825 104_699_11218426_148_37537_035 Sorgh... 25 1.6
gb|CW240824.1|CW240824 104_699_11218426_116_37550_035 Sorgh... 25 1.6
gb|CW450130.1|CW450130 fsbb001f188j18k0 Sorghum methylation... 32 3.5
gb|CW324018.1|CW324018 104_818_11476935_116_35904_058 Sorgh... 25 5.4
gb|CW456179.1|CW456179 fsbb001f201g16f0 Sorghum methylation... 25 6.0
gb|CF758854.1|CF758854 DSAF1_37_D05.g1_A011 Drought-stresse... 31 6.6
gb|BG273626.1|BG273626 OV2_28_A03.b1_A002 Ovary 2 (OV2) Sor... 25 8.6
gb|BZ352165.1|BZ352165 hm86e05.g1 WGS-SbicolorF (JM107 adap... 30 9.1
gb|CW246474.1|CW246474 104_707_11221355_116_35082_042 Sorgh... 30 9.1
gb|CW397356.1|CW397356 fsbb001f086d19k0 Sorghum methylation... 30 9.1
>gb|BM324384.1|BM324384 PIC1_27_F06.b1_A002 Pathogen-infected compatible 1 (PIC1) Sorghum
bicolor cDNA, mRNA sequence
Length = 580
Score = 199 bits (430), Expect = 8e-051
Identities = 85/101 (84%), Positives = 87/101 (86%)
Frame = +2 / +2
Query: 2 SLRANCCSWWIISAITTATPYFIFFH*FICKARGYGSQVLFSGDNTK*RQYTFQDYFCYC 181
SLRANCCSWWIISAITTAT +FIFF *FI KA GYGSQVLF GDNTK* QYTFQD CY
Sbjct: 278 SLRANCCSWWIISAITTATSHFIFFR*FIRKACGYGSQVLFIGDNTK*SQYTFQDSICYS 457
Query: 182 EDHSERC*GKESFI*SPTILPIVYKLAL*PYYY*WPS*WF* 304
E+HSERC*GKESFI*S IL IVYKLAL*PYYY WP *WF*
Sbjct: 458 ENHSERC*GKESFI*SSAILSIVYKLAL*PYYYRWPP*WF* 580
Score = 193 bits (417), Expect = 5e-049
Identities = 83/100 (83%), Positives = 86/100 (86%)
Frame = +3 / +3
Query: 3 VSEQIVAXXXXXXXXXXXXHISYFSIDSYAKLVVMVLKYSSVEITPNKGSILSKIISVTV 182
VSEQIVA HISYFS+DSYAKLVVMVLKYSS+EITPNK SILSKI+SVTV
Sbjct: 279 VSEQIVAPGGSSQQSPQQPHISYFSVDSYAKLVVMVLKYSSLEITPNKASILSKILSVTV 458
Query: 183 RTIQKDAEEKKASFNPRPYFRLXINWLYDLTTTDGHHDGS 302
RTIQKDAEEKKASFNPRPYFRL INWLYDLTTTDGHHDGS
Sbjct: 459 RTIQKDAEEKKASFNPRPYFRLFINWLYDLTTTDGHHDGS 578
Score = 159 bits (342), Expect = 1e-038
Identities = 78/101 (77%), Positives = 81/101 (80%)
Frame = -1 / -2
Query: 303 *NHHDGHQ***GHRANLXTIGSMVGD*MKLSFPQHLSEXXXXXXXXXXKVYCLYLVLSPL 124
*NHH GH+***GHRANL TI SM D*MKLSFPQHLSE KVY LYLVLSP+
Sbjct: 579 *NHHGGHR***GHRANL*TIESMAED*MKLSFPQHLSEWFSL*QIESWKVYWLYLVLSPM 400
Query: 123 KST*EP*PRALHMNQWKNMKYGVAVVIAEMIHQEQQFARRL 1
KST*EP*P+AL MNQ KNMK VAVVIAEMIHQEQQFARRL
Sbjct: 399 KST*EP*PQALRMNQRKNMKCEVAVVIAEMIHQEQQFARRL 277
Score = 159 bits (342), Expect(2) = 8e-039
Identities = 67/81 (82%), Positives = 72/81 (88%)
Frame = -2 / -3
Query: 302 RTIMMAISSSKVIEPIYXQSEVWSGIK*SFLFLSIFLNGPHSNRNNLGKYTAFIWCYLH* 123
RTIM+AI SSKVIEPIY QS+VW IK*SFLFLSIFLNG H NR NLGKYT FIWCYL *
Sbjct: 578 RTIMVAIGSSKVIEPIYKQSKVWPRIK*SFLFLSIFLNGSHCNR*NLGKYTGFIWCYLQ* 399
Query: 122 RVLENHNHELCI*INGKI*NM 60
RVLENHNH+LC+*INGKI*N+
Sbjct: 398 RVLENHNHKLCV*INGKI*NV 336
Score = 144 bits (309), Expect = 4e-034
Identities = 66/81 (81%), Positives = 68/81 (83%)
Frame = -3 / -1
Query: 304 LEPS*WPXXXXXXXSQFIXNRKYGRGLNEAFFSSASF*MVLTVTEIILESILPLFGVIST 125
LEPS WP SQFI NRKYGRGLNEAFFSSASF*MVLTVT+ ILESIL LFGVIS
Sbjct: 580 LEPSWWPSVVVRS*SQFINNRKYGRGLNEAFFSSASF*MVLTVTDRILESILALFGVISN 401
Query: 124 EEYLRTITTSFAYESMEKYEI 62
EEYLRTITTSFAYES EKYE+
Sbjct: 400 EEYLRTITTSFAYESTEKYEM 338
Score = 113 bits (242), Expect(2) = 2e-032
Identities = 46/52 (88%), Positives = 46/52 (88%)
Frame = +1 / +1
Query: 1 *SPSKLLLLVDHLSNHHSNPIFHIFPLIHMQSSWLWFSSTLQWR*HQIKAVY 156
*SPSKLLLLVDHLSNHHSN FHIFPLIH QS WLWFSSTL WR*HQIK VY
Sbjct: 277 *SPSKLLLLVDHLSNHHSNLTFHIFPLIHTQSLWLWFSSTLHWR*HQIKPVY 432
Score = 44.1 bits (90), Expect(2) = 2e-032
Identities = 19/23 (82%), Positives = 19/23 (82%)
Frame = +1 / +1
Query: 196 KMLRKRKLHLIPDHTSDCX*IGS 264
KMLRKRKLHLI HT DC *IGS
Sbjct: 472 KMLRKRKLHLILGHTFDCL*IGS 540
Score = 19.4 bits (36), Expect(2) = 8e-039
Identities = 7/7 (100%), Positives = 7/7 (100%)
Frame = -3 / -1
Query: 22 ATICSET 2
ATICSET
Sbjct: 298 ATICSET 278
>gb|CD206409.1|CD206409 HS1_22_D02.g1_A012 Heat-shocked seedlings Sorghum bicolor cDNA
clone HS1_22_D02_A012 5', mRNA sequence
Length = 589
Score = 164 bits (353), Expect = 4e-040
Identities = 67/72 (93%), Positives = 70/72 (97%)
Frame = +3 / +2
Query: 99 VVMVLKYSSVEITPNKGSILSKIISVTVRTIQKDAEEKKASFNPRPYFRLXINWLYDLTT 278
VVMVLKYSS+EITPNK SILSKI+SVTVRTIQKDAEEKKASFNPRPYFRL INWLYDLTT
Sbjct: 2 VVMVLKYSSLEITPNKASILSKILSVTVRTIQKDAEEKKASFNPRPYFRLFINWLYDLTT 181
Query: 279 TDGHHDGSNFQV 314
TDGHHDGSNFQ+
Sbjct: 182 TDGHHDGSNFQI 217
Score = 144 bits (310), Expect = 3e-034
Identities = 61/72 (84%), Positives = 63/72 (87%)
Frame = -2 / -1
Query: 314 NLKIRTIMMAISSSKVIEPIYXQSEVWSGIK*SFLFLSIFLNGPHSNRNNLGKYTAFIWC 135
NLKIRTIM+AI SSKVIEPIY QS+VW IK*SFLFLSIFLNG H NR NLGKYT FIWC
Sbjct: 217 NLKIRTIMVAIGSSKVIEPIYKQSKVWPRIK*SFLFLSIFLNGSHCNR*NLGKYTGFIWC 38
Query: 134 YLH*RVLENHNH 99
YL *RVLENHNH
Sbjct: 37 YLQ*RVLENHNH 2
Score = 138 bits (297), Expect = 2e-032
Identities = 59/70 (84%), Positives = 60/70 (85%)
Frame = +2 / +1
Query: 101 GYGSQVLFSGDNTK*RQYTFQDYFCYCEDHSERC*GKESFI*SPTILPIVYKLAL*PYYY 280
GYGSQVLF GDNTK* QYTFQD CY E+HSERC*GKESFI*S IL IVYKLAL*PYYY
Sbjct: 4 GYGSQVLFIGDNTK*SQYTFQDSICYSENHSERC*GKESFI*SSAILSIVYKLAL*PYYY 183
Query: 281 *WPS*WF*FS 310
WP *WF*FS
Sbjct: 184 RWPP*WF*FS 213
Score = 123 bits (263), Expect = 9e-028
Identities = 58/71 (81%), Positives = 59/71 (83%)
Frame = -3 / -2
Query: 310 *KLEPS*WPXXXXXXXSQFIXNRKYGRGLNEAFFSSASF*MVLTVTEIILESILPLFGVI 131
*KLEPS WP SQFI NRKYGRGLNEAFFSSASF*MVLTVT+ ILESIL LFGVI
Sbjct: 213 *KLEPSWWPSVVVRS*SQFINNRKYGRGLNEAFFSSASF*MVLTVTDRILESILALFGVI 34
Query: 130 STEEYLRTITT 98
S EEYLRTITT
Sbjct: 33 SNEEYLRTITT 1
Score = 107 bits (228), Expect = 6e-023
Identities = 53/72 (73%), Positives = 55/72 (76%)
Frame = -1 / -3
Query: 315 KPEN*NHHDGHQ***GHRANLXTIGSMVGD*MKLSFPQHLSEXXXXXXXXXXKVYCLYLV 136
K EN*NHH GH+***GHRANL TI SM D*MKLSFPQHLSE KVY LYLV
Sbjct: 218 KSEN*NHHGGHR***GHRANL*TIESMAED*MKLSFPQHLSEWFSL*QIESWKVYWLYLV 39
Query: 135 LSPLKST*EP*P 100
LSP+KST*EP*P
Sbjct: 38 LSPMKST*EP*P 3
Score = 48.7 bits (100), Expect(2) = 3e-014
Identities = 23/40 (57%), Positives = 23/40 (57%)
Frame = +1 / +3
Query: 196 KMLRKRKLHLIPDHTSDCX*IGSXXXXXXXXXXXXXIFRF 315
KMLRKRKLHLI HT DC *IGS IFRF
Sbjct: 99 KMLRKRKLHLILGHTFDCL*IGSMTLLLPMATMMVLIFRF 218
Score = 47.8 bits (98), Expect(2) = 3e-014
Identities = 17/19 (89%), Positives = 17/19 (89%)
Frame = +1 / +3
Query: 100 WLWFSSTLQWR*HQIKAVY 156
WLWFSSTL WR*HQIK VY
Sbjct: 3 WLWFSSTLHWR*HQIKPVY 59
>gb|BM324288.1|BM324288 PIC1_26_E08.b1_A002 Pathogen-infected compatible 1 (PIC1) Sorghum
bicolor cDNA, mRNA sequence
Length = 515
Score = 155 bits (334), Expect = 1e-037
Identities = 66/79 (83%), Positives = 68/79 (86%)
Frame = +2 / +2
Query: 2 SLRANCCSWWIISAITTATPYFIFFH*FICKARGYGSQVLFSGDNTK*RQYTFQDYFCYC 181
SLRANCCSWWIISAITTAT +FIFF *FI KA GYGSQVLF GDNTK* QYTFQD CY
Sbjct: 278 SLRANCCSWWIISAITTATSHFIFFR*FIRKACGYGSQVLFIGDNTK*SQYTFQDSICYS 457
Query: 182 EDHSERC*GKESFI*SPTI 238
E+HSERC*GKESFI*S I
Sbjct: 458 ENHSERC*GKESFI*SSAI 514
Score = 141 bits (302), Expect = 4e-033
Identities = 63/79 (79%), Positives = 66/79 (83%)
Frame = +3 / +3
Query: 3 VSEQIVAXXXXXXXXXXXXHISYFSIDSYAKLVVMVLKYSSVEITPNKGSILSKIISVTV 182
VSEQIVA HISYFS+DSYAKLVVMVLKYSS+EITPNK SILSKI+SVTV
Sbjct: 279 VSEQIVAPGGSSQQSPQQPHISYFSVDSYAKLVVMVLKYSSLEITPNKASILSKILSVTV 458
Query: 183 RTIQKDAEEKKASFNPRPY 239
RTIQKDAEEKKASFNPRPY
Sbjct: 459 RTIQKDAEEKKASFNPRPY 515
Score = 122 bits (262), Expect = 1e-027
Identities = 60/79 (75%), Positives = 62/79 (78%)
Frame = -1 / -3
Query: 237 MVGD*MKLSFPQHLSEXXXXXXXXXXKVYCLYLVLSPLKST*EP*PRALHMNQWKNMKYG 58
M D*MKLSFPQHLSE KVY LYLVLSP+KST*EP*P+AL MNQ KNMK
Sbjct: 513 MAED*MKLSFPQHLSEWFSL*QIESWKVYWLYLVLSPMKST*EP*PQALRMNQRKNMKCE 334
Query: 57 VAVVIAEMIHQEQQFARRL 1
VAVVIAEMIHQEQQFARRL
Sbjct: 333 VAVVIAEMIHQEQQFARRL 277
Score = 122 bits (261), Expect(2) = 8e-028
Identities = 50/60 (83%), Positives = 53/60 (88%)
Frame = -2 / -1
Query: 239 VWSGIK*SFLFLSIFLNGPHSNRNNLGKYTAFIWCYLH*RVLENHNHELCI*INGKI*NM 60
VW IK*SFLFLSIFLNG H NR NLGKYT FIWCYL *RVLENHNH+LC+*INGKI*N+
Sbjct: 515 VWPRIK*SFLFLSIFLNGSHCNR*NLGKYTGFIWCYLQ*RVLENHNHKLCV*INGKI*NV 336
Score = 118 bits (253), Expect = 2e-026
Identities = 53/59 (89%), Positives = 55/59 (93%)
Frame = -3 / -2
Query: 238 YGRGLNEAFFSSASF*MVLTVTEIILESILPLFGVISTEEYLRTITTSFAYESMEKYEI 62
YGRGLNEAFFSSASF*MVLTVT+ ILESIL LFGVIS EEYLRTITTSFAYES EKYE+
Sbjct: 514 YGRGLNEAFFSSASF*MVLTVTDRILESILALFGVISNEEYLRTITTSFAYESTEKYEM 338
Score = 113 bits (242), Expect(2) = 3e-028
Identities = 46/52 (88%), Positives = 46/52 (88%)
Frame = +1 / +1
Query: 1 *SPSKLLLLVDHLSNHHSNPIFHIFPLIHMQSSWLWFSSTLQWR*HQIKAVY 156
*SPSKLLLLVDHLSNHHSN FHIFPLIH QS WLWFSSTL WR*HQIK VY
Sbjct: 277 *SPSKLLLLVDHLSNHHSNLTFHIFPLIHTQSLWLWFSSTLHWR*HQIKPVY 432
Score = 29.5 bits (58), Expect(2) = 3e-028
Identities = 12/14 (85%), Positives = 12/14 (85%)
Frame = +1 / +1
Query: 196 KMLRKRKLHLIPDH 237
KMLRKRKLHLI H
Sbjct: 472 KMLRKRKLHLILGH 513
Score = 19.4 bits (36), Expect(2) = 8e-028
Identities = 7/7 (100%), Positives = 7/7 (100%)
Frame = -3 / -2
Query: 22 ATICSET 2
ATICSET
Sbjct: 298 ATICSET 278
>gb|CD431032.1|CD431032 ETH1_6_D11.g1_A002 Ethylene-treated seedlings Sorghum bicolor cDNA
clone ETH1_6_D11_A002 5', mRNA sequence
Length = 816
Score = 79.4 bits (167), Expect = 2e-014
Identities = 28/30 (93%), Positives = 29/30 (96%)
Frame = +3 / +1
Query: 225 NPRPYFRLXINWLYDLTTTDGHHDGSNFQV 314
NPRPYFRL INWLYDLTTTDGHHDGSNFQ+
Sbjct: 1 NPRPYFRLFINWLYDLTTTDGHHDGSNFQI 90
Score = 56.1 bits (116), Expect = 2e-007
Identities = 24/30 (80%), Positives = 26/30 (86%)
Frame = -2 / -1
Query: 314 NLKIRTIMMAISSSKVIEPIYXQSEVWSGI 225
NLKIRTIM+AI SSKVIEPIY QS+VW I
Sbjct: 90 NLKIRTIMVAIGSSKVIEPIYKQSKVWPRI 1
Score = 54.7 bits (113), Expect = 4e-007
Identities = 23/28 (82%), Positives = 23/28 (82%)
Frame = +2 / +3
Query: 227 SPTILPIVYKLAL*PYYY*WPS*WF*FS 310
S IL IVYKLAL*PYYY WP *WF*FS
Sbjct: 3 SSAILSIVYKLAL*PYYYRWPP*WF*FS 86
Score = 50.6 bits (104), Expect = 8e-006
Identities = 23/30 (76%), Positives = 24/30 (80%)
Frame = -1 / -3
Query: 315 KPEN*NHHDGHQ***GHRANLXTIGSMVGD 226
K EN*NHH GH+***GHRANL TI SM D
Sbjct: 91 KSEN*NHHGGHR***GHRANL*TIESMAED 2
Score = 42.3 bits (86), Expect = 0.002
Identities = 19/28 (67%), Positives = 19/28 (67%)
Frame = -3 / -2
Query: 310 *KLEPS*WPXXXXXXXSQFIXNRKYGRG 227
*KLEPS WP SQFI NRKYGRG
Sbjct: 86 *KLEPSWWPSVVVRS*SQFINNRKYGRG 3
>gb|CL193734.1|CL193734 104_417_10941140_114_32274_070 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10941140, DNA
sequence
Length = 727
Score = 45.1 bits (92), Expect = 4e-004
Identities = 22/39 (56%), Positives = 27/39 (69%)
Frame = +1 / +2
Query: 10 SKLLLLVDHLSNHHSNPIFHIFPLIHMQSSWLWFSSTLQ 126
SKLLLLVD+L +H SN F IF LIH+Q+ L S +Q
Sbjct: 296 SKLLLLVDYLHSHPSNTRFLIFQLIHIQNL*LQLSRYIQ 412
Score = 43.7 bits (89), Expect = 0.001
Identities = 19/42 (45%), Positives = 27/42 (64%)
Frame = +2 / +3
Query: 2 SLRANCCSWWIISAITTATPYFIFFH*FICKARGYGSQVLFS 127
S+ A+ CS W IS + ATP F+FF+*FI K + Q +F+
Sbjct: 288 SV*ASYCS*WTISTVIPATPDFLFFN*FIFKTCDFSCQGIFN 413
Score = 41.4 bits (84), Expect = 0.004
Identities = 20/41 (48%), Positives = 23/41 (56%)
Frame = +3 / +1
Query: 3 VSEQIVAXXXXXXXXXXXXHISYFSIDSYAKLVVMVLKYSS 125
VSEQ +A ISYFSIDSY+KLV V+K S
Sbjct: 289 VSEQAIAPSGLSPQSSQQHQISYFSIDSYSKLVTSVVKVYS 411
Score = 35.4 bits (71), Expect = 0.28
Identities = 16/33 (48%), Positives = 22/33 (66%)
Frame = -1 / -1
Query: 99 RALHMNQWKNMKYGVAVVIAEMIHQEQQFARRL 1
+ L+MNQ KN K GVA + E++H EQ A+ L
Sbjct: 385 QVLNMNQLKNKKSGVAGMTVEIVH*EQ*LAQTL 287
Score = 31.8 bits (63), Expect = 3.5
Identities = 14/22 (63%), Positives = 15/22 (68%)
Frame = -3 / -3
Query: 124 EEYLRTITTSFAYESMEKYEIW 59
E L T TSF YES+EK EIW
Sbjct: 410 EYTLTTEVTSFEYESIEK*EIW 345
>gb|CL193735.1|CL193735 104_417_10941140_116_32279_070 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10941140, DNA
sequence
Length = 641
Score = 45.1 bits (92), Expect = 4e-004
Identities = 22/39 (56%), Positives = 27/39 (69%)
Frame = +1 / -2
Query: 10 SKLLLLVDHLSNHHSNPIFHIFPLIHMQSSWLWFSSTLQ 126
SKLLLLVD+L +H SN F IF LIH+Q+ L S +Q
Sbjct: 610 SKLLLLVDYLHSHPSNTRFLIFQLIHIQNL*LQLSRYIQ 494
Score = 43.7 bits (89), Expect = 0.001
Identities = 19/42 (45%), Positives = 27/42 (64%)
Frame = +2 / -3
Query: 2 SLRANCCSWWIISAITTATPYFIFFH*FICKARGYGSQVLFS 127
S+ A+ CS W IS + ATP F+FF+*FI K + Q +F+
Sbjct: 618 SV*ASYCS*WTISTVIPATPDFLFFN*FIFKTCDFSCQGIFN 493
Score = 41.4 bits (84), Expect = 0.004
Identities = 20/41 (48%), Positives = 23/41 (56%)
Frame = +3 / -1
Query: 3 VSEQIVAXXXXXXXXXXXXHISYFSIDSYAKLVVMVLKYSS 125
VSEQ +A ISYFSIDSY+KLV V+K S
Sbjct: 617 VSEQAIAPSGLSPQSSQQHQISYFSIDSYSKLVTSVVKVYS 495
Score = 35.4 bits (71), Expect = 0.28
Identities = 16/33 (48%), Positives = 22/33 (66%)
Frame = -1 / +2
Query: 99 RALHMNQWKNMKYGVAVVIAEMIHQEQQFARRL 1
+ L+MNQ KN K GVA + E++H EQ A+ L
Sbjct: 521 QVLNMNQLKNKKSGVAGMTVEIVH*EQ*LAQTL 619
Score = 31.8 bits (63), Expect = 3.5
Identities = 14/22 (63%), Positives = 15/22 (68%)
Frame = -3 / +1
Query: 124 EEYLRTITTSFAYESMEKYEIW 59
E L T TSF YES+EK EIW
Sbjct: 496 EYTLTTEVTSFEYESIEK*EIW 561
>gb|CL698098.2|CL698098 SP__Ba0024O08.r SP__Ba Sorghum propinquum genomic clone
SP__Ba0024O08 3', DNA sequence
Length = 662
Score = 45.1 bits (92), Expect = 4e-004
Identities = 17/35 (48%), Positives = 24/35 (68%)
Frame = -2 / -2
Query: 218 SFLFLSIFLNGPHSNRNNLGKYTAFIWCYLH*RVL 114
++ ++ +F + + NLGKYT FIWCYL *RVL
Sbjct: 640 NYKYMYMFQSHNQFVKTNLGKYTGFIWCYLQ*RVL 536
Score = 38.2 bits (77), Expect = 0.041
Identities = 14/23 (60%), Positives = 21/23 (91%)
Frame = +3 / +2
Query: 99 VVMVLKYSSVEITPNKGSILSKI 167
+++ ++YSS+EITPNK SILSK+
Sbjct: 521 LLICIQYSSLEITPNKASILSKV 589
Score = 35.9 bits (72), Expect = 0.20
Identities = 16/23 (69%), Positives = 16/23 (69%)
Frame = +2 / +1
Query: 104 YGSQVLFSGDNTK*RQYTFQDYF 172
Y VLF GDNTK* QYTFQ F
Sbjct: 526 YLYSVLFIGDNTK*SQYTFQGSF 594
Score = 32.7 bits (65), Expect = 1.9
Identities = 14/16 (87%), Positives = 14/16 (87%)
Frame = -3 / -3
Query: 163 LESILPLFGVISTEEY 116
LESIL LFGVIS EEY
Sbjct: 585 LESILALFGVISNEEY 538
Score = 31.3 bits (62), Expect = 4.8
Identities = 12/14 (85%), Positives = 12/14 (85%)
Frame = +1 / +3
Query: 115 STLQWR*HQIKAVY 156
STL WR*HQIK VY
Sbjct: 537 STLHWR*HQIKPVY 578
>gb|CL696930.1|CL696930 SP__Ba0003I23.r SP__Ba Sorghum propinquum genomic clone
SP__Ba0003I23 3', DNA sequence
Length = 638
Score = 44.6 bits (91), Expect = 5e-004
Identities = 17/34 (50%), Positives = 23/34 (67%)
Frame = -2 / -2
Query: 215 FLFLSIFLNGPHSNRNNLGKYTAFIWCYLH*RVL 114
+ ++ +F + + NLGKYT FIWCYL *RVL
Sbjct: 637 YKYMYMFQSHNQFVKTNLGKYTGFIWCYLQ*RVL 536
Score = 38.2 bits (77), Expect = 0.041
Identities = 14/23 (60%), Positives = 21/23 (91%)
Frame = +3 / +2
Query: 99 VVMVLKYSSVEITPNKGSILSKI 167
+++ ++YSS+EITPNK SILSK+
Sbjct: 521 LLICIQYSSLEITPNKASILSKV 589
Score = 35.9 bits (72), Expect = 0.20
Identities = 16/23 (69%), Positives = 16/23 (69%)
Frame = +2 / +1
Query: 104 YGSQVLFSGDNTK*RQYTFQDYF 172
Y VLF GDNTK* QYTFQ F
Sbjct: 526 YLYSVLFIGDNTK*SQYTFQGSF 594
Score = 32.7 bits (65), Expect = 1.9
Identities = 14/16 (87%), Positives = 14/16 (87%)
Frame = -3 / -3
Query: 163 LESILPLFGVISTEEY 116
LESIL LFGVIS EEY
Sbjct: 585 LESILALFGVISNEEY 538
Score = 31.3 bits (62), Expect = 4.8
Identities = 12/14 (85%), Positives = 12/14 (85%)
Frame = +1 / +3
Query: 115 STLQWR*HQIKAVY 156
STL WR*HQIK VY
Sbjct: 537 STLHWR*HQIKPVY 578
>gb|CW477192.1|CW477192 fsbb001f234g14k0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f234g14, DNA
sequence
Length = 519
Score = 25.4 bits (49), Expect(2) = 1.3
Identities = 7/22 (31%), Positives = 13/22 (59%)
Frame = +1 / -2
Query: 73 FPLIHMQSSWLWFSSTLQWR*H 138
F +H + W+W + + WR*+
Sbjct: 257 FIYMHQKLMWMWKTRYIMWR*Y 192
Score = 24.0 bits (46), Expect(2) = 1.3
Identities = 7/16 (43%), Positives = 10/16 (62%)
Frame = +2 / -2
Query: 167 YFCYCEDHSERC*GKE 214
+FC + HS RC K+
Sbjct: 95 FFCLLQSHSPRCEAKQ 48
>gb|CW347611.1|CW347611 fsbb001f007j14k0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f007j14, DNA
sequence
Length = 609
Score = 25.4 bits (49), Expect(2) = 1.5
Identities = 7/22 (31%), Positives = 13/22 (59%)
Frame = +1 / -3
Query: 73 FPLIHMQSSWLWFSSTLQWR*H 138
F +H + W+W + + WR*+
Sbjct: 520 FIYMHQKLMWMWKTRYIMWR*Y 455
Score = 24.0 bits (46), Expect(2) = 1.5
Identities = 7/16 (43%), Positives = 10/16 (62%)
Frame = +2 / -3
Query: 167 YFCYCEDHSERC*GKE 214
+FC + HS RC K+
Sbjct: 358 FFCLLQSHSPRCEAKQ 311
>gb|CW240825.1|CW240825 104_699_11218426_148_37537_035 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11218426, DNA
sequence
Length = 668
Score = 25.4 bits (49), Expect(2) = 1.6
Identities = 7/22 (31%), Positives = 13/22 (59%)
Frame = +1 / -3
Query: 73 FPLIHMQSSWLWFSSTLQWR*H 138
F +H + W+W + + WR*+
Sbjct: 549 FIYMHQKLMWMWKTRYIMWR*Y 484
Score = 24.0 bits (46), Expect(2) = 1.6
Identities = 7/16 (43%), Positives = 10/16 (62%)
Frame = +2 / -3
Query: 167 YFCYCEDHSERC*GKE 214
+FC + HS RC K+
Sbjct: 387 FFCLLQSHSPRCEAKQ 340
>gb|CW240824.1|CW240824 104_699_11218426_116_37550_035 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11218426, DNA
sequence
Length = 669
Score = 25.4 bits (49), Expect(2) = 1.6
Identities = 7/22 (31%), Positives = 13/22 (59%)
Frame = +1 / +3
Query: 73 FPLIHMQSSWLWFSSTLQWR*H 138
F +H + W+W + + WR*+
Sbjct: 435 FIYMHQKLMWMWKTRYIMWR*Y 500
Score = 24.0 bits (46), Expect(2) = 1.6
Identities = 7/16 (43%), Positives = 10/16 (62%)
Frame = +2 / +3
Query: 167 YFCYCEDHSERC*GKE 214
+FC + HS RC K+
Sbjct: 597 FFCLLQSHSPRCEAKQ 644
>gb|CW450130.1|CW450130 fsbb001f188j18k0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f188j18, DNA
sequence
Length = 662
Score = 31.8 bits (63), Expect = 3.5
Identities = 15/39 (38%), Positives = 18/39 (46%)
Frame = -2 / +3
Query: 203 SIFLNGPHSNRNNLGKYTAFIWCYLH*RVLENHNHELCI 87
S+ L GP N N + AFI C L H HE C+
Sbjct: 150 SLPLQGPFFNVNTRRQQQAFIHCVLLPHSWHEHEHEHCV 266
>gb|CW324018.1|CW324018 104_818_11476935_116_35904_058 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11476935, DNA
sequence
Length = 679
Score = 24.9 bits (48), Expect(2) = 5.4
Identities = 7/10 (70%), Positives = 7/10 (70%)
Frame = +2 / -3
Query: 173 CYCEDHSERC 202
CYC HSE C
Sbjct: 524 CYCHHHSEYC 495
Score = 22.6 bits (43), Expect(2) = 5.4
Identities = 8/17 (47%), Positives = 13/17 (76%)
Frame = +3 / -2
Query: 126 VEITPNKGSILSKIISV 176
+EITPN+ S+ + IS+
Sbjct: 618 IEITPNRQSLPRRFISL 568
>gb|CW456179.1|CW456179 fsbb001f201g16f0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f201g16, DNA
sequence
Length = 759
Score = 24.9 bits (48), Expect(2) = 6.0
Identities = 7/10 (70%), Positives = 7/10 (70%)
Frame = +2 / -3
Query: 173 CYCEDHSERC 202
CYC HSE C
Sbjct: 520 CYCHHHSEYC 491
Score = 22.6 bits (43), Expect(2) = 6.0
Identities = 8/17 (47%), Positives = 13/17 (76%)
Frame = +3 / -2
Query: 126 VEITPNKGSILSKIISV 176
+EITPN+ S+ + IS+
Sbjct: 614 IEITPNRQSLPRRFISL 564
>gb|CF758854.1|CF758854 DSAF1_37_D05.g1_A011 Drought-stressed after flowering Sorghum
bicolor cDNA clone DSAF1_37_D05_A011 3', mRNA sequence
Length = 522
Score = 30.9 bits (61), Expect = 6.6
Identities = 10/28 (35%), Positives = 14/28 (50%)
Frame = +1 / -3
Query: 25 LVDHLSNHHSNPIFHIFPLIHMQSSWLW 108
L HL HH +P+F +F + W W
Sbjct: 280 LTAHLDYHHRHPLFVLFNYSPLTHRWTW 197
>gb|BG273626.1|BG273626 OV2_28_A03.b1_A002 Ovary 2 (OV2) Sorghum bicolor cDNA, mRNA
sequence
Length = 248
Score = 25.4 bits (49), Expect(2) = 8.6
Identities = 11/24 (45%), Positives = 13/24 (54%)
Frame = +1 / +3
Query: 67 HIFPLIHMQSSWLWFSSTLQWR*H 138
H+ PL H Q + SS LQW H
Sbjct: 12 HLTPLHHQQLTTPHCSSVLQWLLH 83
Score = 19.9 bits (37), Expect(2) = 8.6
Identities = 6/7 (85%), Positives = 6/7 (85%)
Frame = +3 / +1
Query: 282 DGHHDGS 302
DGHH GS
Sbjct: 214 DGHHGGS 234
>gb|BZ352165.1|BZ352165 hm86e05.g1 WGS-SbicolorF (JM107 adapted methyl filtered) Sorghum
bicolor genomic clone hm86e05 5', DNA sequence
Length = 550
Score = 30.4 bits (60), Expect = 9.1
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = +3 / +1
Query: 159 SKIISVTVRTIQKDAEEKKASFNPRPYFRLXINWLYDL 272
S II T+R E KA++NPR FR+ +N+ + +
Sbjct: 25 SSIIKDTIRPQGFLLEISKATYNPRNCFRIHVNFDFSI 138
>gb|CW246474.1|CW246474 104_707_11221355_116_35082_042 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11221355, DNA
sequence
Length = 730
Score = 30.4 bits (60), Expect = 9.1
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = +3 / -1
Query: 159 SKIISVTVRTIQKDAEEKKASFNPRPYFRLXINWLYDL 272
S II T+R E KA++NPR FR+ +N+ + +
Sbjct: 118 SSIIKDTIRPQGFLLEISKATYNPRNCFRIHVNFDFSI 5
>gb|CW397356.1|CW397356 fsbb001f086d19k0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f086d19, DNA
sequence
Length = 742
Score = 30.4 bits (60), Expect = 9.1
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = +3 / -2
Query: 159 SKIISVTVRTIQKDAEEKKASFNPRPYFRLXINWLYDL 272
S II T+R E KA++NPR FR+ +N+ + +
Sbjct: 315 SSIIKDTIRPQGFLLEISKATYNPRNCFRIHVNFDFSI 202
Database: Sorghum_nucl_with_EST.fasta
Posted date: May 2, 2006 3:37 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 315,158,633
Number of Sequences: 832831
Number of extensions: 3390410
Number of successful extensions: 243784
Number of sequences better than 10.0: 22
length of database: 163,786,556
effective HSP length: 51
effective length of database: 121,312,175
effective search space used: 6429545275
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)