TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF13b04.yg.2.1
(370 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF657000.1|BF657000 OV2_19_G06.b1_A002 Ovary 2 (OV2) Sor... 180 7e-045
gb|CL170431.1|CL170431 104_371_10813804_148_31790_124 Sorgh... 144 4e-034
gb|CD219775.1|CD219775 CCC1_58_A07.g1_A007 Callus culture/c... 144 8e-034
gb|CW243716.1|CW243716 104_704_11220201_116_37577_041 Sorgh... 135 3e-031
gb|CW256112.1|CW256112 104_721_11226607_148_35144_032 Sorgh... 110 1e-023
gb|BM325000.1|BM325000 PIC1_38_E07.b1_A002 Pathogen-infecte... 102 2e-021
gb|BG355549.1|BG355549 EM1_17_G07.b1_A002 Embryo 1 (EM1) So... 102 3e-021
gb|BI643696.1|BI643696 EM1_19_F12.b1_A002 Embryo 1 (EM1) So... 102 3e-021
gb|CL170430.1|CL170430 104_371_10813804_116_31791_124 Sorgh... 101 4e-021
>gb|BF657000.1|BF657000 OV2_19_G06.b1_A002 Ovary 2 (OV2) Sorghum bicolor cDNA, mRNA
sequence
Length = 455
Score = 180 bits (388), Expect = 7e-045
Identities = 75/111 (67%), Positives = 84/111 (75%)
Frame = +3 / +2
Query: 18 YIFLYGKMYLALSGVGESIQNRADIXXXXXXXXXXXTQFLFQIGVFTAVPMILGFILESG 197
YIFLYG++YLALSG+ SI +A QFL QIG+FTAVPMI+GFILE G
Sbjct: 56 YIFLYGRVYLALSGLDYSISRQARFLGNTALDAALNAQFLVQIGIFTAVPMIMGFILELG 235
Query: 198 VLTAFVQFITMQFQMCSVFFTFSLGTRTHYFGRAILHGGAKYRATGRGFVV 350
++ A FITMQ Q CSVFFTFSLGTRTHYFGR ILHGGAKY+ATGRGFVV
Sbjct: 236 LMKAIFSFITMQLQFCSVFFTFSLGTRTHYFGRTILHGGAKYKATGRGFVV 388
Score = 137 bits (294), Expect = 6e-032
Identities = 64/111 (57%), Positives = 74/111 (66%)
Frame = -1 / -3
Query: 349 TTKPLPVALYFAPPCNIARPK*WVLVPSEKVKNTEHIWNCIVIN*TKAVKTPDSRMKPRI 170
TTKPLPVALYFAPP I PK* VLVPSEKVKNTE W+CIVI A +P S+MKP I
Sbjct: 387 TTKPLPVALYFAPP*RIVLPK*CVLVPSEKVKNTEQNWSCIVIKLKIAFISPSSKMKPII 208
Query: 169 MGTAVNTPIWKRN*VFRAAFKAAFCSISALF*IDSPTPDKARYIFP*RNMY 17
+GTAV PIW +N* F+AA A F A I+ P+ ARY P + +Y
Sbjct: 207 IGTAVKIPIWTKN*AFKAASSAVFPKNLAWRDIE*SRPESARYTLPYKKIY 55
>gb|CL170431.1|CL170431 104_371_10813804_148_31790_124 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10813804, DNA
sequence
Length = 656
Score = 144 bits (310), Expect = 4e-034
Identities = 60/111 (54%), Positives = 75/111 (67%)
Frame = +3 / -2
Query: 18 YIFLYGKMYLALSGVGESIQNRADIXXXXXXXXXXXTQFLFQIGVFTAVPMILGFILESG 197
Y F++G+ YLALSG+ + I QF+ Q+G+FTA+PMI+ LE G
Sbjct: 481 YAFVWGRFYLALSGLEDYISKNTSSSNNAALGAVLNQQFVIQLGLFTALPMIIENSLEHG 302
Query: 198 VLTAFVQFITMQFQMCSVFFTFSLGTRTHYFGRAILHGGAKYRATGRGFVV 350
LTA F+ MQ Q SVF+TFS+GT+THY+GR ILHGGAKYRATGRGFVV
Sbjct: 301 FLTAAWDFMKMQLQFASVFYTFSMGTKTHYYGRTILHGGAKYRATGRGFVV 149
>gb|CD219775.1|CD219775 CCC1_58_A07.g1_A007 Callus culture/cell suspension Sorghum bicolor
cDNA clone CCC1_58_A07_A007 5', mRNA sequence
Length = 669
Score = 144 bits (308), Expect = 8e-034
Identities = 60/111 (54%), Positives = 75/111 (67%)
Frame = +3 / +3
Query: 18 YIFLYGKMYLALSGVGESIQNRADIXXXXXXXXXXXTQFLFQIGVFTAVPMILGFILESG 197
Y F++G+ YLALSG+ + I QF+ Q+G+FTA+PMI+ LE G
Sbjct: 78 YAFVWGRFYLALSGLEDYISMNTSSSNNAALGAVLNQQFVIQLGLFTALPMIIENSLEHG 257
Query: 198 VLTAFVQFITMQFQMCSVFFTFSLGTRTHYFGRAILHGGAKYRATGRGFVV 350
LTA F+ MQ Q SVF+TFS+GT+THY+GR ILHGGAKYRATGRGFVV
Sbjct: 258 FLTAAWDFMKMQLQFASVFYTFSMGTKTHYYGRTILHGGAKYRATGRGFVV 410
>gb|CW243716.1|CW243716 104_704_11220201_116_37577_041 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11220201, DNA
sequence
Length = 699
Score = 135 bits (289), Expect = 3e-031
Identities = 56/113 (49%), Positives = 71/113 (62%)
Frame = +3 / -1
Query: 12 GRYIFLYGKMYLALSGVGESIQNRADIXXXXXXXXXXXTQFLFQIGVFTAVPMILGFILE 191
G Y+FLYG++YL LSG+ ++ A +Q Q+G+ T +PM++ LE
Sbjct: 351 GVYVFLYGQLYLVLSGLQRALLLEAQTQNIKSLETALASQSFLQLGLLTGLPMVMELGLE 172
Query: 192 SGVLTAFVQFITMQFQMCSVFFTFSLGTRTHYFGRAILHGGAKYRATGRGFVV 350
G A FI MQ Q+ SVFFTFSLGT+ HY+GR ILHGGAKYR TGR FVV
Sbjct: 171 KGFRAALSDFILMQLQLASVFFTFSLGTKAHYYGRTILHGGAKYRPTGRKFVV 13
>gb|CW256112.1|CW256112 104_721_11226607_148_35144_032 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11226607, DNA
sequence
Length = 678
Score = 110 bits (234), Expect = 1e-023
Identities = 43/72 (59%), Positives = 54/72 (75%)
Frame = +3 / +1
Query: 135 LFQIGVFTAVPMILGFILESGVLTAFVQFITMQFQMCSVFFTFSLGTRTHYFGRAILHGG 314
+FQ+G+ +PM++ LE G A +F+ MQ Q+ VFFTF LGT+THY+GR ILHGG
Sbjct: 1 VFQLGMLLVLPMMMEVGLEKGFGRALAEFVIMQLQLAPVFFTFHLGTKTHYYGRTILHGG 180
Query: 315 AKYRATGRGFVV 350
AKYRATGRGFVV
Sbjct: 181 AKYRATGRGFVV 216
>gb|BM325000.1|BM325000 PIC1_38_E07.b1_A002 Pathogen-infected compatible 1 (PIC1) Sorghum
bicolor cDNA, mRNA sequence
Length = 558
Score = 102 bits (218), Expect = 2e-021
Identities = 42/75 (56%), Positives = 53/75 (70%)
Frame = +3 / +3
Query: 126 TQFLFQIGVFTAVPMILGFILESGVLTAFVQFITMQFQMCSVFFTFSLGTRTHYFGRAIL 305
+Q L Q+G A+PM++ LE G A I M Q+ +VFFTFSLGT+THY+GR +L
Sbjct: 72 SQSLVQLGFLMALPMMMEIGLERGFGQALSDLIMMNLQLATVFFTFSLGTKTHYYGRMLL 251
Query: 306 HGGAKYRATGRGFVV 350
HGGA+YR TGRGFVV
Sbjct: 252 HGGAQYRGTGRGFVV 296
>gb|BG355549.1|BG355549 EM1_17_G07.b1_A002 Embryo 1 (EM1) Sorghum bicolor cDNA, mRNA
sequence
Length = 502
Score = 102 bits (217), Expect = 3e-021
Identities = 40/44 (90%), Positives = 41/44 (93%)
Frame = +3 / +2
Query: 219 FITMQFQMCSVFFTFSLGTRTHYFGRAILHGGAKYRATGRGFVV 350
FITMQ Q CSVFFTFSLGTRTHYFGR ILHGGAKY+ATGRGFVV
Sbjct: 8 FITMQLQFCSVFFTFSLGTRTHYFGRTILHGGAKYKATGRGFVV 139
>gb|BI643696.1|BI643696 EM1_19_F12.b1_A002 Embryo 1 (EM1) Sorghum bicolor cDNA, mRNA
sequence
Length = 631
Score = 102 bits (217), Expect = 3e-021
Identities = 40/44 (90%), Positives = 41/44 (93%)
Frame = +3 / +2
Query: 219 FITMQFQMCSVFFTFSLGTRTHYFGRAILHGGAKYRATGRGFVV 350
FITMQ Q CSVFFTFSLGTRTHYFGR ILHGGAKY+ATGRGFVV
Sbjct: 8 FITMQLQFCSVFFTFSLGTRTHYFGRTILHGGAKYKATGRGFVV 139
>gb|CL170430.1|CL170430 104_371_10813804_116_31791_124 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10813804, DNA
sequence
Length = 746
Score = 101 bits (216), Expect = 4e-021
Identities = 42/92 (45%), Positives = 57/92 (61%)
Frame = +3 / +1
Query: 18 YIFLYGKMYLALSGVGESIQNRADIXXXXXXXXXXXTQFLFQIGVFTAVPMILGFILESG 197
Y F++G+ YLALSG+ + I QF+ Q+G+FTA+PMI+ LE G
Sbjct: 469 YAFVWGRFYLALSGLEDYISKNTSSSNNAALGAVLNQQFVIQLGLFTALPMIIENSLEHG 648
Query: 198 VLTAFVQFITMQFQMCSVFFTFSLGTRTHYFG 293
LTA F+ MQ Q SVF+TFS+GT+THY+G
Sbjct: 649 FLTAAWDFMKMQLQFASVFYTFSMGTKTHYYG 744
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 504,281,768
Number of Sequences: 832831
Number of extensions: 7224399
Number of successful extensions: 290535
Number of sequences better than 1.0e-020: 9
length of database: 163,786,556
effective HSP length: 51
effective length of database: 121,312,175
effective search space used: 8613164425
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)