TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAE37h07.yg.2.1
(642 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BI643696.1|BI643696 EM1_19_F12.b1_A002 Embryo 1 (EM1) So... 307 1e-082
gb|BG355549.1|BG355549 EM1_17_G07.b1_A002 Embryo 1 (EM1) So... 292 2e-078
gb|CF482724.1|CF482724 POL1_8_F04.g1_A002 Pollen Sorghum bi... 252 5e-066
gb|CD219775.1|CD219775 CCC1_58_A07.g1_A007 Callus culture/c... 189 4e-047
gb|CW256112.1|CW256112 104_721_11226607_148_35144_032 Sorgh... 178 2e-046
gb|CW414651.1|CW414651 fsbb001f114g15k0 Sorghum methylation... 179 4e-044
gb|CN147848.1|CN147848 WOUND1_52_D08.g1_A002 Wounded leaves... 157 7e-044
gb|BM325000.1|BM325000 PIC1_38_E07.b1_A002 Pathogen-infecte... 168 8e-041
gb|CW173113.1|CW173113 104_584_11156668_148_36547_006 Sorgh... 156 2e-039
gb|BF657000.1|BF657000 OV2_19_G06.b1_A002 Ovary 2 (OV2) Sor... 147 2e-034
gb|CL170431.1|CL170431 104_371_10813804_148_31790_124 Sorgh... 123 2e-027
gb|CD428230.1|CD428230 ETH1_28_F10.g1_A002 Ethylene-treated... 122 4e-027
gb|BZ338573.1|BZ338573 ia96c08.g1 WGS-SbicolorF (JM107 adap... 66 1e-022
>gb|BI643696.1|BI643696 EM1_19_F12.b1_A002 Embryo 1 (EM1) Sorghum bicolor cDNA, mRNA
sequence
Length = 631
Score = 307 bits (664), Expect = 1e-082
Identities = 135/176 (76%), Positives = 138/176 (78%)
Frame = +3 / -1
Query: 6 AILKHEKSXXXDCLKNTSSECLDMRLLLIPPRFPTXXTLYTNSTFIXXPXCPVIKVLNSL 185
AILK+EKS DCLKNTSSECLDM LLLIPPRFPT LYTNSTFI P CPVI+VLNSL
Sbjct: 535 AILKYEKSKAQDCLKNTSSECLDMLLLLIPPRFPTAIALYTNSTFIK*PVCPVIEVLNSL 356
Query: 186 LPLETRRVENVRREKPGHNEEPTTDSNQNK*R*TAXXXXICNXXXQEESNLKRFDKVTXR 365
LPLETRRVE VRREKPGHNEEPTTDSNQ K*R*TA I N +EESNLKRFDKVT R
Sbjct: 355 LPLETRRVEYVRREKPGHNEEPTTDSNQYK*R*TALRVSI*NVNDEEESNLKRFDKVTSR 176
Query: 366 IEPIIFSKXYVPXNKTSXSSTILCXXMKDXXTXXXXXCXKRKXXKHRTXLELHCNK 533
IEPIIFSK YVP NKTS SS ILC MKD T C KRK KHRT LELHCNK
Sbjct: 175 IEPIIFSKFYVPNNKTSSSSFILCTTMKDCSTKVMCPCSKRKGEKHRTELELHCNK 8
Score = 297 bits (642), Expect = 1e-079
Identities = 130/177 (73%), Positives = 134/177 (75%)
Frame = -1 / +1
Query: 531 YYNAAPXLFGVFXLFAXNXXXLXW*XNPSXXCKVSCYXXRFCCXAHXVC*KL*ALFXKSL 352
YYNAAP LFGVF LFA N L W* NPS CKV Y RFCC AH +C*KL*ALF KSL
Sbjct: 10 YYNAAPVLFGVFHLFAWNKDTLLW*NNPSWWCKV*SYWKRFCCSAHKIC*KL*ALFSKSL 189
Query: 351 CQSA*GCSPPDXXXCIXXXXRRFIFIYSDYYQ*LVPRYVLAFRALHFQPFWFRVAKDC*G 172
CQSA*GCSPP I RFIFIY+DYYQ*LVPRYVLAFRALH QPFWFRVAKDC*G
Sbjct: 190 CQSA*GCSPPHRLHFIWIHEGRFIFIYTDYYQ*LVPRYVLAFRALHIQPFWFRVAKDC*G 369
Query: 171 L**LDXXVXL*RWSWCKGXXXLGILVXXXXXAYPDIQRTYS*DNXQX*ISHVSVWXC 1
L *LD V L*RWSWCKG LGILV AYPDIQRTYS*DN + *ISH+SVW C
Sbjct: 370 LR*LDKLVTL*RWSWCKGR*QLGILVG*GAGAYPDIQRTYS*DNPEP*ISHISVWHC 540
Score = 284 bits (615), Expect = 8e-076
Identities = 135/178 (75%), Positives = 137/178 (76%)
Frame = +1 / -2
Query: 1 TXPY*NMRNLXLXIVSRIRPLNVWICACSSSHQDSQLXXXXXXXXXL*XXXXVQSSKSST 180
T PY*NMRNL L IVSRIRPLNVWIC+CSSSHQDSQL L* VQSSKSST
Sbjct: 540 TMPY*NMRNLRLRIVSRIRPLNVWICSCSSSHQDSQLLSPFTPTPPL*SNQFVQSSKSST 361
Query: 181 VFCHSKPEGLKM*GAKSQDITRNQLLIVIRINEDEPPXXXXYAXXXIRRRATSSALTK*L 360
VFCHSKPEGL M*GAKSQDITRNQLLIVI INEDEPP Y +RRRATSSALTK*L
Sbjct: 360 VFCHSKPEGLNM*GAKSQDITRNQLLIVISINEDEPPFVYPYEM*TMRRRATSSALTK*L 181
Query: 361 XE*SL*FSANXMCXTTKPLXVARYFAXX*RIXLPX*XXXVXSEKXKNTEQXWSCIVIK 534
E*SL*FSAN MC TTKPL VA YFA *RI LP * V SEK KNTEQ WSCIVIK
Sbjct: 180 RE*SL*FSANFMCRTTKPLPVALYFAPP*RIVLPK*CVLVPSEKVKNTEQNWSCIVIK 7
Score = 237 bits (513), Expect(2) = 3e-071
Identities = 99/131 (75%), Positives = 101/131 (77%)
Frame = -2 / +2
Query: 533 FITMQLQXCSVFXXFSLXTXXXXXGXXILHXXAKYRATXRGFVVXHIXFAENYRLYSXSH 354
FITMQLQ CSVF FSL T G ILH AKY+AT RGFVV HI FAENYRLYS SH
Sbjct: 8 FITMQLQFCSVFFTFSLGTRTHYFGRTILHGGAKYKATGRGFVVRHIKFAENYRLYSRSH 187
Query: 353 FVKALEVAXXXXXXIAYXXXXGGSSSFILITISSWFLVMSWLFAPYIFNPSGFEWQKTVE 174
FVKALEVA I+Y GGSSSFILITISSWFLVMSWLFAPYIFNPSGFEWQKTVE
Sbjct: 188 FVKALEVALLLIVYISYGYTKGGSSSFILITISSWFLVMSWLFAPYIFNPSGFEWQKTVE 367
Query: 173 DFDDWTXWXXY 141
DFDDWT W Y
Sbjct: 368 DFDDWTNWLLY 400
Score = 144 bits (309), Expect(2) = 1e-052
Identities = 65/78 (83%), Positives = 66/78 (84%)
Frame = -3 / +3
Query: 238 CPGFSRLTFSTLLVSSGKRLLRTLMTGXXGXFIKVELV*RVXXVGNLGGMRSRRISRHSE 59
CPGFSRLT+STLLVSSGKRLLRT MTG G FIKVELV*R VGNLGGMRSR ISRHSE
Sbjct: 303 CPGFSRLTYSTLLVSSGKRLLRTSMTGQTGYFIKVELV*RAIAVGNLGGMRSRSISRHSE 482
Query: 58 DVFLRQSXXXDFSCFSMA 5
DVFLRQS DFS FSMA
Sbjct: 483 DVFLRQS*ALDFSYFSMA 536
Score = 114 bits (244), Expect(2) = 5e-044
Identities = 61/94 (64%), Positives = 62/94 (65%)
Frame = +2 / -3
Query: 251 NY***SE*MKMNRLXXXXMQXXXSGGEQPQAL*QSDXXNRAYNFQQTLCAXQQNLXX*HD 430
NY***S *MKMNR M+ GGEQPQAL*QSD NRAYNFQQ LCA QQNL *
Sbjct: 290 NY***SV*MKMNRPSCIHMKCKR*GGEQPQAL*QSDFENRAYNFQQILCAEQQNLFQ*LY 111
Query: 431 TLXXXEGXXYXXNXXLXQAKXXKTPNXXGAAL** 532
TL EG Y N L QAK KTPN GAAL**
Sbjct: 110 TLHHHEGLFYQSNVSLFQAKR*KTPNRTGAAL** 9
Score = 84.5 bits (178), Expect(2) = 5e-044
Identities = 39/66 (59%), Positives = 39/66 (59%)
Frame = +2 / -3
Query: 5 CHTET*EIXXXXLSQEYVL*MSGYAPAPHPTKIPNXXXXXXXXXXYKXTXXXXXXXXXXX 184
CHTE *EI LSQEYVL*MSGYAPAPHPTKIPN YK T
Sbjct: 536 CHTEI*EI*GSGLSQEYVL*MSGYAPAPHPTKIPNCYRPLHQLHLYKVTSLSSHRSPQQS 357
Query: 185 FATRNQ 202
FATRNQ
Sbjct: 356 FATRNQ 339
Score = 83.5 bits (176), Expect(2) = 1e-052
Identities = 43/82 (52%), Positives = 44/82 (53%)
Frame = -3 / +3
Query: 532 LLQCSSXXVRCFXXFRLXQXXIXXVXXSFXXXQSIVLLXEVLLXGT*XLLKIIGSILXVT 353
LLQCSS VRCF FRL Q I V SF QSI LL EVLL GT* LLKIIGSIL VT
Sbjct: 9 LLQCSSSSVRCFSPFRLEQGHITLVEQSFMVVQSIKLLEEVLLFGT*NLLKIIGSILEVT 188
Query: 352 XXXXXXXXXXXXXXLHXXXXKA 287
H +A
Sbjct: 189 LSKRLRLLSSSSFTFHMDTRRA 254
Score = 52.4 bits (108), Expect(2) = 3e-071
Identities = 21/26 (80%), Positives = 22/26 (84%)
Frame = -2 / +2
Query: 83 QAHIQTFRGRILETIXXXRFLMFQYG 6
Q HIQTFRGRILETI RFL+FQYG
Sbjct: 458 QEHIQTFRGRILETILSLRFLIFQYG 535
>gb|BG355549.1|BG355549 EM1_17_G07.b1_A002 Embryo 1 (EM1) Sorghum bicolor cDNA, mRNA
sequence
Length = 502
Score = 292 bits (633), Expect = 2e-078
Identities = 128/165 (77%), Positives = 130/165 (78%)
Frame = +3 / -1
Query: 39 DCLKNTSSECLDMRLLLIPPRFPTXXTLYTNSTFIXXPXCPVIKVLNSLLPLETRRVENV 218
DCLKNTSSECLDM LLLIPPRFPT LYTNSTFI P CPVI+VLNSLLPLETRRVE V
Sbjct: 502 DCLKNTSSECLDMLLLLIPPRFPTAIALYTNSTFIK*PVCPVIEVLNSLLPLETRRVEYV 323
Query: 219 RREKPGHNEEPTTDSNQNK*R*TAXXXXICNXXXQEESNLKRFDKVTXRIEPIIFSKXYV 398
RREKPGHNEEPTTDSNQ K*R*TA I N +EESNLKRFDKVT RIEPIIFSK YV
Sbjct: 322 RREKPGHNEEPTTDSNQYK*R*TALRVSI*NVNDEEESNLKRFDKVTSRIEPIIFSKFYV 143
Query: 399 PXNKTSXSSTILCXXMKDXXTXXXXXCXKRKXXKHRTXLELHCNK 533
P NKTS SS ILC MKD T C KRK KHRT LELHCNK
Sbjct: 142 PNNKTSSSSFILCTTMKDCSTKVMCPCSKRKGEKHRTELELHCNK 8
Score = 276 bits (597), Expect = 2e-073
Identities = 122/164 (74%), Positives = 124/164 (75%)
Frame = -1 / +1
Query: 531 YYNAAPXLFGVFXLFAXNXXXLXW*XNPSXXCKVSCYXXRFCCXAHXVC*KL*ALFXKSL 352
YYNAAP LFGVF LFA N L W* NPS CKV Y RFCC AH +C*KL*ALF KSL
Sbjct: 10 YYNAAPVLFGVFHLFAWNKDTLLW*NNPSWWCKV*SYWKRFCCSAHKIC*KL*ALFSKSL 189
Query: 351 CQSA*GCSPPDXXXCIXXXXRRFIFIYSDYYQ*LVPRYVLAFRALHFQPFWFRVAKDC*G 172
CQSA*GCSPP I RFIFIY+DYYQ*LVPRYVLAFRALH QPFWFRVAKDC*G
Sbjct: 190 CQSA*GCSPPHRLHFIWIHEGRFIFIYTDYYQ*LVPRYVLAFRALHIQPFWFRVAKDC*G 369
Query: 171 L**LDXXVXL*RWSWCKGXXXLGILVXXXXXAYPDIQRTYS*DN 40
L *LD V L*RWSWCKG LGILV AYPDIQRTYS*DN
Sbjct: 370 LR*LDKLVTL*RWSWCKGR*QLGILVG*GAGAYPDIQRTYS*DN 501
Score = 263 bits (568), Expect = 2e-069
Identities = 125/165 (75%), Positives = 127/165 (76%)
Frame = +1 / -2
Query: 40 IVSRIRPLNVWICACSSSHQDSQLXXXXXXXXXL*XXXXVQSSKSSTVFCHSKPEGLKM* 219
IVSRIRPLNVWIC+CSSSHQDSQL L* VQSSKSSTVFCHSKPEGL M*
Sbjct: 501 IVSRIRPLNVWICSCSSSHQDSQLLSPFTPTPPL*SNQFVQSSKSSTVFCHSKPEGLNM* 322
Query: 220 GAKSQDITRNQLLIVIRINEDEPPXXXXYAXXXIRRRATSSALTK*LXE*SL*FSANXMC 399
GAKSQDITRNQLLIVI INEDEPP Y +RRRATSSALTK*L E*SL*FSAN MC
Sbjct: 321 GAKSQDITRNQLLIVISINEDEPPFVYPYEM*TMRRRATSSALTK*LRE*SL*FSANFMC 142
Query: 400 XTTKPLXVARYFAXX*RIXLPX*XXXVXSEKXKNTEQXWSCIVIK 534
TTKPL VA YFA *RI LP * V SEK KNTEQ WSCIVIK
Sbjct: 141 RTTKPLPVALYFAPP*RIVLPK*CVLVPSEKVKNTEQNWSCIVIK 7
Score = 237 bits (513), Expect(2) = 4e-066
Identities = 99/131 (75%), Positives = 101/131 (77%)
Frame = -2 / +2
Query: 533 FITMQLQXCSVFXXFSLXTXXXXXGXXILHXXAKYRATXRGFVVXHIXFAENYRLYSXSH 354
FITMQLQ CSVF FSL T G ILH AKY+AT RGFVV HI FAENYRLYS SH
Sbjct: 8 FITMQLQFCSVFFTFSLGTRTHYFGRTILHGGAKYKATGRGFVVRHIKFAENYRLYSRSH 187
Query: 353 FVKALEVAXXXXXXIAYXXXXGGSSSFILITISSWFLVMSWLFAPYIFNPSGFEWQKTVE 174
FVKALEVA I+Y GGSSSFILITISSWFLVMSWLFAPYIFNPSGFEWQKTVE
Sbjct: 188 FVKALEVALLLIVYISYGYTKGGSSSFILITISSWFLVMSWLFAPYIFNPSGFEWQKTVE 367
Query: 173 DFDDWTXWXXY 141
DFDDWT W Y
Sbjct: 368 DFDDWTNWLLY 400
Score = 129 bits (277), Expect(2) = 3e-048
Identities = 57/66 (86%), Positives = 58/66 (87%)
Frame = -3 / +3
Query: 238 CPGFSRLTFSTLLVSSGKRLLRTLMTGXXGXFIKVELV*RVXXVGNLGGMRSRRISRHSE 59
CPGFSRLT+STLLVSSGKRLLRT MTG G FIKVELV*R VGNLGGMRSR ISRHSE
Sbjct: 303 CPGFSRLTYSTLLVSSGKRLLRTSMTGQTGYFIKVELV*RAIAVGNLGGMRSRSISRHSE 482
Query: 58 DVFLRQ 41
DVFLRQ
Sbjct: 483 DVFLRQ 500
Score = 114 bits (244), Expect(2) = 6e-040
Identities = 61/94 (64%), Positives = 62/94 (65%)
Frame = +2 / -3
Query: 251 NY***SE*MKMNRLXXXXMQXXXSGGEQPQAL*QSDXXNRAYNFQQTLCAXQQNLXX*HD 430
NY***S *MKMNR M+ GGEQPQAL*QSD NRAYNFQQ LCA QQNL *
Sbjct: 290 NY***SV*MKMNRPSCIHMKCKR*GGEQPQAL*QSDFENRAYNFQQILCAEQQNLFQ*LY 111
Query: 431 TLXXXEGXXYXXNXXLXQAKXXKTPNXXGAAL** 532
TL EG Y N L QAK KTPN GAAL**
Sbjct: 110 TLHHHEGLFYQSNVSLFQAKR*KTPNRTGAAL** 9
Score = 83.5 bits (176), Expect(2) = 3e-048
Identities = 43/82 (52%), Positives = 44/82 (53%)
Frame = -3 / +3
Query: 532 LLQCSSXXVRCFXXFRLXQXXIXXVXXSFXXXQSIVLLXEVLLXGT*XLLKIIGSILXVT 353
LLQCSS VRCF FRL Q I V SF QSI LL EVLL GT* LLKIIGSIL VT
Sbjct: 9 LLQCSSSSVRCFSPFRLEQGHITLVEQSFMVVQSIKLLEEVLLFGT*NLLKIIGSILEVT 188
Query: 352 XXXXXXXXXXXXXXLHXXXXKA 287
H +A
Sbjct: 189 LSKRLRLLSSSSFTFHMDTRRA 254
Score = 70.7 bits (148), Expect(2) = 6e-040
Identities = 32/54 (59%), Positives = 32/54 (59%)
Frame = +2 / -3
Query: 41 LSQEYVL*MSGYAPAPHPTKIPNXXXXXXXXXXYKXTXXXXXXXXXXXFATRNQ 202
LSQEYVL*MSGYAPAPHPTKIPN YK T FATRNQ
Sbjct: 500 LSQEYVL*MSGYAPAPHPTKIPNCYRPLHQLHLYKVTSLSSHRSPQQSFATRNQ 339
Score = 35.0 bits (70), Expect(2) = 4e-066
Identities = 14/15 (93%), Positives = 14/15 (93%)
Frame = -2 / +2
Query: 83 QAHIQTFRGRILETI 39
Q HIQTFRGRILETI
Sbjct: 458 QEHIQTFRGRILETI 502
>gb|CF482724.1|CF482724 POL1_8_F04.g1_A002 Pollen Sorghum bicolor cDNA clone
POL1_8_F04_A002 5', mRNA sequence
Length = 773
Score = 252 bits (544), Expect = 5e-066
Identities = 112/140 (80%), Positives = 115/140 (82%)
Frame = +3 / -2
Query: 6 AILKHEKSXXXDCLKNTSSECLDMRLLLIPPRFPTXXTLYTNSTFIXXPXCPVIKVLNSL 185
AILK+EKS DCLKNTSSECLDM LLLIPPRFPT LYTNSTFI P CPVI+VLNSL
Sbjct: 460 AILKYEKSKAQDCLKNTSSECLDMLLLLIPPRFPTAIALYTNSTFIK*PVCPVIEVLNSL 281
Query: 186 LPLETRRVENVRREKPGHNEEPTTDSNQNK*R*TAXXXXICNXXXQEESNLKRFDKVTXR 365
LPLETRRVE VRREKPGHNEEPTTDSNQ K*R*TA I N +EESNLK FDKVT R
Sbjct: 280 LPLETRRVEYVRREKPGHNEEPTTDSNQYK*R*TALRVSI*NVNDEEESNLKCFDKVTSR 101
Query: 366 IEPIIFSKXYVPXNKTSXSS 425
IEPIIFSK YVP NKTS SS
Sbjct: 100 IEPIIFSKFYVPNNKTSSSS 41
Score = 242 bits (523), Expect = 4e-063
Identities = 105/141 (74%), Positives = 109/141 (77%)
Frame = -1 / +1
Query: 423 YXXRFCCXAHXVC*KL*ALFXKSLCQSA*GCSPPDXXXCIXXXXRRFIFIYSDYYQ*LVP 244
Y RFCC AH +C*KL*ALF KSLCQS *GCSPP I RFIFIY+DYYQ*LVP
Sbjct: 43 YWKRFCCSAHKIC*KL*ALFSKSLCQST*GCSPPHRLHFIWIHEGRFIFIYTDYYQ*LVP 222
Query: 243 RYVLAFRALHFQPFWFRVAKDC*GL**LDXXVXL*RWSWCKGXXXLGILVXXXXXAYPDI 64
RYVLAFRALH QPFWFRVAKDC*GL *LD V L*RWSWCKG LGILV AYPDI
Sbjct: 223 RYVLAFRALHIQPFWFRVAKDC*GLR*LDKLVTL*RWSWCKGR*QLGILVG*GAGAYPDI 402
Query: 63 QRTYS*DNXQX*ISHVSVWXC 1
QRTYS*DN + *ISH+SVW C
Sbjct: 403 QRTYS*DNPEP*ISHISVWHC 465
Score = 235 bits (507), Expect = 6e-061
Identities = 110/142 (77%), Positives = 112/142 (78%)
Frame = +1 / -3
Query: 1 TXPY*NMRNLXLXIVSRIRPLNVWICACSSSHQDSQLXXXXXXXXXL*XXXXVQSSKSST 180
T PY*NMRNL L IVSRIRPLNVWIC+CSSSHQDSQL L* VQSSKSST
Sbjct: 465 TMPY*NMRNLRLRIVSRIRPLNVWICSCSSSHQDSQLLSPFTPTPPL*SNQFVQSSKSST 286
Query: 181 VFCHSKPEGLKM*GAKSQDITRNQLLIVIRINEDEPPXXXXYAXXXIRRRATSSALTK*L 360
VFCHSKPEGL M*GAKSQDITRNQLLIVI INEDEPP Y +RRRATSSALTK*L
Sbjct: 285 VFCHSKPEGLNM*GAKSQDITRNQLLIVISINEDEPPFVYPYEM*TMRRRATSSALTK*L 106
Query: 361 XE*SL*FSANXMCXTTKPLXVA 426
E*SL*FSAN MC TTKPL VA
Sbjct: 105 RE*SL*FSANFMCRTTKPLPVA 40
Score = 189 bits (407), Expect(2) = 9e-057
Identities = 77/95 (81%), Positives = 78/95 (82%)
Frame = -2 / +2
Query: 425 ATXRGFVVXHIXFAENYRLYSXSHFVKALEVAXXXXXXIAYXXXXGGSSSFILITISSWF 246
AT RGFVV HI FAENYRLYS SHFVKALEVA I+Y GGSSSFILITISSWF
Sbjct: 41 ATGRGFVVRHIKFAENYRLYSRSHFVKALEVALLLIVYISYGYTKGGSSSFILITISSWF 220
Query: 245 LVMSWLFAPYIFNPSGFEWQKTVEDFDDWTXWXXY 141
LVMSWLFAPYIFNPSGFEWQKTVEDFDDWT W Y
Sbjct: 221 LVMSWLFAPYIFNPSGFEWQKTVEDFDDWTNWLLY 325
Score = 144 bits (309), Expect(2) = 5e-040
Identities = 65/78 (83%), Positives = 66/78 (84%)
Frame = -3 / +3
Query: 238 CPGFSRLTFSTLLVSSGKRLLRTLMTGXXGXFIKVELV*RVXXVGNLGGMRSRRISRHSE 59
CPGFSRLT+STLLVSSGKRLLRT MTG G FIKVELV*R VGNLGGMRSR ISRHSE
Sbjct: 228 CPGFSRLTYSTLLVSSGKRLLRTSMTGQTGYFIKVELV*RAIAVGNLGGMRSRSISRHSE 407
Query: 58 DVFLRQSXXXDFSCFSMA 5
DVFLRQS DFS FSMA
Sbjct: 408 DVFLRQS*ALDFSYFSMA 461
Score = 84.5 bits (178), Expect(2) = 2e-033
Identities = 39/66 (59%), Positives = 39/66 (59%)
Frame = +2 / -1
Query: 5 CHTET*EIXXXXLSQEYVL*MSGYAPAPHPTKIPNXXXXXXXXXXYKXTXXXXXXXXXXX 184
CHTE *EI LSQEYVL*MSGYAPAPHPTKIPN YK T
Sbjct: 461 CHTEI*EI*GSGLSQEYVL*MSGYAPAPHPTKIPNCYRPLHQLHLYKVTSLSSHRSPQQS 282
Query: 185 FATRNQ 202
FATRNQ
Sbjct: 281 FATRNQ 264
Score = 79.4 bits (167), Expect(2) = 2e-033
Identities = 39/55 (70%), Positives = 40/55 (72%)
Frame = +2 / -1
Query: 251 NY***SE*MKMNRLXXXXMQXXXSGGEQPQAL*QSDXXNRAYNFQQTLCAXQQNL 415
NY***S *MKMNR M+ GGEQPQ L*QSD NRAYNFQQ LCA QQNL
Sbjct: 215 NY***SV*MKMNRPSCIHMKCKR*GGEQPQVL*QSDFENRAYNFQQILCAEQQNL 51
Score = 52.4 bits (108), Expect(2) = 9e-057
Identities = 21/26 (80%), Positives = 22/26 (84%)
Frame = -2 / +2
Query: 83 QAHIQTFRGRILETIXXXRFLMFQYG 6
Q HIQTFRGRILETI RFL+FQYG
Sbjct: 383 QEHIQTFRGRILETILSLRFLIFQYG 460
Score = 41.4 bits (84), Expect(2) = 5e-040
Identities = 22/46 (47%), Positives = 23/46 (50%)
Frame = -3 / +3
Query: 424 LLXEVLLXGT*XLLKIIGSILXVTXXXXXXXXXXXXXXLHXXXXKA 287
LL EVLL GT* LLKIIGSIL VT H +A
Sbjct: 42 LLEEVLLFGT*NLLKIIGSILEVTLSKHLRLLSSSSFTFHMDTRRA 179
>gb|CD219775.1|CD219775 CCC1_58_A07.g1_A007 Callus culture/cell suspension Sorghum bicolor
cDNA clone CCC1_58_A07_A007 5', mRNA sequence
Length = 669
Score = 189 bits (407), Expect = 4e-047
Identities = 77/163 (47%), Positives = 99/163 (60%)
Frame = -2 / +3
Query: 638 AAXNAQFLVXXGIXXXVXMIMGXILXLXXMXXVXXFITMQLQXCSVFXXFSLXTXXXXXG 459
A N QF++ G+ + MI+ L + F+ MQLQ SVF FS+ T G
Sbjct: 174 AVLNQQFVIQLGLFTALPMIIENSLEHGFLTAAWDFMKMQLQFASVFYTFSMGTKTHYYG 353
Query: 458 XXILHXXAKYRATXRGFVVXHIXFAENYRLYSXSHFVKALEVAXXXXXXIAYXXXXGGSS 279
ILH AKYRAT RGFVV H FAENYRLY+ SHF+KA+E+ +Y G +
Sbjct: 354 RTILHGGAKYRATGRGFVVEHKKFAENYRLYARSHFIKAIELGVILTLYASYGSASGNTL 533
Query: 278 SFILITISSWFLVMSWLFAPYIFNPSGFEWQKTVEDFDDWTXW 150
+IL+T+SSWFLV SW+ AP+IFNPSGF+W K DF+D+ W
Sbjct: 534 VYILLTLSSWFLVSSWILAPFIFNPSGFDWLKNFNDFEDFLNW 662
>gb|CW256112.1|CW256112 104_721_11226607_148_35144_032 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11226607, DNA
sequence
Length = 678
Score = 178 bits (384), Expect(2) = 2e-046
Identities = 76/152 (50%), Positives = 91/152 (59%)
Frame = -2 / +1
Query: 605 GIXXXVXMIMGXILXLXXMXXVXXFITMQLQXCSVFXXFSLXTXXXXXGXXILHXXAKYR 426
G+ + M+M L + F+ MQLQ VF F L T G ILH AKYR
Sbjct: 13 GMLLVLPMMMEVGLEKGFGRALAEFVIMQLQLAPVFFTFHLGTKTHYYGRTILHGGAKYR 192
Query: 425 ATXRGFVVXHIXFAENYRLYSXSHFVKALEVAXXXXXXIAYXXXXGGSSSFILITISSWF 246
AT RGFVV H FAENYR+YS SHFVKALE+ +AY SS ++ +T+S WF
Sbjct: 193 ATGRGFVVRHAKFAENYRMYSRSHFVKALELLILLVVYLAYGSSYRSSSLYLYVTVSIWF 372
Query: 245 LVMSWLFAPYIFNPSGFEWQKTVEDFDDWTXW 150
LV WLFAP+IFNPS FEW KTV+D+ DW W
Sbjct: 373 LVFCWLFAPFIFNPSCFEWHKTVDDWIDWWKW 468
Score = 111 bits (236), Expect = 1e-023
Identities = 59/164 (35%), Positives = 83/164 (50%)
Frame = +3 / -1
Query: 93 PPRFPTXXTLYTNSTFIXXPXCPVIKVLNSLLPLETRRVENVRREKPGHNEEPTTDSNQN 272
PP PT +N+T I P PV V+NSL+PL+ VEN R ++P N+EP +S+
Sbjct: 525 PPSLPTLLRC*SNTTSIAHPLPPVNPVINSLVPLKAGWVENKRCKQPTENKEPYRNSDVQ 346
Query: 273 K*R*TAXXXXICNXXXQEESNLKRFDKVTXRIEPIIFSKXYVPXNKTSXSSTILCXXMKD 452
* + IC Q+ L+ F KVT + PII S+ +P NK S SS+ILC +K
Sbjct: 345 IQT*ASV*TPIC*VNNQKNKQLQCFHKVTPGVHPIILSELRMPHNKPSPSSSILCSTVKY 166
Query: 453 XXTXXXXXCXKRKXXKHRTXLELHCNKXXNXXHXXQXQNXXHYH 584
C K K ++R+ L+LH +K Q H+H
Sbjct: 165 CPAIVVSLCSKMKSEEYRS*LKLHNHKLCQCSAKSFLQANLHHH 34
Score = 28.6 bits (56), Expect(2) = 2e-046
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = -2 / +1
Query: 68 TFRGRILETIXXXRFLMFQYG 6
T R +LE I RFL++QYG
Sbjct: 556 TIRSLLLEFILSLRFLIYQYG 618
>gb|CW414651.1|CW414651 fsbb001f114g15k0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f114g15, DNA
sequence
Length = 636
Score = 179 bits (385), Expect = 4e-044
Identities = 70/128 (54%), Positives = 86/128 (67%)
Frame = -2 / +2
Query: 533 FITMQLQXCSVFXXFSLXTXXXXXGXXILHXXAKYRATXRGFVVXHIXFAENYRLYSXSH 354
F+ MQLQ SVF FSL T G +LH A+YRAT RGFVV H FAENYRLYS SH
Sbjct: 8 FVLMQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSH 187
Query: 353 FVKALEVAXXXXXXIAYXXXXGGSSSFILITISSWFLVMSWLFAPYIFNPSGFEWQKTVE 174
FVK +E+ + G+ ++I ITIS WF+V++WLFAP++FNPSGFEWQK V+
Sbjct: 188 FVKGIELMILLIVYEIFGQSYRGAIAYIFITISMWFMVVTWLFAPFLFNPSGFEWQKIVD 367
Query: 173 DFDDWTXW 150
D+ DW W
Sbjct: 368 DWTDWNKW 391
>gb|CN147848.1|CN147848 WOUND1_52_D08.g1_A002 Wounded leaves Sorghum bicolor cDNA clone
WOUND1_52_D08_A002 5', mRNA sequence
Length = 731
Score = 157 bits (337), Expect(2) = 7e-044
Identities = 58/92 (63%), Positives = 69/92 (75%)
Frame = -2 / +2
Query: 416 RGFVVXHIXFAENYRLYSXSHFVKALEVAXXXXXXIAYXXXXGGSSSFILITISSWFLVM 237
RGFVV HI FAENYR+Y+ SHFVK +EVA + Y GG+ +IL++ISSWF+ +
Sbjct: 2 RGFVVRHIKFAENYRIYARSHFVKGMEVALLLVIFLVYGFNNGGAVGYILLSISSWFMAL 181
Query: 236 SWLFAPYIFNPSGFEWQKTVEDFDDWTXWXXY 141
SWLFAPY+FNPSGFEWQK VEDF DWT W Y
Sbjct: 182 SWLFAPYLFNPSGFEWQKIVEDFRDWTNWLFY 277
Score = 132 bits (282), Expect = 6e-030
Identities = 70/139 (50%), Positives = 78/139 (56%)
Frame = +1 / -3
Query: 1 TXPY*NMRNLXLXIVSRIRPLNVWICACSSSHQDSQLXXXXXXXXXL*XXXXVQSSKSST 180
T PY M+NL L +VS I P + IC SSSHQ SQL * VQS KSST
Sbjct: 417 TTPYWKMKNLRLSMVSSILPRML*ICCSSSSHQASQLSSPLTPIPPR*NSQFVQSLKSST 238
Query: 181 VFCHSKPEGLKM*GAKSQDITRNQLLIVIRINEDEPPXXXXYAXXXIRRRATSSALTK*L 360
+FCHS P+GL *GAKSQD NQLLI +I PP Y + ATS TK L
Sbjct: 237 IFCHSNPDGLNK*GAKSQDNAINQLLIESKI*PTAPPLLNPYTRKITNKSATSIPFTKWL 58
Query: 361 XE*SL*FSANXMCXTTKPL 417
* *FSAN +C TTKPL
Sbjct: 57 RA*IR*FSANLICRTTKPL 1
Score = 41.4 bits (84), Expect(2) = 7e-044
Identities = 16/24 (66%), Positives = 18/24 (75%)
Frame = -2 / +2
Query: 77 HIQTFRGRILETIXXXRFLMFQYG 6
HI + RGRILETI RF +FQYG
Sbjct: 341 HIYSIRGRILETILSLRFFIFQYG 412
>gb|BM325000.1|BM325000 PIC1_38_E07.b1_A002 Pathogen-infected compatible 1 (PIC1) Sorghum
bicolor cDNA, mRNA sequence
Length = 558
Score = 168 bits (361), Expect = 8e-041
Identities = 73/159 (45%), Positives = 92/159 (57%)
Frame = -2 / +3
Query: 635 AXNAQFLVXXGIXXXVXMIMGXILXLXXMXXVXXFITMQLQXCSVFXXFSLXTXXXXXGX 456
A +Q LV G + M+M L + I M LQ +VF FSL T G
Sbjct: 63 ALASQSLVQLGFLMALPMMMEIGLERGFGQALSDLIMMNLQLATVFFTFSLGTKTHYYGR 242
Query: 455 XILHXXAKYRATXRGFVVXHIXFAENYRLYSXSHFVKALEVAXXXXXXIAYXXXXGGSSS 276
+LH A+YR T RGFVV H FAENYRLYS SHFVK +E+ + + +
Sbjct: 243 MLLHGGAQYRGTGRGFVVFHAKFAENYRLYSRSHFVKGIELMILLIVYQLFGQSYRSTIA 422
Query: 275 FILITISSWFLVMSWLFAPYIFNPSGFEWQKTVEDFDDW 159
+I ITIS WFLV++WLFAP++FNPSGFEW K V+D+ DW
Sbjct: 423 YIFITISMWFLVLTWLFAPFLFNPSGFEWAKIVDDWSDW 539
>gb|CW173113.1|CW173113 104_584_11156668_148_36547_006 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11156668, DNA
sequence
Length = 628
Score = 156 bits (335), Expect(2) = 2e-039
Identities = 61/104 (58%), Positives = 70/104 (67%)
Frame = -2 / +3
Query: 461 GXXILHXXAKYRATXRGFVVXHIXFAENYRLYSXSHFVKALEVAXXXXXXIAYXXXXGGS 282
G ILH AKYR T RGFVV H FAENYR+YS SHFVK LE+ + Y S
Sbjct: 9 GRTILHGGAKYRPTGRGFVVYHAKFAENYRMYSRSHFVKGLELLILLVVYLVYGRSYRSS 188
Query: 281 SSFILITISSWFLVMSWLFAPYIFNPSGFEWQKTVEDFDDWTXW 150
S ++ +T S WFLV SWLFAP+IFNPS FEWQKTV+D+ DW W
Sbjct: 189 SLYLFVTCSMWFLVASWLFAPFIFNPSCFEWQKTVDDWTDWRKW 320
Score = 27.2 bits (53), Expect(2) = 2e-039
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = -2 / +3
Query: 68 TFRGRILETIXXXRFLMFQYG 6
+ R +LE I RFL++QYG
Sbjct: 408 SIRALLLEIILSLRFLIYQYG 470
>gb|BF657000.1|BF657000 OV2_19_G06.b1_A002 Ovary 2 (OV2) Sorghum bicolor cDNA, mRNA
sequence
Length = 455
Score = 147 bits (315), Expect = 2e-034
Identities = 67/101 (66%), Positives = 69/101 (68%)
Frame = -2 / +2
Query: 638 AAXNAQFLVXXGIXXXVXMIMGXILXLXXMXXVXXFITMQLQXCSVFXXFSLXTXXXXXG 459
AA NAQFLV GI V MIMG IL L M + FITMQLQ CSVF FSL T G
Sbjct: 152 AALNAQFLVQIGIFTAVPMIMGFILELGLMKAIFSFITMQLQFCSVFFTFSLGTRTHYFG 331
Query: 458 XXILHXXAKYRATXRGFVVXHIXFAENYRLYSXSHFVKALE 336
ILH AKY+AT RGFVV HI FAENYRLYS SHFVKALE
Sbjct: 332 RTILHGGAKYKATGRGFVVRHIKFAENYRLYSRSHFVKALE 454
Score = 144 bits (310), Expect = 9e-034
Identities = 64/100 (64%), Positives = 65/100 (65%)
Frame = +3 / -2
Query: 336 LKRFDKVTXRIEPIIFSKXYVPXNKTSXSSTILCXXMKDXXTXXXXXCXKRKXXKHRTXL 515
LKRFDKVT RIEPIIFSK YVP NKTS SS ILC MKD T C KRK KHRT L
Sbjct: 454 LKRFDKVTSRIEPIIFSKFYVPNNKTSSSSFILCTTMKDCSTKVMCPCSKRKGEKHRTEL 275
Query: 516 ELHCNKXXNXXHXXQXQNXXHYHRXXXXNTXLXQKLSIXS 635
ELHCNK N H QN HYHR NT L Q+LSI S
Sbjct: 274 ELHCNKTKNSLHQP*LQNETHYHRYCCKNTNLDQELSIQS 155
Score = 139 bits (299), Expect = 3e-032
Identities = 63/102 (61%), Positives = 64/102 (62%)
Frame = -1 / +1
Query: 642 RXSXECSIFGXXXYFXXSXDDNGXHSXAGXDXXCXXLYYNAAPXLFGVFXLFAXNXXXLX 463
R ECSI G YF S DDNG H A D YYNAAP LFGVF LFA N L
Sbjct: 148 RCCFECSILGPDWYFYSSTDDNGFHFGARADEGYF*FYYNAAPVLFGVFHLFAWNKDTLL 327
Query: 462 W*XNPSXXCKVSCYXXRFCCXAHXVC*KL*ALFXKSLCQSA* 337
W* NPS CKV Y RFCC AH +C*KL*ALF KSLCQSA*
Sbjct: 328 W*NNPSWWCKV*SYWKRFCCSAHKIC*KL*ALFSKSLCQSA* 453
Score = 139 bits (298), Expect = 4e-032
Identities = 71/102 (69%), Positives = 72/102 (70%)
Frame = +1 / -3
Query: 337 SSALTK*LXE*SL*FSANXMCXTTKPLXVARYFAXX*RIXLPX*XXXVXSEKXKNTEQXW 516
SSALTK*L E*SL*FSAN MC TTKPL VA YFA *RI LP * V SEK KNTEQ W
Sbjct: 453 SSALTK*LRE*SL*FSANFMCRTTKPLPVALYFAPP*RIVLPK*CVLVPSEKVKNTEQNW 274
Query: 517 SCIVIKXXTXXIXXSXRMXPIIIXTXXKIPXXTKN*AFXAAS 642
SCIVIK I S +M PIII T KIP TKN*AF AAS
Sbjct: 273 SCIVIKLKIAFISPSSKMKPIIIGTAVKIPIWTKN*AFKAAS 148
Score = 115 bits (245), Expect = 8e-025
Identities = 61/99 (61%), Positives = 63/99 (63%)
Frame = +2 / -1
Query: 335 PQAL*QSDXXNRAYNFQQTLCAXQQNLXX*HDTLXXXEGXXYXXNXXLXQAKXXKTPNXX 514
PQAL*QSD NRAYNFQQ LCA QQNL * TL EG Y N L QAK KTPN
Sbjct: 455 PQAL*QSDFENRAYNFQQILCAEQQNLFQ*LYTLHHHEGLFYQSNVSLFQAKR*KTPNRT 276
Query: 515 GAAL**XXKXXXSXPAXE*XPLSSXLXXKYXSXPKIEHS 631
GAAL** K S A +* PLSS L KY S P+IEHS
Sbjct: 275 GAAL**N*K*PSSALAPK*NPLSSVLL*KYQSGPRIEHS 159
>gb|CL170431.1|CL170431 104_371_10813804_148_31790_124 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10813804, DNA
sequence
Length = 656
Score = 123 bits (264), Expect = 2e-027
Identities = 55/128 (42%), Positives = 72/128 (56%)
Frame = -2 / -2
Query: 638 AAXNAQFLVXXGIXXXVXMIMGXILXLXXMXXVXXFITMQLQXCSVFXXFSLXTXXXXXG 459
A N QF++ G+ + MI+ L + F+ MQLQ SVF FS+ T G
Sbjct: 385 AVLNQQFVIQLGLFTALPMIIENSLEHGFLTAAWDFMKMQLQFASVFYTFSMGTKTHYYG 206
Query: 458 XXILHXXAKYRATXRGFVVXHIXFAENYRLYSXSHFVKALEVAXXXXXXIAYXXXXGGSS 279
ILH AKYRAT RGFVV H FAENYRLY+ SHF+KA+E+ +Y G +
Sbjct: 205 RTILHGGAKYRATGRGFVVEHKKFAENYRLYARSHFIKAIELGVILTLYASYGSASGNTL 26
Query: 278 SFILITIS 255
+IL+T+S
Sbjct: 25 VYILLTLS 2
>gb|CD428230.1|CD428230 ETH1_28_F10.g1_A002 Ethylene-treated seedlings Sorghum bicolor cDNA
clone ETH1_28_F10_A002 5', mRNA sequence
Length = 533
Score = 122 bits (262), Expect = 4e-027
Identities = 47/90 (52%), Positives = 60/90 (66%)
Frame = -2 / +3
Query: 410 FVVXHIXFAENYRLYSXSHFVKALEVAXXXXXXIAYXXXXGGSSSFILITISSWFLVMSW 231
FVV H FAENYRLY+ SHF+KA+E+ A+ + +I++ ISSWFLV+SW
Sbjct: 9 FVVQHKRFAENYRLYARSHFIKAIELGIILTVYAAHSVIAKNTLVYIIMNISSWFLVVSW 188
Query: 230 LFAPYIFNPSGFEWQKTVEDFDDWTXWXXY 141
+ AP+ FNPSGF W KTV DFDD+ W Y
Sbjct: 189 IMAPFAFNPSGFGWLKTVYDFDDFMNWIWY 278
>gb|BZ338573.1|BZ338573 ia96c08.g1 WGS-SbicolorF (JM107 adapted methyl filtered) Sorghum
bicolor genomic clone ia96c08 5', DNA sequence
Length = 649
Score = 65.7 bits (137), Expect(2) = 1e-022
Identities = 27/34 (79%), Positives = 28/34 (82%)
Frame = +3 / -2
Query: 81 LLLIPPRFPTXXTLYTNSTFIXXPXCPVIKVLNS 182
LLLIPPRFPT LYTNSTFI P CPVI+VLNS
Sbjct: 327 LLLIPPRFPTAIALYTNSTFIK*PVCPVIEVLNS 226
Score = 61.6 bits (128), Expect(2) = 1e-022
Identities = 24/28 (85%), Positives = 25/28 (89%)
Frame = +1 / -3
Query: 1 TXPY*NMRNLXLXIVSRIRPLNVWICAC 84
T PY*NMRNL L IVSRIRPLNVWIC+C
Sbjct: 518 TMPY*NMRNLRLRIVSRIRPLNVWICSC 435
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 427,735,270
Number of Sequences: 832831
Number of extensions: 3597773
Number of successful extensions: 23774
Number of sequences better than 1.0e-020: 14
length of database: 163,786,556
effective HSP length: 53
effective length of database: 119,646,513
effective search space used: 19143442080
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)