TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 8635788.2.1
(753 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CW200341.1|CW200341 104_625_11184014_148_36859_049 Sorgh... 162 5e-039
gb|CN146142.1|CN146142 WOUND1_37_H10.g1_A002 Wounded leaves... 124 7e-036
gb|CN134822.1|CN134822 OX1_28_E09.g1_A002 Oxidatively-stres... 124 7e-036
gb|CN134747.1|CN134747 OX1_28_E09.b1_A002 Oxidatively-stres... 124 1e-032
gb|BI075862.1|BI075862 IP1_22_B10.b1_A002 Immature pannicle... 124 1e-029
gb|CN146081.1|CN146081 WOUND1_37_H10.b1_A002 Wounded leaves... 91 9e-023
>gb|CW200341.1|CW200341 104_625_11184014_148_36859_049 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11184014, DNA
sequence
Length = 396
Score = 162 bits (349), Expect = 5e-039
Identities = 71/86 (82%), Positives = 71/86 (82%)
Frame = +1 / +2
Query: 373 KTSLVQFHVVSAALPAAQLETVSNPLRTQAGDTGRGKYPLNLTADXXXXXXXXXXXXATL 552
KTSLVQFHVVSAAL AAQLETVSNPLRTQAGDTGRGKYPLNLTAD ATL
Sbjct: 2 KTSLVQFHVVSAALAAAQLETVSNPLRTQAGDTGRGKYPLNLTADGTNVNISTGVVNATL 181
Query: 553 DATPLYAGDRLVVYQVSKVLLPWALY 630
D TPLYAGDRLVVYQV KVLLPWALY
Sbjct: 182 DGTPLYAGDRLVVYQVDKVLLPWALY 259
Score = 137 bits (294), Expect = 2e-031
Identities = 61/87 (70%), Positives = 64/87 (73%)
Frame = -1 / -3
Query: 633 AVERPRQQHLADLVDDEPVPGVKRRGVERGVDDPRGDVDAGAVRGEVERVLAPAGVASLR 454
AVERP Q+HL DLVDDEPVPGV+RR VERGV P GDVD GAV GEVERVL+P GV LR
Sbjct: 262 AVERPWQEHLVDLVDDEPVPGVERRAVERGVHHPGGDVDVGAVGGEVERVLSPPGVPRLR 83
Query: 453 PQRVADXXXXXXXXXXGHDVELHQRRL 373
PQRVAD DVELHQRRL
Sbjct: 82 PQRVADRLQLRRRERRRDDVELHQRRL 2
Score = 103 bits (220), Expect = 3e-021
Identities = 42/45 (93%), Positives = 43/45 (95%)
Frame = -2 / -1
Query: 506 SAVRLSGYLPLPVSPACVRSGLLTVSSCAAGSAADTTWNCTSDVF 372
SAVRLSGY PLPVSPACVRSGLLTVSSCAA SAA+TTWNCTSDVF
Sbjct: 135 SAVRLSGYFPLPVSPACVRSGLLTVSSCAAASAAETTWNCTSDVF 1
>gb|CN146142.1|CN146142 WOUND1_37_H10.g1_A002 Wounded leaves Sorghum bicolor cDNA clone
WOUND1_37_H10_A002 5', mRNA sequence
Length = 750
Score = 124 bits (266), Expect(3) = 9e-035
Identities = 49/72 (68%), Positives = 62/72 (86%)
Frame = +1 / +3
Query: 292 GLTVFAPPDSAFSALPAGTLNSLSDQQKTSLVQFHVVSAALPAAQLETVSNPLRTQAGDT 471
G+TVFAP D+AF++LPAGTLNSLSDQ K +LVQ+HVVS A+P +Q +TVSNPLRTQAG
Sbjct: 339 GITVFAPTDNAFTSLPAGTLNSLSDQDKNALVQYHVVSTAIPMSQFDTVSNPLRTQAGSA 518
Query: 472 GRGKYPLNLTAD 507
G+YPLN+T++
Sbjct: 519 SPGEYPLNVTSE 554
Score = 110 bits (234), Expect(3) = 7e-036
Identities = 49/72 (68%), Positives = 51/72 (70%)
Frame = -2 / -3
Query: 506 SAVRLSGYLPLPVSPACVRSGLLTVSSCAAGSAADTTWNCTSDVFCWSERELSVPAGSAE 327
S V LSGY P PACVRSGLLTVS+C G A DTTW CTS WS+ ELSVPAGS
Sbjct: 553 SEVTLSGYSPGEALPACVRSGLLTVSNCDMGMAVDTTWYCTSAFLSWSDSELSVPAGSDV 374
Query: 326 KALSGGANTVSP 291
KALS GANTV P
Sbjct: 373 KALSVGANTVIP 338
Score = 41.4 bits (84), Expect(3) = 7e-036
Identities = 17/34 (50%), Positives = 22/34 (64%)
Frame = -2 / -3
Query: 272 LSWLVICSASRVDCSSWRNLVNCPALLRIAVMVL 171
L+WL IC A+ VD SS ++NCP L VM+L
Sbjct: 325 LTWLSICDATPVDSSSRMKVLNCPPLASTPVMLL 224
Score = 38.6 bits (78), Expect(3) = 7e-036
Identities = 18/26 (69%), Positives = 19/26 (73%)
Frame = -1 / -2
Query: 633 AVERPRQQHLADLVDDEPVPGVKRRG 556
A E RQQHL DLVDDE V GV+R G
Sbjct: 677 AEELLRQQHLVDLVDDEAVFGVERVG 600
Score = 36.8 bits (74), Expect(3) = 9e-035
Identities = 16/25 (64%), Positives = 18/25 (72%)
Frame = +1 / +3
Query: 556 ATPLYAGDRLVVYQVSKVLLPWALY 630
A LY+ D LVVYQV KVLLP L+
Sbjct: 600 ANSLYSEDSLVVYQVDKVLLPQKLF 674
Score = 24.9 bits (48), Expect(3) = 9e-035
Identities = 11/34 (32%), Positives = 12/34 (35%)
Frame = +3 / +2
Query: 171 QDHHGDPEQGRAVHQXXXXXXXXXXXXXXHQPAQ 272
+ HHG QGRAV QP Q
Sbjct: 224 EQHHGRAGQGRAVQHLHPAARVHGGGVADRQPGQ 325
>gb|CN134822.1|CN134822 OX1_28_E09.g1_A002 Oxidatively-stressed leaves and roots Sorghum
bicolor cDNA clone OX1_28_E09_A002 5', mRNA sequence
Length = 728
Score = 124 bits (266), Expect(3) = 1e-034
Identities = 49/72 (68%), Positives = 62/72 (86%)
Frame = +1 / +3
Query: 292 GLTVFAPPDSAFSALPAGTLNSLSDQQKTSLVQFHVVSAALPAAQLETVSNPLRTQAGDT 471
G+TVFAP D+AF++LPAGTLNSLSDQ K +LVQ+HVVS A+P +Q +TVSNPLRTQAG
Sbjct: 345 GITVFAPTDNAFTSLPAGTLNSLSDQDKNALVQYHVVSTAIPMSQFDTVSNPLRTQAGSA 524
Query: 472 GRGKYPLNLTAD 507
G+YPLN+T++
Sbjct: 525 SPGEYPLNVTSE 560
Score = 110 bits (234), Expect(3) = 7e-036
Identities = 49/72 (68%), Positives = 51/72 (70%)
Frame = -2 / -2
Query: 506 SAVRLSGYLPLPVSPACVRSGLLTVSSCAAGSAADTTWNCTSDVFCWSERELSVPAGSAE 327
S V LSGY P PACVRSGLLTVS+C G A DTTW CTS WS+ ELSVPAGS
Sbjct: 559 SEVTLSGYSPGEALPACVRSGLLTVSNCDMGMAVDTTWYCTSAFLSWSDSELSVPAGSDV 380
Query: 326 KALSGGANTVSP 291
KALS GANTV P
Sbjct: 379 KALSVGANTVIP 344
Score = 41.4 bits (84), Expect(3) = 7e-036
Identities = 17/34 (50%), Positives = 22/34 (64%)
Frame = -2 / -2
Query: 272 LSWLVICSASRVDCSSWRNLVNCPALLRIAVMVL 171
L+WL IC A+ VD SS ++NCP L VM+L
Sbjct: 331 LTWLSICDATPVDLSSRMKVLNCPPLASTPVMLL 230
Score = 38.6 bits (78), Expect(3) = 7e-036
Identities = 18/26 (69%), Positives = 19/26 (73%)
Frame = -1 / -1
Query: 633 AVERPRQQHLADLVDDEPVPGVKRRG 556
A E RQQHL DLVDDE V GV+R G
Sbjct: 683 AEELLRQQHLVDLVDDEAVFGVERVG 606
Score = 36.3 bits (73), Expect(3) = 1e-034
Identities = 16/25 (64%), Positives = 18/25 (72%)
Frame = +1 / +3
Query: 556 ATPLYAGDRLVVYQVSKVLLPWALY 630
A LY+ D LVVYQV KVLLP L+
Sbjct: 606 ADSLYSEDSLVVYQVDKVLLPQKLF 680
Score = 24.9 bits (48), Expect(3) = 1e-034
Identities = 11/34 (32%), Positives = 12/34 (35%)
Frame = +3 / +2
Query: 171 QDHHGDPEQGRAVHQXXXXXXXXXXXXXXHQPAQ 272
+ HHG QGRAV QP Q
Sbjct: 230 EQHHGRAGQGRAVQHLHPAAQVHGGGVADRQPGQ 331
>gb|CN134747.1|CN134747 OX1_28_E09.b1_A002 Oxidatively-stressed leaves and roots Sorghum
bicolor cDNA clone OX1_28_E09_A002 3', mRNA sequence
Length = 750
Score = 124 bits (266), Expect(2) = 1e-032
Identities = 49/72 (68%), Positives = 62/72 (86%)
Frame = +1 / +1
Query: 292 GLTVFAPPDSAFSALPAGTLNSLSDQQKTSLVQFHVVSAALPAAQLETVSNPLRTQAGDT 471
G+TVFAP D+AF++LPAGTLNSLSDQ K +LVQ+HVVS A+P +Q +TVSNPLRTQAG
Sbjct: 25 GITVFAPTDNAFTSLPAGTLNSLSDQDKNALVQYHVVSTAIPMSQFDTVSNPLRTQAGSA 204
Query: 472 GRGKYPLNLTAD 507
G+YPLN+T++
Sbjct: 205 SPGEYPLNVTSE 240
Score = 110 bits (234), Expect(2) = 6e-029
Identities = 49/72 (68%), Positives = 51/72 (70%)
Frame = -2 / -2
Query: 506 SAVRLSGYLPLPVSPACVRSGLLTVSSCAAGSAADTTWNCTSDVFCWSERELSVPAGSAE 327
S V LSGY P PACVRSGLLTVS+C G A DTTW CTS WS+ ELSVPAGS
Sbjct: 239 SEVTLSGYSPGEALPACVRSGLLTVSNCDMGMAVDTTWYCTSAFLSWSDSELSVPAGSDV 60
Query: 326 KALSGGANTVSP 291
KALS GANTV P
Sbjct: 59 KALSVGANTVIP 24
Score = 38.6 bits (78), Expect(2) = 6e-029
Identities = 18/26 (69%), Positives = 19/26 (73%)
Frame = -1 / -1
Query: 633 AVERPRQQHLADLVDDEPVPGVKRRG 556
A E RQQHL DLVDDE V GV+R G
Sbjct: 363 AEELLRQQHLVDLVDDEAVFGVERVG 286
Score = 36.8 bits (74), Expect(2) = 1e-032
Identities = 16/25 (64%), Positives = 18/25 (72%)
Frame = +1 / +1
Query: 556 ATPLYAGDRLVVYQVSKVLLPWALY 630
A LY+ D LVVYQV KVLLP L+
Sbjct: 286 ANSLYSEDSLVVYQVDKVLLPQKLF 360
>gb|BI075862.1|BI075862 IP1_22_B10.b1_A002 Immature pannicle 1 (IP1) Sorghum bicolor cDNA,
mRNA sequence
Length = 573
Score = 124 bits (266), Expect(2) = 3e-029
Identities = 49/72 (68%), Positives = 62/72 (86%)
Frame = +1 / +3
Query: 292 GLTVFAPPDSAFSALPAGTLNSLSDQQKTSLVQFHVVSAALPAAQLETVSNPLRTQAGDT 471
G+TVFAP D+AF++LPAGTLNSLSDQ K +LVQ+HVVS A+P +Q +TVSNPLRTQAG
Sbjct: 357 GITVFAPTDNAFTSLPAGTLNSLSDQDKNALVQYHVVSTAIPMSQFDTVSNPLRTQAGSA 536
Query: 472 GRGKYPLNLTAD 507
G+YPLN+T++
Sbjct: 537 SPGEYPLNVTSE 572
Score = 110 bits (234), Expect(2) = 1e-029
Identities = 49/72 (68%), Positives = 51/72 (70%)
Frame = -2 / -3
Query: 506 SAVRLSGYLPLPVSPACVRSGLLTVSSCAAGSAADTTWNCTSDVFCWSERELSVPAGSAE 327
S V LSGY P PACVRSGLLTVS+C G A DTTW CTS WS+ ELSVPAGS
Sbjct: 571 SEVTLSGYSPGEALPACVRSGLLTVSNCDMGMAVDTTWYCTSAFLSWSDSELSVPAGSDV 392
Query: 326 KALSGGANTVSP 291
KALS GANTV P
Sbjct: 391 KALSVGANTVIP 356
Score = 41.4 bits (84), Expect(2) = 1e-029
Identities = 17/34 (50%), Positives = 22/34 (64%)
Frame = -2 / -3
Query: 272 LSWLVICSASRVDCSSWRNLVNCPALLRIAVMVL 171
L+WL IC A+ VD SS ++NCP L VM+L
Sbjct: 343 LTWLSICDATPVDLSSRMKVLNCPPLASTPVMLL 242
Score = 24.9 bits (48), Expect(2) = 3e-029
Identities = 11/34 (32%), Positives = 12/34 (35%)
Frame = +3 / +2
Query: 171 QDHHGDPEQGRAVHQXXXXXXXXXXXXXXHQPAQ 272
+ HHG QGRAV QP Q
Sbjct: 242 EQHHGRAGQGRAVQHLHPAAQVHGGGVADRQPGQ 343
>gb|CN146081.1|CN146081 WOUND1_37_H10.b1_A002 Wounded leaves Sorghum bicolor cDNA clone
WOUND1_37_H10_A002 3', mRNA sequence
Length = 705
Score = 91.3 bits (193), Expect(2) = 9e-023
Identities = 36/54 (66%), Positives = 45/54 (83%)
Frame = +1 / +3
Query: 346 TLNSLSDQQKTSLVQFHVVSAALPAAQLETVSNPLRTQAGDTGRGKYPLNLTAD 507
TLNSLSDQ K +LVQ+HVVS A+P +Q +TVSNPLRTQAG G+YPLN+T++
Sbjct: 3 TLNSLSDQDKNALVQYHVVSTAIPMSQFDTVSNPLRTQAGSASPGEYPLNVTSE 164
Score = 36.8 bits (74), Expect(2) = 9e-023
Identities = 16/25 (64%), Positives = 18/25 (72%)
Frame = +1 / +3
Query: 556 ATPLYAGDRLVVYQVSKVLLPWALY 630
A LY+ D LVVYQV KVLLP L+
Sbjct: 210 ANSLYSEDSLVVYQVDKVLLPQKLF 284
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 377,180,619
Number of Sequences: 832831
Number of extensions: 3868658
Number of successful extensions: 45595
Number of sequences better than 1.0e-020: 6
length of database: 163,786,556
effective HSP length: 54
effective length of database: 118,813,682
effective search space used: 23287481672
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)