TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3748535.2.1
(619 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CX611601.1|CX611601 ANR1_25_D05.g1_A002 Anaerobic roots ... 224 8e-073
gb|CX617235.1|CX617235 GABR1_32_C02.g1_A002 GA- or brassino... 157 1e-053
gb|BI074628.1|BI074628 IP1_13_B09.b1_A002 Immature pannicle... 146 2e-049
gb|CF486612.1|CF486612 POL1_38_E10.g1_A002 Pollen Sorghum b... 128 5e-045
gb|BZ781676.1|BZ781676 ii30h03.b1 WGS-SbicolorF (DH5a methy... 79 4e-040
gb|BZ781677.1|BZ781677 ii30h03.b7 WGS-SbicolorF (DH5a methy... 79 4e-040
gb|CW479901.1|CW479901 fsbb001f238k14k0 Sorghum methylation... 79 1e-036
gb|CW257775.1|CW257775 104_723_11227518_116_35161_025 Sorgh... 79 4e-031
gb|CW438564.1|CW438564 fsbb001f156e04k0 Sorghum methylation... 67 4e-031
gb|CW479900.1|CW479900 fsbb001f238k14f0 Sorghum methylation... 79 9e-029
gb|CW246584.1|CW246584 104_707_11221402_148_35087_039 Sorgh... 74 5e-028
gb|CW472280.1|CW472280 fsbb001f227c06f0 Sorghum methylation... 74 3e-025
gb|CW321502.1|CW321502 104_814_11475589_116_35934_049 Sorgh... 68 5e-023
gb|CW144783.1|CW144783 104_537_11138124_116_34973_046 Sorgh... 53 7e-022
gb|CL178987.1|CL178987 104_387_10894976_148_31905_272 Sorgh... 51 1e-021
gb|CN146201.1|CN146201 WOUND1_38_C11.g1_A002 Wounded leaves... 57 9e-021
>gb|CX611601.1|CX611601 ANR1_25_D05.g1_A002 Anaerobic roots Sorghum bicolor cDNA clone
ANR1_25_D05_A002 5', mRNA sequence
Length = 804
Score = 224 bits (484), Expect(3) = 8e-073
Identities = 91/120 (75%), Positives = 102/120 (85%)
Frame = +2 / +1
Query: 260 GETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYHIS 439
GE+ RE+IKRH+HFLALQLGLK GMKVLDVGCGIGGPL EIARFSSTS+TGLNNN+Y I+
Sbjct: 394 GESLRESIKRHEHFLALQLGLKPGMKVLDVGCGIGGPLREIARFSSTSVTGLNNNEYQIT 573
Query: 440 RGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVLKP 619
RGKEL AG+S C F+K DFM MP DNTFDA YAI+ATCHAPD G Y+E+YRVLKP
Sbjct: 574 RGKELNRLAGVSGTCDFVKADFMKMPFDDNTFDAVYAIEATCHAPDPVGCYNEIYRVLKP 753
Score = 144 bits (309), Expect(2) = 4e-037
Identities = 68/120 (56%), Positives = 78/120 (65%)
Frame = -2 / -2
Query: 618 GFSTR*TSL*TPCASGXXXXXXXXXXASKVLSDIGMSMKSPFKKQHCSLKPAEEMSSLPL 439
GF+TR SL* P SG ASKVLS G+ MKS K L PA +SS PL
Sbjct: 752 GFNTRYISL*QPTGSGA*HVASIA*TASKVLSSNGIFMKSALTKSQVPLTPANRLSSFPL 573
Query: 438 EMW*SLLFSPVIDVELNLAISIKGPPIPHPTSKTFIPFLSPNCSAKKCWCRLMASREVSP 259
+W SLLF+PV +VELNLAIS+ GPPIP PTSKTFIP P+C A+KC RLM SR+ SP
Sbjct: 572 VIWYSLLFNPVTEVELNLAISLSGPPIPQPTSKTFIPGFKPSCRARKCS*RLMLSRKDSP 393
Score = 105 bits (223), Expect(2) = 5e-023
Identities = 55/114 (48%), Positives = 70/114 (61%)
Frame = -1 / -1
Query: 613 QHTVDLTIDALCIWCMTCSLYCIGRIKGVIRYWHVHEVPLQEAALLAQTC*RNELLAP*D 434
QHTV L I A I CMTC L CI IK VI WH+HEV L + +C ELL+P
Sbjct: 747 QHTVYLVIAANRIRCMTCCLNCINSIKSVIIKWHLHEVCLDKITGSTNSCQPIELLSPGY 568
Query: 433 VVVIVVQSSYRC*AESGDFY*RSPNSTSHIQNLHTLLEPQL*CQKMLVPLNGFT 272
+V IV+QSS *A+S + RS S +HIQNLH+ + +L +KML+ L+ FT
Sbjct: 567 LVFIVIQSSN*S*AKSRNLSQRSTYSAAHIQNLHSWFQAKLQGKKMLMTLDAFT 406
Score = 54.2 bits (112), Expect(3) = 8e-073
Identities = 21/33 (63%), Positives = 27/33 (81%)
Frame = +3 / +1
Query: 102 MAKSGALQLARSTGGSISKDQVKSAVDQYVKYH 200
M+K+GAL LA GG I+K++VKSAVD+Y KYH
Sbjct: 163 MSKAGALDLASGLGGKINKEEVKSAVDEYEKYH 261
Score = 35.4 bits (71), Expect(3) = 8e-073
Identities = 11/17 (64%), Positives = 17/17 (100%)
Frame = +1 / +1
Query: 208 HGGKQESRKSNYTDLIS 258
+GGK+E+RKSNYTD+++
Sbjct: 268 YGGKEEARKSNYTDMVN 318
Score = 33.6 bits (67), Expect(2) = 4e-037
Identities = 17/28 (60%), Positives = 18/28 (64%)
Frame = -1 / -2
Query: 196 YLTY*STADLTWSLLIDPPVLRASCSAP 113
Y +Y STADLT SLLI PP A AP
Sbjct: 257 YFSYSSTADLTSSLLIFPPRPDARSRAP 174
Score = 25.4 bits (49), Expect(2) = 5e-023
Identities = 12/22 (54%), Positives = 15/22 (68%)
Frame = -2 / -3
Query: 204 VRGI*HTDQRRI*LGPC*STLR 139
+ I HT + I*L PC*S+LR
Sbjct: 265 IHDISHTHRLPI*LPPC*SSLR 200
>gb|CX617235.1|CX617235 GABR1_32_C02.g1_A002 GA- or brassinolide-treated seedlings Sorghum
bicolor cDNA clone GABR1_32_C02_A002 5', mRNA sequence
Length = 777
Score = 157 bits (338), Expect(3) = 1e-053
Identities = 65/86 (75%), Positives = 74/86 (86%)
Frame = +2 / +1
Query: 260 GETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYHIS 439
GE+ RE+IKRH+HFLALQLGLK GMKVLDVGCGIGGPL EIARFSSTS+TGLNNN+Y I+
Sbjct: 520 GESLRESIKRHEHFLALQLGLKPGMKVLDVGCGIGGPLREIARFSSTSVTGLNNNEYQIT 699
Query: 440 RGKELISSAGLSEQCCFLKGDFMDMP 517
RGKEL AG+S C F++ DFM MP
Sbjct: 700 RGKELNRLAGISGTCDFVQADFMKMP 777
Score = 127 bits (273), Expect(2) = 2e-033
Identities = 53/86 (61%), Positives = 64/86 (74%)
Frame = -2 / -2
Query: 516 GMSMKSPFKKQHCSLKPAEEMSSLPLEMW*SLLFSPVIDVELNLAISIKGPPIPHPTSKT 337
G+ MKS + K L PA+ +SS PL +W SLLF+PV +VELNLAIS+ GPPIP PTSKT
Sbjct: 776 GIFMKSAWTKSQVPLIPAKRLSSFPLVIWYSLLFNPVTEVELNLAISLSGPPIPQPTSKT 597
Query: 336 FIPFLSPNCSAKKCWCRLMASREVSP 259
FIP P+C A+KC CRLM SR+ SP
Sbjct: 596 FIPGFKPSCRARKCSCRLMLSRKDSP 519
Score = 56.5 bits (117), Expect(3) = 1e-053
Identities = 23/36 (63%), Positives = 26/36 (72%)
Frame = +3 / +1
Query: 93 HQVMAKSGALQLARSTGGSISKDQVKSAVDQYVKYH 200
H M+KSGAL LA GG I K+ VKSAVD+Y KYH
Sbjct: 280 HYTMSKSGALDLASGLGGKIDKEGVKSAVDEYEKYH 387
Score = 37.7 bits (76), Expect(2) = 2e-033
Identities = 20/34 (58%), Positives = 21/34 (61%)
Frame = -1 / -2
Query: 196 YLTY*STADLTWSLLIDPPVLRASCSAPDLAIT* 95
Y +Y STAD T SL I PP A SAPDL I *
Sbjct: 383 YFSYSSTADFTPSLSIFPPRPEARSSAPDLDIV* 282
Score = 35.4 bits (71), Expect(3) = 1e-053
Identities = 11/17 (64%), Positives = 17/17 (100%)
Frame = +1 / +1
Query: 208 HGGKQESRKSNYTDLIS 258
+GGK+E+RKSNYTD+++
Sbjct: 394 YGGKEEARKSNYTDMVN 444
>gb|BI074628.1|BI074628 IP1_13_B09.b1_A002 Immature pannicle 1 (IP1) Sorghum bicolor cDNA,
mRNA sequence
Length = 515
Score = 146 bits (313), Expect(3) = 2e-049
Identities = 61/79 (77%), Positives = 69/79 (87%)
Frame = +2 / +1
Query: 260 GETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYHIS 439
GE+ RE+IKRH+HFLALQLGLK GMKVLDVGCGIGGPL EIARFSSTS+TGLNNN+Y I+
Sbjct: 274 GESLRESIKRHEHFLALQLGLKPGMKVLDVGCGIGGPLREIARFSSTSVTGLNNNEYQIT 453
Query: 440 RGKELISSAGLSEQCCFLK 496
RGKEL AG+S C F+K
Sbjct: 454 RGKELNRLAGVSGTCDFVK 510
Score = 115 bits (245), Expect(2) = 1e-028
Identities = 49/81 (60%), Positives = 57/81 (70%)
Frame = -2 / -1
Query: 501 SPFKKQHCSLKPAEEMSSLPLEMW*SLLFSPVIDVELNLAISIKGPPIPHPTSKTFIPFL 322
S K L PA +SS PL +W SLLF+PV +VELNLAIS+ GPPIP PTSKTFIP
Sbjct: 515 SALTKSQVPLTPANRLSSFPLVIWYSLLFNPVTEVELNLAISLSGPPIPQPTSKTFIPGF 336
Query: 321 SPNCSAKKCWCRLMASREVSP 259
P+C A+KC RLM SR+ SP
Sbjct: 335 KPSCRARKCS*RLMLSRKDSP 273
Score = 54.2 bits (112), Expect(3) = 2e-049
Identities = 21/33 (63%), Positives = 27/33 (81%)
Frame = +3 / +1
Query: 102 MAKSGALQLARSTGGSISKDQVKSAVDQYVKYH 200
M+K+GAL LA GG I+K++VKSAVD+Y KYH
Sbjct: 43 MSKAGALDLASGLGGKINKEEVKSAVDEYEKYH 141
Score = 35.4 bits (71), Expect(3) = 2e-049
Identities = 11/17 (64%), Positives = 17/17 (100%)
Frame = +1 / +1
Query: 208 HGGKQESRKSNYTDLIS 258
+GGK+E+RKSNYTD+++
Sbjct: 148 YGGKEEARKSNYTDMVN 198
Score = 33.6 bits (67), Expect(2) = 1e-028
Identities = 17/28 (60%), Positives = 18/28 (64%)
Frame = -1 / -1
Query: 196 YLTY*STADLTWSLLIDPPVLRASCSAP 113
Y +Y STADLT SLLI PP A AP
Sbjct: 137 YFSYSSTADLTSSLLIFPPRPDARSRAP 54
>gb|CF486612.1|CF486612 POL1_38_E10.g1_A002 Pollen Sorghum bicolor cDNA clone
POL1_38_E10_A002 5', mRNA sequence
Length = 738
Score = 128 bits (275), Expect(3) = 5e-045
Identities = 52/62 (83%), Positives = 58/62 (93%)
Frame = +2 / +1
Query: 260 GETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYHIS 439
GE+ RE+IKRH+HFLALQLGLK GMKVLDVGCGIGGPL EIARFSSTS+TGLNNN+Y I+
Sbjct: 553 GESLRESIKRHEHFLALQLGLKPGMKVLDVGCGIGGPLREIARFSSTSVTGLNNNEYQIT 732
Query: 440 RG 445
RG
Sbjct: 733 RG 738
Score = 107 bits (229), Expect(2) = 2e-027
Identities = 43/62 (69%), Positives = 50/62 (80%)
Frame = -2 / -2
Query: 444 PLEMW*SLLFSPVIDVELNLAISIKGPPIPHPTSKTFIPFLSPNCSAKKCWCRLMASREV 265
PL +W SLLF+PV +VELNLAIS+ GPPIP PTSKTFIP P+C A+KC CRLM SR+
Sbjct: 737 PLVIWYSLLFNPVTEVELNLAISLSGPPIPQPTSKTFIPGFKPSCRARKCSCRLMLSRKD 558
Query: 264 SP 259
SP
Sbjct: 557 SP 552
Score = 56.5 bits (117), Expect(3) = 5e-045
Identities = 23/36 (63%), Positives = 26/36 (72%)
Frame = +3 / +3
Query: 93 HQVMAKSGALQLARSTGGSISKDQVKSAVDQYVKYH 200
H M+KSGAL LA GG I K+ VKSAVD+Y KYH
Sbjct: 312 HYTMSKSGALDLASGLGGKIDKEGVKSAVDEYEKYH 419
Score = 37.7 bits (76), Expect(2) = 2e-027
Identities = 20/34 (58%), Positives = 21/34 (61%)
Frame = -1 / -3
Query: 196 YLTY*STADLTWSLLIDPPVLRASCSAPDLAIT* 95
Y +Y STAD T SL I PP A SAPDL I *
Sbjct: 415 YFSYSSTADFTPSLSIFPPRPEARSSAPDLDIV* 314
Score = 35.4 bits (71), Expect(3) = 5e-045
Identities = 11/17 (64%), Positives = 17/17 (100%)
Frame = +1 / +3
Query: 208 HGGKQESRKSNYTDLIS 258
+GGK+E+RKSNYTD+++
Sbjct: 426 YGGKEEARKSNYTDMVN 476
>gb|BZ781676.1|BZ781676 ii30h03.b1 WGS-SbicolorF (DH5a methyl filtered) Sorghum bicolor
genomic clone ii30h03, DNA sequence
Length = 707
Score = 79.4 bits (167), Expect(5) = 4e-040
Identities = 28/29 (96%), Positives = 28/29 (96%)
Frame = -1 / -1
Query: 577 IWCMTCSLYCIGRIKGVIRYWHVHEVPLQ 491
IWCMTCSLYCIGRIKGVI YWHVHEVPLQ
Sbjct: 536 IWCMTCSLYCIGRIKGVISYWHVHEVPLQ 450
Score = 70.3 bits (147), Expect(5) = 3e-036
Identities = 26/30 (86%), Positives = 30/30 (100%)
Frame = +2 / +3
Query: 491 LKGDFMDMPISDNTFDAAYAIQATCHAPDA 580
L+GDFM+MPI+DNTFDAAYAI+ATCHAPDA
Sbjct: 450 LQGDFMNMPIADNTFDAAYAIEATCHAPDA 539
Score = 68.9 bits (144), Expect(5) = 2e-032
Identities = 26/33 (78%), Positives = 29/33 (87%)
Frame = +1 / +2
Query: 481 MLLLEGGLHGHANI**HL*CGLCNTGYMSCTRC 579
+L++ GGLH HAN **HL*CGLCN GYMSCTRC
Sbjct: 440 LLVIAGGLHEHANS**HL*CGLCNRGYMSCTRC 538
Score = 60.6 bits (126), Expect(5) = 2e-031
Identities = 21/23 (91%), Positives = 23/23 (100%)
Frame = -3 / -3
Query: 578 HLVHDM*PVLHRPHQRCYQILAC 510
HLVHDM*P+LHRPHQRCYQ+LAC
Sbjct: 537 HLVHDM*PLLHRPHQRCYQLLAC 469
Score = 59.7 bits (124), Expect(5) = 4e-022
Identities = 27/38 (71%), Positives = 29/38 (76%)
Frame = +3 / +1
Query: 477 SNAAS*RGTSWTCQYLITPLMRPMQYRLHVMHQMHKAS 590
S+ + RGTS TCQ LITPLMRPMQ RLHVMHQM S
Sbjct: 436 SSPGNCRGTS*TCQ*LITPLMRPMQ*RLHVMHQMRCVS 549
Score = 47.8 bits (98), Expect(5) = 4e-040
Identities = 19/21 (90%), Positives = 20/21 (95%)
Frame = -2 / -2
Query: 402 DVELNLAISIKGPPIPHPTSK 340
D+ LNLAISIKGPPIPHPTSK
Sbjct: 79 DLCLNLAISIKGPPIPHPTSK 17
Score = 46.9 bits (96), Expect(5) = 3e-036
Identities = 16/20 (80%), Positives = 16/20 (80%)
Frame = +1 / +2
Query: 343 GCGMWNWGTFNRNRQIQLNI 402
GCGMWNWGT NRN QIQ I
Sbjct: 20 GCGMWNWGTLNRNCQIQAKI 79
Score = 45.5 bits (93), Expect(5) = 2e-032
Identities = 19/26 (73%), Positives = 20/26 (76%)
Frame = +2 / +3
Query: 341 LDVGCGIGGPLIEIARFSSTSITGLN 418
LDVGCGIGGPLIEIARF S L+
Sbjct: 18 LDVGCGIGGPLIEIARFRQRSALKLH 95
Score = 45.1 bits (92), Expect(5) = 2e-031
Identities = 15/22 (68%), Positives = 20/22 (90%)
Frame = -3 / -3
Query: 407 LSMLS*IWRFLLKVPQFHIPHP 342
+ + +*IW+FLL+VPQFHIPHP
Sbjct: 84 VQIFA*IWQFLLRVPQFHIPHP 19
Score = 40.9 bits (83), Expect(5) = 4e-040
Identities = 16/22 (72%), Positives = 18/22 (81%)
Frame = -3 / -3
Query: 458 K*APCPLRCGSHCCSVQLSMLS 393
K* PCPLR GS C S+QLS+LS
Sbjct: 240 K*KPCPLRSGSRCSSIQLSVLS 175
Score = 40.0 bits (81), Expect(5) = 2e-031
Identities = 17/22 (77%), Positives = 21/22 (95%)
Frame = -1 / -1
Query: 457 NELLAP*DVVVIVVQSSYRC*A 392
N+ LAP*D+VV+VVQSSY+C*A
Sbjct: 239 NKNLAP*DLVVVVVQSSYQC*A 174
Score = 40.0 bits (81), Expect(5) = 4e-022
Identities = 16/21 (76%), Positives = 17/21 (80%)
Frame = +3 / +1
Query: 336 RFWMWDVELGDL**KSPDSAQ 398
R WMWDVELGD **K PDS +
Sbjct: 13 RAWMWDVELGDP**KLPDSGK 75
Score = 38.2 bits (77), Expect(5) = 4e-040
Identities = 14/16 (87%), Positives = 16/16 (100%)
Frame = -2 / -3
Query: 495 FKKQHCSLKPAEEMSS 448
FKKQHCSLKPAE++SS
Sbjct: 360 FKKQHCSLKPAEKISS 313
Score = 38.2 bits (77), Expect(5) = 3e-036
Identities = 15/17 (88%), Positives = 16/17 (94%)
Frame = +2 / +2
Query: 446 KELISSAGLSEQCCFLK 496
+ELI SAGLSEQCCFLK
Sbjct: 311 QELIFSAGLSEQCCFLK 361
Score = 36.8 bits (74), Expect(5) = 3e-036
Identities = 16/22 (72%), Positives = 16/22 (72%)
Frame = +2 / +3
Query: 392 SSTSITGLNNNDYHISRGKELI 457
SS ITGLN NDY ISRGK I
Sbjct: 174 SSALITGLNYNDYQISRGKVFI 239
Score = 35.9 bits (72), Expect(5) = 2e-032
Identities = 14/22 (63%), Positives = 16/22 (72%)
Frame = +1 / +2
Query: 394 LNIDNWTEQQ*LPHLKGQGAHF 459
L+ DNW E Q LP LKGQG +F
Sbjct: 176 LSTDNWIELQRLPDLKGQGFYF 241
Score = 35.4 bits (71), Expect(5) = 2e-032
Identities = 15/17 (88%), Positives = 16/17 (94%)
Frame = +1 / +1
Query: 448 GAHFFSRFERAMLLLEG 498
GA+FFSRFE AMLLLEG
Sbjct: 313 GANFFSRFE*AMLLLEG 363
Score = 33.6 bits (67), Expect(5) = 3e-036
Identities = 13/13 (100%), Positives = 13/13 (100%)
Frame = +2 / +1
Query: 581 QGVYSEVYRVLKP 619
QGVYSEVYRVLKP
Sbjct: 652 QGVYSEVYRVLKP 690
Score = 32.7 bits (65), Expect(5) = 4e-040
Identities = 12/13 (92%), Positives = 13/13 (100%)
Frame = -1 / -3
Query: 619 RFQHTVDLTIDAL 581
RFQHTVDLT+DAL
Sbjct: 690 RFQHTVDLTVDAL 652
Score = 32.2 bits (64), Expect(5) = 2e-031
Identities = 12/18 (66%), Positives = 13/18 (72%)
Frame = -3 / -2
Query: 617 VSAHGRPHYRRLVHLVHD 564
VSAHGRPH RRL + D
Sbjct: 688 VSAHGRPHCRRLAAITRD 635
Score = 31.3 bits (62), Expect(5) = 2e-031
Identities = 14/17 (82%), Positives = 15/17 (88%)
Frame = -1 / -2
Query: 496 LQEAALLAQTC*RNELL 446
LQEAALL QTC*+N LL
Sbjct: 361 LQEAALLTQTC*KN*LL 311
Score = 27.6 bits (54), Expect(5) = 4e-022
Identities = 14/17 (82%), Positives = 14/17 (82%)
Frame = +3 / +3
Query: 447 RSSFLQQV*ASNAAS*R 497
RS F QQV* SNAAS*R
Sbjct: 312 RS*FFQQV*VSNAAS*R 362
Score = 27.2 bits (53), Expect(5) = 2e-032
Identities = 12/20 (60%), Positives = 13/20 (65%)
Frame = +1 / +3
Query: 559 YMSCTRCTRRL**GLPCAET 618
+ S RRL *GLPCAET
Sbjct: 630 FSSLVIAARRLQ*GLPCAET 689
Score = 25.8 bits (50), Expect(5) = 4e-022
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = +3 / +2
Query: 582 KASIVRSTVC*N 617
KAS VRSTVC*N
Sbjct: 653 KASTVRSTVC*N 688
Score = 23.5 bits (45), Expect(5) = 4e-022
Identities = 12/22 (54%), Positives = 12/22 (54%)
Frame = +3 / +1
Query: 393 AQHR*LDXXXXXXXSQGARSSF 458
AQH *LD SQGAR F
Sbjct: 175 AQH**LD*TTTTTRSQGARFLF 240
>gb|BZ781677.1|BZ781677 ii30h03.b7 WGS-SbicolorF (DH5a methyl filtered) Sorghum bicolor
genomic clone ii30h03, DNA sequence
Length = 674
Score = 79.4 bits (167), Expect(5) = 4e-040
Identities = 28/29 (96%), Positives = 28/29 (96%)
Frame = -1 / -2
Query: 577 IWCMTCSLYCIGRIKGVIRYWHVHEVPLQ 491
IWCMTCSLYCIGRIKGVI YWHVHEVPLQ
Sbjct: 520 IWCMTCSLYCIGRIKGVISYWHVHEVPLQ 434
Score = 70.3 bits (147), Expect(5) = 3e-036
Identities = 26/30 (86%), Positives = 30/30 (100%)
Frame = +2 / +2
Query: 491 LKGDFMDMPISDNTFDAAYAIQATCHAPDA 580
L+GDFM+MPI+DNTFDAAYAI+ATCHAPDA
Sbjct: 434 LQGDFMNMPIADNTFDAAYAIEATCHAPDA 523
Score = 68.9 bits (144), Expect(5) = 2e-032
Identities = 26/33 (78%), Positives = 29/33 (87%)
Frame = +1 / +1
Query: 481 MLLLEGGLHGHANI**HL*CGLCNTGYMSCTRC 579
+L++ GGLH HAN **HL*CGLCN GYMSCTRC
Sbjct: 424 LLVIAGGLHEHANS**HL*CGLCNRGYMSCTRC 522
Score = 60.6 bits (126), Expect(5) = 2e-031
Identities = 21/23 (91%), Positives = 23/23 (100%)
Frame = -3 / -1
Query: 578 HLVHDM*PVLHRPHQRCYQILAC 510
HLVHDM*P+LHRPHQRCYQ+LAC
Sbjct: 521 HLVHDM*PLLHRPHQRCYQLLAC 453
Score = 59.7 bits (124), Expect(5) = 1e-021
Identities = 27/38 (71%), Positives = 29/38 (76%)
Frame = +3 / +3
Query: 477 SNAAS*RGTSWTCQYLITPLMRPMQYRLHVMHQMHKAS 590
S+ + RGTS TCQ LITPLMRPMQ RLHVMHQM S
Sbjct: 420 SSPGNCRGTS*TCQ*LITPLMRPMQ*RLHVMHQMRCVS 533
Score = 47.8 bits (98), Expect(5) = 4e-040
Identities = 19/21 (90%), Positives = 20/21 (95%)
Frame = -2 / -3
Query: 402 DVELNLAISIKGPPIPHPTSK 340
D+ LNLAISIKGPPIPHPTSK
Sbjct: 63 DLCLNLAISIKGPPIPHPTSK 1
Score = 46.9 bits (96), Expect(5) = 3e-036
Identities = 16/20 (80%), Positives = 16/20 (80%)
Frame = +1 / +1
Query: 343 GCGMWNWGTFNRNRQIQLNI 402
GCGMWNWGT NRN QIQ I
Sbjct: 4 GCGMWNWGTLNRNCQIQAKI 63
Score = 45.5 bits (93), Expect(5) = 2e-032
Identities = 19/26 (73%), Positives = 20/26 (76%)
Frame = +2 / +2
Query: 341 LDVGCGIGGPLIEIARFSSTSITGLN 418
LDVGCGIGGPLIEIARF S L+
Sbjct: 2 LDVGCGIGGPLIEIARFRQRSALKLH 79
Score = 45.1 bits (92), Expect(5) = 2e-031
Identities = 15/22 (68%), Positives = 20/22 (90%)
Frame = -3 / -1
Query: 407 LSMLS*IWRFLLKVPQFHIPHP 342
+ + +*IW+FLL+VPQFHIPHP
Sbjct: 68 VQIFA*IWQFLLRVPQFHIPHP 3
Score = 40.9 bits (83), Expect(5) = 4e-040
Identities = 16/22 (72%), Positives = 18/22 (81%)
Frame = -3 / -1
Query: 458 K*APCPLRCGSHCCSVQLSMLS 393
K* PCPLR GS C S+QLS+LS
Sbjct: 224 K*KPCPLRSGSRCSSIQLSVLS 159
Score = 40.0 bits (81), Expect(5) = 2e-031
Identities = 17/22 (77%), Positives = 21/22 (95%)
Frame = -1 / -2
Query: 457 NELLAP*DVVVIVVQSSYRC*A 392
N+ LAP*D+VV+VVQSSY+C*A
Sbjct: 223 NKNLAP*DLVVVVVQSSYQC*A 158
Score = 38.6 bits (78), Expect(5) = 1e-021
Identities = 15/19 (78%), Positives = 16/19 (84%)
Frame = +3 / +3
Query: 342 WMWDVELGDL**KSPDSAQ 398
WMWDVELGD **K PDS +
Sbjct: 3 WMWDVELGDP**KLPDSGK 59
Score = 38.2 bits (77), Expect(5) = 4e-040
Identities = 14/16 (87%), Positives = 16/16 (100%)
Frame = -2 / -1
Query: 495 FKKQHCSLKPAEEMSS 448
FKKQHCSLKPAE++SS
Sbjct: 344 FKKQHCSLKPAEKISS 297
Score = 38.2 bits (77), Expect(5) = 3e-036
Identities = 15/17 (88%), Positives = 16/17 (94%)
Frame = +2 / +1
Query: 446 KELISSAGLSEQCCFLK 496
+ELI SAGLSEQCCFLK
Sbjct: 295 QELIFSAGLSEQCCFLK 345
Score = 36.8 bits (74), Expect(5) = 3e-036
Identities = 16/22 (72%), Positives = 16/22 (72%)
Frame = +2 / +2
Query: 392 SSTSITGLNNNDYHISRGKELI 457
SS ITGLN NDY ISRGK I
Sbjct: 158 SSALITGLNYNDYQISRGKVFI 223
Score = 35.9 bits (72), Expect(5) = 2e-032
Identities = 14/22 (63%), Positives = 16/22 (72%)
Frame = +1 / +1
Query: 394 LNIDNWTEQQ*LPHLKGQGAHF 459
L+ DNW E Q LP LKGQG +F
Sbjct: 160 LSTDNWIELQRLPDLKGQGFYF 225
Score = 35.4 bits (71), Expect(5) = 2e-032
Identities = 15/17 (88%), Positives = 16/17 (94%)
Frame = +1 / +3
Query: 448 GAHFFSRFERAMLLLEG 498
GA+FFSRFE AMLLLEG
Sbjct: 297 GANFFSRFE*AMLLLEG 347
Score = 33.6 bits (67), Expect(5) = 3e-036
Identities = 13/13 (100%), Positives = 13/13 (100%)
Frame = +2 / +3
Query: 581 QGVYSEVYRVLKP 619
QGVYSEVYRVLKP
Sbjct: 636 QGVYSEVYRVLKP 674
Score = 32.7 bits (65), Expect(5) = 4e-040
Identities = 12/13 (92%), Positives = 13/13 (100%)
Frame = -1 / -1
Query: 619 RFQHTVDLTIDAL 581
RFQHTVDLT+DAL
Sbjct: 674 RFQHTVDLTVDAL 636
Score = 32.2 bits (64), Expect(5) = 2e-031
Identities = 12/18 (66%), Positives = 13/18 (72%)
Frame = -3 / -3
Query: 617 VSAHGRPHYRRLVHLVHD 564
VSAHGRPH RRL + D
Sbjct: 672 VSAHGRPHCRRLAAITRD 619
Score = 31.3 bits (62), Expect(5) = 2e-031
Identities = 14/17 (82%), Positives = 15/17 (88%)
Frame = -1 / -3
Query: 496 LQEAALLAQTC*RNELL 446
LQEAALL QTC*+N LL
Sbjct: 345 LQEAALLTQTC*KN*LL 295
Score = 27.6 bits (54), Expect(5) = 1e-021
Identities = 14/17 (82%), Positives = 14/17 (82%)
Frame = +3 / +2
Query: 447 RSSFLQQV*ASNAAS*R 497
RS F QQV* SNAAS*R
Sbjct: 296 RS*FFQQV*VSNAAS*R 346
Score = 27.2 bits (53), Expect(5) = 2e-032
Identities = 12/20 (60%), Positives = 13/20 (65%)
Frame = +1 / +2
Query: 559 YMSCTRCTRRL**GLPCAET 618
+ S RRL *GLPCAET
Sbjct: 614 FSSLVIAARRLQ*GLPCAET 673
Score = 25.8 bits (50), Expect(5) = 1e-021
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = +3 / +1
Query: 582 KASIVRSTVC*N 617
KAS VRSTVC*N
Sbjct: 637 KASTVRSTVC*N 672
Score = 23.5 bits (45), Expect(5) = 1e-021
Identities = 12/22 (54%), Positives = 12/22 (54%)
Frame = +3 / +3
Query: 393 AQHR*LDXXXXXXXSQGARSSF 458
AQH *LD SQGAR F
Sbjct: 159 AQH**LD*TTTTTRSQGARFLF 224
>gb|CW479901.1|CW479901 fsbb001f238k14k0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f238k14, DNA
sequence
Length = 713
Score = 79.4 bits (167), Expect(4) = 1e-036
Identities = 28/29 (96%), Positives = 28/29 (96%)
Frame = -1 / -3
Query: 577 IWCMTCSLYCIGRIKGVIRYWHVHEVPLQ 491
IWCMTCSLYCIGRIKGVI YWHVHEVPLQ
Sbjct: 654 IWCMTCSLYCIGRIKGVISYWHVHEVPLQ 568
Score = 70.3 bits (147), Expect(4) = 3e-032
Identities = 26/30 (86%), Positives = 30/30 (100%)
Frame = +2 / +1
Query: 491 LKGDFMDMPISDNTFDAAYAIQATCHAPDA 580
L+GDFM+MPI+DNTFDAAYAI+ATCHAPDA
Sbjct: 568 LQGDFMNMPIADNTFDAAYAIEATCHAPDA 657
Score = 68.9 bits (144), Expect(4) = 2e-030
Identities = 26/33 (78%), Positives = 29/33 (87%)
Frame = +1 / +3
Query: 481 MLLLEGGLHGHANI**HL*CGLCNTGYMSCTRC 579
+L++ GGLH HAN **HL*CGLCN GYMSCTRC
Sbjct: 558 LLVIAGGLHEHANS**HL*CGLCNRGYMSCTRC 656
Score = 60.6 bits (126), Expect(4) = 2e-028
Identities = 21/23 (91%), Positives = 23/23 (100%)
Frame = -3 / -2
Query: 578 HLVHDM*PVLHRPHQRCYQILAC 510
HLVHDM*P+LHRPHQRCYQ+LAC
Sbjct: 655 HLVHDM*PLLHRPHQRCYQLLAC 587
Score = 51.5 bits (106), Expect(4) = 1e-036
Identities = 20/23 (86%), Positives = 22/23 (95%)
Frame = -2 / -1
Query: 402 DVELNLAISIKGPPIPHPTSKTF 334
D+ LNLAISIKGPPIPHPTSKT+
Sbjct: 197 DLCLNLAISIKGPPIPHPTSKTY 129
Score = 49.6 bits (102), Expect(4) = 2e-028
Identities = 16/25 (64%), Positives = 23/25 (92%)
Frame = -3 / -2
Query: 407 LSMLS*IWRFLLKVPQFHIPHPKPS 333
+ + +*IW+FLL+VPQFHIPHP+P+
Sbjct: 202 VQIFA*IWQFLLRVPQFHIPHPRPT 128
Score = 49.6 bits (102), Expect(4) = 3e-032
Identities = 17/22 (77%), Positives = 17/22 (77%)
Frame = +1 / +3
Query: 337 GFGCGMWNWGTFNRNRQIQLNI 402
G GCGMWNWGT NRN QIQ I
Sbjct: 132 GLGCGMWNWGTLNRNCQIQAKI 197
Score = 48.7 bits (100), Expect(4) = 2e-030
Identities = 24/37 (64%), Positives = 26/37 (70%)
Frame = +2 / +1
Query: 308 LQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLN 418
L L LK + VLDVGCGIGGPLIEIARF S L+
Sbjct: 103 LGL*LKLLL*VLDVGCGIGGPLIEIARFRQRSALKLH 213
Score = 40.9 bits (83), Expect(4) = 1e-036
Identities = 16/22 (72%), Positives = 18/22 (81%)
Frame = -3 / -2
Query: 458 K*APCPLRCGSHCCSVQLSMLS 393
K* PCPLR GS C S+QLS+LS
Sbjct: 358 K*KPCPLRSGSRCSSIQLSVLS 293
Score = 40.0 bits (81), Expect(4) = 2e-028
Identities = 17/22 (77%), Positives = 21/22 (95%)
Frame = -1 / -3
Query: 457 NELLAP*DVVVIVVQSSYRC*A 392
N+ LAP*D+VV+VVQSSY+C*A
Sbjct: 357 NKNLAP*DLVVVVVQSSYQC*A 292
Score = 38.2 bits (77), Expect(4) = 3e-032
Identities = 15/17 (88%), Positives = 16/17 (94%)
Frame = +2 / +3
Query: 446 KELISSAGLSEQCCFLK 496
+ELI SAGLSEQCCFLK
Sbjct: 429 QELIFSAGLSEQCCFLK 479
Score = 38.2 bits (77), Expect(4) = 1e-036
Identities = 14/16 (87%), Positives = 16/16 (100%)
Frame = -2 / -2
Query: 495 FKKQHCSLKPAEEMSS 448
FKKQHCSLKPAE++SS
Sbjct: 478 FKKQHCSLKPAEKISS 431
Score = 36.8 bits (74), Expect(4) = 3e-032
Identities = 16/22 (72%), Positives = 16/22 (72%)
Frame = +2 / +1
Query: 392 SSTSITGLNNNDYHISRGKELI 457
SS ITGLN NDY ISRGK I
Sbjct: 292 SSALITGLNYNDYQISRGKVFI 357
Score = 35.9 bits (72), Expect(4) = 2e-030
Identities = 14/22 (63%), Positives = 16/22 (72%)
Frame = +1 / +3
Query: 394 LNIDNWTEQQ*LPHLKGQGAHF 459
L+ DNW E Q LP LKGQG +F
Sbjct: 294 LSTDNWIELQRLPDLKGQGFYF 359
Score = 35.4 bits (71), Expect(4) = 2e-030
Identities = 15/17 (88%), Positives = 16/17 (94%)
Frame = +1 / +2
Query: 448 GAHFFSRFERAMLLLEG 498
GA+FFSRFE AMLLLEG
Sbjct: 431 GANFFSRFE*AMLLLEG 481
Score = 31.3 bits (62), Expect(4) = 2e-028
Identities = 14/17 (82%), Positives = 15/17 (88%)
Frame = -1 / -1
Query: 496 LQEAALLAQTC*RNELL 446
LQEAALL QTC*+N LL
Sbjct: 479 LQEAALLTQTC*KN*LL 429
>gb|CW257775.1|CW257775 104_723_11227518_116_35161_025 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11227518, DNA
sequence
Length = 724
Score = 79.4 bits (167), Expect(4) = 4e-031
Identities = 28/29 (96%), Positives = 28/29 (96%)
Frame = -1 / -2
Query: 577 IWCMTCSLYCIGRIKGVIRYWHVHEVPLQ 491
IWCMTCSLYCIGRIKGVI YWHVHEVPLQ
Sbjct: 387 IWCMTCSLYCIGRIKGVISYWHVHEVPLQ 301
Score = 70.3 bits (147), Expect(4) = 1e-027
Identities = 26/30 (86%), Positives = 30/30 (100%)
Frame = +2 / +1
Query: 491 LKGDFMDMPISDNTFDAAYAIQATCHAPDA 580
L+GDFM+MPI+DNTFDAAYAI+ATCHAPDA
Sbjct: 301 LQGDFMNMPIADNTFDAAYAIEATCHAPDA 390
Score = 68.9 bits (144), Expect(4) = 3e-024
Identities = 26/33 (78%), Positives = 29/33 (87%)
Frame = +1 / +3
Query: 481 MLLLEGGLHGHANI**HL*CGLCNTGYMSCTRC 579
+L++ GGLH HAN **HL*CGLCN GYMSCTRC
Sbjct: 291 LLVIAGGLHEHANS**HL*CGLCNRGYMSCTRC 389
Score = 60.6 bits (126), Expect(4) = 3e-023
Identities = 21/23 (91%), Positives = 23/23 (100%)
Frame = -3 / -1
Query: 578 HLVHDM*PVLHRPHQRCYQILAC 510
HLVHDM*P+LHRPHQRCYQ+LAC
Sbjct: 388 HLVHDM*PLLHRPHQRCYQLLAC 320
Score = 40.9 bits (83), Expect(4) = 4e-031
Identities = 16/22 (72%), Positives = 18/22 (81%)
Frame = -3 / -1
Query: 458 K*APCPLRCGSHCCSVQLSMLS 393
K* PCPLR GS C S+QLS+LS
Sbjct: 91 K*KPCPLRSGSRCSSIQLSVLS 26
Score = 40.0 bits (81), Expect(4) = 3e-023
Identities = 17/22 (77%), Positives = 21/22 (95%)
Frame = -1 / -2
Query: 457 NELLAP*DVVVIVVQSSYRC*A 392
N+ LAP*D+VV+VVQSSY+C*A
Sbjct: 90 NKNLAP*DLVVVVVQSSYQC*A 25
Score = 38.2 bits (77), Expect(4) = 4e-031
Identities = 14/16 (87%), Positives = 16/16 (100%)
Frame = -2 / -1
Query: 495 FKKQHCSLKPAEEMSS 448
FKKQHCSLKPAE++SS
Sbjct: 211 FKKQHCSLKPAEKISS 164
Score = 38.2 bits (77), Expect(4) = 1e-027
Identities = 15/17 (88%), Positives = 16/17 (94%)
Frame = +2 / +3
Query: 446 KELISSAGLSEQCCFLK 496
+ELI SAGLSEQCCFLK
Sbjct: 162 QELIFSAGLSEQCCFLK 212
Score = 36.8 bits (74), Expect(4) = 1e-027
Identities = 16/22 (72%), Positives = 16/22 (72%)
Frame = +2 / +1
Query: 392 SSTSITGLNNNDYHISRGKELI 457
SS ITGLN NDY ISRGK I
Sbjct: 25 SSALITGLNYNDYQISRGKVFI 90
Score = 35.9 bits (72), Expect(4) = 3e-024
Identities = 14/22 (63%), Positives = 16/22 (72%)
Frame = +1 / +3
Query: 394 LNIDNWTEQQ*LPHLKGQGAHF 459
L+ DNW E Q LP LKGQG +F
Sbjct: 27 LSTDNWIELQRLPDLKGQGFYF 92
Score = 35.4 bits (71), Expect(4) = 3e-024
Identities = 15/17 (88%), Positives = 16/17 (94%)
Frame = +1 / +2
Query: 448 GAHFFSRFERAMLLLEG 498
GA+FFSRFE AMLLLEG
Sbjct: 164 GANFFSRFE*AMLLLEG 214
Score = 33.6 bits (67), Expect(4) = 1e-027
Identities = 13/13 (100%), Positives = 13/13 (100%)
Frame = +2 / +2
Query: 581 QGVYSEVYRVLKP 619
QGVYSEVYRVLKP
Sbjct: 503 QGVYSEVYRVLKP 541
Score = 32.7 bits (65), Expect(4) = 4e-031
Identities = 12/13 (92%), Positives = 13/13 (100%)
Frame = -1 / -1
Query: 619 RFQHTVDLTIDAL 581
RFQHTVDLT+DAL
Sbjct: 541 RFQHTVDLTVDAL 503
Score = 32.2 bits (64), Expect(4) = 3e-023
Identities = 12/18 (66%), Positives = 13/18 (72%)
Frame = -3 / -3
Query: 617 VSAHGRPHYRRLVHLVHD 564
VSAHGRPH RRL + D
Sbjct: 539 VSAHGRPHCRRLAAITRD 486
Score = 31.3 bits (62), Expect(4) = 3e-023
Identities = 14/17 (82%), Positives = 15/17 (88%)
Frame = -1 / -3
Query: 496 LQEAALLAQTC*RNELL 446
LQEAALL QTC*+N LL
Sbjct: 212 LQEAALLTQTC*KN*LL 162
Score = 27.2 bits (53), Expect(4) = 3e-024
Identities = 12/20 (60%), Positives = 13/20 (65%)
Frame = +1 / +1
Query: 559 YMSCTRCTRRL**GLPCAET 618
+ S RRL *GLPCAET
Sbjct: 481 FSSLVIAARRLQ*GLPCAET 540
>gb|CW438564.1|CW438564 fsbb001f156e04k0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f156e04, DNA
sequence
Length = 758
Score = 66.6 bits (139), Expect(4) = 4e-031
Identities = 26/29 (89%), Positives = 27/29 (93%)
Frame = -2 / +3
Query: 339 TFIPFLSPNCSAKKCWCRLMASREVSPGY 253
TFIPFLSPNCSA KC CRLMASR+VSPGY
Sbjct: 636 TFIPFLSPNCSAIKCSCRLMASRKVSPGY 722
Score = 66.6 bits (139), Expect(4) = 6e-030
Identities = 26/29 (89%), Positives = 28/29 (96%)
Frame = +2 / -2
Query: 254 YPGETSREAIKRHQHFLALQLGLKKGMKV 340
YPGET REAIKRH+HF+ALQLGLKKGMKV
Sbjct: 721 YPGETFREAIKRHEHFIALQLGLKKGMKV 635
Score = 56.1 bits (116), Expect(4) = 4e-024
Identities = 24/30 (80%), Positives = 26/30 (86%)
Frame = -1 / +2
Query: 340 NLHTLLEPQL*CQKMLVPLNGFTGSLTRIS 251
NLH LLEPQL*C KML+PL+GFT SLT IS
Sbjct: 635 NLHPLLEPQL*CNKMLMPLDGFTESLTWIS 724
Score = 52.8 bits (109), Expect(4) = 6e-030
Identities = 19/24 (79%), Positives = 19/24 (79%)
Frame = +1 / -2
Query: 337 GFGCGMWNWGTFNRNRQIQLNIDN 408
G GCGMWNWGTFNRN QIQ I N
Sbjct: 409 GSGCGMWNWGTFNRNCQIQAMIHN 338
Score = 51.9 bits (107), Expect(4) = 9e-023
Identities = 22/29 (75%), Positives = 23/29 (79%)
Frame = +3 / -3
Query: 252 DIRVRLPVKPLSGTSIFWHYNWGSRRV*R 338
DI+VRL VKP SG SI HYNWGSRR *R
Sbjct: 723 DIQVRLSVKPSSGMSILLHYNWGSRRG*R 637
Score = 51.5 bits (106), Expect(4) = 4e-031
Identities = 18/25 (72%), Positives = 24/25 (96%)
Frame = -3 / +3
Query: 407 LSMLS*IWRFLLKVPQFHIPHPKPS 333
L +++*IW+FLLKVPQFHIPHP+P+
Sbjct: 339 LWIIA*IWQFLLKVPQFHIPHPEPA 413
Score = 49.6 bits (102), Expect(4) = 4e-024
Identities = 19/24 (79%), Positives = 22/24 (91%)
Frame = -2 / +2
Query: 408 VIDVELNLAISIKGPPIPHPTSKT 337
++D LNLAISIKGPPIPHPTS+T
Sbjct: 338 IVDHCLNLAISIKGPPIPHPTSRT 409
Score = 48.7 bits (100), Expect(4) = 9e-023
Identities = 20/24 (83%), Positives = 22/24 (91%)
Frame = +2 / -3
Query: 320 LKKGMKVLDVGCGIGGPLIEIARF 391
LK ++VLDVGCGIGGPLIEIARF
Sbjct: 426 LKLLLQVLDVGCGIGGPLIEIARF 355
Score = 41.4 bits (84), Expect(4) = 4e-031
Identities = 16/22 (72%), Positives = 18/22 (81%)
Frame = -3 / +3
Query: 458 K*APCPLRCGSHCCSVQLSMLS 393
K* PCPLR GS C S+QLS+LS
Sbjct: 186 K*KPCPLRSGSRCSSIQLSLLS 251
Score = 39.6 bits (80), Expect(4) = 4e-024
Identities = 17/22 (77%), Positives = 20/22 (90%)
Frame = -1 / +1
Query: 457 NELLAP*DVVVIVVQSSYRC*A 392
N+ LAP*D+VV+VVQSSY C*A
Sbjct: 187 NKNLAP*DLVVVVVQSSYHC*A 252
Score = 38.2 bits (77), Expect(4) = 6e-030
Identities = 15/24 (62%), Positives = 18/24 (75%)
Frame = +1 / -2
Query: 388 IQLNIDNWTEQQ*LPHLKGQGAHF 459
+QL+ DNW E Q LP LKGQG +F
Sbjct: 256 LQLSNDNWIELQRLPDLKGQGFYF 185
Score = 37.3 bits (75), Expect(4) = 9e-023
Identities = 16/22 (72%), Positives = 16/22 (72%)
Frame = +2 / -3
Query: 392 SSTSITGLNNNDYHISRGKELI 457
SS ITGLN NDY ISRGK I
Sbjct: 252 SSAMITGLNYNDYQISRGKVFI 187
Score = 34.1 bits (68), Expect(4) = 4e-031
Identities = 12/16 (75%), Positives = 14/16 (87%)
Frame = -2 / +1
Query: 495 FKKQHCSLKPAEEMSS 448
FKK+HCS KPAE+ SS
Sbjct: 64 FKKEHCSFKPAEKTSS 111
Score = 31.8 bits (63), Expect(4) = 6e-030
Identities = 12/17 (70%), Positives = 15/17 (88%)
Frame = +2 / -1
Query: 446 KELISSAGLSEQCCFLK 496
+EL+ SAGL+EQC FLK
Sbjct: 113 QELVFSAGLNEQCSFLK 63
Score = 26.7 bits (52), Expect(4) = 9e-023
Identities = 12/17 (70%), Positives = 12/17 (70%)
Frame = +1 / -3
Query: 448 GAHFFSRFERAMLLLEG 498
GA FF E AMLLLEG
Sbjct: 111 GARFFGWLE*AMLLLEG 61
Score = 24.0 bits (46), Expect(4) = 4e-024
Identities = 11/17 (64%), Positives = 12/17 (70%)
Frame = -1 / +3
Query: 496 LQEAALLAQTC*RNELL 446
LQE ALL Q +NELL
Sbjct: 63 LQEGALLIQASRKNELL 113
>gb|CW479900.1|CW479900 fsbb001f238k14f0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f238k14, DNA
sequence
Length = 779
Score = 79.4 bits (167), Expect(4) = 9e-029
Identities = 28/29 (96%), Positives = 28/29 (96%)
Frame = -1 / +1
Query: 577 IWCMTCSLYCIGRIKGVIRYWHVHEVPLQ 491
IWCMTCSLYCIGRIKGVI YWHVHEVPLQ
Sbjct: 328 IWCMTCSLYCIGRIKGVISYWHVHEVPLQ 414
Score = 70.3 bits (147), Expect(4) = 7e-027
Identities = 26/30 (86%), Positives = 30/30 (100%)
Frame = +2 / -3
Query: 491 LKGDFMDMPISDNTFDAAYAIQATCHAPDA 580
L+GDFM+MPI+DNTFDAAYAI+ATCHAPDA
Sbjct: 414 LQGDFMNMPIADNTFDAAYAIEATCHAPDA 325
Score = 68.9 bits (144), Expect(4) = 3e-023
Identities = 26/33 (78%), Positives = 29/33 (87%)
Frame = +1 / -2
Query: 481 MLLLEGGLHGHANI**HL*CGLCNTGYMSCTRC 579
+L++ GGLH HAN **HL*CGLCN GYMSCTRC
Sbjct: 424 LLVIAGGLHEHANS**HL*CGLCNRGYMSCTRC 326
Score = 60.6 bits (126), Expect(4) = 5e-021
Identities = 21/23 (91%), Positives = 23/23 (100%)
Frame = -3 / +3
Query: 578 HLVHDM*PVLHRPHQRCYQILAC 510
HLVHDM*P+LHRPHQRCYQ+LAC
Sbjct: 327 HLVHDM*PLLHRPHQRCYQLLAC 395
Score = 38.2 bits (77), Expect(4) = 9e-029
Identities = 14/16 (87%), Positives = 16/16 (100%)
Frame = -2 / +3
Query: 495 FKKQHCSLKPAEEMSS 448
FKKQHCSLKPAE++SS
Sbjct: 504 FKKQHCSLKPAEKISS 551
Score = 38.2 bits (77), Expect(4) = 7e-027
Identities = 15/17 (88%), Positives = 16/17 (94%)
Frame = +2 / -2
Query: 446 KELISSAGLSEQCCFLK 496
+ELI SAGLSEQCCFLK
Sbjct: 553 QELIFSAGLSEQCCFLK 503
Score = 35.4 bits (71), Expect(4) = 3e-023
Identities = 15/17 (88%), Positives = 16/17 (94%)
Frame = +1 / -1
Query: 448 GAHFFSRFERAMLLLEG 498
GA+FFSRFE AMLLLEG
Sbjct: 551 GANFFSRFE*AMLLLEG 501
Score = 34.5 bits (69), Expect(4) = 7e-027
Identities = 14/19 (73%), Positives = 15/19 (78%)
Frame = +2 / -2
Query: 392 SSTSITGLNNNDYHISRGK 448
SS I GLN+NDY ISRGK
Sbjct: 691 SSALIPGLNSNDYQISRGK 635
Score = 33.6 bits (67), Expect(4) = 7e-027
Identities = 13/13 (100%), Positives = 13/13 (100%)
Frame = +2 / -1
Query: 581 QGVYSEVYRVLKP 619
QGVYSEVYRVLKP
Sbjct: 212 QGVYSEVYRVLKP 174
Score = 32.7 bits (65), Expect(4) = 9e-029
Identities = 14/19 (73%), Positives = 17/19 (89%)
Frame = -1 / +2
Query: 448 LAP*DVVVIVVQSSYRC*A 392
LAP*D+VV+ VQS Y+C*A
Sbjct: 635 LAP*DLVVVGVQSRYQC*A 691
Score = 32.7 bits (65), Expect(4) = 9e-029
Identities = 12/13 (92%), Positives = 13/13 (100%)
Frame = -1 / +3
Query: 619 RFQHTVDLTIDAL 581
RFQHTVDLT+DAL
Sbjct: 174 RFQHTVDLTVDAL 212
Score = 32.2 bits (64), Expect(4) = 5e-021
Identities = 12/19 (63%), Positives = 15/19 (78%)
Frame = -3 / +1
Query: 449 PCPLRCGSHCCSVQLSMLS 393
PCPLR GS S+Q+S+LS
Sbjct: 634 PCPLRSGSRWSSIQVSVLS 690
Score = 32.2 bits (64), Expect(4) = 5e-021
Identities = 12/18 (66%), Positives = 13/18 (72%)
Frame = -3 / +2
Query: 617 VSAHGRPHYRRLVHLVHD 564
VSAHGRPH RRL + D
Sbjct: 176 VSAHGRPHCRRLAAITRD 229
Score = 32.2 bits (64), Expect(4) = 3e-023
Identities = 13/23 (56%), Positives = 15/23 (65%)
Frame = +1 / -1
Query: 394 LNIDNWTEQQ*LPHLKGQGAHFF 462
L+ D W E Q LP LKGQG F+
Sbjct: 689 LSTDTWIELQRLPDLKGQGFLFY 621
Score = 31.3 bits (62), Expect(4) = 5e-021
Identities = 14/17 (82%), Positives = 15/17 (88%)
Frame = -1 / +2
Query: 496 LQEAALLAQTC*RNELL 446
LQEAALL QTC*+N LL
Sbjct: 503 LQEAALLTQTC*KN*LL 553
Score = 27.2 bits (53), Expect(4) = 3e-023
Identities = 12/20 (60%), Positives = 13/20 (65%)
Frame = +1 / -3
Query: 559 YMSCTRCTRRL**GLPCAET 618
+ S RRL *GLPCAET
Sbjct: 234 FSSLVIAARRLQ*GLPCAET 175
>gb|CW246584.1|CW246584 104_707_11221402_148_35087_039 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11221402, DNA
sequence
Length = 634
Score = 73.9 bits (155), Expect(2) = 5e-028
Identities = 28/49 (57%), Positives = 36/49 (73%)
Frame = +2 / -2
Query: 473 SEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVLKP 619
S +C + G+F+ MP D +FD AY+I+ATCHAP Q VY EV+RVLKP
Sbjct: 294 SPRCEVVCGNFLSMPFPDGSFDGAYSIEATCHAPRLQDVYGEVFRVLKP 148
Score = 71.6 bits (150), Expect(2) = 5e-028
Identities = 28/76 (36%), Positives = 46/76 (60%)
Frame = +2 / -2
Query: 260 GETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYHIS 439
G + R+A + H+ +A LG + G ++LDVGCG+GGP+ IA S +++ G+ N+Y ++
Sbjct: 510 GRSHRDATRVHEERVADLLGARPGHRLLDVGCGVGGPMRAIAAHSGSNVVGITINEYQVN 331
Query: 440 RGKELISSAGLSEQCC 487
R + AGL C
Sbjct: 330 RARAHNRKAGLDSPRC 283
>gb|CW472280.1|CW472280 fsbb001f227c06f0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f227c06, DNA
sequence
Length = 389
Score = 73.9 bits (155), Expect(2) = 3e-025
Identities = 28/49 (57%), Positives = 36/49 (73%)
Frame = +2 / -3
Query: 473 SEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVLKP 619
S +C + G+F+ MP D +FD AY+I+ATCHAP Q VY EV+RVLKP
Sbjct: 222 SPRCEVVCGNFLSMPFPDGSFDGAYSIEATCHAPRLQDVYGEVFRVLKP 76
Score = 62.0 bits (129), Expect(2) = 3e-025
Identities = 23/58 (39%), Positives = 36/58 (62%)
Frame = +2 / -3
Query: 314 LGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYHISRGKELISSAGLSEQCC 487
LG + G ++LDVGCG+GGP+ IA S +++ G+ N+Y ++R + AGL C
Sbjct: 384 LGARPGHRLLDVGCGVGGPMRAIAAHSGSNVVGITINEYQVNRARAHNRKAGLDSPRC 211
>gb|CW321502.1|CW321502 104_814_11475589_116_35934_049 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11475589, DNA
sequence
Length = 673
Score = 68.4 bits (143), Expect(4) = 5e-023
Identities = 27/38 (71%), Positives = 32/38 (84%)
Frame = +2 / +3
Query: 491 LKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVY 604
L+GDFM+MPI+DNTFDAA+AI ATCHAPDA + S Y
Sbjct: 333 LQGDFMNMPIADNTFDAAFAIDATCHAPDAVCLTSYFY 446
Score = 67.0 bits (140), Expect(4) = 2e-021
Identities = 24/38 (63%), Positives = 30/38 (78%)
Frame = -1 / -3
Query: 604 VDLTIDALCIWCMTCSLYCIGRIKGVIRYWHVHEVPLQ 491
+++ + CIWC+T S+YC GRIKGVI Y HVHEVPLQ
Sbjct: 446 IEVRRETYCIWCVTRSIYCKGRIKGVISYRHVHEVPLQ 333
Score = 40.0 bits (81), Expect(4) = 2e-021
Identities = 16/22 (72%), Positives = 18/22 (81%)
Frame = -3 / -1
Query: 458 K*APCPLRCGSHCCSVQLSMLS 393
K* P PLR GSHC S+QLS+LS
Sbjct: 112 K*KPFPLRSGSHCSSIQLSLLS 47
Score = 38.6 bits (78), Expect(4) = 5e-023
Identities = 15/24 (62%), Positives = 19/24 (79%)
Frame = +1 / +3
Query: 388 IQLNIDNWTEQQ*LPHLKGQGAHF 459
+QL+ DNW E Q*LP LKG+G +F
Sbjct: 42 LQLSNDNWIELQ*LPDLKGKGFYF 113
Score = 33.1 bits (66), Expect(4) = 5e-023
Identities = 13/17 (76%), Positives = 15/17 (88%)
Frame = +2 / +3
Query: 446 KELISSAGLSEQCCFLK 496
+EL+ SAGLSEQC FLK
Sbjct: 183 QELVFSAGLSEQCSFLK 233
Score = 25.8 bits (50), Expect(4) = 2e-021
Identities = 10/12 (83%), Positives = 10/12 (83%)
Frame = -3 / -2
Query: 617 VSAHGRPHYRRL 582
VSAHGR H RRL
Sbjct: 576 VSAHGRSHCRRL 541
Score = 24.9 bits (48), Expect(4) = 2e-021
Identities = 11/19 (57%), Positives = 13/19 (68%)
Frame = -1 / -3
Query: 496 LQEAALLAQTC*RNELLAP 440
LQE ALL Q ++ELL P
Sbjct: 233 LQEGALLTQASRKDELLQP 177
Score = 23.1 bits (44), Expect(4) = 5e-023
Identities = 10/12 (83%), Positives = 10/12 (83%)
Frame = +1 / +2
Query: 583 RRL**GLPCAET 618
RRL * LPCAET
Sbjct: 542 RRLQ*DLPCAET 577
>gb|CW144783.1|CW144783 104_537_11138124_116_34973_046 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11138124, DNA
sequence
Length = 682
Score = 52.8 bits (109), Expect(4) = 7e-022
Identities = 19/24 (79%), Positives = 19/24 (79%)
Frame = +1 / -2
Query: 337 GFGCGMWNWGTFNRNRQIQLNIDN 408
G GCGMWNWGTFNRN QIQ I N
Sbjct: 489 GSGCGMWNWGTFNRNCQIQAMIHN 418
Score = 38.6 bits (78), Expect(4) = 7e-022
Identities = 15/24 (62%), Positives = 19/24 (79%)
Frame = +1 / -2
Query: 388 IQLNIDNWTEQQ*LPHLKGQGAHF 459
+QL+ DNW E Q*LP LKG+G +F
Sbjct: 336 LQLSNDNWIELQ*LPDLKGKGFYF 265
Score = 34.5 bits (69), Expect(4) = 7e-022
Identities = 12/15 (80%), Positives = 15/15 (100%)
Frame = +2 / -2
Query: 491 LKGDFMDMPISDNTF 535
L+GDFM+MPI+DNTF
Sbjct: 45 LQGDFMNMPIADNTF 1
Score = 33.1 bits (66), Expect(4) = 7e-022
Identities = 13/17 (76%), Positives = 15/17 (88%)
Frame = +2 / -2
Query: 446 KELISSAGLSEQCCFLK 496
+EL+ SAGLSEQC FLK
Sbjct: 195 QELVFSAGLSEQCSFLK 145
>gb|CL178987.1|CL178987 104_387_10894976_148_31905_272 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10894976, DNA
sequence
Length = 473
Score = 51.5 bits (106), Expect(4) = 1e-021
Identities = 18/25 (72%), Positives = 24/25 (96%)
Frame = -3 / +1
Query: 407 LSMLS*IWRFLLKVPQFHIPHPKPS 333
L +++*IW+FLLKVPQFHIPHP+P+
Sbjct: 388 LWIIA*IWQFLLKVPQFHIPHPEPA 462
Score = 41.4 bits (84), Expect(4) = 1e-021
Identities = 16/22 (72%), Positives = 18/22 (81%)
Frame = -3 / +1
Query: 458 K*APCPLRCGSHCCSVQLSMLS 393
K* PCPLR GS C S+QLS+LS
Sbjct: 235 K*KPCPLRSGSRCSSIQLSLLS 300
Score = 34.1 bits (68), Expect(4) = 1e-021
Identities = 12/16 (75%), Positives = 14/16 (87%)
Frame = -2 / +2
Query: 495 FKKQHCSLKPAEEMSS 448
FKK+HCS KPAE+ SS
Sbjct: 113 FKKEHCSFKPAEKTSS 160
Score = 31.3 bits (62), Expect(4) = 1e-021
Identities = 9/10 (90%), Positives = 10/10 (100%)
Frame = -1 / +2
Query: 520 YWHVHEVPLQ 491
YWHVHE+PLQ
Sbjct: 2 YWHVHEIPLQ 31
>gb|CN146201.1|CN146201 WOUND1_38_C11.g1_A002 Wounded leaves Sorghum bicolor cDNA clone
WOUND1_38_C11_A002 5', mRNA sequence
Length = 607
Score = 56.5 bits (117), Expect(3) = 9e-021
Identities = 23/36 (63%), Positives = 26/36 (72%)
Frame = +3 / +1
Query: 93 HQVMAKSGALQLARSTGGSISKDQVKSAVDQYVKYH 200
H M+KSGAL LA GG I K+ VKSAVD+Y KYH
Sbjct: 301 HYTMSKSGALDLASGLGGKIDKEGVKSAVDEYEKYH 408
Score = 46.4 bits (95), Expect(3) = 9e-021
Identities = 18/22 (81%), Positives = 21/22 (95%)
Frame = +2 / +1
Query: 260 GETSREAIKRHQHFLALQLGLK 325
GE+ RE+IKRH+HFLALQLGLK
Sbjct: 541 GESLRESIKRHEHFLALQLGLK 606
Score = 35.4 bits (71), Expect(3) = 9e-021
Identities = 11/17 (64%), Positives = 17/17 (100%)
Frame = +1 / +1
Query: 208 HGGKQESRKSNYTDLIS 258
+GGK+E+RKSNYTD+++
Sbjct: 415 YGGKEEARKSNYTDMVN 465
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 799,521,062
Number of Sequences: 832831
Number of extensions: 11417347
Number of successful extensions: 99166
Number of sequences better than 1.0e-020: 17
length of database: 163,786,556
effective HSP length: 53
effective length of database: 119,646,513
effective search space used: 18186269976
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)