TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 33531.2.1
(724 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BZ343485.1|BZ343485 ho55h04.b2 WGS-SbicolorF (JM107 adap... 67 5e-029
gb|CW305356.1|CW305356 104_790_11466270_148_37437_021 Sorgh... 67 2e-027
gb|CW020744.1|CW020744 104_109_10409144_116_30500 Sorghum m... 67 3e-027
>gb|BZ343485.1|BZ343485 ho55h04.b2 WGS-SbicolorF (JM107 adapted methyl filtered) Sorghum
bicolor genomic clone ho55h04 5', DNA sequence
Length = 640
Score = 66.6 bits (139), Expect(4) = 5e-029
Identities = 25/26 (96%), Positives = 25/26 (96%)
Frame = -3 / +1
Query: 356 SCASPGCRCSRCERATSSSSGTPPVR 279
SCASPGCRCSRCER TSSSSGTPPVR
Sbjct: 166 SCASPGCRCSRCERPTSSSSGTPPVR 243
Score = 66.1 bits (138), Expect(5) = 7e-028
Identities = 25/30 (83%), Positives = 28/30 (93%)
Frame = +2 / -1
Query: 269 DGDDEQAECLMRRTLLAHTDYIYTQGKHN* 358
+G DE+ ECLMRRTL+AHTDYIYTQGKHN*
Sbjct: 253 EGGDEREECLMRRTLVAHTDYIYTQGKHN* 164
Score = 55.6 bits (115), Expect(4) = 5e-029
Identities = 20/25 (80%), Positives = 20/25 (80%)
Frame = -1 / +2
Query: 118 WLRPCRLPPGSAGADEEEQSCYHHE 44
W RPC LPP SAGADEEEQSCY E
Sbjct: 527 WFRPCCLPPSSAGADEEEQSCYDEE 601
Score = 46.4 bits (95), Expect(5) = 7e-028
Identities = 20/25 (80%), Positives = 22/25 (88%)
Frame = +3 / -2
Query: 54 *QLCSSSSAPALPGGKRQGRSQDPR 128
*QLCSSSSAPAL GGK+QGR+Q R
Sbjct: 591 *QLCSSSSAPALLGGKQQGRNQQDR 517
Score = 35.9 bits (72), Expect(5) = 7e-028
Identities = 19/48 (39%), Positives = 25/48 (52%)
Frame = +2 / -1
Query: 140 QQLRADVSISVVAGHEKKSGSSVAVVEMRRQQEPEAVSECEDDDGDDE 283
Q A VS + A +KSGS+ V M R + E +SECE D +E
Sbjct: 373 QLQHAAVSAAGGAAGHEKSGSAAGVEIMHRDEPEEMMSECEGGDEREE 230
Score = 35.4 bits (71), Expect(4) = 5e-029
Identities = 16/34 (47%), Positives = 20/34 (58%)
Frame = -1 / +2
Query: 262 LTFTHRLRLLLPTHFHHGH**ATLLLMTSNDGDG 161
LTF H L L+ H+ H *ATLL+ +S G G
Sbjct: 251 LTFAHHLLRLISMHYFHPGR*ATLLMASSTTGGG 352
Score = 29.0 bits (57), Expect(4) = 5e-029
Identities = 9/9 (100%), Positives = 9/9 (100%)
Frame = -3 / +2
Query: 497 RHCRTSGWL 471
RHCRTSGWL
Sbjct: 86 RHCRTSGWL 112
Score = 25.4 bits (49), Expect(5) = 7e-028
Identities = 7/16 (43%), Positives = 12/16 (75%)
Frame = +1 / -3
Query: 517 VLKYIYLVFSFLQFCQ 564
+++Y+ F FLQFC+
Sbjct: 68 IVQYLVFFFPFLQFCE 21
Score = 24.9 bits (48), Expect(5) = 7e-028
Identities = 10/10 (100%), Positives = 10/10 (100%)
Frame = +2 / -3
Query: 470 LANLKFYSAV 499
LANLKFYSAV
Sbjct: 113 LANLKFYSAV 84
>gb|CW305356.1|CW305356 104_790_11466270_148_37437_021 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11466270, DNA
sequence
Length = 712
Score = 66.6 bits (139), Expect(5) = 1e-026
Identities = 25/26 (96%), Positives = 25/26 (96%)
Frame = -3 / -2
Query: 356 SCASPGCRCSRCERATSSSSGTPPVR 279
SCASPGCRCSRCER TSSSSGTPPVR
Sbjct: 252 SCASPGCRCSRCERPTSSSSGTPPVR 175
Score = 66.1 bits (138), Expect(6) = 2e-027
Identities = 25/30 (83%), Positives = 28/30 (93%)
Frame = +2 / +3
Query: 269 DGDDEQAECLMRRTLLAHTDYIYTQGKHN* 358
+G DE+ ECLMRRTL+AHTDYIYTQGKHN*
Sbjct: 165 EGGDEREECLMRRTLVAHTDYIYTQGKHN* 254
Score = 35.9 bits (72), Expect(6) = 2e-027
Identities = 19/48 (39%), Positives = 25/48 (52%)
Frame = +2 / +3
Query: 140 QQLRADVSISVVAGHEKKSGSSVAVVEMRRQQEPEAVSECEDDDGDDE 283
Q A VS + A +KSGS+ V M R + E +SECE D +E
Sbjct: 45 QLQHAAVSAAGGAAGHEKSGSAAGVEIMHRDEPEEMMSECEGGDEREE 188
Score = 35.4 bits (71), Expect(5) = 1e-026
Identities = 16/34 (47%), Positives = 20/34 (58%)
Frame = -1 / -3
Query: 262 LTFTHRLRLLLPTHFHHGH**ATLLLMTSNDGDG 161
LTF H L L+ H+ H *ATLL+ +S G G
Sbjct: 167 LTFAHHLLRLISMHYFHPGR*ATLLMASSTTGGG 66
Score = 32.7 bits (65), Expect(6) = 2e-027
Identities = 14/24 (58%), Positives = 15/24 (62%)
Frame = +2 / +2
Query: 542 FPFCSSARLXIIFIYTSLITPXXF 613
FPF L IIF+YTSLIT F
Sbjct: 374 FPFLQFCELWIIFMYTSLITSVIF 445
Score = 31.3 bits (62), Expect(5) = 1e-026
Identities = 11/15 (73%), Positives = 14/15 (93%)
Frame = -2 / -3
Query: 654 IWIIYCT*SREADGK 610
+WI+Y T*+READGK
Sbjct: 485 LWIVYRT*AREADGK 441
Score = 30.4 bits (60), Expect(5) = 1e-026
Identities = 15/30 (50%), Positives = 18/30 (60%)
Frame = -2 / -2
Query: 630 SREADGKXXGVISEVYMKIIXSLAELQKGK 541
S + K VI+EVYMKII S +KGK
Sbjct: 462 SSRSGRKITLVINEVYMKIIHSSQNCRKGK 373
Score = 29.0 bits (57), Expect(5) = 1e-026
Identities = 9/9 (100%), Positives = 9/9 (100%)
Frame = -3 / -3
Query: 497 RHCRTSGWL 471
RHCRTSGWL
Sbjct: 332 RHCRTSGWL 306
Score = 27.2 bits (53), Expect(6) = 2e-027
Identities = 10/14 (71%), Positives = 12/14 (85%)
Frame = +2 / +1
Query: 611 FPSASRDHVQYIIH 652
FPSASR +V+Y IH
Sbjct: 442 FPSASRAYVRYTIH 483
Score = 25.4 bits (49), Expect(6) = 2e-027
Identities = 7/16 (43%), Positives = 12/16 (75%)
Frame = +1 / +2
Query: 517 VLKYIYLVFSFLQFCQ 564
+++Y+ F FLQFC+
Sbjct: 350 IVQYLVFFFPFLQFCE 397
Score = 24.9 bits (48), Expect(6) = 2e-027
Identities = 10/10 (100%), Positives = 10/10 (100%)
Frame = +2 / +2
Query: 470 LANLKFYSAV 499
LANLKFYSAV
Sbjct: 305 LANLKFYSAV 334
>gb|CW020744.1|CW020744 104_109_10409144_116_30500 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 10409144, DNA
sequence
Length = 661
Score = 66.6 bits (139), Expect(4) = 3e-027
Identities = 25/26 (96%), Positives = 25/26 (96%)
Frame = -3 / +1
Query: 356 SCASPGCRCSRCERATSSSSGTPPVR 279
SCASPGCRCSRCER TSSSSGTPPVR
Sbjct: 76 SCASPGCRCSRCERPTSSSSGTPPVR 153
Score = 66.1 bits (138), Expect(3) = 8e-023
Identities = 25/30 (83%), Positives = 28/30 (93%)
Frame = +2 / -1
Query: 269 DGDDEQAECLMRRTLLAHTDYIYTQGKHN* 358
+G DE+ ECLMRRTL+AHTDYIYTQGKHN*
Sbjct: 163 EGGDEREECLMRRTLVAHTDYIYTQGKHN* 74
Score = 55.6 bits (115), Expect(4) = 3e-027
Identities = 20/25 (80%), Positives = 20/25 (80%)
Frame = -1 / +2
Query: 118 WLRPCRLPPGSAGADEEEQSCYHHE 44
W RPC LPP SAGADEEEQSCY E
Sbjct: 437 WFRPCCLPPSSAGADEEEQSCYDEE 511
Score = 46.4 bits (95), Expect(3) = 8e-023
Identities = 20/25 (80%), Positives = 22/25 (88%)
Frame = +3 / -2
Query: 54 *QLCSSSSAPALPGGKRQGRSQDPR 128
*QLCSSSSAPAL GGK+QGR+Q R
Sbjct: 501 *QLCSSSSAPALLGGKQQGRNQQDR 427
Score = 35.9 bits (72), Expect(3) = 8e-023
Identities = 19/48 (39%), Positives = 25/48 (52%)
Frame = +2 / -1
Query: 140 QQLRADVSISVVAGHEKKSGSSVAVVEMRRQQEPEAVSECEDDDGDDE 283
Q A VS + A +KSGS+ V M R + E +SECE D +E
Sbjct: 283 QLQHAAVSAAGGAAGHEKSGSAAGVEIMHRDEPEEMMSECEGGDEREE 140
Score = 35.4 bits (71), Expect(4) = 3e-027
Identities = 16/34 (47%), Positives = 20/34 (58%)
Frame = -1 / +2
Query: 262 LTFTHRLRLLLPTHFHHGH**ATLLLMTSNDGDG 161
LTF H L L+ H+ H *ATLL+ +S G G
Sbjct: 161 LTFAHHLLRLISMHYFHPGR*ATLLMASSTTGGG 262
Score = 23.1 bits (44), Expect(4) = 3e-027
Identities = 7/7 (100%), Positives = 7/7 (100%)
Frame = -3 / +2
Query: 491 CRTSGWL 471
CRTSGWL
Sbjct: 2 CRTSGWL 22
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 847,241,121
Number of Sequences: 832831
Number of extensions: 12328630
Number of successful extensions: 148087
Number of sequences better than 1.0e-020: 3
length of database: 163,786,556
effective HSP length: 54
effective length of database: 118,813,682
effective search space used: 22099344852
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)