TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3115190.2.1
(656 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CF484914.1|CF484914 POL1_28_G06.b1_A002 Pollen Sorghum b... 90 4e-042
gb|CW198850.1|CW198850 104_623_11183195_116_36840_036 Sorgh... 84 3e-040
gb|CW401009.1|CW401009 fsbb001f091j18f0 Sorghum methylation... 57 9e-027
gb|BZ350471.1|BZ350471 ht56g09.g1 WGS-SbicolorF (JM107 adap... 57 2e-022
>gb|CF484914.1|CF484914 POL1_28_G06.b1_A002 Pollen Sorghum bicolor cDNA clone
POL1_28_G06_A002 3', mRNA sequence
Length = 601
Score = 90.0 bits (190), Expect(3) = 4e-042
Identities = 34/38 (89%), Positives = 34/38 (89%)
Frame = -3 / +1
Query: 597 AKVIPKPYAEEGVWKNWVWHTEDDLFMNGAIFNPSGGA 484
AKVI K YAEEG WKNWVWHTEDDLFMNGAIF PSGGA
Sbjct: 1 AKVITKHYAEEGEWKNWVWHTEDDLFMNGAIFEPSGGA 114
Score = 83.5 bits (176), Expect(3) = 4e-042
Identities = 32/48 (66%), Positives = 38/48 (79%)
Frame = -3 / +1
Query: 492 GGAPKQVDTNEWVKPKPGTYVTRLTRFSGPLSCCTGKPC*RPVGIRLL 349
G +Q+++NEWVKPKPGTYVTRLTRFSG LSCC KPC* +RL+
Sbjct: 109 GAVQRQINSNEWVKPKPGTYVTRLTRFSGTLSCCMSKPC**LGVVRLI 252
Score = 79.0 bits (166), Expect(3) = 1e-033
Identities = 33/37 (89%), Positives = 34/37 (91%)
Frame = +2 / -3
Query: 485 APPDGLKMAPFMNRSSSVCHTQFFHTPSSA*GLGITL 595
APPDG KMAPFMNRSSSVCHTQFFH+PSSA* L ITL
Sbjct: 113 APPDGSKMAPFMNRSSSVCHTQFFHSPSSA*CLVITL 3
Score = 69.8 bits (146), Expect(3) = 1e-033
Identities = 31/47 (65%), Positives = 34/47 (72%)
Frame = +2 / -3
Query: 353 SLMPTGLQHGLPVQQDSGPEKRVSLVT*VPGLGFTHSLVSTCLGAPP 493
SL QHGL +QQD+ PEKRVSLVT VPGLGFTHSL+ CL P
Sbjct: 248 SLTTPSYQHGLLMQQDNVPEKRVSLVTYVPGLGFTHSLLLICLCTAP 108
Score = 68.9 bits (144), Expect(3) = 2e-026
Identities = 29/36 (80%), Positives = 31/36 (86%)
Frame = -2 / +3
Query: 595 QSDPQALRGRRRVEELGVAHGGRPVHERRHLQSVRR 488
QSD QALRGR VEELGVAHGGRPVHER HL++V R
Sbjct: 3 QSDHQALRGRGGVEELGVAHGGRPVHERSHLRAVGR 110
Score = 57.0 bits (118), Expect(4) = 8e-023
Identities = 30/72 (41%), Positives = 37/72 (51%)
Frame = +1 / -2
Query: 244 CRLISRTTTCDPSLLSSMK*NAASATWHGQAMS*KKQPDAHRPSARLARAAGQWAGEAGE 423
C L+ P L + AA + A + +PD SARLA AAGQ A EA E
Sbjct: 357 CPLLPPIEGLYPFWLQETQTGAALSAMAAAACASSYEPDDAELSARLAHAAGQCAREARE 178
Query: 424 PCNVGSWLGLYP 459
PC+V WLGL+P
Sbjct: 177 PCHVRPWLGLHP 142
Score = 53.8 bits (111), Expect(3) = 2e-026
Identities = 27/45 (60%), Positives = 32/45 (71%)
Frame = -2 / +3
Query: 481 QAGRHQRVGKAQARNLRYKAHPLLRPTVLLHGQAVLKAGGHQAAS 347
+A + QRVG+AQAR++R KAH LL VLLH QAVL A QA S
Sbjct: 120 EADQQQRVGEAQARDVRDKAHALLGHIVLLHEQAVLIARRRQAHS 254
Score = 46.9 bits (96), Expect(4) = 8e-023
Identities = 18/22 (81%), Positives = 18/22 (81%)
Frame = +3 / -1
Query: 531 PPCATPSSSTRLLPRRAWGSLW 596
PPCATPSSST LPR AW SLW
Sbjct: 67 PPCATPSSSTPPLPRSAW*SLW 2
Score = 39.6 bits (80), Expect(3) = 4e-042
Identities = 14/19 (73%), Positives = 16/19 (84%)
Frame = -3 / +2
Query: 120 IYVYSLYNMNHLCLRLV*K 64
+Y+Y LYN NHLCLRLV K
Sbjct: 509 VYIYRLYNRNHLCLRLVEK 565
Score = 37.3 bits (75), Expect(3) = 2e-026
Identities = 14/17 (82%), Positives = 14/17 (82%)
Frame = -1 / +3
Query: 116 TYTVCIT*TTSVCDWSK 66
TYT CIT TTSVCDW K
Sbjct: 513 TYTGCITGTTSVCDWLK 563
Score = 35.9 bits (72), Expect(4) = 8e-023
Identities = 12/15 (80%), Positives = 14/15 (93%)
Frame = +3 / -3
Query: 69 RPIANRGGSCYTNCI 113
+PIA+RGGSCYT CI
Sbjct: 560 QPIADRGGSCYTTCI 516
Score = 35.9 bits (72), Expect(3) = 1e-033
Identities = 15/20 (75%), Positives = 17/20 (85%)
Frame = +1 / -1
Query: 58 YLFLDQSQTEVVHVIQTVYV 117
Y+F +QSQTEVV VIQ VYV
Sbjct: 571 YVFFNQSQTEVVPVIQPVYV 512
Score = 24.4 bits (47), Expect(4) = 8e-023
Identities = 8/10 (80%), Positives = 10/10 (100%)
Frame = +1 / -2
Query: 496 RIEDGAVHEQ 525
R+EDG+VHEQ
Sbjct: 102 RLEDGSVHEQ 73
>gb|CW198850.1|CW198850 104_623_11183195_116_36840_036 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11183195, DNA
sequence
Length = 729
Score = 84.0 bits (177), Expect(3) = 3e-040
Identities = 31/35 (88%), Positives = 31/35 (88%)
Frame = -3 / +2
Query: 588 IPKPYAEEGVWKNWVWHTEDDLFMNGAIFNPSGGA 484
I K YAEEG WKNWVWHTEDDLFMNGAIF PSGGA
Sbjct: 8 ITKHYAEEGEWKNWVWHTEDDLFMNGAIFEPSGGA 112
Score = 83.5 bits (176), Expect(3) = 3e-040
Identities = 32/48 (66%), Positives = 38/48 (79%)
Frame = -3 / +2
Query: 492 GGAPKQVDTNEWVKPKPGTYVTRLTRFSGPLSCCTGKPC*RPVGIRLL 349
G +Q+++NEWVKPKPGTYVTRLTRFSG LSCC KPC* +RL+
Sbjct: 107 GAVQRQINSNEWVKPKPGTYVTRLTRFSGTLSCCMSKPC**LGVVRLI 250
Score = 74.8 bits (157), Expect(3) = 2e-032
Identities = 31/35 (88%), Positives = 32/35 (91%)
Frame = +2 / -1
Query: 485 APPDGLKMAPFMNRSSSVCHTQFFHTPSSA*GLGI 589
APPDG KMAPFMNRSSSVCHTQFFH+PSSA* L I
Sbjct: 111 APPDGSKMAPFMNRSSSVCHTQFFHSPSSA*CLVI 7
Score = 69.8 bits (146), Expect(3) = 2e-032
Identities = 31/47 (65%), Positives = 34/47 (72%)
Frame = +2 / -1
Query: 353 SLMPTGLQHGLPVQQDSGPEKRVSLVT*VPGLGFTHSLVSTCLGAPP 493
SL QHGL +QQD+ PEKRVSLVT VPGLGFTHSL+ CL P
Sbjct: 246 SLTTPSYQHGLLMQQDNVPEKRVSLVTYVPGLGFTHSLLLICLCTAP 106
Score = 65.2 bits (136), Expect(3) = 3e-025
Identities = 27/35 (77%), Positives = 30/35 (85%)
Frame = -2 / +1
Query: 592 SDPQALRGRRRVEELGVAHGGRPVHERRHLQSVRR 488
+D QALRGR VEELGVAHGGRPVHER HL++V R
Sbjct: 4 ADHQALRGRGGVEELGVAHGGRPVHERSHLRAVGR 108
Score = 53.8 bits (111), Expect(3) = 3e-025
Identities = 27/45 (60%), Positives = 32/45 (71%)
Frame = -2 / +1
Query: 481 QAGRHQRVGKAQARNLRYKAHPLLRPTVLLHGQAVLKAGGHQAAS 347
+A + QRVG+AQAR++R KAH LL VLLH QAVL A QA S
Sbjct: 118 EADQQQRVGEAQARDVRDKAHALLGHIVLLHEQAVLIARRRQAHS 252
Score = 39.6 bits (80), Expect(3) = 3e-040
Identities = 14/19 (73%), Positives = 16/19 (84%)
Frame = -3 / +3
Query: 120 IYVYSLYNMNHLCLRLV*K 64
+Y+Y LYN NHLCLRLV K
Sbjct: 507 VYIYRLYNRNHLCLRLVEK 563
Score = 37.3 bits (75), Expect(3) = 3e-025
Identities = 14/17 (82%), Positives = 14/17 (82%)
Frame = -1 / +1
Query: 116 TYTVCIT*TTSVCDWSK 66
TYT CIT TTSVCDW K
Sbjct: 511 TYTGCITGTTSVCDWLK 561
Score = 35.9 bits (72), Expect(3) = 2e-032
Identities = 15/20 (75%), Positives = 17/20 (85%)
Frame = +1 / -2
Query: 58 YLFLDQSQTEVVHVIQTVYV 117
Y+F +QSQTEVV VIQ VYV
Sbjct: 569 YVFFNQSQTEVVPVIQPVYV 510
>gb|CW401009.1|CW401009 fsbb001f091j18f0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f091j18, DNA
sequence
Length = 684
Score = 56.5 bits (117), Expect(3) = 9e-027
Identities = 20/29 (68%), Positives = 22/29 (75%)
Frame = -3 / -3
Query: 573 AEEGVWKNWVWHTEDDLFMNGAIFNPSGG 487
A E WKNWVWH+E+DLFM GA F SGG
Sbjct: 394 APESEWKNWVWHSENDLFMEGANFTVSGG 308
Score = 51.9 bits (107), Expect(3) = 9e-027
Identities = 19/28 (67%), Positives = 24/28 (85%)
Frame = -3 / -3
Query: 456 VKPKPGTYVTRLTRFSGPLSCCTGKPC* 373
+KPK G+YVTRLTR++G L+C GKPC*
Sbjct: 271 IKPKNGSYVTRLTRYAGALACRPGKPC* 188
Score = 50.6 bits (104), Expect(3) = 9e-027
Identities = 19/24 (79%), Positives = 23/24 (95%)
Frame = -3 / -3
Query: 651 APTIISQGNRFIAPPNIAAKVIPK 580
APTIISQGNR+IAPPN+AAK++ K
Sbjct: 475 APTIISQGNRYIAPPNLAAKLVTK 404
>gb|BZ350471.1|BZ350471 ht56g09.g1 WGS-SbicolorF (JM107 adapted methyl filtered) Sorghum
bicolor genomic clone ht56g09 5', DNA sequence
Length = 596
Score = 56.5 bits (117), Expect(3) = 2e-022
Identities = 20/29 (68%), Positives = 22/29 (75%)
Frame = -3 / -1
Query: 573 AEEGVWKNWVWHTEDDLFMNGAIFNPSGG 487
A E WKNWVWH+E+DLFM GA F SGG
Sbjct: 479 APESEWKNWVWHSENDLFMEGANFTVSGG 393
Score = 50.6 bits (104), Expect(3) = 2e-022
Identities = 19/24 (79%), Positives = 23/24 (95%)
Frame = -3 / -1
Query: 651 APTIISQGNRFIAPPNIAAKVIPK 580
APTIISQGNR+IAPPN+AAK++ K
Sbjct: 560 APTIISQGNRYIAPPNLAAKLVTK 489
Score = 36.8 bits (74), Expect(3) = 2e-022
Identities = 13/20 (65%), Positives = 18/20 (90%)
Frame = -3 / -1
Query: 456 VKPKPGTYVTRLTRFSGPLS 397
+KPK G+YVTRLTR++G L+
Sbjct: 356 IKPKNGSYVTRLTRYAGALA 297
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 881,254,668
Number of Sequences: 832831
Number of extensions: 14938361
Number of successful extensions: 177180
Number of sequences better than 1.0e-020: 4
length of database: 163,786,556
effective HSP length: 54
effective length of database: 118,813,682
effective search space used: 19485443848
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)