TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3024030.2.1
(412 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CW181998.1|CW181998 104_597_11163822_148_36661_027 Sorgh... 159 1e-039
>gb|CW181998.1|CW181998 104_597_11163822_148_36661_027 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11163822, DNA
sequence
Length = 722
Score = 159 bits (341), Expect(2) = 1e-039
Identities = 67/96 (69%), Positives = 71/96 (73%)
Frame = +2 / -2
Query: 83 KLKNHFAPVAAECTLDHHDLQFPSLLAFFLTXXXXXXXXXXXYPSAISSRTRRFTHFAFG 262
K K + AE TL HHDLQ PS+LA FLT YP AISS+TRRFTHFAF
Sbjct: 370 KKKLNHLAPVAEWTLGHHDLQLPSILACFLTSRRSNK*RTIRYPIAISSKTRRFTHFAFV 191
Query: 263 EDEMSYEPRTAWTPGMVLICPTANDVPTYSFSTKPC 370
EDEMSYEPRTAWTPG+VLICPTA DVPTYSFSTKPC
Sbjct: 190 EDEMSYEPRTAWTPGIVLICPTAKDVPTYSFSTKPC 83
Score = 118 bits (252), Expect(3) = 6e-037
Identities = 49/65 (75%), Positives = 52/65 (80%)
Frame = -3 / +1
Query: 374 LCRAWWRTSTWAHRLLLDRSGPYLVSKPFEARTTFRLPQTRSG*TSWFCY*WRSDIGFFS 195
L RAW +TS AH LLLDR G YLVSKP EA TF LPQT+SG*TSWFCY*W+SD G FS
Sbjct: 79 LDRAWSKTSMLAHPLLLDR*GLYLVSKPSEAHMTFHLPQTQSG*TSWFCY*WQSDTGLFS 258
Query: 194 TCCFV 180
TCCFV
Sbjct: 259 TCCFV 273
Score = 110 bits (234), Expect(2) = 5e-026
Identities = 48/68 (70%), Positives = 56/68 (82%)
Frame = +1 / -1
Query: 172 HV*TKQQVEKNPISERHQ*QNQEVHPLRVWGRRNVVRASNGLDTRYGPDLSNSKRCAHVL 351
++*TKQQVE NP+S+ HQ*QNQEVHPL V GR NV+ AS+GLDTRY P LSNSK CA++L
Sbjct: 281 YI*TKQQVENNPVSDCHQ*QNQEVHPLCVCGR*NVI*ASDGLDTRYSPYLSNSKGCANIL 102
Query: 352 VLHQALHS 375
V QAL S
Sbjct: 101 VFDQALSS 78
Score = 101 bits (215), Expect(2) = 2e-024
Identities = 36/50 (72%), Positives = 43/50 (86%)
Frame = -1 / +2
Query: 367 GLGGERVRGHIVCCWTDQDHTWCPSRSRLVRHFVFPKREVGEPPGSATDG 218
GLG +RV HI+CCWTD+D+TWCPSR RL+ HF+F KR+VGEPPG ATDG
Sbjct: 86 GLGRKRVCWHILCCWTDKDYTWCPSRPRLI*HFIFHKRKVGEPPGFATDG 235
Score = 53.3 bits (110), Expect(3) = 6e-037
Identities = 19/22 (86%), Positives = 20/22 (90%)
Frame = -2 / +3
Query: 177 DVRKNARRLGNWRSWWSSVHSA 112
DVRK+AR LGNWRSWW SVHSA
Sbjct: 276 DVRKHARMLGNWRSWWPSVHSA 341
Score = 31.3 bits (62), Expect(2) = 2e-024
Identities = 10/13 (76%), Positives = 11/13 (84%)
Frame = -1 / +2
Query: 151 WKLEVMVVKCAFC 113
W+LEVMV KC FC
Sbjct: 302 WQLEVMVAKCPFC 340
Score = 27.6 bits (54), Expect(2) = 5e-026
Identities = 10/33 (30%), Positives = 19/33 (57%)
Frame = +3 / -2
Query: 27 NQTKELTKHLRHSLTRLDRSSKITLHQWQQNAH 125
+++K+LTKHLR S+ + + + +W H
Sbjct: 415 DKSKQLTKHLRRSIKKKKLNHLAPVAEWTLGHH 317
Score = 24.4 bits (47), Expect(2) = 1e-039
Identities = 9/12 (75%), Positives = 9/12 (75%)
Frame = +1 / -3
Query: 25 QTKPKNSLNTYD 60
QT NSLNTYD
Sbjct: 417 QTNQNNSLNTYD 382
Score = 21.7 bits (41), Expect(3) = 6e-037
Identities = 10/21 (47%), Positives = 12/21 (57%)
Frame = -2 / +3
Query: 132 WSSVHSAATGAK*FLSFYLIE 70
W +ATGAK* F+ IE
Sbjct: 318 WWPSVHSATGAK*LSFFFFIE 380
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 540,076,546
Number of Sequences: 832831
Number of extensions: 7333226
Number of successful extensions: 280800
Number of sequences better than 1.0e-020: 1
length of database: 163,786,556
effective HSP length: 52
effective length of database: 120,479,344
effective search space used: 10120264896
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)