TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2921788.2.1
(606 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CD428012.1|CD428012 ETH1_32_G01.b1_A002 Ethylene-treated... 155 2e-056
gb|CD428117.1|CD428117 ETH1_32_G01.g1_A002 Ethylene-treated... 122 5e-042
gb|CD226887.1|CD226887 CCC1_48_D11.g1_A007 Callus culture/c... 116 3e-040
>gb|CD428012.1|CD428012 ETH1_32_G01.b1_A002 Ethylene-treated seedlings Sorghum bicolor cDNA
clone ETH1_32_G01_A002 3', mRNA sequence
Length = 555
Score = 155 bits (334), Expect(4) = 2e-056
Identities = 63/73 (86%), Positives = 64/73 (87%)
Frame = +1 / +2
Query: 175 AWCFSDPEMKRRRRVASYKAYSAEGKVKASFPRGFRWIKAKCSELIHG*TRHLGEILLYW 354
AWCFSDPEMKRRRRVASYKAYS EGKVKASF RGFRWIKAKCSELIHG*TR+LGEIL YW
Sbjct: 119 AWCFSDPEMKRRRRVASYKAYSVEGKVKASFRRGFRWIKAKCSELIHG*TRYLGEILSYW 298
Query: 355 HGARCGYKAREMV 393
G R Y AR MV
Sbjct: 299 DGGRWSYNARRMV 337
Score = 128 bits (275), Expect(4) = 1e-040
Identities = 53/68 (77%), Positives = 56/68 (82%)
Frame = -1 / -3
Query: 393 NHLSRFVAASCTMPIQ*NFTEMPGSAMDELGALGLDPAESPGERCLDFALRRVSLVAGHP 214
NH SR VA T+PI *NFTE+PGSAMDELGALGLDPAE P ERCLDFAL RV LVA HP
Sbjct: 337 NHSSRVVAPPSTIPIG*NFTEIPGSAMDELGALGLDPAEPPAERCLDFALHRVGLVARHP 158
Query: 213 PPPFHLRI 190
PPP HLR+
Sbjct: 157 PPPLHLRV 134
Score = 112 bits (239), Expect(2) = 4e-031
Identities = 53/74 (71%), Positives = 55/74 (74%)
Frame = -2 / -1
Query: 395 QTISRAL*PHLAPCQYNRISPRCRVQPWMSSEHLALIQRNPLGNDALTLPSXXXXXXXXX 216
+TI RAL* HL P Q +RISPR RVQPWMSSEHLALIQRNPL NDALTLPS
Sbjct: 339 RTIRRAL*LHLPPSQ*DRISPRYRVQPWMSSEHLALIQRNPLRNDALTLPSTE*AL*LAT 160
Query: 215 XXXLFISGSLKHHA 174
LFISGSLKHHA
Sbjct: 159 RRRLFISGSLKHHA 118
Score = 102 bits (218), Expect(3) = 9e-032
Identities = 43/70 (61%), Positives = 48/70 (68%)
Frame = +3 / +1
Query: 183 LQRSGDEKXXXXXXXXXXXXXXXSQGIVPQGIPLDQGQVLRAHPWLNPASR*NSIVLAWC 362
LQR GDE+ SQGIVPQG+PLDQGQVLRAHPWLNP SR*NSI+L W
Sbjct: 127 LQRPGDEEAAAGGELQGLLGGGQSQGIVPQGVPLDQGQVLRAHPWLNPVSR*NSILLGWW 306
Query: 363 KMRLQSARDG 392
K+ LQ A +G
Sbjct: 307 KVELQRATNG 336
Score = 90.9 bits (192), Expect(4) = 5e-028
Identities = 39/67 (58%), Positives = 42/67 (62%)
Frame = +2 / +3
Query: 194 RR*KGGGGWPATRLTRRRAKSRHXXXXXXXXXXXXXXXXXMAEPGISVKFYCIGMVQDAA 373
RR*+GGGGW ATR TR RAKSRH MAEPGISVKFY IGMV+ A
Sbjct: 138 RR*RGGGGWRATRPTRWRAKSRHRSAGGSAGSRPSAPSSSMAEPGISVKFYPIGMVEGGA 317
Query: 374 TKRERWF 394
T R+ WF
Sbjct: 318 TTRDEWF 338
Score = 83.5 bits (176), Expect(2) = 4e-022
Identities = 33/45 (73%), Positives = 35/45 (77%)
Frame = -3 / -2
Query: 394 KPSLALCSRILHHANTIEFHRDAGFSHG*ARSTWP*SSGIPWGTM 260
+P +A CS HH N IEFHRD GFSHG*ARSTWP*SSG P GTM
Sbjct: 338 EPFVARCSSTFHHPNRIEFHRDTGFSHG*ARSTWP*SSGTPCGTM 204
Score = 46.4 bits (95), Expect(4) = 1e-040
Identities = 21/32 (65%), Positives = 24/32 (75%)
Frame = -2 / -1
Query: 491 FSYNQGGNKVLYTISEQILFHILRAN*RNNNR 396
F +GG +VLYTISEQ LFHIL AN*R + R
Sbjct: 432 FPITRGGPQVLYTISEQFLFHILGAN*RVHPR 337
Score = 45.5 bits (93), Expect(4) = 2e-056
Identities = 18/22 (81%), Positives = 18/22 (81%)
Frame = +2 / +3
Query: 410 ANSLSKCGREFVLRLCRELCCP 475
ANSL KCGRE VLRLCREL P
Sbjct: 351 ANSLPKCGREIVLRLCRELVAP 416
Score = 45.5 bits (93), Expect(3) = 9e-032
Identities = 22/36 (61%), Positives = 23/36 (63%)
Frame = +3 / +1
Query: 396 SVVISLIRSQNVEENLF*DCVENFVAPLVIGKELDG 503
S + SLIRSQNVEE LF*DCVEN P K G
Sbjct: 337 SRMYSLIRSQNVEEKLF*DCVENLWPPSGYRKRTSG 444
Score = 44.6 bits (91), Expect(4) = 2e-056
Identities = 16/18 (88%), Positives = 18/18 (100%)
Frame = +1 / +2
Query: 70 EVVPYGDRRIDIIVKPPA 123
EVVPYGDRR+DI+VKPPA
Sbjct: 2 EVVPYGDRRLDIVVKPPA 55
Score = 43.2 bits (88), Expect(2) = 4e-031
Identities = 19/23 (82%), Positives = 19/23 (82%)
Frame = -3 / -2
Query: 475 GATKFSTQSQNKFSSTF*ERISE 407
G KFSTQSQN FSSTF ERISE
Sbjct: 416 GGHKFSTQSQNNFSSTFWERISE 348
Score = 42.8 bits (87), Expect(4) = 5e-028
Identities = 19/23 (82%), Positives = 19/23 (82%)
Frame = +1 / +2
Query: 409 R*FALKMWKRICSEIV*RTLLPP 477
R*FA KMWKR CSEIV*RT PP
Sbjct: 350 R*FAPKMWKRNCSEIV*RTCGPP 418
Score = 41.8 bits (85), Expect(2) = 4e-022
Identities = 18/22 (81%), Positives = 18/22 (81%)
Frame = -1 / -3
Query: 474 GQQSSLHNLRTNSLPHFESELA 409
G SSLHNLRT SLPHF SELA
Sbjct: 415 GATSSLHNLRTISLPHFGSELA 350
Score = 31.3 bits (62), Expect(4) = 2e-056
Identities = 15/46 (32%), Positives = 24/46 (52%)
Frame = +2 / +1
Query: 455 CRELCCPPGYRKRTRWRSAEITCRYLPIAPNSVQKMFEEDLNARRK 592
C E PP ++ S+E+ CR L I PN VQ +++ + +K
Sbjct: 394 CVENLWPPSGYRKRTSGSSELACRCLLITPNFVQTCLKKN*ISEKK 531
Score = 27.6 bits (54), Expect(3) = 9e-032
Identities = 13/17 (76%), Positives = 13/17 (76%)
Frame = +3 / +2
Query: 507 LLRLLVATCQLPLILFK 557
LL LLVA C LPLIL K
Sbjct: 446 LLSLLVAAC*LPLILSK 496
Score = 26.3 bits (51), Expect(4) = 1e-040
Identities = 10/14 (71%), Positives = 11/14 (78%)
Frame = -1 / -3
Query: 114 LDDDVDAAVTVGHD 73
LDDDV+A V V HD
Sbjct: 46 LDDDVEAPVAVRHD 5
Score = 25.8 bits (50), Expect(4) = 5e-028
Identities = 10/11 (90%), Positives = 10/11 (90%)
Frame = +1 / +3
Query: 520 LSLPANCP*FC 552
LSLPAN P*FC
Sbjct: 459 LSLPANYP*FC 491
Score = 22.1 bits (42), Expect(4) = 1e-040
Identities = 6/9 (66%), Positives = 7/9 (77%)
Frame = -3 / -2
Query: 148 WWIPAGATS 122
WWIPA A +
Sbjct: 89 WWIPAAAAA 63
Score = 21.7 bits (41), Expect(4) = 5e-028
Identities = 11/18 (61%), Positives = 12/18 (66%)
Frame = +3 / +2
Query: 474 PLVIGKELDGDLLRLLVA 527
PLVIGKEL L L+ A
Sbjct: 416 PLVIGKELVALLSLLVAA 469
>gb|CD428117.1|CD428117 ETH1_32_G01.g1_A002 Ethylene-treated seedlings Sorghum bicolor cDNA
clone ETH1_32_G01_A002 5', mRNA sequence
Length = 454
Score = 122 bits (262), Expect(2) = 5e-042
Identities = 49/52 (94%), Positives = 50/52 (96%)
Frame = +1 / +3
Query: 175 AWCFSDPEMKRRRRVASYKAYSAEGKVKASFPRGFRWIKAKCSELIHG*TRH 330
AWCFSDPEMKRRRRVASYKAYS EGKVKASF RGFRWIKAKCSELIHG*TR+
Sbjct: 297 AWCFSDPEMKRRRRVASYKAYSVEGKVKASFRRGFRWIKAKCSELIHG*TRY 452
Score = 100 bits (212), Expect(3) = 1e-023
Identities = 39/47 (82%), Positives = 41/47 (87%)
Frame = -1 / -3
Query: 330 MPGSAMDELGALGLDPAESPGERCLDFALRRVSLVAGHPPPPFHLRI 190
+PGSAMDELGALGLDPAE P ERCLDFAL RV LVA HPPPP HLR+
Sbjct: 452 IPGSAMDELGALGLDPAEPPAERCLDFALHRVGLVARHPPPPLHLRV 312
Score = 69.3 bits (145), Expect(2) = 5e-042
Identities = 25/27 (92%), Positives = 27/27 (100%)
Frame = +1 / +3
Query: 43 GEYDRPYRNEVVPYGDRRIDIIVKPPA 123
GEYDRPYRNEVVPYGDRR+DI+VKPPA
Sbjct: 153 GEYDRPYRNEVVPYGDRRLDIVVKPPA 233
Score = 26.3 bits (51), Expect(3) = 1e-023
Identities = 10/14 (71%), Positives = 11/14 (78%)
Frame = -1 / -3
Query: 114 LDDDVDAAVTVGHD 73
LDDDV+A V V HD
Sbjct: 224 LDDDVEAPVAVRHD 183
Score = 22.1 bits (42), Expect(3) = 1e-023
Identities = 6/9 (66%), Positives = 7/9 (77%)
Frame = -3 / -2
Query: 148 WWIPAGATS 122
WWIPA A +
Sbjct: 267 WWIPAAAAA 241
>gb|CD226887.1|CD226887 CCC1_48_D11.g1_A007 Callus culture/cell suspension Sorghum bicolor
cDNA clone CCC1_48_D11_A007 5', mRNA sequence
Length = 670
Score = 116 bits (249), Expect(2) = 3e-040
Identities = 46/48 (95%), Positives = 46/48 (95%)
Frame = +1 / +2
Query: 175 AWCFSDPEMKRRRRVASYKAYSAEGKVKASFPRGFRWIKAKCSELIHG 318
AWCFSDPEMKRRRRVASYKAYS EGKVKASF RGFRWIKAKCSELIHG
Sbjct: 263 AWCFSDPEMKRRRRVASYKAYSVEGKVKASFRRGFRWIKAKCSELIHG 406
Score = 91.8 bits (194), Expect(3) = 8e-022
Identities = 36/43 (83%), Positives = 37/43 (86%)
Frame = -1 / -1
Query: 318 AMDELGALGLDPAESPGERCLDFALRRVSLVAGHPPPPFHLRI 190
AMDELGALGLDPAE P ERCLDFAL RV LVA HPPPP HLR+
Sbjct: 406 AMDELGALGLDPAEPPAERCLDFALHRVGLVARHPPPPLHLRV 278
Score = 69.3 bits (145), Expect(2) = 3e-040
Identities = 25/27 (92%), Positives = 27/27 (100%)
Frame = +1 / +2
Query: 43 GEYDRPYRNEVVPYGDRRIDIIVKPPA 123
GEYDRPYRNEVVPYGDRR+DI+VKPPA
Sbjct: 128 GEYDRPYRNEVVPYGDRRLDIVVKPPA 208
Score = 26.3 bits (51), Expect(3) = 8e-022
Identities = 10/14 (71%), Positives = 11/14 (78%)
Frame = -1 / -1
Query: 114 LDDDVDAAVTVGHD 73
LDDDV+A V V HD
Sbjct: 199 LDDDVEAPVAVRHD 158
Score = 24.0 bits (46), Expect(3) = 8e-022
Identities = 7/8 (87%), Positives = 7/8 (87%)
Frame = -3 / -3
Query: 148 WWIPAGAT 125
WWIPA AT
Sbjct: 233 WWIPAAAT 210
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 562,521,534
Number of Sequences: 832831
Number of extensions: 7398780
Number of successful extensions: 74252
Number of sequences better than 1.0e-020: 3
length of database: 163,786,556
effective HSP length: 53
effective length of database: 119,646,513
effective search space used: 17707683924
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)