TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2921719.2.1
(779 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CF488470.1|CF488470 POL1_50_E05.b1_A002 Pollen Sorghum b... 259 1e-081
gb|CD223298.1|CD223298 CCC1_27_E03.b1_A007 Callus culture/c... 259 1e-081
gb|CD223408.1|CD223408 CCC1_27_E03.g1_A007 Callus culture/c... 259 5e-081
gb|CF485791.1|CF485791 POL1_33_E06.g1_A002 Pollen Sorghum b... 254 4e-080
gb|CF485720.1|CF485720 POL1_33_E06.b1_A002 Pollen Sorghum b... 116 6e-025
>gb|CF488470.1|CF488470 POL1_50_E05.b1_A002 Pollen Sorghum bicolor cDNA clone
POL1_50_E05_A002 3', mRNA sequence
Length = 585
Score = 259 bits (559), Expect(3) = 9e-078
Identities = 112/134 (83%), Positives = 112/134 (83%)
Frame = -3 / -2
Query: 456 RN*STEQRRPF*RWKGG*GFKRSWSPPWPPGAHLXXXXXXXXXXXCW*LIGRRNSMMCGS 277
RN*STE RRPF*R KG *GFK WSPP P GAHL CW*LIGRRNSMMCGS
Sbjct: 452 RN*STE*RRPF*R*KGD*GFKHFWSPPLPAGAHLASNCAAAASCCCW*LIGRRNSMMCGS 273
Query: 276 GRWIA*CTHPRLCCTPIARHSFSERRRFLGRSLATSGGRTTCLYS*VSSKYFSEYWI*PM 97
GRWIA*C HPRLCCTPIARHSFSERRRFLGRSLATSGGRTTCLYS*VSSKYFSEYWI*PM
Sbjct: 272 GRWIA*CIHPRLCCTPIARHSFSERRRFLGRSLATSGGRTTCLYS*VSSKYFSEYWI*PM 93
Query: 96 STNASRSTPLGADA 55
STN SRST LGA A
Sbjct: 92 STNTSRSTSLGAGA 51
Score = 258 bits (557), Expect(3) = 1e-081
Identities = 108/136 (79%), Positives = 111/136 (81%)
Frame = +2 / +1
Query: 50 KNASAPRGVDREALVDMGQIQYSEKYFDDTYEYRHVVLPPEVAKLLPKNRLLSENEWRAI 229
K A APR VDRE LVDMGQIQYSEKYFDDTYEYRHVVLPPEVAKLLPKNRLLSENEWRAI
Sbjct: 46 KTAPAPREVDREVLVDMGQIQYSEKYFDDTYEYRHVVLPPEVAKLLPKNRLLSENEWRAI 225
Query: 230 GVQQSRGWVHYAIHRPEPHIMLFRRPINYXXXXXXXXXXXXXXXXPGGQGGDQERLKP*P 409
GVQQSRGW+HYAIHRPEPHIMLFRRPINY P G+GGDQ+ LKP*
Sbjct: 226 GVQQSRGWMHYAIHRPEPHIMLFRRPINYQQQQEAAAAQLLAK*APAGKGGDQKCLKP*S 405
Query: 410 PFHL*KGRRCSVDQFL 457
PFHL*KGRR SVD FL
Sbjct: 406 PFHL*KGRRYSVD*FL 453
Score = 124 bits (266), Expect(3) = 2e-045
Identities = 57/80 (71%), Positives = 61/80 (76%)
Frame = -2 / -1
Query: 457 EELINRTATPLLEMERGLGF*TFLVAALATXXXXXXXXXXXXXXXXLVVDRAAEQHDVRL 278
+ELINR ATPLLEM+RGLGF*TFLVA LA+ LVVDRAAEQHDVRL
Sbjct: 453 QELINRIATPLLEMKRGLGF*TFLVATLASRGSLGEQLRGSRLLLLLVVDRAAEQHDVRL 274
Query: 277 RAVDRVVHPPAALLHSDRAP 218
R VDRVVHPPAALLH+DRAP
Sbjct: 273 RTVDRVVHPPAALLHADRAP 214
Score = 93.6 bits (198), Expect(3) = 3e-033
Identities = 43/79 (54%), Positives = 46/79 (58%)
Frame = +3 / +2
Query: 219 GARSECSRAAGGXXXXXXXXXXXXXXXXXXXXXXXXXXXLPRRCWPSEPPVARAATRNV* 398
GARS CSRAAGG LPR C PSEP +AR ATRNV*
Sbjct: 215 GARSACSRAAGGCTTRSTVRSRTSCCSAARSTTSSSRRRLPRSCSPSEPLLARVATRNV* 394
Query: 399 NPNPLSISKRGVAVLLINS 455
NP+PL ISKRGVA+LLINS
Sbjct: 395 NPSPLFISKRGVAILLINS 451
Score = 91.8 bits (194), Expect(2) = 4e-024
Identities = 44/80 (55%), Positives = 45/80 (56%)
Frame = +1 / +3
Query: 220 ARDRSAAEPRVGALRDXXXXXXXXXXXXXDQLPXXXXXXXXXXXXQVSPRWPGRRPGTFK 399
ARDR AAEPRV ALRD DQLP QVSP W G RP FK
Sbjct: 216 ARDRRAAEPRVDALRDPPSGAAHHAVPPPDQLPAAAGGGCRAVARQVSPCWQGWRPEMFK 395
Query: 400 TLTPFPSLKGASLFC*SIPL 459
TL PF SLKGASLFC* IP+
Sbjct: 396 TLVPFSSLKGASLFC*LIPV 455
Score = 65.7 bits (137), Expect(3) = 1e-025
Identities = 25/34 (73%), Positives = 28/34 (82%)
Frame = -1 / -3
Query: 455 GIDQQNSDAPFRDGKGVRVLNVPGRRPGHRGLTW 354
GI+QQNSDAPFRD KG RVLN+ GR P +GLTW
Sbjct: 451 GINQQNSDAPFRDEKGTRVLNISGRHPCQQGLTW 350
Score = 57.4 bits (119), Expect(3) = 1e-081
Identities = 19/24 (79%), Positives = 22/24 (91%)
Frame = +3 / +3
Query: 489 WSICWCVPCVRGNSCEGTAMC*AV 560
W + WC+PCVRGN+CEGTAMC*AV
Sbjct: 480 WLLGWCLPCVRGNACEGTAMC*AV 551
Score = 50.6 bits (104), Expect(3) = 2e-045
Identities = 20/30 (66%), Positives = 23/30 (76%)
Frame = -1 / -3
Query: 104 DPCPPTLLDQLPLERMRFSRRNAWQESPRG 15
DPCPPTLLDQLPLER+RF R + + RG
Sbjct: 100 DPCPPTLLDQLPLERVRFCRGAPTRVAARG 11
Score = 49.6 bits (102), Expect(3) = 9e-078
Identities = 19/32 (59%), Positives = 22/32 (68%)
Frame = -3 / -1
Query: 558 QPNTWQFLHRNFHEHKAHTSKSTNYTRSFQLT 463
QPNTWQFLHR+FHEHK T+ + S Q T
Sbjct: 549 QPNTWQFLHRHFHEHKGDTTLTARSQVSNQQT 454
Score = 47.3 bits (97), Expect(3) = 2e-045
Identities = 19/24 (79%), Positives = 21/24 (87%)
Frame = -1 / -2
Query: 560 NSLTHGSSFTGISTNTRHTPANRP 489
+SLTHGSSFTGISTNTR TP +P
Sbjct: 551 HSLTHGSSFTGISTNTRETPP*QP 480
Score = 46.4 bits (95), Expect(3) = 1e-025
Identities = 20/33 (60%), Positives = 23/33 (69%)
Frame = -2 / -3
Query: 559 TA*HMAVPSQEFPRTQGTHQQIDQLHPKFPTNS 461
TA*HMAVPSQ FPRTQG H Q+ PT++
Sbjct: 550 TA*HMAVPSQAFPRTQGRHHPNSQIPSFQPTDT 452
Score = 46.0 bits (94), Expect(3) = 1e-025
Identities = 19/33 (57%), Positives = 21/33 (63%)
Frame = -2 / -1
Query: 103 THVHQRFSINSPWSGCVFRGGMPGKSRREVRSG 5
THVHQ FSIN PWSGC F +S+R R G
Sbjct: 99 THVHQHFSINFPWSGCGFAEERRHESQRGERFG 1
Score = 45.1 bits (92), Expect(3) = 3e-033
Identities = 15/18 (83%), Positives = 16/18 (88%)
Frame = +3 / +2
Query: 51 KTHPLQGELIEKRWWTWV 104
K HPLQG+LIEK WWTWV
Sbjct: 47 KPHPLQGKLIEKCWWTWV 100
Score = 42.8 bits (87), Expect(3) = 3e-033
Identities = 17/19 (89%), Positives = 18/19 (94%)
Frame = +1 / +1
Query: 502 GVCLVFVEIPVKELPCVRL 558
GV LVFVE+PVKELPCVRL
Sbjct: 493 GVSLVFVEMPVKELPCVRL 549
Score = 40.9 bits (83), Expect(2) = 4e-024
Identities = 14/15 (93%), Positives = 14/15 (93%)
Frame = +2 / +2
Query: 515 CSWKFL*RNCHVLGC 559
CSWK L*RNCHVLGC
Sbjct: 506 CSWKCL*RNCHVLGC 550
Score = 28.6 bits (56), Expect(3) = 1e-081
Identities = 8/11 (72%), Positives = 9/11 (81%)
Frame = +2 / +1
Query: 557 CYHAHFLNTMC 589
CYH HFLN +C
Sbjct: 550 CYHDHFLNVIC 582
Score = 22.6 bits (43), Expect(3) = 9e-078
Identities = 9/15 (60%), Positives = 11/15 (73%)
Frame = -1 / -3
Query: 590 NT*Y*ENGHGNSLTH 546
N *+*ENGH N+ H
Sbjct: 583 NR*H*ENGHDNTA*H 539
>gb|CD223298.1|CD223298 CCC1_27_E03.b1_A007 Callus culture/cell suspension Sorghum bicolor
cDNA clone CCC1_27_E03_A007 3', mRNA sequence
Length = 560
Score = 259 bits (559), Expect(3) = 4e-078
Identities = 112/134 (83%), Positives = 112/134 (83%)
Frame = -3 / -1
Query: 456 RN*STEQRRPF*RWKGG*GFKRSWSPPWPPGAHLXXXXXXXXXXXCW*LIGRRNSMMCGS 277
RN*STE RRPF*R KG *GFK WSPP P GAHL CW*LIGRRNSMMCGS
Sbjct: 422 RN*STE*RRPF*R*KGD*GFKHFWSPPLPAGAHLASNCAAAASCCCW*LIGRRNSMMCGS 243
Query: 276 GRWIA*CTHPRLCCTPIARHSFSERRRFLGRSLATSGGRTTCLYS*VSSKYFSEYWI*PM 97
GRWIA*C HPRLCCTPIARHSFSERRRFLGRSLATSGGRTTCLYS*VSSKYFSEYWI*PM
Sbjct: 242 GRWIA*CIHPRLCCTPIARHSFSERRRFLGRSLATSGGRTTCLYS*VSSKYFSEYWI*PM 63
Query: 96 STNASRSTPLGADA 55
STN SRST LGA A
Sbjct: 62 STNTSRSTSLGAGA 21
Score = 258 bits (557), Expect(3) = 1e-081
Identities = 108/136 (79%), Positives = 111/136 (81%)
Frame = +2 / +1
Query: 50 KNASAPRGVDREALVDMGQIQYSEKYFDDTYEYRHVVLPPEVAKLLPKNRLLSENEWRAI 229
K A APR VDRE LVDMGQIQYSEKYFDDTYEYRHVVLPPEVAKLLPKNRLLSENEWRAI
Sbjct: 16 KTAPAPREVDREVLVDMGQIQYSEKYFDDTYEYRHVVLPPEVAKLLPKNRLLSENEWRAI 195
Query: 230 GVQQSRGWVHYAIHRPEPHIMLFRRPINYXXXXXXXXXXXXXXXXPGGQGGDQERLKP*P 409
GVQQSRGW+HYAIHRPEPHIMLFRRPINY P G+GGDQ+ LKP*
Sbjct: 196 GVQQSRGWMHYAIHRPEPHIMLFRRPINYQQQQEAAAAQLLAK*APAGKGGDQKCLKP*S 375
Query: 410 PFHL*KGRRCSVDQFL 457
PFHL*KGRR SVD FL
Sbjct: 376 PFHL*KGRRYSVD*FL 423
Score = 124 bits (266), Expect(3) = 5e-045
Identities = 57/80 (71%), Positives = 61/80 (76%)
Frame = -2 / -3
Query: 457 EELINRTATPLLEMERGLGF*TFLVAALATXXXXXXXXXXXXXXXXLVVDRAAEQHDVRL 278
+ELINR ATPLLEM+RGLGF*TFLVA LA+ LVVDRAAEQHDVRL
Sbjct: 423 QELINRIATPLLEMKRGLGF*TFLVATLASRGSLGEQLRGSRLLLLLVVDRAAEQHDVRL 244
Query: 277 RAVDRVVHPPAALLHSDRAP 218
R VDRVVHPPAALLH+DRAP
Sbjct: 243 RTVDRVVHPPAALLHADRAP 184
Score = 93.6 bits (198), Expect(3) = 3e-033
Identities = 43/79 (54%), Positives = 46/79 (58%)
Frame = +3 / +2
Query: 219 GARSECSRAAGGXXXXXXXXXXXXXXXXXXXXXXXXXXXLPRRCWPSEPPVARAATRNV* 398
GARS CSRAAGG LPR C PSEP +AR ATRNV*
Sbjct: 185 GARSACSRAAGGCTTRSTVRSRTSCCSAARSTTSSSRRRLPRSCSPSEPLLARVATRNV* 364
Query: 399 NPNPLSISKRGVAVLLINS 455
NP+PL ISKRGVA+LLINS
Sbjct: 365 NPSPLFISKRGVAILLINS 421
Score = 91.8 bits (194), Expect(2) = 4e-024
Identities = 44/80 (55%), Positives = 45/80 (56%)
Frame = +1 / +3
Query: 220 ARDRSAAEPRVGALRDXXXXXXXXXXXXXDQLPXXXXXXXXXXXXQVSPRWPGRRPGTFK 399
ARDR AAEPRV ALRD DQLP QVSP W G RP FK
Sbjct: 186 ARDRRAAEPRVDALRDPPSGAAHHAVPPPDQLPAAAGGGCRAVARQVSPCWQGWRPEMFK 365
Query: 400 TLTPFPSLKGASLFC*SIPL 459
TL PF SLKGASLFC* IP+
Sbjct: 366 TLVPFSSLKGASLFC*LIPV 425
Score = 65.7 bits (137), Expect(3) = 7e-025
Identities = 25/34 (73%), Positives = 28/34 (82%)
Frame = -1 / -2
Query: 455 GIDQQNSDAPFRDGKGVRVLNVPGRRPGHRGLTW 354
GI+QQNSDAPFRD KG RVLN+ GR P +GLTW
Sbjct: 421 GINQQNSDAPFRDEKGTRVLNISGRHPCQQGLTW 320
Score = 57.4 bits (119), Expect(3) = 1e-081
Identities = 19/24 (79%), Positives = 22/24 (91%)
Frame = +3 / +3
Query: 489 WSICWCVPCVRGNSCEGTAMC*AV 560
W + WC+PCVRGN+CEGTAMC*AV
Sbjct: 450 WLLGWCLPCVRGNACEGTAMC*AV 521
Score = 49.6 bits (102), Expect(3) = 4e-078
Identities = 19/32 (59%), Positives = 22/32 (68%)
Frame = -3 / -3
Query: 558 QPNTWQFLHRNFHEHKAHTSKSTNYTRSFQLT 463
QPNTWQFLHR+FHEHK T+ + S Q T
Sbjct: 519 QPNTWQFLHRHFHEHKGDTTLTARSQVSNQQT 424
Score = 49.2 bits (101), Expect(3) = 5e-045
Identities = 18/20 (90%), Positives = 19/20 (95%)
Frame = -1 / -2
Query: 104 DPCPPTLLDQLPLERMRFSR 45
DPCPPTLLDQLPLER+RF R
Sbjct: 70 DPCPPTLLDQLPLERVRFCR 11
Score = 47.3 bits (97), Expect(3) = 5e-045
Identities = 19/24 (79%), Positives = 21/24 (87%)
Frame = -1 / -1
Query: 560 NSLTHGSSFTGISTNTRHTPANRP 489
+SLTHGSSFTGISTNTR TP +P
Sbjct: 521 HSLTHGSSFTGISTNTRETPP*QP 450
Score = 46.4 bits (95), Expect(3) = 7e-025
Identities = 20/33 (60%), Positives = 23/33 (69%)
Frame = -2 / -2
Query: 559 TA*HMAVPSQEFPRTQGTHQQIDQLHPKFPTNS 461
TA*HMAVPSQ FPRTQG H Q+ PT++
Sbjct: 520 TA*HMAVPSQAFPRTQGRHHPNSQIPSFQPTDT 422
Score = 45.1 bits (92), Expect(3) = 3e-033
Identities = 15/18 (83%), Positives = 16/18 (88%)
Frame = +3 / +2
Query: 51 KTHPLQGELIEKRWWTWV 104
K HPLQG+LIEK WWTWV
Sbjct: 17 KPHPLQGKLIEKCWWTWV 70
Score = 43.2 bits (88), Expect(3) = 7e-025
Identities = 15/18 (83%), Positives = 15/18 (83%)
Frame = -2 / -3
Query: 103 THVHQRFSINSPWSGCVF 50
THVHQ FSIN PWSGC F
Sbjct: 69 THVHQHFSINFPWSGCGF 16
Score = 42.8 bits (87), Expect(3) = 3e-033
Identities = 17/19 (89%), Positives = 18/19 (94%)
Frame = +1 / +1
Query: 502 GVCLVFVEIPVKELPCVRL 558
GV LVFVE+PVKELPCVRL
Sbjct: 463 GVSLVFVEMPVKELPCVRL 519
Score = 40.9 bits (83), Expect(2) = 4e-024
Identities = 14/15 (93%), Positives = 14/15 (93%)
Frame = +2 / +2
Query: 515 CSWKFL*RNCHVLGC 559
CSWK L*RNCHVLGC
Sbjct: 476 CSWKCL*RNCHVLGC 520
Score = 28.6 bits (56), Expect(3) = 1e-081
Identities = 8/11 (72%), Positives = 9/11 (81%)
Frame = +2 / +1
Query: 557 CYHAHFLNTMC 589
CYH HFLN +C
Sbjct: 520 CYHDHFLNVIC 552
Score = 24.0 bits (46), Expect(3) = 4e-078
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = -1 / -2
Query: 596 TVNT*Y*ENGHGNSLTH 546
T N *+*ENGH N+ H
Sbjct: 559 TSNR*H*ENGHDNTA*H 509
>gb|CD223408.1|CD223408 CCC1_27_E03.g1_A007 Callus culture/cell suspension Sorghum bicolor
cDNA clone CCC1_27_E03_A007 5', mRNA sequence
Length = 584
Score = 259 bits (559), Expect(3) = 3e-078
Identities = 112/134 (83%), Positives = 112/134 (83%)
Frame = -3 / -2
Query: 456 RN*STEQRRPF*RWKGG*GFKRSWSPPWPPGAHLXXXXXXXXXXXCW*LIGRRNSMMCGS 277
RN*STE RRPF*R KG *GFK WSPP P GAHL CW*LIGRRNSMMCGS
Sbjct: 424 RN*STE*RRPF*R*KGD*GFKHFWSPPLPAGAHLASNCAAAASCCCW*LIGRRNSMMCGS 245
Query: 276 GRWIA*CTHPRLCCTPIARHSFSERRRFLGRSLATSGGRTTCLYS*VSSKYFSEYWI*PM 97
GRWIA*C HPRLCCTPIARHSFSERRRFLGRSLATSGGRTTCLYS*VSSKYFSEYWI*PM
Sbjct: 244 GRWIA*CIHPRLCCTPIARHSFSERRRFLGRSLATSGGRTTCLYS*VSSKYFSEYWI*PM 65
Query: 96 STNASRSTPLGADA 55
STN SRST LGA A
Sbjct: 64 STNTSRSTSLGAGA 23
Score = 256 bits (553), Expect(3) = 5e-081
Identities = 107/136 (78%), Positives = 111/136 (81%)
Frame = +2 / +3
Query: 50 KNASAPRGVDREALVDMGQIQYSEKYFDDTYEYRHVVLPPEVAKLLPKNRLLSENEWRAI 229
+ A APR VDRE LVDMGQIQYSEKYFDDTYEYRHVVLPPEVAKLLPKNRLLSENEWRAI
Sbjct: 18 QTAPAPREVDREVLVDMGQIQYSEKYFDDTYEYRHVVLPPEVAKLLPKNRLLSENEWRAI 197
Query: 230 GVQQSRGWVHYAIHRPEPHIMLFRRPINYXXXXXXXXXXXXXXXXPGGQGGDQERLKP*P 409
GVQQSRGW+HYAIHRPEPHIMLFRRPINY P G+GGDQ+ LKP*
Sbjct: 198 GVQQSRGWMHYAIHRPEPHIMLFRRPINYQQQQEAAAAQLLAK*APAGKGGDQKCLKP*S 377
Query: 410 PFHL*KGRRCSVDQFL 457
PFHL*KGRR SVD FL
Sbjct: 378 PFHL*KGRRYSVD*FL 425
Score = 124 bits (266), Expect(3) = 1e-044
Identities = 57/80 (71%), Positives = 61/80 (76%)
Frame = -2 / -1
Query: 457 EELINRTATPLLEMERGLGF*TFLVAALATXXXXXXXXXXXXXXXXLVVDRAAEQHDVRL 278
+ELINR ATPLLEM+RGLGF*TFLVA LA+ LVVDRAAEQHDVRL
Sbjct: 425 QELINRIATPLLEMKRGLGF*TFLVATLASRGSLGEQLRGSRLLLLLVVDRAAEQHDVRL 246
Query: 277 RAVDRVVHPPAALLHSDRAP 218
R VDRVVHPPAALLH+DRAP
Sbjct: 245 RTVDRVVHPPAALLHADRAP 186
Score = 93.6 bits (198), Expect(3) = 1e-034
Identities = 43/79 (54%), Positives = 46/79 (58%)
Frame = +3 / +1
Query: 219 GARSECSRAAGGXXXXXXXXXXXXXXXXXXXXXXXXXXXLPRRCWPSEPPVARAATRNV* 398
GARS CSRAAGG LPR C PSEP +AR ATRNV*
Sbjct: 187 GARSACSRAAGGCTTRSTVRSRTSCCSAARSTTSSSRRRLPRSCSPSEPLLARVATRNV* 366
Query: 399 NPNPLSISKRGVAVLLINS 455
NP+PL ISKRGVA+LLINS
Sbjct: 367 NPSPLFISKRGVAILLINS 423
Score = 91.8 bits (194), Expect(2) = 2e-024
Identities = 44/80 (55%), Positives = 45/80 (56%)
Frame = +1 / +2
Query: 220 ARDRSAAEPRVGALRDXXXXXXXXXXXXXDQLPXXXXXXXXXXXXQVSPRWPGRRPGTFK 399
ARDR AAEPRV ALRD DQLP QVSP W G RP FK
Sbjct: 188 ARDRRAAEPRVDALRDPPSGAAHHAVPPPDQLPAAAGGGCRAVARQVSPCWQGWRPEMFK 367
Query: 400 TLTPFPSLKGASLFC*SIPL 459
TL PF SLKGASLFC* IP+
Sbjct: 368 TLVPFSSLKGASLFC*LIPV 427
Score = 65.7 bits (137), Expect(3) = 2e-024
Identities = 25/34 (73%), Positives = 28/34 (82%)
Frame = -1 / -3
Query: 455 GIDQQNSDAPFRDGKGVRVLNVPGRRPGHRGLTW 354
GI+QQNSDAPFRD KG RVLN+ GR P +GLTW
Sbjct: 423 GINQQNSDAPFRDEKGTRVLNISGRHPCQQGLTW 322
Score = 57.4 bits (119), Expect(3) = 5e-081
Identities = 19/24 (79%), Positives = 22/24 (91%)
Frame = +3 / +2
Query: 489 WSICWCVPCVRGNSCEGTAMC*AV 560
W + WC+PCVRGN+CEGTAMC*AV
Sbjct: 452 WLLGWCLPCVRGNACEGTAMC*AV 523
Score = 51.5 bits (106), Expect(3) = 3e-078
Identities = 20/32 (62%), Positives = 23/32 (71%)
Frame = -3 / -1
Query: 558 QPNTWQFLHRNFHEHKAHTSKSTNYTRSFQLT 463
QPNTWQFLHR+FHEHKA T+ + S Q T
Sbjct: 521 QPNTWQFLHRHFHEHKADTTLTARSQVSNQQT 426
Score = 47.8 bits (98), Expect(3) = 1e-044
Identities = 17/19 (89%), Positives = 19/19 (100%)
Frame = -1 / -3
Query: 104 DPCPPTLLDQLPLERMRFS 48
DPCPPTLLDQLPLER+RF+
Sbjct: 72 DPCPPTLLDQLPLERVRFA 16
Score = 47.3 bits (97), Expect(3) = 1e-034
Identities = 18/19 (94%), Positives = 19/19 (100%)
Frame = +1 / +3
Query: 502 GVCLVFVEIPVKELPCVRL 558
GVCLVFVE+PVKELPCVRL
Sbjct: 465 GVCLVFVEMPVKELPCVRL 521
Score = 47.3 bits (97), Expect(3) = 1e-044
Identities = 19/24 (79%), Positives = 21/24 (87%)
Frame = -1 / -2
Query: 560 NSLTHGSSFTGISTNTRHTPANRP 489
+SLTHGSSFTGISTNTR TP +P
Sbjct: 523 HSLTHGSSFTGISTNTRQTPP*QP 452
Score = 46.4 bits (95), Expect(3) = 2e-024
Identities = 20/33 (60%), Positives = 23/33 (69%)
Frame = -2 / -3
Query: 559 TA*HMAVPSQEFPRTQGTHQQIDQLHPKFPTNS 461
TA*HMAVPSQ FPRTQG H Q+ PT++
Sbjct: 522 TA*HMAVPSQAFPRTQGRHHPNSQIPSFQPTDT 424
Score = 45.1 bits (92), Expect(3) = 1e-034
Identities = 15/18 (83%), Positives = 16/18 (88%)
Frame = +3 / +1
Query: 51 KTHPLQGELIEKRWWTWV 104
K HPLQG+LIEK WWTWV
Sbjct: 19 KPHPLQGKLIEKCWWTWV 72
Score = 41.8 bits (85), Expect(3) = 2e-024
Identities = 14/16 (87%), Positives = 14/16 (87%)
Frame = -2 / -1
Query: 103 THVHQRFSINSPWSGC 56
THVHQ FSIN PWSGC
Sbjct: 71 THVHQHFSINFPWSGC 24
Score = 41.8 bits (85), Expect(2) = 2e-024
Identities = 16/32 (50%), Positives = 16/32 (50%)
Frame = +2 / +1
Query: 464 VSWKXXXXXXXXXXXXXCSWKFL*RNCHVLGC 559
V W CSWK L*RNCHVLGC
Sbjct: 427 VCWLETWDLAVRVVSALCSWKCL*RNCHVLGC 522
Score = 28.6 bits (56), Expect(3) = 5e-081
Identities = 8/11 (72%), Positives = 9/11 (81%)
Frame = +2 / +3
Query: 557 CYHAHFLNTMC 589
CYH HFLN +C
Sbjct: 522 CYHDHFLNVIC 554
Score = 22.6 bits (43), Expect(3) = 3e-078
Identities = 9/15 (60%), Positives = 11/15 (73%)
Frame = -1 / -3
Query: 590 NT*Y*ENGHGNSLTH 546
N *+*ENGH N+ H
Sbjct: 555 NR*H*ENGHDNTA*H 511
>gb|CF485791.1|CF485791 POL1_33_E06.g1_A002 Pollen Sorghum bicolor cDNA clone
POL1_33_E06_A002 5', mRNA sequence
Length = 575
Score = 254 bits (549), Expect(3) = 6e-077
Identities = 111/134 (82%), Positives = 111/134 (82%)
Frame = -3 / +3
Query: 456 RN*STEQRRPF*RWKGG*GFKRSWSPPWPPGAHLXXXXXXXXXXXCW*LIGRRNSMMCGS 277
RN*STE RRPF*R KG *GFK WSPP P GAHL CW*LIGRRNSMMCGS
Sbjct: 159 RN*STE*RRPF*R*KGD*GFKHFWSPPLPAGAHLASNCAAAASCCCW*LIGRRNSMMCGS 338
Query: 276 GRWIA*CTHPRLCCTPIARHSFSERRRFLGRSLATSGGRTTCLYS*VSSKYFSEYWI*PM 97
GRWIA*C HPRLCCTPIARHSFSERRRFLGRSLATSGGRTTCLYS*VSSKYFSE WI*PM
Sbjct: 339 GRWIA*CIHPRLCCTPIARHSFSERRRFLGRSLATSGGRTTCLYS*VSSKYFSEDWI*PM 518
Query: 96 STNASRSTPLGADA 55
STN SRST LGA A
Sbjct: 519 STNTSRSTSLGAGA 560
Score = 253 bits (546), Expect(3) = 4e-080
Identities = 107/136 (78%), Positives = 110/136 (80%)
Frame = +2 / -2
Query: 50 KNASAPRGVDREALVDMGQIQYSEKYFDDTYEYRHVVLPPEVAKLLPKNRLLSENEWRAI 229
K A APR VDRE LVDMGQIQ SEKYFDDTYEYRHVVLPPEVAKLLPKNRLLSENEWRAI
Sbjct: 565 KPAPAPREVDREVLVDMGQIQSSEKYFDDTYEYRHVVLPPEVAKLLPKNRLLSENEWRAI 386
Query: 230 GVQQSRGWVHYAIHRPEPHIMLFRRPINYXXXXXXXXXXXXXXXXPGGQGGDQERLKP*P 409
GVQQSRGW+HYAIHRPEPHIMLFRRPINY P G+GGDQ+ LKP*
Sbjct: 385 GVQQSRGWMHYAIHRPEPHIMLFRRPINYQQQQEAAAAQLLAK*APAGKGGDQKCLKP*S 206
Query: 410 PFHL*KGRRCSVDQFL 457
PFHL*KGRR SVD FL
Sbjct: 205 PFHL*KGRRYSVD*FL 158
Score = 124 bits (266), Expect(3) = 4e-044
Identities = 57/80 (71%), Positives = 61/80 (76%)
Frame = -2 / +2
Query: 457 EELINRTATPLLEMERGLGF*TFLVAALATXXXXXXXXXXXXXXXXLVVDRAAEQHDVRL 278
+ELINR ATPLLEM+RGLGF*TFLVA LA+ LVVDRAAEQHDVRL
Sbjct: 158 QELINRIATPLLEMKRGLGF*TFLVATLASRGSLGEQLRGSRLLLLLVVDRAAEQHDVRL 337
Query: 277 RAVDRVVHPPAALLHSDRAP 218
R VDRVVHPPAALLH+DRAP
Sbjct: 338 RTVDRVVHPPAALLHADRAP 397
Score = 93.6 bits (198), Expect(3) = 4e-034
Identities = 43/79 (54%), Positives = 46/79 (58%)
Frame = +3 / -3
Query: 219 GARSECSRAAGGXXXXXXXXXXXXXXXXXXXXXXXXXXXLPRRCWPSEPPVARAATRNV* 398
GARS CSRAAGG LPR C PSEP +AR ATRNV*
Sbjct: 396 GARSACSRAAGGCTTRSTVRSRTSCCSAARSTTSSSRRRLPRSCSPSEPLLARVATRNV* 217
Query: 399 NPNPLSISKRGVAVLLINS 455
NP+PL ISKRGVA+LLINS
Sbjct: 216 NPSPLFISKRGVAILLINS 160
Score = 91.8 bits (194), Expect(2) = 2e-024
Identities = 44/80 (55%), Positives = 45/80 (56%)
Frame = +1 / -1
Query: 220 ARDRSAAEPRVGALRDXXXXXXXXXXXXXDQLPXXXXXXXXXXXXQVSPRWPGRRPGTFK 399
ARDR AAEPRV ALRD DQLP QVSP W G RP FK
Sbjct: 395 ARDRRAAEPRVDALRDPPSGAAHHAVPPPDQLPAAAGGGCRAVARQVSPCWQGWRPEMFK 216
Query: 400 TLTPFPSLKGASLFC*SIPL 459
TL PF SLKGASLFC* IP+
Sbjct: 215 TLVPFSSLKGASLFC*LIPV 156
Score = 65.7 bits (137), Expect(3) = 7e-025
Identities = 25/34 (73%), Positives = 28/34 (82%)
Frame = -1 / +1
Query: 455 GIDQQNSDAPFRDGKGVRVLNVPGRRPGHRGLTW 354
GI+QQNSDAPFRD KG RVLN+ GR P +GLTW
Sbjct: 160 GINQQNSDAPFRDEKGTRVLNISGRHPCQQGLTW 261
Score = 57.4 bits (119), Expect(3) = 4e-080
Identities = 19/24 (79%), Positives = 22/24 (91%)
Frame = +3 / -1
Query: 489 WSICWCVPCVRGNSCEGTAMC*AV 560
W + WC+PCVRGN+CEGTAMC*AV
Sbjct: 131 WLLGWCLPCVRGNACEGTAMC*AV 60
Score = 51.5 bits (106), Expect(3) = 6e-077
Identities = 20/32 (62%), Positives = 23/32 (71%)
Frame = -3 / +2
Query: 558 QPNTWQFLHRNFHEHKAHTSKSTNYTRSFQLT 463
QPNTWQFLHR+FHEHKA T+ + S Q T
Sbjct: 62 QPNTWQFLHRHFHEHKADTTLTARSQVSNQQT 157
Score = 47.3 bits (97), Expect(3) = 4e-034
Identities = 18/19 (94%), Positives = 19/19 (100%)
Frame = +1 / -2
Query: 502 GVCLVFVEIPVKELPCVRL 558
GVCLVFVE+PVKELPCVRL
Sbjct: 118 GVCLVFVEMPVKELPCVRL 62
Score = 47.3 bits (97), Expect(3) = 4e-044
Identities = 19/24 (79%), Positives = 21/24 (87%)
Frame = -1 / +3
Query: 560 NSLTHGSSFTGISTNTRHTPANRP 489
+SLTHGSSFTGISTNTR TP +P
Sbjct: 60 HSLTHGSSFTGISTNTRQTPP*QP 131
Score = 46.4 bits (95), Expect(3) = 7e-025
Identities = 20/33 (60%), Positives = 23/33 (69%)
Frame = -2 / +1
Query: 559 TA*HMAVPSQEFPRTQGTHQQIDQLHPKFPTNS 461
TA*HMAVPSQ FPRTQG H Q+ PT++
Sbjct: 61 TA*HMAVPSQAFPRTQGRHHPNSQIPSFQPTDT 159
Score = 46.0 bits (94), Expect(3) = 4e-044
Identities = 17/20 (85%), Positives = 18/20 (90%)
Frame = -1 / +1
Query: 104 DPCPPTLLDQLPLERMRFSR 45
DPCPPTLLDQLPLER+R R
Sbjct: 511 DPCPPTLLDQLPLERVRVCR 570
Score = 43.2 bits (88), Expect(3) = 7e-025
Identities = 15/18 (83%), Positives = 15/18 (83%)
Frame = -2 / +2
Query: 103 THVHQRFSINSPWSGCVF 50
THVHQ FSIN PWSGC F
Sbjct: 512 THVHQHFSINFPWSGCGF 565
Score = 43.2 bits (88), Expect(3) = 4e-034
Identities = 14/16 (87%), Positives = 15/16 (93%)
Frame = +3 / -3
Query: 57 HPLQGELIEKRWWTWV 104
HPLQG+LIEK WWTWV
Sbjct: 558 HPLQGKLIEKCWWTWV 511
Score = 41.8 bits (85), Expect(2) = 2e-024
Identities = 16/32 (50%), Positives = 16/32 (50%)
Frame = +2 / -3
Query: 464 VSWKXXXXXXXXXXXXXCSWKFL*RNCHVLGC 559
V W CSWK L*RNCHVLGC
Sbjct: 156 VCWLETWDLAVRVVSALCSWKCL*RNCHVLGC 61
Score = 28.6 bits (56), Expect(3) = 4e-080
Identities = 8/11 (72%), Positives = 9/11 (81%)
Frame = +2 / -2
Query: 557 CYHAHFLNTMC 589
CYH HFLN +C
Sbjct: 61 CYHDHFLNVIC 29
Score = 22.6 bits (43), Expect(3) = 6e-077
Identities = 9/15 (60%), Positives = 11/15 (73%)
Frame = -1 / +1
Query: 590 NT*Y*ENGHGNSLTH 546
N *+*ENGH N+ H
Sbjct: 28 NR*H*ENGHDNTA*H 72
>gb|CF485720.1|CF485720 POL1_33_E06.b1_A002 Pollen Sorghum bicolor cDNA clone
POL1_33_E06_A002 3', mRNA sequence
Length = 607
Score = 116 bits (247), Expect = 6e-025
Identities = 48/52 (92%), Positives = 48/52 (92%)
Frame = +2 / -3
Query: 50 KNASAPRGVDREALVDMGQIQYSEKYFDDTYEYRHVVLPPEVAKLLPKNRLL 205
K A APR VDRE LVDMGQIQYSEKYFDDTYEYRHVVLPPEVAKLLPKNRLL
Sbjct: 158 KTAPAPREVDREVLVDMGQIQYSEKYFDDTYEYRHVVLPPEVAKLLPKNRLL 3
Score = 111 bits (237), Expect = 1e-023
Identities = 48/51 (94%), Positives = 48/51 (94%)
Frame = -3 / +1
Query: 207 ERRRFLGRSLATSGGRTTCLYS*VSSKYFSEYWI*PMSTNASRSTPLGADA 55
ERRRFLGRSLATSGGRTTCLYS*VSSKYFSEYWI*PMSTN SRST LGA A
Sbjct: 1 ERRRFLGRSLATSGGRTTCLYS*VSSKYFSEYWI*PMSTNTSRSTSLGAGA 153
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 871,448,636
Number of Sequences: 832831
Number of extensions: 12489033
Number of successful extensions: 104440
Number of sequences better than 1.0e-020: 5
length of database: 163,786,556
effective HSP length: 54
effective length of database: 118,813,682
effective search space used: 24356804810
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)