TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2594211.2.1
(668 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG355810.1|BG355810 EM1_17_A05.g1_A002 Embryo 1 (EM1) So... 128 6e-057
gb|BG556323.1|BG556323 EM1_68_E09.g1_A002 Embryo 1 (EM1) So... 128 9e-057
gb|CW426483.1|CW426483 fsbb001f137o10k0 Sorghum methylation... 128 2e-056
gb|CL177979.1|CL177979 104_385_10894229_148_31913_293 Sorgh... 128 2e-056
gb|CL177978.1|CL177978 104_385_10894229_116_31914_293 Sorgh... 128 3e-045
gb|CW325048.1|CW325048 104_819_11477484_116_35915_034 Sorgh... 128 3e-045
gb|BG322950.1|BG322950 EM1_13_B01.g1_A002 Embryo 1 (EM1) So... 86 6e-045
gb|BG556478.1|BG556478 EM1_37_F06.g1_A002 Embryo 1 (EM1) So... 93 2e-035
gb|CW325049.1|CW325049 104_819_11477484_148_35911_034 Sorgh... 54 6e-024
gb|CN151243.1|CN151243 WOUND1_74_G01.b1_A002 Wounded leaves... 101 9e-021
>gb|BG355810.1|BG355810 EM1_17_A05.g1_A002 Embryo 1 (EM1) Sorghum bicolor cDNA, mRNA
sequence
Length = 611
Score = 128 bits (275), Expect(6) = 6e-057
Identities = 51/54 (94%), Positives = 53/54 (98%)
Frame = -3 / +3
Query: 648 HWGQNWQSNTLLDGQALSFRVTTSDGRSVVSSNAAPRGWGFGQTFSGAQFN*TP 487
+WGQNWQSNTLLDGQALSFRVTTSDGRSVVS+NAAPRGW FGQTFSGAQFN*TP
Sbjct: 42 NWGQNWQSNTLLDGQALSFRVTTSDGRSVVSNNAAPRGWAFGQTFSGAQFN*TP 203
Score = 127 bits (272), Expect(6) = 2e-052
Identities = 50/55 (90%), Positives = 51/55 (92%)
Frame = +2 / -2
Query: 482 MEGVQLNWAPLKVWPKPQPRGAALLETTERPSLVVTRKERACPSRSVLLCQFWPQ 646
+ GVQLNWAPLKVWPK QPRGAALLETTERPSLVVTR E ACPSRSVLLCQFWPQ
Sbjct: 208 VSGVQLNWAPLKVWPKAQPRGAALLETTERPSLVVTRNESACPSRSVLLCQFWPQ 44
Score = 53.8 bits (111), Expect(6) = 6e-057
Identities = 19/27 (70%), Positives = 21/27 (77%)
Frame = -2 / +1
Query: 400 PSLGACREAPWFYEWCISLWIWGITSS 320
P+ REAPWFYEWCIS W+ GITSS
Sbjct: 298 PNWSLPREAPWFYEWCISHWLLGITSS 378
Score = 47.3 bits (97), Expect(5) = 1e-023
Identities = 26/47 (55%), Positives = 29/47 (61%)
Frame = -1 / +3
Query: 458 RRRRGATSSSFPLVVVESSTLTRSLPRGAMVL*MVHLALDLGNYQ*F 318
RR G+TSSS +V RGAMVL*+VHLAL GNYQ*F
Sbjct: 240 RRSGGSTSSSSVVVESSVHPQLELAERGAMVL*VVHLALAPGNYQ*F 380
Score = 47.3 bits (97), Expect(6) = 2e-052
Identities = 17/21 (80%), Positives = 18/21 (85%)
Frame = +1 / -2
Query: 319 NYW*FPRSKARCTIHKTMAPL 381
NYW*FP + ARCT HKTMAPL
Sbjct: 379 NYW*FPGANARCTTHKTMAPL 317
Score = 46.9 bits (96), Expect(6) = 6e-057
Identities = 18/23 (78%), Positives = 21/23 (91%)
Frame = -2 / +3
Query: 223 KAGLQINGGRPPFITRQLSICGV 155
KAGL+INGGRPP ITRQLS+ G+
Sbjct: 474 KAGLEINGGRPPCITRQLSVLGL 542
Score = 45.1 bits (92), Expect(5) = 1e-023
Identities = 18/21 (85%), Positives = 18/21 (85%)
Frame = -2 / +2
Query: 544 PARLGLRPDLQRGPVQLNTLH 482
P RLGLRPDLQR PVQLNT H
Sbjct: 146 PPRLGLRPDLQRRPVQLNTRH 208
Score = 41.8 bits (85), Expect(6) = 2e-052
Identities = 22/46 (47%), Positives = 28/46 (60%)
Frame = +2 / -1
Query: 86 GFITTNTLG*HAKHLIRKRPSP*DPTNA*LAGDKRRPTSVNLQASL 223
G + + + + K + KR + NA*LAGD RRP SVNLQASL
Sbjct: 611 GLLQPHLITHNHKTSLLKRGHHHEAQNA*LAGDTRRPASVNLQASL 474
Score = 36.8 bits (74), Expect(5) = 1e-023
Identities = 14/14 (100%), Positives = 14/14 (100%)
Frame = -2 / +2
Query: 646 LGPELAEQHAPGRA 605
LGPELAEQHAPGRA
Sbjct: 44 LGPELAEQHAPGRA 85
Score = 32.7 bits (65), Expect(6) = 6e-057
Identities = 12/18 (66%), Positives = 14/18 (77%)
Frame = -2 / +2
Query: 337 WGITSSLGLVLARVLSCW 284
W + LGLVLA+VLSCW
Sbjct: 362 WELPVVLGLVLAQVLSCW 415
Score = 31.8 bits (63), Expect(5) = 1e-023
Identities = 13/16 (81%), Positives = 13/16 (81%)
Frame = -1 / +2
Query: 215 LAD*RR*AAFYHPPIK 168
L D*RR AA YHPPIK
Sbjct: 482 LGD*RRQAALYHPPIK 529
Score = 29.9 bits (59), Expect(6) = 2e-052
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = +2 / -3
Query: 398 GWTTLPRPTENYYLWHRVD 454
G TTLPRPT +Y H VD
Sbjct: 294 GPTTLPRPTNYWYYHHCVD 238
Score = 29.5 bits (58), Expect(6) = 6e-057
Identities = 9/15 (60%), Positives = 10/15 (66%)
Frame = -2 / +2
Query: 271 WCGSVLLGCFGIGSG 227
WC LGCFG+G G
Sbjct: 419 WCVLAFLGCFGLGIG 463
Score = 29.5 bits (58), Expect(6) = 2e-052
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = +3 / -3
Query: 285 QHDKTRANTRPK 320
QHDKT ANTRPK
Sbjct: 414 QHDKTCANTRPK 379
Score = 21.7 bits (41), Expect(6) = 2e-052
Identities = 7/9 (77%), Positives = 7/9 (77%)
Frame = +2 / -3
Query: 641 PQCATGPAT 667
P CAT PAT
Sbjct: 48 PSCATAPAT 22
Score = 21.7 bits (41), Expect(6) = 6e-057
Identities = 7/8 (87%), Positives = 7/8 (87%)
Frame = -3 / +2
Query: 159 GSHGDGLF 136
G HGDGLF
Sbjct: 536 GPHGDGLF 559
Score = 21.7 bits (41), Expect(5) = 1e-023
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = -1 / +1
Query: 338 LGNYQ*FRPSISAG 297
LG FRPSISAG
Sbjct: 361 LGITSSFRPSISAG 402
>gb|BG556323.1|BG556323 EM1_68_E09.g1_A002 Embryo 1 (EM1) Sorghum bicolor cDNA, mRNA
sequence
Length = 653
Score = 128 bits (275), Expect(6) = 9e-057
Identities = 51/54 (94%), Positives = 53/54 (98%)
Frame = -3 / +3
Query: 648 HWGQNWQSNTLLDGQALSFRVTTSDGRSVVSSNAAPRGWGFGQTFSGAQFN*TP 487
+WGQNWQSNTLLDGQALSFRVTTSDGRSVVS+NAAPRGW FGQTFSGAQFN*TP
Sbjct: 78 NWGQNWQSNTLLDGQALSFRVTTSDGRSVVSNNAAPRGWAFGQTFSGAQFN*TP 239
Score = 127 bits (272), Expect(6) = 2e-052
Identities = 50/55 (90%), Positives = 51/55 (92%)
Frame = +2 / -2
Query: 482 MEGVQLNWAPLKVWPKPQPRGAALLETTERPSLVVTRKERACPSRSVLLCQFWPQ 646
+ GVQLNWAPLKVWPK QPRGAALLETTERPSLVVTR E ACPSRSVLLCQFWPQ
Sbjct: 244 VSGVQLNWAPLKVWPKAQPRGAALLETTERPSLVVTRNESACPSRSVLLCQFWPQ 80
Score = 53.8 bits (111), Expect(6) = 9e-057
Identities = 19/27 (70%), Positives = 21/27 (77%)
Frame = -2 / +1
Query: 400 PSLGACREAPWFYEWCISLWIWGITSS 320
P+ REAPWFYEWCIS W+ GITSS
Sbjct: 334 PNWSLPREAPWFYEWCISHWLLGITSS 414
Score = 47.3 bits (97), Expect(5) = 2e-023
Identities = 26/47 (55%), Positives = 29/47 (61%)
Frame = -1 / +3
Query: 458 RRRRGATSSSFPLVVVESSTLTRSLPRGAMVL*MVHLALDLGNYQ*F 318
RR G+TSSS +V RGAMVL*+VHLAL GNYQ*F
Sbjct: 276 RRSGGSTSSSSVVVESSVHPQLELAERGAMVL*VVHLALAPGNYQ*F 416
Score = 47.3 bits (97), Expect(6) = 2e-052
Identities = 17/21 (80%), Positives = 18/21 (85%)
Frame = +1 / -2
Query: 319 NYW*FPRSKARCTIHKTMAPL 381
NYW*FP + ARCT HKTMAPL
Sbjct: 415 NYW*FPGANARCTTHKTMAPL 353
Score = 46.9 bits (96), Expect(6) = 9e-057
Identities = 18/23 (78%), Positives = 21/23 (91%)
Frame = -2 / +3
Query: 223 KAGLQINGGRPPFITRQLSICGV 155
KAGL+INGGRPP ITRQLS+ G+
Sbjct: 510 KAGLEINGGRPPCITRQLSVLGL 578
Score = 45.1 bits (92), Expect(5) = 2e-023
Identities = 18/21 (85%), Positives = 18/21 (85%)
Frame = -2 / +2
Query: 544 PARLGLRPDLQRGPVQLNTLH 482
P RLGLRPDLQR PVQLNT H
Sbjct: 182 PPRLGLRPDLQRRPVQLNTRH 244
Score = 42.8 bits (87), Expect(6) = 2e-052
Identities = 23/48 (47%), Positives = 29/48 (60%)
Frame = +2 / -1
Query: 80 LIGFITTNTLG*HAKHLIRKRPSP*DPTNA*LAGDKRRPTSVNLQASL 223
+IG + + + K + KR + NA*LAGD RRP SVNLQASL
Sbjct: 653 VIGSLQPHLITHKPKTSLLKRGHHHEAQNA*LAGDTRRPASVNLQASL 510
Score = 36.8 bits (74), Expect(5) = 2e-023
Identities = 14/14 (100%), Positives = 14/14 (100%)
Frame = -2 / +2
Query: 646 LGPELAEQHAPGRA 605
LGPELAEQHAPGRA
Sbjct: 80 LGPELAEQHAPGRA 121
Score = 32.7 bits (65), Expect(6) = 9e-057
Identities = 12/18 (66%), Positives = 14/18 (77%)
Frame = -2 / +2
Query: 337 WGITSSLGLVLARVLSCW 284
W + LGLVLA+VLSCW
Sbjct: 398 WELPVVLGLVLAQVLSCW 451
Score = 31.8 bits (63), Expect(5) = 2e-023
Identities = 13/16 (81%), Positives = 13/16 (81%)
Frame = -1 / +2
Query: 215 LAD*RR*AAFYHPPIK 168
L D*RR AA YHPPIK
Sbjct: 518 LGD*RRQAALYHPPIK 565
Score = 29.9 bits (59), Expect(6) = 2e-052
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = +2 / -3
Query: 398 GWTTLPRPTENYYLWHRVD 454
G TTLPRPT +Y H VD
Sbjct: 330 GPTTLPRPTNYWYYHHCVD 274
Score = 29.5 bits (58), Expect(6) = 9e-057
Identities = 9/15 (60%), Positives = 10/15 (66%)
Frame = -2 / +2
Query: 271 WCGSVLLGCFGIGSG 227
WC LGCFG+G G
Sbjct: 455 WCVLAFLGCFGLGIG 499
Score = 29.5 bits (58), Expect(6) = 2e-052
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = +3 / -3
Query: 285 QHDKTRANTRPK 320
QHDKT ANTRPK
Sbjct: 450 QHDKTCANTRPK 415
Score = 21.7 bits (41), Expect(6) = 9e-057
Identities = 7/8 (87%), Positives = 7/8 (87%)
Frame = -3 / +2
Query: 159 GSHGDGLF 136
G HGDGLF
Sbjct: 572 GPHGDGLF 595
Score = 21.7 bits (41), Expect(6) = 2e-052
Identities = 7/9 (77%), Positives = 7/9 (77%)
Frame = +2 / -3
Query: 641 PQCATGPAT 667
P CAT PAT
Sbjct: 84 PSCATAPAT 58
Score = 21.7 bits (41), Expect(5) = 2e-023
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = -1 / +1
Query: 338 LGNYQ*FRPSISAG 297
LG FRPSISAG
Sbjct: 397 LGITSSFRPSISAG 438
>gb|CW426483.1|CW426483 fsbb001f137o10k0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f137o10, DNA
sequence
Length = 727
Score = 128 bits (275), Expect(6) = 2e-056
Identities = 51/54 (94%), Positives = 53/54 (98%)
Frame = -3 / +3
Query: 648 HWGQNWQSNTLLDGQALSFRVTTSDGRSVVSSNAAPRGWGFGQTFSGAQFN*TP 487
+WGQNWQSNTLLDGQALSFRVTTSDGRSVVS+NAAPRGW FGQTFSGAQFN*TP
Sbjct: 189 NWGQNWQSNTLLDGQALSFRVTTSDGRSVVSNNAAPRGWAFGQTFSGAQFN*TP 350
Score = 127 bits (272), Expect(6) = 7e-052
Identities = 50/55 (90%), Positives = 51/55 (92%)
Frame = +2 / -1
Query: 482 MEGVQLNWAPLKVWPKPQPRGAALLETTERPSLVVTRKERACPSRSVLLCQFWPQ 646
+ GVQLNWAPLKVWPK QPRGAALLETTERPSLVVTR E ACPSRSVLLCQFWPQ
Sbjct: 355 VSGVQLNWAPLKVWPKAQPRGAALLETTERPSLVVTRNESACPSRSVLLCQFWPQ 191
Score = 53.8 bits (111), Expect(6) = 2e-056
Identities = 19/27 (70%), Positives = 21/27 (77%)
Frame = -2 / +1
Query: 400 PSLGACREAPWFYEWCISLWIWGITSS 320
P+ REAPWFYEWCIS W+ GITSS
Sbjct: 445 PNWSLPREAPWFYEWCISHWLLGITSS 525
Score = 47.3 bits (97), Expect(5) = 3e-023
Identities = 26/47 (55%), Positives = 29/47 (61%)
Frame = -1 / +3
Query: 458 RRRRGATSSSFPLVVVESSTLTRSLPRGAMVL*MVHLALDLGNYQ*F 318
RR G+TSSS +V RGAMVL*+VHLAL GNYQ*F
Sbjct: 387 RRSGGSTSSSSVVVESSVHPQLELAERGAMVL*VVHLALAPGNYQ*F 527
Score = 47.3 bits (97), Expect(6) = 7e-052
Identities = 17/21 (80%), Positives = 18/21 (85%)
Frame = +1 / -1
Query: 319 NYW*FPRSKARCTIHKTMAPL 381
NYW*FP + ARCT HKTMAPL
Sbjct: 526 NYW*FPGANARCTTHKTMAPL 464
Score = 46.9 bits (96), Expect(6) = 2e-056
Identities = 18/23 (78%), Positives = 21/23 (91%)
Frame = -2 / +3
Query: 223 KAGLQINGGRPPFITRQLSICGV 155
KAGL+INGGRPP ITRQLS+ G+
Sbjct: 621 KAGLEINGGRPPCITRQLSVLGL 689
Score = 45.1 bits (92), Expect(5) = 3e-023
Identities = 18/21 (85%), Positives = 18/21 (85%)
Frame = -2 / +2
Query: 544 PARLGLRPDLQRGPVQLNTLH 482
P RLGLRPDLQR PVQLNT H
Sbjct: 293 PPRLGLRPDLQRRPVQLNTRH 355
Score = 41.4 bits (84), Expect(6) = 7e-052
Identities = 21/34 (61%), Positives = 23/34 (67%)
Frame = +2 / -3
Query: 122 KHLIRKRPSP*DPTNA*LAGDKRRPTSVNLQASL 223
K + KR + NA*LAGD RRP SVNLQASL
Sbjct: 722 KTSLLKRGHHHEAQNA*LAGDTRRPASVNLQASL 621
Score = 36.8 bits (74), Expect(5) = 3e-023
Identities = 14/14 (100%), Positives = 14/14 (100%)
Frame = -2 / +2
Query: 646 LGPELAEQHAPGRA 605
LGPELAEQHAPGRA
Sbjct: 191 LGPELAEQHAPGRA 232
Score = 32.7 bits (65), Expect(6) = 2e-056
Identities = 12/18 (66%), Positives = 14/18 (77%)
Frame = -2 / +2
Query: 337 WGITSSLGLVLARVLSCW 284
W + LGLVLA+VLSCW
Sbjct: 509 WELPVVLGLVLAQVLSCW 562
Score = 31.8 bits (63), Expect(5) = 3e-023
Identities = 13/16 (81%), Positives = 13/16 (81%)
Frame = -1 / +2
Query: 215 LAD*RR*AAFYHPPIK 168
L D*RR AA YHPPIK
Sbjct: 629 LGD*RRQAALYHPPIK 676
Score = 29.9 bits (59), Expect(6) = 7e-052
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = +2 / -2
Query: 398 GWTTLPRPTENYYLWHRVD 454
G TTLPRPT +Y H VD
Sbjct: 441 GPTTLPRPTNYWYYHHCVD 385
Score = 29.5 bits (58), Expect(6) = 2e-056
Identities = 9/15 (60%), Positives = 10/15 (66%)
Frame = -2 / +2
Query: 271 WCGSVLLGCFGIGSG 227
WC LGCFG+G G
Sbjct: 566 WCVLAFLGCFGLGIG 610
Score = 29.5 bits (58), Expect(6) = 7e-052
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = +3 / -2
Query: 285 QHDKTRANTRPK 320
QHDKT ANTRPK
Sbjct: 561 QHDKTCANTRPK 526
Score = 21.7 bits (41), Expect(6) = 2e-056
Identities = 7/8 (87%), Positives = 7/8 (87%)
Frame = -3 / +2
Query: 159 GSHGDGLF 136
G HGDGLF
Sbjct: 683 GPHGDGLF 706
Score = 21.7 bits (41), Expect(6) = 7e-052
Identities = 7/9 (77%), Positives = 7/9 (77%)
Frame = +2 / -2
Query: 641 PQCATGPAT 667
P CAT PAT
Sbjct: 195 PSCATAPAT 169
Score = 21.7 bits (41), Expect(5) = 3e-023
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = -1 / +1
Query: 338 LGNYQ*FRPSISAG 297
LG FRPSISAG
Sbjct: 508 LGITSSFRPSISAG 549
>gb|CL177979.1|CL177979 104_385_10894229_148_31913_293 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10894229, DNA
sequence
Length = 731
Score = 128 bits (275), Expect(6) = 2e-056
Identities = 51/54 (94%), Positives = 53/54 (98%)
Frame = -3 / -2
Query: 648 HWGQNWQSNTLLDGQALSFRVTTSDGRSVVSSNAAPRGWGFGQTFSGAQFN*TP 487
+WGQNWQSNTLLDGQALSFRVTTSDGRSVVS+NAAPRGW FGQTFSGAQFN*TP
Sbjct: 706 NWGQNWQSNTLLDGQALSFRVTTSDGRSVVSNNAAPRGWAFGQTFSGAQFN*TP 545
Score = 127 bits (272), Expect(6) = 7e-052
Identities = 50/55 (90%), Positives = 51/55 (92%)
Frame = +2 / +3
Query: 482 MEGVQLNWAPLKVWPKPQPRGAALLETTERPSLVVTRKERACPSRSVLLCQFWPQ 646
+ GVQLNWAPLKVWPK QPRGAALLETTERPSLVVTR E ACPSRSVLLCQFWPQ
Sbjct: 540 VSGVQLNWAPLKVWPKAQPRGAALLETTERPSLVVTRNESACPSRSVLLCQFWPQ 704
Score = 53.8 bits (111), Expect(6) = 2e-056
Identities = 19/27 (70%), Positives = 21/27 (77%)
Frame = -2 / -3
Query: 400 PSLGACREAPWFYEWCISLWIWGITSS 320
P+ REAPWFYEWCIS W+ GITSS
Sbjct: 450 PNWSLPREAPWFYEWCISHWLLGITSS 370
Score = 47.3 bits (97), Expect(6) = 7e-052
Identities = 17/21 (80%), Positives = 18/21 (85%)
Frame = +1 / +3
Query: 319 NYW*FPRSKARCTIHKTMAPL 381
NYW*FP + ARCT HKTMAPL
Sbjct: 369 NYW*FPGANARCTTHKTMAPL 431
Score = 47.3 bits (97), Expect(5) = 3e-023
Identities = 26/47 (55%), Positives = 29/47 (61%)
Frame = -1 / -2
Query: 458 RRRRGATSSSFPLVVVESSTLTRSLPRGAMVL*MVHLALDLGNYQ*F 318
RR G+TSSS +V RGAMVL*+VHLAL GNYQ*F
Sbjct: 508 RRSGGSTSSSSVVVESSVHPQLELAERGAMVL*VVHLALAPGNYQ*F 368
Score = 46.9 bits (96), Expect(6) = 2e-056
Identities = 18/23 (78%), Positives = 21/23 (91%)
Frame = -2 / -2
Query: 223 KAGLQINGGRPPFITRQLSICGV 155
KAGL+INGGRPP ITRQLS+ G+
Sbjct: 274 KAGLEINGGRPPCITRQLSVLGL 206
Score = 45.1 bits (92), Expect(5) = 3e-023
Identities = 18/21 (85%), Positives = 18/21 (85%)
Frame = -2 / -1
Query: 544 PARLGLRPDLQRGPVQLNTLH 482
P RLGLRPDLQR PVQLNT H
Sbjct: 602 PPRLGLRPDLQRRPVQLNTRH 540
Score = 41.4 bits (84), Expect(6) = 7e-052
Identities = 21/34 (61%), Positives = 23/34 (67%)
Frame = +2 / +2
Query: 122 KHLIRKRPSP*DPTNA*LAGDKRRPTSVNLQASL 223
K + KR + NA*LAGD RRP SVNLQASL
Sbjct: 173 KTSLLKRGHHHEAQNA*LAGDTRRPASVNLQASL 274
Score = 36.8 bits (74), Expect(5) = 3e-023
Identities = 14/14 (100%), Positives = 14/14 (100%)
Frame = -2 / -1
Query: 646 LGPELAEQHAPGRA 605
LGPELAEQHAPGRA
Sbjct: 704 LGPELAEQHAPGRA 663
Score = 32.7 bits (65), Expect(6) = 2e-056
Identities = 12/18 (66%), Positives = 14/18 (77%)
Frame = -2 / -1
Query: 337 WGITSSLGLVLARVLSCW 284
W + LGLVLA+VLSCW
Sbjct: 386 WELPVVLGLVLAQVLSCW 333
Score = 31.8 bits (63), Expect(5) = 3e-023
Identities = 13/16 (81%), Positives = 13/16 (81%)
Frame = -1 / -1
Query: 215 LAD*RR*AAFYHPPIK 168
L D*RR AA YHPPIK
Sbjct: 266 LGD*RRQAALYHPPIK 219
Score = 29.9 bits (59), Expect(6) = 7e-052
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = +2 / +1
Query: 398 GWTTLPRPTENYYLWHRVD 454
G TTLPRPT +Y H VD
Sbjct: 454 GPTTLPRPTNYWYYHHCVD 510
Score = 29.5 bits (58), Expect(6) = 2e-056
Identities = 9/15 (60%), Positives = 10/15 (66%)
Frame = -2 / -1
Query: 271 WCGSVLLGCFGIGSG 227
WC LGCFG+G G
Sbjct: 329 WCVLAFLGCFGLGIG 285
Score = 29.5 bits (58), Expect(6) = 7e-052
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = +3 / +1
Query: 285 QHDKTRANTRPK 320
QHDKT ANTRPK
Sbjct: 334 QHDKTCANTRPK 369
Score = 21.7 bits (41), Expect(6) = 7e-052
Identities = 7/9 (77%), Positives = 7/9 (77%)
Frame = +2 / +1
Query: 641 PQCATGPAT 667
P CAT PAT
Sbjct: 700 PSCATAPAT 726
Score = 21.7 bits (41), Expect(6) = 2e-056
Identities = 7/8 (87%), Positives = 7/8 (87%)
Frame = -3 / -1
Query: 159 GSHGDGLF 136
G HGDGLF
Sbjct: 212 GPHGDGLF 189
Score = 21.7 bits (41), Expect(5) = 3e-023
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = -1 / -3
Query: 338 LGNYQ*FRPSISAG 297
LG FRPSISAG
Sbjct: 387 LGITSSFRPSISAG 346
>gb|CL177978.1|CL177978 104_385_10894229_116_31914_293 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10894229, DNA
sequence
Length = 704
Score = 128 bits (275), Expect(4) = 3e-045
Identities = 51/54 (94%), Positives = 53/54 (98%)
Frame = -3 / +1
Query: 648 HWGQNWQSNTLLDGQALSFRVTTSDGRSVVSSNAAPRGWGFGQTFSGAQFN*TP 487
+WGQNWQSNTLLDGQALSFRVTTSDGRSVVS+NAAPRGW FGQTFSGAQFN*TP
Sbjct: 295 NWGQNWQSNTLLDGQALSFRVTTSDGRSVVSNNAAPRGWAFGQTFSGAQFN*TP 456
Score = 127 bits (272), Expect(5) = 2e-044
Identities = 50/55 (90%), Positives = 51/55 (92%)
Frame = +2 / -1
Query: 482 MEGVQLNWAPLKVWPKPQPRGAALLETTERPSLVVTRKERACPSRSVLLCQFWPQ 646
+ GVQLNWAPLKVWPK QPRGAALLETTERPSLVVTR E ACPSRSVLLCQFWPQ
Sbjct: 461 VSGVQLNWAPLKVWPKAQPRGAALLETTERPSLVVTRNESACPSRSVLLCQFWPQ 297
Score = 53.8 bits (111), Expect(4) = 3e-045
Identities = 19/27 (70%), Positives = 21/27 (77%)
Frame = -2 / +2
Query: 400 PSLGACREAPWFYEWCISLWIWGITSS 320
P+ REAPWFYEWCIS W+ GITSS
Sbjct: 551 PNWSLPREAPWFYEWCISHWLLGITSS 631
Score = 47.3 bits (97), Expect(5) = 2e-044
Identities = 17/21 (80%), Positives = 18/21 (85%)
Frame = +1 / -1
Query: 319 NYW*FPRSKARCTIHKTMAPL 381
NYW*FP + ARCT HKTMAPL
Sbjct: 632 NYW*FPGANARCTTHKTMAPL 570
Score = 32.7 bits (65), Expect(4) = 3e-045
Identities = 12/18 (66%), Positives = 14/18 (77%)
Frame = -2 / +3
Query: 337 WGITSSLGLVLARVLSCW 284
W + LGLVLA+VLSCW
Sbjct: 615 WELPVVLGLVLAQVLSCW 668
Score = 29.9 bits (59), Expect(5) = 2e-044
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = +2 / -2
Query: 398 GWTTLPRPTENYYLWHRVD 454
G TTLPRPT +Y H VD
Sbjct: 547 GPTTLPRPTNYWYYHHCVD 491
Score = 29.5 bits (58), Expect(5) = 2e-044
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = +3 / -2
Query: 285 QHDKTRANTRPK 320
QHDKT ANTRPK
Sbjct: 667 QHDKTCANTRPK 632
Score = 24.0 bits (46), Expect(4) = 3e-045
Identities = 7/11 (63%), Positives = 7/11 (63%)
Frame = -2 / +3
Query: 271 WCGSVLLGCFG 239
WC LGCFG
Sbjct: 672 WCVLAFLGCFG 704
Score = 21.7 bits (41), Expect(5) = 2e-044
Identities = 7/9 (77%), Positives = 7/9 (77%)
Frame = +2 / -2
Query: 641 PQCATGPAT 667
P CAT PAT
Sbjct: 301 PSCATAPAT 275
>gb|CW325048.1|CW325048 104_819_11477484_116_35915_034 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11477484, DNA
sequence
Length = 612
Score = 128 bits (275), Expect(4) = 3e-045
Identities = 51/54 (94%), Positives = 53/54 (98%)
Frame = -3 / +1
Query: 648 HWGQNWQSNTLLDGQALSFRVTTSDGRSVVSSNAAPRGWGFGQTFSGAQFN*TP 487
+WGQNWQSNTLLDGQALSFRVTTSDGRSVVS+NAAPRGW FGQTFSGAQFN*TP
Sbjct: 202 NWGQNWQSNTLLDGQALSFRVTTSDGRSVVSNNAAPRGWAFGQTFSGAQFN*TP 363
Score = 127 bits (272), Expect(5) = 9e-045
Identities = 50/55 (90%), Positives = 51/55 (92%)
Frame = +2 / -2
Query: 482 MEGVQLNWAPLKVWPKPQPRGAALLETTERPSLVVTRKERACPSRSVLLCQFWPQ 646
+ GVQLNWAPLKVWPK QPRGAALLETTERPSLVVTR E ACPSRSVLLCQFWPQ
Sbjct: 368 VSGVQLNWAPLKVWPKAQPRGAALLETTERPSLVVTRNESACPSRSVLLCQFWPQ 204
Score = 53.8 bits (111), Expect(4) = 3e-045
Identities = 19/27 (70%), Positives = 21/27 (77%)
Frame = -2 / +2
Query: 400 PSLGACREAPWFYEWCISLWIWGITSS 320
P+ REAPWFYEWCIS W+ GITSS
Sbjct: 458 PNWSLPREAPWFYEWCISHWLLGITSS 538
Score = 47.3 bits (97), Expect(5) = 9e-045
Identities = 17/21 (80%), Positives = 18/21 (85%)
Frame = +1 / -2
Query: 319 NYW*FPRSKARCTIHKTMAPL 381
NYW*FP + ARCT HKTMAPL
Sbjct: 539 NYW*FPGANARCTTHKTMAPL 477
Score = 32.7 bits (65), Expect(4) = 3e-045
Identities = 12/18 (66%), Positives = 14/18 (77%)
Frame = -2 / +3
Query: 337 WGITSSLGLVLARVLSCW 284
W + LGLVLA+VLSCW
Sbjct: 522 WELPVVLGLVLAQVLSCW 575
Score = 29.9 bits (59), Expect(5) = 9e-045
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = +2 / -3
Query: 398 GWTTLPRPTENYYLWHRVD 454
G TTLPRPT +Y H VD
Sbjct: 454 GPTTLPRPTNYWYYHHCVD 398
Score = 29.5 bits (58), Expect(5) = 9e-045
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = +3 / -3
Query: 285 QHDKTRANTRPK 320
QHDKT ANTRPK
Sbjct: 574 QHDKTCANTRPK 539
Score = 24.0 bits (46), Expect(4) = 3e-045
Identities = 7/11 (63%), Positives = 7/11 (63%)
Frame = -2 / +3
Query: 271 WCGSVLLGCFG 239
WC LGCFG
Sbjct: 579 WCVLAFLGCFG 611
Score = 21.7 bits (41), Expect(5) = 9e-045
Identities = 7/9 (77%), Positives = 7/9 (77%)
Frame = +2 / -3
Query: 641 PQCATGPAT 667
P CAT PAT
Sbjct: 208 PSCATAPAT 182
>gb|BG322950.1|BG322950 EM1_13_B01.g1_A002 Embryo 1 (EM1) Sorghum bicolor cDNA, mRNA
sequence
Length = 518
Score = 86.3 bits (182), Expect(6) = 6e-045
Identities = 35/37 (94%), Positives = 36/37 (97%)
Frame = -3 / +1
Query: 597 SFRVTTSDGRSVVSSNAAPRGWGFGQTFSGAQFN*TP 487
SFRVTTSDGRSVVS+NAAPRGW FGQTFSGAQFN*TP
Sbjct: 1 SFRVTTSDGRSVVSNNAAPRGWAFGQTFSGAQFN*TP 111
Score = 85.4 bits (180), Expect(5) = 1e-038
Identities = 34/37 (91%), Positives = 35/37 (94%)
Frame = +2 / -1
Query: 482 MEGVQLNWAPLKVWPKPQPRGAALLETTERPSLVVTR 592
+ GVQLNWAPLKVWPK QPRGAALLETTERPSLVVTR
Sbjct: 116 VSGVQLNWAPLKVWPKAQPRGAALLETTERPSLVVTR 6
Score = 53.8 bits (111), Expect(6) = 6e-045
Identities = 19/27 (70%), Positives = 21/27 (77%)
Frame = -2 / +2
Query: 400 PSLGACREAPWFYEWCISLWIWGITSS 320
P+ REAPWFYEWCIS W+ GITSS
Sbjct: 206 PNWSLPREAPWFYEWCISHWLLGITSS 286
Score = 47.3 bits (97), Expect(5) = 1e-038
Identities = 17/21 (80%), Positives = 18/21 (85%)
Frame = +1 / -1
Query: 319 NYW*FPRSKARCTIHKTMAPL 381
NYW*FP + ARCT HKTMAPL
Sbjct: 287 NYW*FPGANARCTTHKTMAPL 225
Score = 46.9 bits (96), Expect(6) = 6e-045
Identities = 18/23 (78%), Positives = 21/23 (91%)
Frame = -2 / +1
Query: 223 KAGLQINGGRPPFITRQLSICGV 155
KAGL+INGGRPP ITRQLS+ G+
Sbjct: 382 KAGLEINGGRPPCITRQLSVLGL 450
Score = 41.4 bits (84), Expect(5) = 1e-038
Identities = 21/34 (61%), Positives = 23/34 (67%)
Frame = +2 / -3
Query: 122 KHLIRKRPSP*DPTNA*LAGDKRRPTSVNLQASL 223
K + KR + NA*LAGD RRP SVNLQASL
Sbjct: 483 KTSLLKRGHHHEAQNA*LAGDTRRPASVNLQASL 382
Score = 32.7 bits (65), Expect(6) = 6e-045
Identities = 12/18 (66%), Positives = 14/18 (77%)
Frame = -2 / +3
Query: 337 WGITSSLGLVLARVLSCW 284
W + LGLVLA+VLSCW
Sbjct: 270 WELPVVLGLVLAQVLSCW 323
Score = 29.9 bits (59), Expect(5) = 1e-038
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = +2 / -2
Query: 398 GWTTLPRPTENYYLWHRVD 454
G TTLPRPT +Y H VD
Sbjct: 202 GPTTLPRPTNYWYYHHCVD 146
Score = 29.5 bits (58), Expect(6) = 6e-045
Identities = 9/15 (60%), Positives = 10/15 (66%)
Frame = -2 / +3
Query: 271 WCGSVLLGCFGIGSG 227
WC LGCFG+G G
Sbjct: 327 WCVLAFLGCFGLGIG 371
Score = 29.5 bits (58), Expect(5) = 1e-038
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = +3 / -2
Query: 285 QHDKTRANTRPK 320
QHDKT ANTRPK
Sbjct: 322 QHDKTCANTRPK 287
Score = 21.7 bits (41), Expect(6) = 6e-045
Identities = 7/8 (87%), Positives = 7/8 (87%)
Frame = -3 / +3
Query: 159 GSHGDGLF 136
G HGDGLF
Sbjct: 444 GPHGDGLF 467
>gb|BG556478.1|BG556478 EM1_37_F06.g1_A002 Embryo 1 (EM1) Sorghum bicolor cDNA, mRNA
sequence
Length = 357
Score = 93.2 bits (197), Expect(4) = 3e-034
Identities = 37/42 (88%), Positives = 38/42 (90%)
Frame = +2 / -1
Query: 482 MEGVQLNWAPLKVWPKPQPRGAALLETTERPSLVVTRKERAC 607
+ GVQLNWAPLKVWPK QPRGAALLETTERPSLVVTR E AC
Sbjct: 126 VSGVQLNWAPLKVWPKAQPRGAALLETTERPSLVVTRNESAC 1
Score = 92.3 bits (195), Expect(5) = 2e-035
Identities = 38/40 (95%), Positives = 39/40 (97%)
Frame = -3 / +2
Query: 606 QALSFRVTTSDGRSVVSSNAAPRGWGFGQTFSGAQFN*TP 487
QALSFRVTTSDGRSVVS+NAAPRGW FGQTFSGAQFN*TP
Sbjct: 2 QALSFRVTTSDGRSVVSNNAAPRGWAFGQTFSGAQFN*TP 121
Score = 53.8 bits (111), Expect(5) = 2e-035
Identities = 19/27 (70%), Positives = 21/27 (77%)
Frame = -2 / +3
Query: 400 PSLGACREAPWFYEWCISLWIWGITSS 320
P+ REAPWFYEWCIS W+ GITSS
Sbjct: 216 PNWSLPREAPWFYEWCISHWLLGITSS 296
Score = 47.3 bits (97), Expect(4) = 3e-034
Identities = 17/21 (80%), Positives = 18/21 (85%)
Frame = +1 / -1
Query: 319 NYW*FPRSKARCTIHKTMAPL 381
NYW*FP + ARCT HKTMAPL
Sbjct: 297 NYW*FPGANARCTTHKTMAPL 235
Score = 32.7 bits (65), Expect(5) = 2e-035
Identities = 12/18 (66%), Positives = 14/18 (77%)
Frame = -2 / +1
Query: 337 WGITSSLGLVLARVLSCW 284
W + LGLVLA+VLSCW
Sbjct: 280 WELPVVLGLVLAQVLSCW 333
Score = 29.9 bits (59), Expect(4) = 3e-034
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = +2 / -2
Query: 398 GWTTLPRPTENYYLWHRVD 454
G TTLPRPT +Y H VD
Sbjct: 212 GPTTLPRPTNYWYYHHCVD 156
Score = 29.5 bits (58), Expect(4) = 3e-034
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = +3 / -2
Query: 285 QHDKTRANTRPK 320
QHDKT ANTRPK
Sbjct: 332 QHDKTCANTRPK 297
Score = 20.8 bits (39), Expect(5) = 2e-035
Identities = 8/9 (88%), Positives = 8/9 (88%)
Frame = -3 / +1
Query: 423 VGRGRVVHP 397
VGRGRVV P
Sbjct: 187 VGRGRVVGP 213
Score = 19.9 bits (37), Expect(5) = 2e-035
Identities = 7/8 (87%), Positives = 7/8 (87%)
Frame = -1 / +3
Query: 488 PPSFPQRR 465
P SFPQRR
Sbjct: 126 PRSFPQRR 149
>gb|CW325049.1|CW325049 104_819_11477484_148_35911_034 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11477484, DNA
sequence
Length = 672
Score = 53.8 bits (111), Expect(5) = 6e-024
Identities = 19/27 (70%), Positives = 21/27 (77%)
Frame = -2 / -1
Query: 400 PSLGACREAPWFYEWCISLWIWGITSS 320
P+ REAPWFYEWCIS W+ GITSS
Sbjct: 576 PNWSLPREAPWFYEWCISHWLLGITSS 496
Score = 46.9 bits (96), Expect(5) = 6e-024
Identities = 18/23 (78%), Positives = 21/23 (91%)
Frame = -2 / -3
Query: 223 KAGLQINGGRPPFITRQLSICGV 155
KAGL+INGGRPP ITRQLS+ G+
Sbjct: 400 KAGLEINGGRPPCITRQLSVLGL 332
Score = 32.7 bits (65), Expect(5) = 6e-024
Identities = 12/18 (66%), Positives = 14/18 (77%)
Frame = -2 / -2
Query: 337 WGITSSLGLVLARVLSCW 284
W + LGLVLA+VLSCW
Sbjct: 512 WELPVVLGLVLAQVLSCW 459
Score = 29.5 bits (58), Expect(5) = 6e-024
Identities = 9/15 (60%), Positives = 10/15 (66%)
Frame = -2 / -2
Query: 271 WCGSVLLGCFGIGSG 227
WC LGCFG+G G
Sbjct: 455 WCVLAFLGCFGLGIG 411
Score = 21.7 bits (41), Expect(5) = 6e-024
Identities = 7/8 (87%), Positives = 7/8 (87%)
Frame = -3 / -2
Query: 159 GSHGDGLF 136
G HGDGLF
Sbjct: 338 GPHGDGLF 315
>gb|CN151243.1|CN151243 WOUND1_74_G01.b1_A002 Wounded leaves Sorghum bicolor cDNA clone
WOUND1_74_G01_A002 3', mRNA sequence
Length = 681
Score = 101 bits (216), Expect = 9e-021
Identities = 39/50 (78%), Positives = 44/50 (88%)
Frame = -3 / +1
Query: 648 HWGQNWQSNTLLDGQALSFRVTTSDGRSVVSSNAAPRGWGFGQTFSGAQF 499
+WGQNWQSN LDGQALSF+VT+SDGR+V S+NAAP GW FGQTF GAQF
Sbjct: 130 NWGQNWQSNADLDGQALSFQVTSSDGRTVTSNNAAPAGWQFGQTFEGAQF 279
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 743,610,101
Number of Sequences: 832831
Number of extensions: 10709424
Number of successful extensions: 106909
Number of sequences better than 1.0e-020: 10
length of database: 163,786,556
effective HSP length: 54
effective length of database: 118,813,682
effective search space used: 19960698576
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)