TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2591368.2.1
(543 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CD425442.1|CD425442 SA1_12_C07.b1_A002 Salicylic acid-tr... 112 2e-041
gb|CW185910.1|CW185910 104_603_11166295_148_36698_020 Sorgh... 112 3e-035
>gb|CD425442.1|CD425442 SA1_12_C07.b1_A002 Salicylic acid-treated seedlings Sorghum bicolor
cDNA clone SA1_12_C07_A002 3', mRNA sequence
Length = 613
Score = 112 bits (239), Expect(4) = 2e-041
Identities = 44/59 (74%), Positives = 48/59 (81%)
Frame = -1 / +2
Query: 540 VKLNVSPGTSFHCNEPGWFRVCYANMDDDTMEVALDRIRRFVRQHQHSKAKAERWAATR 364
VKLNVSPGTSFHCNEPGWFRVC+ANMDDDTMEVAL RI FVRQHQ + + + A R
Sbjct: 77 VKLNVSPGTSFHCNEPGWFRVCHANMDDDTMEVALRRIHHFVRQHQQQQQQQSKAKAER 253
Score = 82.6 bits (174), Expect(4) = 6e-036
Identities = 32/47 (68%), Positives = 32/47 (68%)
Frame = +2 / -2
Query: 401 CWCWRTKRRIRXXXXXXXXXXMLA*QTRNQPGSLQWNDVPGDTLSFT 541
CWCWRTK I MLA QTRN PGSLQWNDVPGDTLSFT
Sbjct: 216 CWCWRTKWWILRSATSMVSSSMLAWQTRNHPGSLQWNDVPGDTLSFT 76
Score = 60.2 bits (125), Expect(4) = 2e-041
Identities = 24/36 (66%), Positives = 27/36 (75%)
Frame = -2 / +3
Query: 323 LRTSPSPAPWRCCRRSPRWSTPAS*SPSVR*DWLYG 216
LRTSPSPAPWRCCRR R + + SPS+R DWL G
Sbjct: 303 LRTSPSPAPWRCCRRRSRPWSTPAKSPSIRQDWL*G 410
Score = 57.4 bits (119), Expect(5) = 2e-025
Identities = 24/30 (80%), Positives = 26/30 (86%)
Frame = -1 / +2
Query: 414 RQHQHSKAKAERWAATRPLRLSLPRRGATT 325
+Q Q SKAKAERWAATR LRLSLPRRGA +
Sbjct: 218 QQQQQSKAKAERWAATRRLRLSLPRRGAAS 307
Score = 56.1 bits (116), Expect(4) = 6e-036
Identities = 24/37 (64%), Positives = 28/37 (75%)
Frame = +3 / -3
Query: 216 TVQPVLPNAR*LASWRGPSGTAATATPRGWGWRGAKR 326
T+QPVLPNAR*L+ + A TATPRGW WRGAK+
Sbjct: 410 TLQPVLPNAR*LSWRGPWATAATTATPRGWRWRGAKQ 300
Score = 54.7 bits (113), Expect(5) = 2e-025
Identities = 23/25 (92%), Positives = 24/25 (96%)
Frame = -3 / +1
Query: 541 GEAQRVARHVVPLQRARLVPRLLRQ 467
GEAQRVARHVVPLQRAR+VPRL RQ
Sbjct: 76 GEAQRVARHVVPLQRARMVPRLPRQ 150
Score = 35.0 bits (70), Expect(4) = 6e-036
Identities = 13/25 (52%), Positives = 18/25 (72%)
Frame = +1 / -2
Query: 109 PTPVQRLCVMSS*LRTPFISLV*YF 183
P P +RLC+ S+*LRTP S + +F
Sbjct: 561 PIPAERLCITSN*LRTPIYSCLIFF 487
Score = 33.6 bits (67), Expect(4) = 6e-036
Identities = 16/35 (45%), Positives = 17/35 (48%)
Frame = +2 / -2
Query: 305 GMARCEAVVAPXXXXXXXXXXVAAQRSALALLCWC 409
G+ AP VAAQRSALALLC C
Sbjct: 327 GLEMARCEAAPRRGKLRRRRRVAAQRSALALLCCC 223
Score = 29.0 bits (57), Expect(4) = 2e-041
Identities = 11/17 (64%), Positives = 13/17 (76%)
Frame = -2 / +2
Query: 158 GVRS*LDITQRRCTGVG 108
GVRS* D+ QRR G+G
Sbjct: 512 GVRS*FDVIQRRSAGIG 562
Score = 27.2 bits (53), Expect(5) = 2e-025
Identities = 11/12 (91%), Positives = 12/12 (100%)
Frame = -3 / +3
Query: 157 AYVASLTLHRDV 122
AYVASLTL+RDV
Sbjct: 513 AYVASLTLYRDV 548
Score = 24.4 bits (47), Expect(5) = 2e-025
Identities = 9/12 (75%), Positives = 9/12 (75%)
Frame = -2 / +3
Query: 290 CCRRSPRWSTPA 255
C RRS WSTPA
Sbjct: 339 CRRRSRPWSTPA 374
Score = 24.4 bits (47), Expect(4) = 2e-041
Identities = 9/10 (90%), Positives = 9/10 (90%)
Frame = -3 / +1
Query: 361 PPPQLAAPGS 332
P PQLAAPGS
Sbjct: 271 PAPQLAAPGS 300
Score = 23.1 bits (44), Expect(5) = 2e-025
Identities = 10/19 (52%), Positives = 12/19 (63%)
Frame = -1 / +2
Query: 249 VTERSVRLAVRCALT*LQT 193
VTE S RLAV C ++T
Sbjct: 377 VTEHSARLAVGCVCPYIRT 433
>gb|CW185910.1|CW185910 104_603_11166295_148_36698_020 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11166295, DNA
sequence
Length = 613
Score = 112 bits (239), Expect(4) = 3e-035
Identities = 44/59 (74%), Positives = 48/59 (81%)
Frame = -1 / -2
Query: 540 VKLNVSPGTSFHCNEPGWFRVCYANMDDDTMEVALDRIRRFVRQHQHSKAKAERWAATR 364
VKLNVSPGTSFHCNEPGWFRVC+ANMDDDTMEVAL RI FVRQHQ + + + A R
Sbjct: 303 VKLNVSPGTSFHCNEPGWFRVCHANMDDDTMEVALRRIHHFVRQHQQQQQQQSKAKAER 127
Score = 82.6 bits (174), Expect(3) = 1e-024
Identities = 32/47 (68%), Positives = 32/47 (68%)
Frame = +2 / +2
Query: 401 CWCWRTKRRIRXXXXXXXXXXMLA*QTRNQPGSLQWNDVPGDTLSFT 541
CWCWRTK I MLA QTRN PGSLQWNDVPGDTLSFT
Sbjct: 164 CWCWRTKWWILRSATSMVSSSMLAWQTRNHPGSLQWNDVPGDTLSFT 304
Score = 46.0 bits (94), Expect(4) = 3e-035
Identities = 16/18 (88%), Positives = 16/18 (88%)
Frame = -2 / -3
Query: 323 LRTSPSPAPWRCCRRSPR 270
LRTSPSPAPWRCCRR R
Sbjct: 77 LRTSPSPAPWRCCRRRSR 24
Score = 38.2 bits (77), Expect(3) = 1e-024
Identities = 13/16 (81%), Positives = 14/16 (87%)
Frame = +3 / +3
Query: 279 AATATPRGWGWRGAKR 326
A TATPRGW WRGAK+
Sbjct: 33 ATTATPRGWRWRGAKQ 80
Score = 30.9 bits (61), Expect(3) = 1e-024
Identities = 10/14 (71%), Positives = 13/14 (92%)
Frame = +2 / +2
Query: 251 S*LAWTIGDCGDSN 292
+*LAWT+GDCGD +
Sbjct: 2 T*LAWTMGDCGDDS 43
Score = 24.4 bits (47), Expect(4) = 3e-035
Identities = 9/10 (90%), Positives = 9/10 (90%)
Frame = -3 / -1
Query: 361 PPPQLAAPGS 332
P PQLAAPGS
Sbjct: 109 PAPQLAAPGS 80
Score = 22.1 bits (42), Expect(4) = 3e-035
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = -3 / -1
Query: 286 VAAVPDGPRQLA 251
VAAV GPRQL+
Sbjct: 37 VAAVAHGPRQLS 2
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 546,974,937
Number of Sequences: 832831
Number of extensions: 7533653
Number of successful extensions: 195723
Number of sequences better than 1.0e-020: 2
length of database: 163,786,556
effective HSP length: 53
effective length of database: 119,646,513
effective search space used: 15195107151
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)