TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2521918.2.1
(417 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CD428352.1|CD428352 ETH1_31_F04.b1_A002 Ethylene-treated... 153 4e-053
gb|CN141083.1|CN141083 OX1_49_E07.b1_A002 Oxidatively-stres... 153 2e-051
gb|AW747493.1|AW747493 WS1_68_E01.g1_A002 Water-stressed 1 ... 140 5e-035
gb|CL149549.1|CL149549 104_330_10777899_116_31354_219 Sorgh... 101 6e-021
gb|CW399413.1|CW399413 fsbb001f089d24f0 Sorghum methylation... 101 6e-021
>gb|CD428352.1|CD428352 ETH1_31_F04.b1_A002 Ethylene-treated seedlings Sorghum bicolor cDNA
clone ETH1_31_F04_A002 3', mRNA sequence
Length = 643
Score = 153 bits (329), Expect(3) = 4e-053
Identities = 61/63 (96%), Positives = 62/63 (98%)
Frame = +2 / +2
Query: 2 CVFRVSGPKFLKLNKGTWHAGPLFKADAVDFYNLELSNTNVVDHTTHHFKKHDGITFVVE 181
CVFRVSG KFLKLNKGTWHAGPLFKADAVDFYNLELSNTNVVDHTTH+FKKHDGITFVVE
Sbjct: 290 CVFRVSGSKFLKLNKGTWHAGPLFKADAVDFYNLELSNTNVVDHTTHYFKKHDGITFVVE 469
Query: 182 D*K 190
D*K
Sbjct: 470 D*K 478
Score = 143 bits (307), Expect(3) = 2e-049
Identities = 55/63 (87%), Positives = 57/63 (90%)
Frame = -2 / -3
Query: 191 FFSPPPQMLSHHVS*NGVWYDQQRWCCSVPDYRNQRRPP*TKARHARSPCSA*ETWGQKP 12
FFSPPPQMLSHHVS*N V YD+QRWCCS PDYRNQ+ PP*TKARHARSPCSA*ETW KP
Sbjct: 479 FFSPPPQMLSHHVS*NSV*YDRQRWCCSAPDYRNQQHPP*TKARHARSPCSA*ETWSLKP 300
Query: 11 GTR 3
GTR
Sbjct: 299 GTR 291
Score = 139 bits (299), Expect(2) = 5e-046
Identities = 56/63 (88%), Positives = 59/63 (93%)
Frame = -3 / -1
Query: 190 FLVLHHKCYPIMFLEMVCGMINNVGVAQFQIIEINGVRLEQRPGMPGPLVQLEKLGARNP 11
FLVLHHKCYPIMFLE+VC MI+NVGVAQ QIIEIN +RLEQRPGMPGPLVQLEKLGA NP
Sbjct: 478 FLVLHHKCYPIMFLEIVCSMIDNVGVAQLQIIEINSIRLEQRPGMPGPLVQLEKLGA*NP 299
Query: 10 EHA 2
EHA
Sbjct: 298 EHA 290
Score = 136 bits (292), Expect(2) = 6e-046
Identities = 57/67 (85%), Positives = 60/67 (89%)
Frame = +3 / +3
Query: 3 ACSGFLAPSFSS*TRGPGMPGLCSRRTPLISIIWN*ATPTLLIIPHTISRNMMG*HLWWR 182
ACSGF APSFSS*TRGPGMPGLCSRR LISIIW+*ATPTL II HTISRNMMG*HLWWR
Sbjct: 291 ACSGFQAPSFSS*TRGPGMPGLCSRRMLLISIIWS*ATPTLSIILHTISRNMMG*HLWWR 470
Query: 183 TKKRTSL 203
TKK ++
Sbjct: 471 TKKHFAI 491
Score = 127 bits (272), Expect(3) = 3e-039
Identities = 55/63 (87%), Positives = 56/63 (88%)
Frame = +1 / +1
Query: 1 LRVPGFWPQVSQAEQGDLACRAFVQGGRR*FL*SGTEQHQRC*SYHTPFQET*WDNICGG 180
LRVPGF QVSQAEQGDLACRAFVQGG *FL*SG EQHQRC SY+T FQET*WDNICGG
Sbjct: 289 LRVPGFRLQVSQAEQGDLACRAFVQGGCC*FL*SGAEQHQRCRSYYTLFQET*WDNICGG 468
Query: 181 GLK 189
GLK
Sbjct: 469 GLK 477
Score = 68.0 bits (142), Expect(2) = 6e-046
Identities = 30/45 (66%), Positives = 32/45 (71%)
Frame = +2 / +1
Query: 191 KNISQSEYH*QDRWLILSMFVVNNQHVKCHVEMRI*GPCSNLCIV 325
KNISQSE+H QD WLI FVVNNQHVKC+VEM I* C V
Sbjct: 475 KNISQSEHHCQDCWLIFYKFVVNNQHVKCYVEMHI*VQTWASCFV 609
Score = 67.0 bits (140), Expect(3) = 4e-053
Identities = 27/38 (71%), Positives = 30/38 (78%)
Frame = +1 / +3
Query: 190 KEHLSIRIPLTRPLADLVHVCSEQPTCKVSCRNAHLRS 303
K+H +IR L R LADL+ VCSEQPTCKV CRNAHL S
Sbjct: 474 KKHFAIRTSLPRLLADLLQVCSEQPTCKVLCRNAHLSS 587
Score = 67.0 bits (140), Expect(3) = 2e-049
Identities = 26/35 (74%), Positives = 32/35 (91%)
Frame = -3 / -2
Query: 298 SDAHFYMTLYMLVVHYKHGQDQPAVLSVVFGLRDV 194
SD HFY+TLYMLVVHYK +DQPAVL+++FGLR+V
Sbjct: 582 SDVHFYITLYMLVVHYKLVEDQPAVLAMMFGLRNV 478
Score = 66.1 bits (138), Expect(3) = 4e-030
Identities = 27/28 (96%), Positives = 27/28 (96%)
Frame = -1 / -2
Query: 84 ASALNKGPACQVPLFSLRNLGPETRNTQ 1
ASALNKGPACQVPLFSLRNL PETRNTQ
Sbjct: 372 ASALNKGPACQVPLFSLRNLEPETRNTQ 289
Score = 65.2 bits (136), Expect(2) = 5e-046
Identities = 30/45 (66%), Positives = 34/45 (75%)
Frame = -1 / -3
Query: 324 TIHKFEQGPQMRIST*HFTCWLFTTNMDKISQRSCQWYSD*EMFF 190
T H+ + QM IST*HFTCWLFTTN+ KISQ+S Q SD EMFF
Sbjct: 608 TKHEAQV*TQMCIST*HFTCWLFTTNL*KISQQSWQ*CSDCEMFF 474
Score = 64.3 bits (134), Expect(3) = 4e-030
Identities = 25/36 (69%), Positives = 30/36 (83%)
Frame = -2 / -1
Query: 302 DLRCAFLHDTLHVGCSLQTWTRSASGLVSGIRIERC 195
+LRCAFLH+TLHVGCSLQT RSAS L + +RI +C
Sbjct: 586 ELRCAFLHNTLHVGCSLQTCRRSASSLGNDVRIAKC 479
Score = 51.0 bits (105), Expect(3) = 3e-039
Identities = 26/36 (72%), Positives = 27/36 (75%)
Frame = +3 / +2
Query: 192 RTSLNPNTTDKTAG*SCPCL**TTNM*SVM*KCASE 299
+T NPN KTAG*S L**TTNM*SVM*KC SE
Sbjct: 476 KTFRNPNIIAKTAG*SSTSL**TTNM*SVM*KCTSE 583
Score = 39.1 bits (79), Expect(3) = 4e-030
Identities = 16/17 (94%), Positives = 16/17 (94%)
Frame = -1 / -2
Query: 189 F*SSTTNVIPSCFLKWC 139
F*SSTTNVIPSCFLK C
Sbjct: 477 F*SSTTNVIPSCFLK*C 427
Score = 26.7 bits (52), Expect(3) = 4e-053
Identities = 10/16 (62%), Positives = 12/16 (75%)
Frame = +3 / +1
Query: 306 VQTCVSCFVRTEIKAK 353
VQT SCFVRT +K +
Sbjct: 583 VQTWASCFVRTNMKGE 630
Score = 24.0 bits (46), Expect(3) = 2e-049
Identities = 11/19 (57%), Positives = 13/19 (68%)
Frame = -2 / -2
Query: 353 FCLYFGSNKARYTSLNKDL 297
F L+ GSNKA SLN D+
Sbjct: 630 FPLHVGSNKA*GPSLNSDV 574
Score = 22.1 bits (42), Expect(3) = 3e-039
Identities = 12/18 (66%), Positives = 12/18 (66%)
Frame = +1 / +2
Query: 301 SLFKLVYRALLEPK*RQK 354
S FKL ALLEP *R K
Sbjct: 578 SEFKLGPHALLEPT*RGK 631
>gb|CN141083.1|CN141083 OX1_49_E07.b1_A002 Oxidatively-stressed leaves and roots Sorghum
bicolor cDNA clone OX1_49_E07_A002 3', mRNA sequence
Length = 691
Score = 153 bits (329), Expect(3) = 2e-051
Identities = 61/63 (96%), Positives = 62/63 (98%)
Frame = +2 / +3
Query: 2 CVFRVSGPKFLKLNKGTWHAGPLFKADAVDFYNLELSNTNVVDHTTHHFKKHDGITFVVE 181
CVFRVSG KFLKLNKGTWHAGPLFKADAVDFYNLELSNTNVVDHTTH+FKKHDGITFVVE
Sbjct: 237 CVFRVSGSKFLKLNKGTWHAGPLFKADAVDFYNLELSNTNVVDHTTHYFKKHDGITFVVE 416
Query: 182 D*K 190
D*K
Sbjct: 417 D*K 425
Score = 144 bits (308), Expect(3) = 4e-047
Identities = 55/64 (85%), Positives = 58/64 (90%)
Frame = -2 / -2
Query: 194 SFFSPPPQMLSHHVS*NGVWYDQQRWCCSVPDYRNQRRPP*TKARHARSPCSA*ETWGQK 15
+FFSPPPQMLSHHVS*N V YD+QRWCCS PDYRNQ+ PP*TKARHARSPCSA*ETW K
Sbjct: 429 TFFSPPPQMLSHHVS*NSV*YDRQRWCCSAPDYRNQQHPP*TKARHARSPCSA*ETWSLK 250
Query: 14 PGTR 3
PGTR
Sbjct: 249 PGTR 238
Score = 139 bits (299), Expect(2) = 3e-045
Identities = 56/63 (88%), Positives = 59/63 (93%)
Frame = -3 / -3
Query: 190 FLVLHHKCYPIMFLEMVCGMINNVGVAQFQIIEINGVRLEQRPGMPGPLVQLEKLGARNP 11
FLVLHHKCYPIMFLE+VC MI+NVGVAQ QIIEIN +RLEQRPGMPGPLVQLEKLGA NP
Sbjct: 425 FLVLHHKCYPIMFLEIVCSMIDNVGVAQLQIIEINSIRLEQRPGMPGPLVQLEKLGA*NP 246
Query: 10 EHA 2
EHA
Sbjct: 245 EHA 237
Score = 138 bits (297), Expect(2) = 2e-046
Identities = 58/67 (86%), Positives = 61/67 (91%)
Frame = +3 / +1
Query: 3 ACSGFLAPSFSS*TRGPGMPGLCSRRTPLISIIWN*ATPTLLIIPHTISRNMMG*HLWWR 182
ACSGF APSFSS*TRGPGMPGLCSRR LISIIW+*ATPTL II HTISRNMMG*HLWWR
Sbjct: 238 ACSGFQAPSFSS*TRGPGMPGLCSRRMLLISIIWS*ATPTLSIILHTISRNMMG*HLWWR 417
Query: 183 TKKRTSL 203
TKKR ++
Sbjct: 418 TKKRFAI 438
Score = 127 bits (272), Expect(3) = 3e-037
Identities = 55/63 (87%), Positives = 56/63 (88%)
Frame = +1 / +2
Query: 1 LRVPGFWPQVSQAEQGDLACRAFVQGGRR*FL*SGTEQHQRC*SYHTPFQET*WDNICGG 180
LRVPGF QVSQAEQGDLACRAFVQGG *FL*SG EQHQRC SY+T FQET*WDNICGG
Sbjct: 236 LRVPGFRLQVSQAEQGDLACRAFVQGGCC*FL*SGAEQHQRCRSYYTLFQET*WDNICGG 415
Query: 181 GLK 189
GLK
Sbjct: 416 GLK 424
Score = 67.5 bits (141), Expect(2) = 2e-046
Identities = 29/45 (64%), Positives = 32/45 (71%)
Frame = +2 / +2
Query: 191 KNISQSEYH*QDRWLILSMFVVNNQHVKCHVEMRI*GPCSNLCIV 325
KN+SQSE+H QD WLI FVVNNQHVKC+VEM I* C V
Sbjct: 422 KNVSQSEHHCQDCWLIFYKFVVNNQHVKCYVEMHI*VQTWASCFV 556
Score = 66.1 bits (138), Expect(3) = 4e-028
Identities = 27/28 (96%), Positives = 27/28 (96%)
Frame = -1 / -1
Query: 84 ASALNKGPACQVPLFSLRNLGPETRNTQ 1
ASALNKGPACQVPLFSLRNL PETRNTQ
Sbjct: 319 ASALNKGPACQVPLFSLRNLEPETRNTQ 236
Score = 62.5 bits (130), Expect(2) = 3e-045
Identities = 29/45 (64%), Positives = 33/45 (73%)
Frame = -1 / -2
Query: 324 TIHKFEQGPQMRIST*HFTCWLFTTNMDKISQRSCQWYSD*EMFF 190
T H+ + QM IST*HFTCWLFTTN+ KISQ+S Q SD E FF
Sbjct: 555 TKHEAQV*TQMCIST*HFTCWLFTTNL*KISQQSWQ*CSDCETFF 421
Score = 62.0 bits (129), Expect(3) = 4e-047
Identities = 24/35 (68%), Positives = 32/35 (91%)
Frame = -3 / -1
Query: 298 SDAHFYMTLYMLVVHYKHGQDQPAVLSVVFGLRDV 194
S+ HFY+TLYMLVVHYK ++QPAVL+++FGLR+V
Sbjct: 529 SNVHFYITLYMLVVHYKLVKNQPAVLAMMFGLRNV 425
Score = 61.6 bits (128), Expect(3) = 2e-051
Identities = 25/38 (65%), Positives = 28/38 (73%)
Frame = +1 / +1
Query: 190 KEHLSIRIPLTRPLADLVHVCSEQPTCKVSCRNAHLRS 303
K+ +IR L R LAD + VCSEQPTCKV CRNAHL S
Sbjct: 421 KKRFAIRTSLPRLLADFLQVCSEQPTCKVLCRNAHLSS 534
Score = 57.4 bits (119), Expect(3) = 4e-028
Identities = 22/35 (62%), Positives = 29/35 (82%)
Frame = -2 / -3
Query: 302 DLRCAFLHDTLHVGCSLQTWTRSASGLVSGIRIER 198
+L+CAFLH+TLHVGCSLQT +SAS L + +RI +
Sbjct: 533 ELKCAFLHNTLHVGCSLQTCKKSASSLGNDVRIAK 429
Score = 45.1 bits (92), Expect(3) = 3e-037
Identities = 24/36 (66%), Positives = 25/36 (69%)
Frame = +3 / +3
Query: 192 RTSLNPNTTDKTAG*SCPCL**TTNM*SVM*KCASE 299
+T NPN KTAG* L**TTNM*SVM*KC E
Sbjct: 423 KTFRNPNIIAKTAG*FFTSL**TTNM*SVM*KCTFE 530
Score = 39.1 bits (79), Expect(3) = 4e-028
Identities = 16/17 (94%), Positives = 16/17 (94%)
Frame = -1 / -1
Query: 189 F*SSTTNVIPSCFLKWC 139
F*SSTTNVIPSCFLK C
Sbjct: 424 F*SSTTNVIPSCFLK*C 374
Score = 26.7 bits (52), Expect(3) = 2e-051
Identities = 10/16 (62%), Positives = 12/16 (75%)
Frame = +3 / +2
Query: 306 VQTCVSCFVRTEIKAK 353
VQT SCFVRT +K +
Sbjct: 530 VQTWASCFVRTNMKGE 577
Score = 21.7 bits (41), Expect(3) = 3e-037
Identities = 11/16 (68%), Positives = 11/16 (68%)
Frame = +1 / +3
Query: 307 FKLVYRALLEPK*RQK 354
FKL ALLEP *R K
Sbjct: 531 FKLGPHALLEPT*RGK 578
Score = 21.7 bits (41), Expect(3) = 4e-047
Identities = 10/19 (52%), Positives = 13/19 (68%)
Frame = -2 / -1
Query: 353 FCLYFGSNKARYTSLNKDL 297
F L+ GSNKA SLN ++
Sbjct: 577 FPLHVGSNKA*GPSLNSNV 521
>gb|AW747493.1|AW747493 WS1_68_E01.g1_A002 Water-stressed 1 (WS1) Sorghum bicolor cDNA,
mRNA sequence
Length = 218
Score = 140 bits (300), Expect(2) = 5e-035
Identities = 57/62 (91%), Positives = 58/62 (93%)
Frame = +2 / +1
Query: 5 VFRVSGPKFLKLNKGTWHAGPLFKADAVDFYNLELSNTNVVDHTTHHFKKHDGITFVVED 184
VFRVSG KFLKLNKGTWHAGPLFKADAVDFYN ELSNTNVVDH T +FKKHDGITFVVED
Sbjct: 1 VFRVSGSKFLKLNKGTWHAGPLFKADAVDFYNRELSNTNVVDHITRYFKKHDGITFVVED 180
Query: 185 *K 190
*K
Sbjct: 181 *K 186
Score = 139 bits (298), Expect = 2e-032
Identities = 53/62 (85%), Positives = 55/62 (88%)
Frame = -2 / -2
Query: 191 FFSPPPQMLSHHVS*NGVWYDQQRWCCSVPDYRNQRRPP*TKARHARSPCSA*ETWGQKP 12
FFSPPPQMLSHHVS*N V YD+QRWCCS PDYRNQ+ PP*TKARHARSPCS *ETW KP
Sbjct: 187 FFSPPPQMLSHHVS*NSV*YDRQRWCCSTPDYRNQQHPP*TKARHARSPCST*ETWSLKP 8
Query: 11 GT 6
GT
Sbjct: 7 GT 2
Score = 133 bits (285), Expect = 1e-030
Identities = 53/62 (85%), Positives = 56/62 (90%)
Frame = -3 / -3
Query: 190 FLVLHHKCYPIMFLEMVCGMINNVGVAQFQIIEINGVRLEQRPGMPGPLVQLEKLGARNP 11
FLVLHHKCYPIMFLE+ C MI+NVGVAQ IIEIN +RLEQRPGMPGPLVQLEKLGA NP
Sbjct: 186 FLVLHHKCYPIMFLEIACNMIDNVGVAQLPIIEINSIRLEQRPGMPGPLVQLEKLGA*NP 7
Query: 10 EH 5
EH
Sbjct: 6 EH 1
Score = 126 bits (270), Expect = 2e-028
Identities = 54/66 (81%), Positives = 57/66 (86%)
Frame = +3 / +2
Query: 6 CSGFLAPSFSS*TRGPGMPGLCSRRTPLISIIWN*ATPTLLIIPHTISRNMMG*HLWWRT 185
CSGF APSFSS*TRGPGMPGLCSRR LISII +*ATPTL II H ISRNMMG*HLWWRT
Sbjct: 2 CSGFQAPSFSS*TRGPGMPGLCSRRMLLISIIGS*ATPTLSIILHAISRNMMG*HLWWRT 181
Query: 186 KKRTSL 203
KK ++
Sbjct: 182 KKHFAI 199
Score = 121 bits (259), Expect = 5e-027
Identities = 52/61 (85%), Positives = 53/61 (86%)
Frame = +1 / +3
Query: 7 VPGFWPQVSQAEQGDLACRAFVQGGRR*FL*SGTEQHQRC*SYHTPFQET*WDNICGGGL 186
VPGF QVSQ EQGDLACRAFVQGG *FL*SG EQHQRC SY+T FQET*WDNICGGGL
Sbjct: 3 VPGFRLQVSQVEQGDLACRAFVQGGCC*FL*SGVEQHQRCRSYYTLFQET*WDNICGGGL 182
Query: 187 K 189
K
Sbjct: 183 K 185
Score = 25.8 bits (50), Expect(2) = 5e-035
Identities = 10/12 (83%), Positives = 11/12 (91%)
Frame = +2 / +3
Query: 191 KNISQSEYH*QD 226
KNISQSE+H QD
Sbjct: 183 KNISQSEHHCQD 218
>gb|CL149549.1|CL149549 104_330_10777899_116_31354_219 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10777899, DNA
sequence
Length = 743
Score = 101 bits (215), Expect = 6e-021
Identities = 40/41 (97%), Positives = 40/41 (97%)
Frame = +2 / -2
Query: 2 CVFRVSGPKFLKLNKGTWHAGPLFKADAVDFYNLELSNTNV 124
CVFRVSG KFLKLNKGTWHAGPLFKADAVDFYNLELSNTNV
Sbjct: 592 CVFRVSGSKFLKLNKGTWHAGPLFKADAVDFYNLELSNTNV 470
>gb|CW399413.1|CW399413 fsbb001f089d24f0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f089d24, DNA
sequence
Length = 674
Score = 101 bits (215), Expect = 6e-021
Identities = 40/41 (97%), Positives = 40/41 (97%)
Frame = +2 / -3
Query: 2 CVFRVSGPKFLKLNKGTWHAGPLFKADAVDFYNLELSNTNV 124
CVFRVSG KFLKLNKGTWHAGPLFKADAVDFYNLELSNTNV
Sbjct: 495 CVFRVSGSKFLKLNKGTWHAGPLFKADAVDFYNLELSNTNV 373
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 561,691,046
Number of Sequences: 832831
Number of extensions: 8144783
Number of successful extensions: 175023
Number of sequences better than 1.0e-020: 5
length of database: 163,786,556
effective HSP length: 52
effective length of database: 120,479,344
effective search space used: 10361223584
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)