TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2493535.2.1
(580 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG240594.1|BG240594 OV1_31_G10.b1_A002 Ovary 1 (OV1) Sor... 155 2e-038
gb|CW498898.1|CW498898 fsbb001f293f22k0 Sorghum methylation... 122 9e-029
gb|CF758757.1|CF758757 DSAF1_36_C06.g1_A011 Drought-stresse... 100 6e-022
>gb|BG240594.1|BG240594 OV1_31_G10.b1_A002 Ovary 1 (OV1) Sorghum bicolor cDNA, mRNA
sequence
Length = 510
Score = 155 bits (332), Expect(2) = 2e-038
Identities = 67/85 (78%), Positives = 69/85 (81%)
Frame = +3 / -1
Query: 261 QCVVQKQRRRKPRHLPPGNPSVLQTLAQLDRRRQGPRNLWLCDWHVRASATATGPTSADS 440
QCV QKQRRRKPRHLP G PSVLQTLAQ RR QGPRNLWLCDWHV ASATAT P S +
Sbjct: 255 QCVRQKQRRRKPRHLPSGKPSVLQTLAQFSRRTQGPRNLWLCDWHVLASATATAPISVAT 76
Query: 441 SSSMTRRTAVKSLRESSISS*LGSQ 515
SSSMTR TAVKSLRESSI+ SQ
Sbjct: 75 SSSMTRTTAVKSLRESSIAPVPSSQ 1
Score = 105 bits (224), Expect(2) = 1e-023
Identities = 43/58 (74%), Positives = 47/58 (81%)
Frame = -1 / +2
Query: 493 MELSRKLFTAXXXXXXXXLSAEVGPVAVAEARTCQSQSHRFRGPCLRRSNCANVCRTE 320
MELSRKLFTA ++ E+G VAVAEARTCQSQSHRFRGPC+RR NCANVCRTE
Sbjct: 23 MELSRKLFTAVVLVMLLLVATEIGAVAVAEARTCQSQSHRFRGPCVRRENCANVCRTE 196
Score = 25.4 bits (49), Expect(2) = 2e-038
Identities = 11/21 (52%), Positives = 12/21 (57%)
Frame = +2 / -2
Query: 185 HGAMDWSRRARSTATPAGRWA 247
HG D RRAR +P GR A
Sbjct: 356 HGNRDRKRRARRMHSPPGRHA 294
Score = 24.9 bits (48), Expect(2) = 1e-023
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = -2 / +1
Query: 237 PAGVAVDRARRDQSMAPC 184
P G + RARR +S+ PC
Sbjct: 304 PGGECMRRARRFRSLLPC 357
>gb|CW498898.1|CW498898 fsbb001f293f22k0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f293f22, DNA
sequence
Length = 689
Score = 122 bits (261), Expect(2) = 9e-029
Identities = 49/58 (84%), Positives = 49/58 (84%)
Frame = +3 / +1
Query: 261 QCVVQKQRRRKPRHLPPGNPSVLQTLAQLDRRRQGPRNLWLCDWHVRASATATGPTSA 434
QCV QKQRRRKPRHLP G PSVLQTLAQ RR QGPRNLWLCDWHV ASATAT P SA
Sbjct: 400 QCVRQKQRRRKPRHLPSGKPSVLQTLAQFSRRTQGPRNLWLCDWHVLASATATAPISA 573
Score = 25.4 bits (49), Expect(2) = 9e-029
Identities = 11/21 (52%), Positives = 12/21 (57%)
Frame = +2 / +2
Query: 185 HGAMDWSRRARSTATPAGRWA 247
HG D RRAR +P GR A
Sbjct: 299 HGNRDRKRRARRMHSPPGRHA 361
>gb|CF758757.1|CF758757 DSAF1_36_C06.g1_A011 Drought-stressed after flowering Sorghum
bicolor cDNA clone DSAF1_36_C06_A011 3', mRNA sequence
Length = 505
Score = 99.6 bits (211), Expect(2) = 6e-022
Identities = 38/44 (86%), Positives = 38/44 (86%)
Frame = +3 / -1
Query: 261 QCVVQKQRRRKPRHLPPGNPSVLQTLAQLDRRRQGPRNLWLCDW 392
QCV QKQRRRKPRHLP G PSVLQTLAQ RR QGPRNLWLCDW
Sbjct: 133 QCVRQKQRRRKPRHLPSGKPSVLQTLAQFSRRTQGPRNLWLCDW 2
Score = 25.4 bits (49), Expect(2) = 6e-022
Identities = 11/21 (52%), Positives = 12/21 (57%)
Frame = +2 / -2
Query: 185 HGAMDWSRRARSTATPAGRWA 247
HG D RRAR +P GR A
Sbjct: 234 HGNRDRKRRARRMHSPPGRHA 172
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 516,695,967
Number of Sequences: 832831
Number of extensions: 6050516
Number of successful extensions: 97686
Number of sequences better than 1.0e-020: 3
length of database: 163,786,556
effective HSP length: 53
effective length of database: 119,646,513
effective search space used: 16630865307
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)