TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2440612.2.2
(665 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BE356001.1|BE356001 DG1_121_H09.b1_A002 Dark Grown 1 (DG... 167 2e-040
gb|BE360380.1|BE360380 DG1_63_G06.b1_A002 Dark Grown 1 (DG1... 167 2e-040
gb|BE360555.1|BE360555 DG1_64_D02.b1_A002 Dark Grown 1 (DG1... 167 2e-040
gb|BE360587.1|BE360587 DG1_64_D02.g1_A002 Dark Grown 1 (DG1... 167 2e-040
gb|CW199279.1|CW199279 104_624_11183428_116_36852_010 Sorgh... 157 2e-037
gb|BU672128.1|BU672128 pRC-1198 pRC Sorghum halepense cDNA ... 121 7e-027
gb|CW317085.1|CW317085 104_808_11473228_148_35858_004 Sorgh... 112 4e-024
gb|CX618902.1|CX618902 GABR1_42_H02.b1_A002 GA- or brassino... 110 3e-023
gb|CX618987.1|CX618987 GABR1_42_H02.g1_A002 GA- or brassino... 110 3e-023
gb|BG048866.1|BG048866 OV1_24_A12.b1_A002 Ovary 1 (OV1) Sor... 98 7e-022
>gb|BE356001.1|BE356001 DG1_121_H09.b1_A002 Dark Grown 1 (DG1) Sorghum bicolor cDNA, mRNA
sequence
Length = 516
Score = 167 bits (359), Expect = 2e-040
Identities = 72/95 (75%), Positives = 74/95 (77%)
Frame = -2 / +2
Query: 532 RRMAAPXXXXXXXXVATELGTTKVAEARHCLSQSHRFKGLCMSSNNCANVCQTENFPGGE 353
R AAP VATELGTT VAEARHCLSQSHRFKGLCMSS NCANVCQTENFPGGE
Sbjct: 71 RMAAAPFLVLVLLLVATELGTTPVAEARHCLSQSHRFKGLCMSSTNCANVCQTENFPGGE 250
Query: 352 CKAEGATRKCFCKKIC***PRLCLLAQAGRRHATQ 248
CKAEGATRKCFCKKIC* L ++GRRHA Q
Sbjct: 251 CKAEGATRKCFCKKIC*LVLALADRGRSGRRHAIQ 355
Score = 140 bits (301), Expect = 2e-032
Identities = 57/71 (80%), Positives = 59/71 (83%)
Frame = +3 / -1
Query: 252 VACRRPACASKQSRGYY*HIFLQKHLRVAPSALHSPPGKFSVWHTLAQLLLLMHRPLNRW 431
+ACRRP * IFLQKHLRVAPSALHSPPGKFSVWHTLAQL+LLMHRPLNRW
Sbjct: 351 IACRRPDLPLSAKASTS*QIFLQKHLRVAPSALHSPPGKFSVWHTLAQLVLLMHRPLNRW 172
Query: 432 LCDRQCLASAT 464
LCDRQCLASAT
Sbjct: 171 LCDRQCLASAT 139
Score = 138 bits (297), Expect = 6e-032
Identities = 61/76 (80%), Positives = 63/76 (82%)
Frame = -1 / +1
Query: 476 GDDQGGGGEALPVAEPPVQGPVHEQQQLRQRVPDRELPRRRVQGGGRHAQVLLQEDMLVV 297
GDD GGGGEALPVAEPPVQ PVHEQ QLRQRVPDRELPRRRVQG GRHAQVLLQED+L
Sbjct: 127 GDDSGGGGEALPVAEPPVQRPVHEQHQLRQRVPDRELPRRRVQGRGRHAQVLLQEDLLAS 306
Query: 296 ASALLAGTSRSSARNA 249
AS G RSSA N+
Sbjct: 307 ASLGGQGQVRSSACNS 354
>gb|BE360380.1|BE360380 DG1_63_G06.b1_A002 Dark Grown 1 (DG1) Sorghum bicolor cDNA, mRNA
sequence
Length = 553
Score = 167 bits (359), Expect = 2e-040
Identities = 72/95 (75%), Positives = 74/95 (77%)
Frame = -2 / +3
Query: 532 RRMAAPXXXXXXXXVATELGTTKVAEARHCLSQSHRFKGLCMSSNNCANVCQTENFPGGE 353
R AAP VATELGTT VAEARHCLSQSHRFKGLCMSS NCANVCQTENFPGGE
Sbjct: 66 RMAAAPFLVLVLLLVATELGTTPVAEARHCLSQSHRFKGLCMSSTNCANVCQTENFPGGE 245
Query: 352 CKAEGATRKCFCKKIC***PRLCLLAQAGRRHATQ 248
CKAEGATRKCFCKKIC* L ++GRRHA Q
Sbjct: 246 CKAEGATRKCFCKKIC*LVLALADRGRSGRRHAIQ 350
Score = 140 bits (301), Expect = 2e-032
Identities = 57/71 (80%), Positives = 59/71 (83%)
Frame = +3 / -1
Query: 252 VACRRPACASKQSRGYY*HIFLQKHLRVAPSALHSPPGKFSVWHTLAQLLLLMHRPLNRW 431
+ACRRP * IFLQKHLRVAPSALHSPPGKFSVWHTLAQL+LLMHRPLNRW
Sbjct: 346 IACRRPDLPLSAKASTS*QIFLQKHLRVAPSALHSPPGKFSVWHTLAQLVLLMHRPLNRW 167
Query: 432 LCDRQCLASAT 464
LCDRQCLASAT
Sbjct: 166 LCDRQCLASAT 134
Score = 138 bits (297), Expect = 6e-032
Identities = 61/76 (80%), Positives = 63/76 (82%)
Frame = -1 / +2
Query: 476 GDDQGGGGEALPVAEPPVQGPVHEQQQLRQRVPDRELPRRRVQGGGRHAQVLLQEDMLVV 297
GDD GGGGEALPVAEPPVQ PVHEQ QLRQRVPDRELPRRRVQG GRHAQVLLQED+L
Sbjct: 122 GDDSGGGGEALPVAEPPVQRPVHEQHQLRQRVPDRELPRRRVQGRGRHAQVLLQEDLLAS 301
Query: 296 ASALLAGTSRSSARNA 249
AS G RSSA N+
Sbjct: 302 ASLGGQGQVRSSACNS 349
>gb|BE360555.1|BE360555 DG1_64_D02.b1_A002 Dark Grown 1 (DG1) Sorghum bicolor cDNA, mRNA
sequence
Length = 509
Score = 167 bits (359), Expect = 2e-040
Identities = 72/95 (75%), Positives = 74/95 (77%)
Frame = -2 / +2
Query: 532 RRMAAPXXXXXXXXVATELGTTKVAEARHCLSQSHRFKGLCMSSNNCANVCQTENFPGGE 353
R AAP VATELGTT VAEARHCLSQSHRFKGLCMSS NCANVCQTENFPGGE
Sbjct: 71 RMAAAPFLVLVLLLVATELGTTPVAEARHCLSQSHRFKGLCMSSTNCANVCQTENFPGGE 250
Query: 352 CKAEGATRKCFCKKIC***PRLCLLAQAGRRHATQ 248
CKAEGATRKCFCKKIC* L ++GRRHA Q
Sbjct: 251 CKAEGATRKCFCKKIC*LVLALADRGRSGRRHAIQ 355
Score = 140 bits (301), Expect = 2e-032
Identities = 57/71 (80%), Positives = 59/71 (83%)
Frame = +3 / -3
Query: 252 VACRRPACASKQSRGYY*HIFLQKHLRVAPSALHSPPGKFSVWHTLAQLLLLMHRPLNRW 431
+ACRRP * IFLQKHLRVAPSALHSPPGKFSVWHTLAQL+LLMHRPLNRW
Sbjct: 351 IACRRPDLPLSAKASTS*QIFLQKHLRVAPSALHSPPGKFSVWHTLAQLVLLMHRPLNRW 172
Query: 432 LCDRQCLASAT 464
LCDRQCLASAT
Sbjct: 171 LCDRQCLASAT 139
Score = 138 bits (297), Expect = 6e-032
Identities = 61/76 (80%), Positives = 63/76 (82%)
Frame = -1 / +1
Query: 476 GDDQGGGGEALPVAEPPVQGPVHEQQQLRQRVPDRELPRRRVQGGGRHAQVLLQEDMLVV 297
GDD GGGGEALPVAEPPVQ PVHEQ QLRQRVPDRELPRRRVQG GRHAQVLLQED+L
Sbjct: 127 GDDSGGGGEALPVAEPPVQRPVHEQHQLRQRVPDRELPRRRVQGRGRHAQVLLQEDLLAS 306
Query: 296 ASALLAGTSRSSARNA 249
AS G RSSA N+
Sbjct: 307 ASLGGQGQVRSSACNS 354
>gb|BE360587.1|BE360587 DG1_64_D02.g1_A002 Dark Grown 1 (DG1) Sorghum bicolor cDNA, mRNA
sequence
Length = 356
Score = 167 bits (359), Expect(2) = 2e-040
Identities = 72/95 (75%), Positives = 74/95 (77%)
Frame = -2 / +2
Query: 532 RRMAAPXXXXXXXXVATELGTTKVAEARHCLSQSHRFKGLCMSSNNCANVCQTENFPGGE 353
R AAP VATELGTT VAEARHCLSQSHRFKGLCMSS NCANVCQTENFPGGE
Sbjct: 71 RMAAAPFLVLVLLLVATELGTTPVAEARHCLSQSHRFKGLCMSSTNCANVCQTENFPGGE 250
Query: 352 CKAEGATRKCFCKKIC***PRLCLLAQAGRRHATQ 248
CKAEGATRKCFCKKIC* L ++GRRHA Q
Sbjct: 251 CKAEGATRKCFCKKIC*LVLALADRGRSGRRHAIQ 355
Score = 140 bits (301), Expect(2) = 2e-032
Identities = 57/71 (80%), Positives = 59/71 (83%)
Frame = +3 / -3
Query: 252 VACRRPACASKQSRGYY*HIFLQKHLRVAPSALHSPPGKFSVWHTLAQLLLLMHRPLNRW 431
+ACRRP * IFLQKHLRVAPSALHSPPGKFSVWHTLAQL+LLMHRPLNRW
Sbjct: 351 IACRRPDLPLSAKASTS*QIFLQKHLRVAPSALHSPPGKFSVWHTLAQLVLLMHRPLNRW 172
Query: 432 LCDRQCLASAT 464
LCDRQCLASAT
Sbjct: 171 LCDRQCLASAT 139
Score = 138 bits (297), Expect = 6e-032
Identities = 61/76 (80%), Positives = 63/76 (82%)
Frame = -1 / +1
Query: 476 GDDQGGGGEALPVAEPPVQGPVHEQQQLRQRVPDRELPRRRVQGGGRHAQVLLQEDMLVV 297
GDD GGGGEALPVAEPPVQ PVHEQ QLRQRVPDRELPRRRVQG GRHAQVLLQED+L
Sbjct: 127 GDDSGGGGEALPVAEPPVQRPVHEQHQLRQRVPDRELPRRRVQGRGRHAQVLLQEDLLAS 306
Query: 296 ASALLAGTSRSSARNA 249
AS G RSSA N+
Sbjct: 307 ASLGGQGQVRSSACNS 354
Score = 19.4 bits (36), Expect(2) = 2e-040
Identities = 12/22 (54%), Positives = 12/22 (54%)
Frame = -2 / +2
Query: 583 KLRASGCQLPRGVMALSRRMAA 518
KLRA QL MALS R A
Sbjct: 14 KLRAGVVQLRVKGMALSSRRMA 79
Score = 18.9 bits (35), Expect(2) = 2e-032
Identities = 5/9 (55%), Positives = 8/9 (88%)
Frame = +2 / -2
Query: 524 HASRQRHHP 550
HA+R++ HP
Sbjct: 76 HAARRQRHP 50
>gb|CW199279.1|CW199279 104_624_11183428_116_36852_010 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11183428, DNA
sequence
Length = 624
Score = 157 bits (337), Expect = 2e-037
Identities = 64/76 (84%), Positives = 66/76 (86%)
Frame = -2 / +3
Query: 481 ELGTTKVAEARHCLSQSHRFKGLCMSSNNCANVCQTENFPGGECKAEGATRKCFCKKIC* 302
ELGTT VAEARHCLSQSHRFKGLCMSS NCANVCQTENFPGGECKAEGATRKCFCKKIC*
Sbjct: 384 ELGTTPVAEARHCLSQSHRFKGLCMSSTNCANVCQTENFPGGECKAEGATRKCFCKKIC* 563
Query: 301 **PRLCLLAQAGRRHA 254
L ++GRRHA
Sbjct: 564 LVLALADRGRSGRRHA 611
Score = 139 bits (298), Expect = 4e-032
Identities = 57/70 (81%), Positives = 58/70 (82%)
Frame = +3 / -3
Query: 255 ACRRPACASKQSRGYY*HIFLQKHLRVAPSALHSPPGKFSVWHTLAQLLLLMHRPLNRWL 434
ACRRP * IFLQKHLRVAPSALHSPPGKFSVWHTLAQL+LLMHRPLNRWL
Sbjct: 610 ACRRPDLPLSAKASTS*QIFLQKHLRVAPSALHSPPGKFSVWHTLAQLVLLMHRPLNRWL 431
Query: 435 CDRQCLASAT 464
CDRQCLASAT
Sbjct: 430 CDRQCLASAT 401
Score = 137 bits (293), Expect = 2e-031
Identities = 60/73 (82%), Positives = 61/73 (83%)
Frame = -1 / +2
Query: 476 GDDQGGGGEALPVAEPPVQGPVHEQQQLRQRVPDRELPRRRVQGGGRHAQVLLQEDMLVV 297
GDD GGGGEALPVAEPPVQ PVHEQ QLRQRVPDRELPRRRVQG GRHAQVLLQED+L
Sbjct: 389 GDDSGGGGEALPVAEPPVQRPVHEQHQLRQRVPDRELPRRRVQGRGRHAQVLLQEDLLAS 568
Query: 296 ASALLAGTSRSSA 258
AS G RSSA
Sbjct: 569 ASLGGQGQVRSSA 607
>gb|BU672128.1|BU672128 pRC-1198 pRC Sorghum halepense cDNA 5', mRNA sequence
Length = 423
Score = 121 bits (258), Expect(2) = 7e-027
Identities = 46/47 (97%), Positives = 46/47 (97%)
Frame = -2 / +1
Query: 442 LSQSHRFKGLCMSSNNCANVCQTENFPGGECKAEGATRKCFCKKIC* 302
LSQSHRFKGLCMSS NCANVCQTENFPGGECKAEGATRKCFCKKIC*
Sbjct: 1 LSQSHRFKGLCMSSTNCANVCQTENFPGGECKAEGATRKCFCKKIC* 141
Score = 112 bits (240), Expect = 4e-024
Identities = 44/46 (95%), Positives = 45/46 (97%)
Frame = +3 / -2
Query: 303 *HIFLQKHLRVAPSALHSPPGKFSVWHTLAQLLLLMHRPLNRWLCD 440
* IFLQKHLRVAPSALHSPPGKFSVWHTLAQL+LLMHRPLNRWLCD
Sbjct: 140 *QIFLQKHLRVAPSALHSPPGKFSVWHTLAQLVLLMHRPLNRWLCD 3
Score = 101 bits (216), Expect = 9e-021
Identities = 42/48 (87%), Positives = 44/48 (91%)
Frame = -1 / +3
Query: 440 VAEPPVQGPVHEQQQLRQRVPDRELPRRRVQGGGRHAQVLLQEDMLVV 297
VAEPPVQ PVHEQ QLRQRVPDRELPRRRVQG GRHAQVLLQED+L +
Sbjct: 3 VAEPPVQRPVHEQHQLRQRVPDRELPRRRVQGRGRHAQVLLQEDLLAL 146
Score = 21.7 bits (41), Expect(2) = 7e-027
Identities = 11/36 (30%), Positives = 14/36 (38%)
Frame = -3 / +3
Query: 279 WHKQVVGTQRNSSISVLLPRCCLLDPVFSFDRFRCF 172
W G + S L CC L V S+ + CF
Sbjct: 165 WRAGKAGRRHAIQASRLADHCCQLLLVLSYVCWFCF 272
>gb|CW317085.1|CW317085 104_808_11473228_148_35858_004 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11473228, DNA
sequence
Length = 712
Score = 112 bits (240), Expect = 4e-024
Identities = 41/60 (68%), Positives = 48/60 (80%)
Frame = -2 / +2
Query: 481 ELGTTKVAEARHCLSQSHRFKGLCMSSNNCANVCQTENFPGGECKAEGATRKCFCKKIC* 302
E+GTT+VAEARHCLSQSH+F G C+S NCANVC+TE FP GEC+ G KC CK+IC*
Sbjct: 140 EMGTTRVAEARHCLSQSHKFVGACLSKRNCANVCRTEGFPWGECRWHGIESKCHCKRIC* 319
>gb|CX618902.1|CX618902 GABR1_42_H02.b1_A002 GA- or brassinolide-treated seedlings Sorghum
bicolor cDNA clone GABR1_42_H02_A002 3', mRNA sequence
Length = 667
Score = 110 bits (234), Expect = 3e-023
Identities = 43/77 (55%), Positives = 53/77 (68%)
Frame = -2 / +1
Query: 532 RRMAAPXXXXXXXXVATELGTTKVAEARHCLSQSHRFKGLCMSSNNCANVCQTENFPGGE 353
R +A+ A+E+GTT+VAEARHCLSQSH+F G C+S NCANVC+TE FP GE
Sbjct: 109 RMVASSVLVFLLLLAASEMGTTRVAEARHCLSQSHKFVGACLSKRNCANVCRTEGFPWGE 288
Query: 352 CKAEGATRKCFCKKIC* 302
+ G KC CK+IC*
Sbjct: 289 RRWHGIESKCHCKRIC* 339
>gb|CX618987.1|CX618987 GABR1_42_H02.g1_A002 GA- or brassinolide-treated seedlings Sorghum
bicolor cDNA clone GABR1_42_H02_A002 5', mRNA sequence
Length = 562
Score = 110 bits (234), Expect = 3e-023
Identities = 43/77 (55%), Positives = 53/77 (68%)
Frame = -2 / +1
Query: 532 RRMAAPXXXXXXXXVATELGTTKVAEARHCLSQSHRFKGLCMSSNNCANVCQTENFPGGE 353
R +A+ A+E+GTT+VAEARHCLSQSH+F G C+S NCANVC+TE FP GE
Sbjct: 91 RMVASSVLVFLLLLAASEMGTTRVAEARHCLSQSHKFVGACLSKRNCANVCRTEGFPWGE 270
Query: 352 CKAEGATRKCFCKKIC* 302
+ G KC CK+IC*
Sbjct: 271 RRWHGIESKCHCKRIC* 321
>gb|BG048866.1|BG048866 OV1_24_A12.b1_A002 Ovary 1 (OV1) Sorghum bicolor cDNA, mRNA
sequence
Length = 402
Score = 97.7 bits (207), Expect(2) = 7e-022
Identities = 34/54 (62%), Positives = 40/54 (74%)
Frame = -2 / +3
Query: 463 VAEARHCLSQSHRFKGLCMSSNNCANVCQTENFPGGECKAEGATRKCFCKKIC* 302
V E +HCLSQSH F G+C S NC NVC+TE F GG+CK +G +RKCFC K C*
Sbjct: 138 VVEEKHCLSQSHSFSGMCFSHTNCDNVCKTEKFTGGKCKMDGTSRKCFCLKFC* 299
Score = 27.2 bits (53), Expect(2) = 7e-022
Identities = 15/31 (48%), Positives = 16/31 (51%)
Frame = -2 / +3
Query: 553 RGVMALSRRMAAPXXXXXXXXVATELGTTKV 461
R MAL RRMAAP VA E T +V
Sbjct: 45 RREMALCRRMAAPFFLVLLIIVAAETATARV 137
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 775,925,472
Number of Sequences: 832831
Number of extensions: 11688429
Number of successful extensions: 149376
Number of sequences better than 1.0e-020: 10
length of database: 163,786,556
effective HSP length: 54
effective length of database: 118,813,682
effective search space used: 19841884894
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)