TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCM12g02.yg.2.1
(480 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AI166267.1|AI166267 xylem.est.116 Poplar xylem Lambda ZA... 220 6e-057
gb|BU896476.1|BU896476 X041D11 Populus wood cDNA library Po... 212 1e-054
gb|AC149540.1| Populus trichocarpa clone Pop1-111J19, compl... 73 2e-039
gb|AJ770061.1|AJ770061 AJ770061 Populus euphratica leaf 3-6... 159 1e-038
gb|AJ776567.1|AJ776567 AJ776567 Populus euphratica root 3-6... 154 3e-037
gb|AJ772088.1|AJ772088 AJ772088 Populus euphratica shoot in... 151 2e-036
gb|CF232861.1|CF232861 PtaJXO0016F7F0711 Poplar cDNA librar... 126 1e-028
gb|CK116341.1|CK116341 B010P30 Hybrid aspen plasmid library... 107 5e-023
>gb|AI166267.1|AI166267 xylem.est.116 Poplar xylem Lambda ZAPII library Populus trichocarpa
cDNA 5', mRNA sequence
Length = 645
Score = 220 bits (474), Expect = 6e-057
Identities = 83/149 (55%), Positives = 110/149 (73%)
Frame = -3 / +1
Query: 448 QFIIQLGFFTALPMIIENSLEQGFLPAIWDFFTMQMNFSSVFYTFSMGTKSHYYGRTILH 269
Q +Q+GF ALPM++E LE+GF A+ +F MQ+ + VF+TFS+GTK+HYYGRT+LH
Sbjct: 85 QSFVQIGFLMALPMLMEIGLERGFRTALSEFLLMQLQLAPVFFTFSLGTKTHYYGRTLLH 264
Query: 268 GGAKYRATGRGFVVQHKSFAENYRLYARGHFIKAIELGIILTVYAAHSVIAKNTLVYIXX 89
GGAKYR+TGRGFVV H FA+NYRLY+R HF+K IE+ I+L VY ++ + Y+
Sbjct: 265 GGAKYRSTGRGFVVFHAKFADNYRLYSRSHFVKGIEMMILLVVYQIFGQPYRSAVAYVLI 444
Query: 88 XISSWFLVVSWIMAPFAFNPSGFDWLKTV 2
IS WF+V +W+ APF FNPSGF+W K V
Sbjct: 445 TISMWFMVGTWLFAPFLFNPSGFEWQKIV 531
Score = 90.0 bits (190), Expect(2) = 1e-022
Identities = 46/79 (58%), Positives = 49/79 (62%)
Frame = +3 / -2
Query: 195 SL*FSAKLLCCTTKPRPVARYFAPP*RIVRP**WLLVPMENV*NTDEKFICIVKKSQIAG 374
SL* SA L TTKP PV YFAPP V P**W+ VP ENV NT CI K S A
Sbjct: 338 SL*LSANLAWKTTKPLPVDLYFAPPCSNVLP**WVFVPRENVKNTGASCNCINKNSLSAV 159
Query: 375 RKPCSREFSIIIGNAVKKP 431
RKP S+ SI IGNA+K P
Sbjct: 158 RKPLSKPISINIGNAIKNP 102
Score = 35.9 bits (72), Expect(2) = 1e-022
Identities = 17/38 (44%), Positives = 21/38 (55%)
Frame = +3 / -2
Query: 3 TVFNQSKPDGLKANGAIIHDTTRNQLDIXXXM*TSVFL 116
T+F SKP+GLK NGA T N +DI * + L
Sbjct: 530 TIFCHSKPEGLKRNGAKSQVPTINHIDIVIRT*ATALL 417
>gb|BU896476.1|BU896476 X041D11 Populus wood cDNA library Populus tremula x Populus
tremuloides cDNA 5 prime, mRNA sequence
Length = 467
Score = 212 bits (458), Expect = 1e-054
Identities = 81/145 (55%), Positives = 105/145 (72%)
Frame = -3 / +2
Query: 448 QFIIQLGFFTALPMIIENSLEQGFLPAIWDFFTMQMNFSSVFYTFSMGTKSHYYGRTILH 269
Q +Q+GF ALPM++E LE+GF A+ DF MQ+ + VF+TFS+GTK+HYYGRT+LH
Sbjct: 32 QSFVQIGFLMALPMMMEIGLEKGFRNALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLH 211
Query: 268 GGAKYRATGRGFVVQHKSFAENYRLYARGHFIKAIELGIILTVYAAHSVIAKNTLVYIXX 89
GG+ YRATGRGFVV H FA+NYRLY+R HF+K IEL I+L V+ + Y+
Sbjct: 212 GGSAYRATGRGFVVFHAKFADNYRLYSRSHFVKGIELMILLLVFHIFGRSYRGVFAYVLI 391
Query: 88 XISSWFLVVSWIMAPFAFNPSGFDW 14
IS WF+V +W+ APF FNPSGF+W
Sbjct: 392 TISMWFMVGTWLFAPFLFNPSGFEW 466
Score = 94.1 bits (199), Expect(2) = 1e-022
Identities = 51/95 (53%), Positives = 55/95 (57%)
Frame = +3 / -3
Query: 153 IPSSIAFMKWPLAYSL*FSAKLLCCTTKPRPVARYFAPP*RIVRP**WLLVPMENV*NTD 332
I +SI KW Y+L* SA L TTKPRPVA Y PP V P** + VP ENV NT
Sbjct: 327 IINSIPLTKWLREYNL*LSANLAWNTTKPRPVALYAEPPCSNVLP***VFVPRENVKNTG 148
Query: 333 EKFICIVKKSQIAGRKPCSREFSIIIGNAVKKPSC 437
CI KS A R P SR SIIIG A+K P C
Sbjct: 147 ANCNCINMKSLNALRNPFSRPISIIIGKAIKNPIC 43
Score = 31.3 bits (62), Expect(2) = 1e-022
Identities = 13/23 (56%), Positives = 14/23 (60%)
Frame = +3 / -3
Query: 18 SKPDGLKANGAIIHDTTRNQLDI 86
S PDGLK GA H T N +DI
Sbjct: 462 SNPDGLKRKGAKSHVPTMNHIDI 394
>gb|AC149540.1| Populus trichocarpa clone Pop1-111J19, complete sequence
Length = 96953
Score = 72.5 bits (152), Expect(3) = 2e-039
Identities = 27/40 (67%), Positives = 34/40 (85%)
Frame = -3 / +2
Query: 376 LPAIWDFFTMQMNFSSVFYTFSMGTKSHYYGRTILHGGAK 257
L A+ +F TMQ+ SVF+TFS+GTKSHY+GRTILHGGA+
Sbjct: 27902 LQAVVNFITMQLQLCSVFFTFSLGTKSHYFGRTILHGGAR 28021
Score = 68.9 bits (144), Expect(3) = 2e-039
Identities = 24/53 (45%), Positives = 35/53 (66%)
Frame = -3 / +2
Query: 166 IELGIILTVYAAHSVIAKNTLVYIXXXISSWFLVVSWIMAPFAFNPSGFDWLK 8
+E+ ++L VY A+ L YI +SSWF+ +SW+ AP+ FNPSGF+W K
Sbjct: 28280 LEVVLLLVVYLAYGYNDGGALSYILLTVSSWFMALSWLFAPYLFNPSGFEWQK 28438
Score = 58.8 bits (122), Expect(3) = 2e-039
Identities = 21/29 (72%), Positives = 26/29 (89%)
Frame = -3 / +3
Query: 256 YRATGRGFVVQHKSFAENYRLYARGHFIK 170
Y+ATGRGFVV+H F+ENYRLY+R HF+K
Sbjct: 28110 YQATGRGFVVRHIRFSENYRLYSRSHFVK 28196
>gb|AJ770061.1|AJ770061 AJ770061 Populus euphratica leaf 3-6 months, adult Populus
euphratica cDNA clone P0001100006E01F1, mRNA sequence
Length = 295
Score = 159 bits (342), Expect = 1e-038
Identities = 61/98 (62%), Positives = 80/98 (81%)
Frame = -3 / -1
Query: 433 LGFFTALPMIIENSLEQGFLPAIWDFFTMQMNFSSVFYTFSMGTKSHYYGRTILHGGAKY 254
+G FTA+PM++ LE GFL A+ F TMQ+ SVF+TFS+GTKSHY+GRTILHGGA+Y
Sbjct: 295 IGIFTAVPMVLGFILELGFLRAVVSFITMQLQLCSVFFTFSLGTKSHYFGRTILHGGARY 116
Query: 253 RATGRGFVVQHKSFAENYRLYARGHFIKAIELGIILTV 140
+ATGRGFVV+H F+ENYRLY+R HF+K +E+ ++L V
Sbjct: 115 QATGRGFVVRHIRFSENYRLYSRSHFVKGLEVVLLLVV 2
>gb|AJ776567.1|AJ776567 AJ776567 Populus euphratica root 3-6 months Populus euphratica cDNA
clone P0000400015B12F1, mRNA sequence
Length = 468
Score = 154 bits (331), Expect = 3e-037
Identities = 59/95 (62%), Positives = 75/95 (78%)
Frame = -3 / +3
Query: 448 QFIIQLGFFTALPMIIENSLEQGFLPAIWDFFTMQMNFSSVFYTFSMGTKSHYYGRTILH 269
Q +Q+GF ALPM++E LE+GF A+ DF MQ+ + VF+TFS+GTK+HYYGRT+LH
Sbjct: 183 QSFVQIGFLMALPMMMEIGLEKGFHNALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLH 362
Query: 268 GGAKYRATGRGFVVQHKSFAENYRLYARGHFIKAI 164
GG+ YRATGRGFVV H FA+NYRLY+R HF+K I
Sbjct: 363 GGSAYRATGRGFVVFHAKFADNYRLYSRSHFVKGI 467
>gb|AJ772088.1|AJ772088 AJ772088 Populus euphratica shoot in vitro plantlet Israel Populus
euphratica cDNA clone P0001700001C10F1, mRNA sequence
Length = 341
Score = 151 bits (325), Expect = 2e-036
Identities = 57/100 (57%), Positives = 74/100 (74%)
Frame = -3 / -2
Query: 340 NFSSVFYTFSMGTKSHYYGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARGHFIKAIE 161
N+ VF+TFS+GTK+HYYGRT+LHGG+ YRATGRGFVV H FA+NYRLY+R HF+K IE
Sbjct: 304 NWPPVFFTFSLGTKTHYYGRTLLHGGSAYRATGRGFVVFHAKFADNYRLYSRSHFVKGIE 125
Query: 160 LGIILTVYAAHSVIAKNTLVYIXXXISSWFLVVSWIMAPF 41
L I+L V+ + + Y+ IS WF+V +W+ APF
Sbjct: 124 LMILLLVFHIFGRSYRGVVAYVLITISMWFMVGTWLFAPF 5
>gb|CF232861.1|CF232861 PtaJXO0016F7F0711 Poplar cDNA library from young opposite xylem
Populus alba x Populus tremula cDNA 5', mRNA sequence
Length = 737
Score = 126 bits (269), Expect = 1e-028
Identities = 48/86 (55%), Positives = 60/86 (69%)
Frame = -3 / +2
Query: 259 KYRATGRGFVVQHKSFAENYRLYARGHFIKAIELGIILTVYAAHSVIAKNTLVYIXXXIS 80
KYR TGRGFVV H FAENYRLY+R HF+K IE+ I+L VY ++ + Y+ IS
Sbjct: 2 KYRPTGRGFVVFHAKFAENYRLYSRSHFVKGIEMMILLVVYQIFGQPYRSAVAYLLITIS 181
Query: 79 SWFLVVSWIMAPFAFNPSGFDWLKTV 2
WF+V +W+ APF FNPSGF+W K V
Sbjct: 182 MWFMVGTWLFAPFLFNPSGFEWQKIV 259
>gb|CK116341.1|CK116341 B010P30 Hybrid aspen plasmid library Populus tremula x Populus
tremuloides cDNA clone B010P30 5', mRNA sequence
Length = 297
Score = 107 bits (228), Expect = 5e-023
Identities = 42/78 (53%), Positives = 52/78 (66%)
Frame = -3 / +1
Query: 256 YRATGRGFVVQHKSFAENYRLYARGHFIKAIELGIILTVYAAHSVIAKNTLVYIXXXISS 77
YRATG GFVV H FA+NYRLY+R HF+K IEL I+L V+ + Y+ IS
Sbjct: 7 YRATGCGFVVFHAKFADNYRLYSRSHFVKGIELMILLLVFHIFGRSYRGVFAYVLITISM 186
Query: 76 WFLVVSWIMAPFAFNPSG 23
WF+V +W+ APF FNPSG
Sbjct: 187 WFMVGTWLFAPFLFNPSG 240
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 246,460,150
Number of Sequences: 369679
Number of extensions: 3583808
Number of successful extensions: 77720
Number of sequences better than 1.0e-020: 9
length of database: 67,802,888
effective HSP length: 50
effective length of database: 49,318,938
effective search space used: 5375764242
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)