TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCL28h07.yg.2.1
(277 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AI166267.1|AI166267 xylem.est.116 Poplar xylem Lambda ZA... 183 3e-046
gb|BU896476.1|BU896476 X041D11 Populus wood cDNA library Po... 181 1e-045
gb|AJ772088.1|AJ772088 AJ772088 Populus euphratica shoot in... 167 2e-042
gb|AJ776567.1|AJ776567 AJ776567 Populus euphratica root 3-6... 145 7e-035
gb|AJ770061.1|AJ770061 AJ770061 Populus euphratica leaf 3-6... 141 1e-033
gb|CF232861.1|CF232861 PtaJXO0016F7F0711 Poplar cDNA librar... 114 1e-025
gb|AC149540.1| Populus trichocarpa clone Pop1-111J19, compl... 67 5e-025
gb|CK116341.1|CK116341 B010P30 Hybrid aspen plasmid library... 109 6e-024
>gb|AI166267.1|AI166267 xylem.est.116 Poplar xylem Lambda ZAPII library Populus trichocarpa
cDNA 5', mRNA sequence
Length = 645
Score = 183 bits (394), Expect = 3e-046
Identities = 73/85 (85%), Positives = 81/85 (95%)
Frame = -1 / +1
Query: 256 VLMQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHF 77
+LMQLQLA VFFTFSLGTKTHYYG TLLHGGA+YR+TGRGFVVFHAKFA+NYRLYSRSHF
Sbjct: 178 LLMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAKYRSTGRGFVVFHAKFADNYRLYSRSHF 357
Query: 76 VKGIELMILLIVYEIFGQSYRGAIA 2
VKGIE+MILL+VY+IFGQ YR A+A
Sbjct: 358 VKGIEMMILLVVYQIFGQPYRSAVA 432
>gb|BU896476.1|BU896476 X041D11 Populus wood cDNA library Populus tremula x Populus
tremuloides cDNA 5 prime, mRNA sequence
Length = 467
Score = 181 bits (389), Expect = 1e-045
Identities = 73/85 (85%), Positives = 79/85 (92%)
Frame = -1 / +2
Query: 256 VLMQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHF 77
+LMQLQLA VFFTFSLGTKTHYYG TLLHGG+ YRATGRGFVVFHAKFA+NYRLYSRSHF
Sbjct: 125 ILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGSAYRATGRGFVVFHAKFADNYRLYSRSHF 304
Query: 76 VKGIELMILLIVYEIFGQSYRGAIA 2
VKGIELMILL+V+ IFG+SYRG A
Sbjct: 305 VKGIELMILLLVFHIFGRSYRGVFA 379
Score = 88.6 bits (187), Expect(2) = 2e-023
Identities = 37/52 (71%), Positives = 41/52 (78%)
Frame = +3 / -3
Query: 3 AIAPRYDCPNISYTISKIISSMPFTKWLREYNR*FSANLAWNTTNPRPVALY 158
A P Y+ PNI T S+II+S+P TKWLREYN * SANLAWNTT PRPVALY
Sbjct: 378 ANTPLYERPNIWNTRSRIINSIPLTKWLREYNL*LSANLAWNTTKPRPVALY 223
Score = 36.8 bits (74), Expect(2) = 2e-023
Identities = 15/20 (75%), Positives = 17/20 (85%)
Frame = +3 / -3
Query: 195 *VLVPRENVKNTDANWSCIS 254
*V VPRENVKNT AN +CI+
Sbjct: 186 *VFVPRENVKNTGANCNCIN 127
>gb|AJ772088.1|AJ772088 AJ772088 Populus euphratica shoot in vitro plantlet Israel Populus
euphratica cDNA clone P0001700001C10F1, mRNA sequence
Length = 341
Score = 167 bits (359), Expect(2) = 2e-042
Identities = 66/76 (86%), Positives = 72/76 (94%)
Frame = -1 / -2
Query: 229 VFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHFVKGIELMIL 50
VFFTFSLGTKTHYYG TLLHGG+ YRATGRGFVVFHAKFA+NYRLYSRSHFVKGIELMIL
Sbjct: 292 VFFTFSLGTKTHYYGRTLLHGGSAYRATGRGFVVFHAKFADNYRLYSRSHFVKGIELMIL 113
Query: 49 LIVYEIFGQSYRGAIA 2
L+V+ IFG+SYRG +A
Sbjct: 112 LLVFHIFGRSYRGVVA 65
Score = 89.5 bits (189), Expect(2) = 6e-021
Identities = 37/52 (71%), Positives = 41/52 (78%)
Frame = +3 / +3
Query: 3 AIAPRYDCPNISYTISKIISSMPFTKWLREYNR*FSANLAWNTTNPRPVALY 158
A P Y+ PNI T S+II+S+P TKWLREYN * SANLAWNTT PRPVALY
Sbjct: 66 ATTPLYERPNIWNTRSRIINSIPLTKWLREYNL*LSANLAWNTTKPRPVALY 221
Score = 26.7 bits (52), Expect(2) = 6e-021
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = +3 / +3
Query: 195 *VLVPRENVKNT 230
*V VPRENVKNT
Sbjct: 258 *VFVPRENVKNT 293
Score = 21.2 bits (40), Expect(2) = 2e-042
Identities = 8/12 (66%), Positives = 10/12 (83%)
Frame = -1 / -1
Query: 256 VLMQLQLASVFF 221
+LMQLQLA+ F
Sbjct: 320 ILMQLQLAACIF 285
>gb|AJ776567.1|AJ776567 AJ776567 Populus euphratica root 3-6 months Populus euphratica cDNA
clone P0000400015B12F1, mRNA sequence
Length = 468
Score = 145 bits (311), Expect = 7e-035
Identities = 58/64 (90%), Positives = 61/64 (95%)
Frame = -1 / +3
Query: 256 VLMQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHF 77
+LMQLQLA VFFTFSLGTKTHYYG TLLHGG+ YRATGRGFVVFHAKFA+NYRLYSRSHF
Sbjct: 276 ILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGSAYRATGRGFVVFHAKFADNYRLYSRSHF 455
Query: 76 VKGI 65
VKGI
Sbjct: 456 VKGI 467
>gb|AJ770061.1|AJ770061 AJ770061 Populus euphratica leaf 3-6 months, adult Populus
euphratica cDNA clone P0001100006E01F1, mRNA sequence
Length = 295
Score = 141 bits (302), Expect = 1e-033
Identities = 54/72 (75%), Positives = 66/72 (91%)
Frame = -1 / -1
Query: 256 VLMQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHF 77
+ MQLQL SVFFTFSLGTK+HY+G T+LHGGA Y+ATGRGFVV H +F+ENYRLYSRSHF
Sbjct: 217 ITMQLQLCSVFFTFSLGTKSHYFGRTILHGGARYQATGRGFVVRHIRFSENYRLYSRSHF 38
Query: 76 VKGIELMILLIV 41
VKG+E+++LL+V
Sbjct: 37 VKGLEVVLLLVV 2
>gb|CF232861.1|CF232861 PtaJXO0016F7F0711 Poplar cDNA library from young opposite xylem
Populus alba x Populus tremula cDNA 5', mRNA sequence
Length = 737
Score = 114 bits (244), Expect = 1e-025
Identities = 45/53 (84%), Positives = 50/53 (94%)
Frame = -1 / +2
Query: 160 EYRATGRGFVVFHAKFAENYRLYSRSHFVKGIELMILLIVYEIFGQSYRGAIA 2
+YR TGRGFVVFHAKFAENYRLYSRSHFVKGIE+MILL+VY+IFGQ YR A+A
Sbjct: 2 KYRPTGRGFVVFHAKFAENYRLYSRSHFVKGIEMMILLVVYQIFGQPYRSAVA 160
>gb|AC149540.1| Populus trichocarpa clone Pop1-111J19, complete sequence
Length = 96953
Score = 66.6 bits (139), Expect(2) = 5e-025
Identities = 25/32 (78%), Positives = 29/32 (90%)
Frame = -1 / +2
Query: 256 VLMQLQLASVFFTFSLGTKTHYYGTTLLHGGA 161
+ MQLQL SVFFTFSLGTK+HY+G T+LHGGA
Sbjct: 27923 ITMQLQLCSVFFTFSLGTKSHYFGRTILHGGA 28018
Score = 65.7 bits (137), Expect(2) = 5e-025
Identities = 25/30 (83%), Positives = 28/30 (93%)
Frame = -1 / +3
Query: 157 YRATGRGFVVFHAKFAENYRLYSRSHFVKG 68
Y+ATGRGFVV H +F+ENYRLYSRSHFVKG
Sbjct: 28110 YQATGRGFVVRHIRFSENYRLYSRSHFVKG 28199
>gb|CK116341.1|CK116341 B010P30 Hybrid aspen plasmid library Populus tremula x Populus
tremuloides cDNA clone B010P30 5', mRNA sequence
Length = 297
Score = 109 bits (232), Expect = 6e-024
Identities = 44/52 (84%), Positives = 48/52 (92%)
Frame = -1 / +1
Query: 157 YRATGRGFVVFHAKFAENYRLYSRSHFVKGIELMILLIVYEIFGQSYRGAIA 2
YRATG GFVVFHAKFA+NYRLYSRSHFVKGIELMILL+V+ IFG+SYRG A
Sbjct: 7 YRATGCGFVVFHAKFADNYRLYSRSHFVKGIELMILLLVFHIFGRSYRGVFA 162
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 133,004,665
Number of Sequences: 369679
Number of extensions: 1545688
Number of successful extensions: 134422
Number of sequences better than 1.0e-020: 9
length of database: 67,802,888
effective HSP length: 48
effective length of database: 50,058,296
effective search space used: 2152506728
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)