TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCH10g11.yg.2.1
(632 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DT497112.1|DT497112 WS01125.BR_I08 PT-P-FL-A-2 Populus t... 176 1e-071
gb|DT495294.1|DT495294 WS01120.BR_A19 PT-P-FL-A-2 Populus t... 176 1e-071
gb|DT493588.1|DT493588 WS0111.BR_N04 PT-P-FL-A-2 Populus tr... 176 1e-071
gb|AC149540.1| Populus trichocarpa clone Pop1-111J19, compl... 85 7e-057
>gb|DT497112.1|DT497112 WS01125.BR_I08 PT-P-FL-A-2 Populus trichocarpa cDNA clone
WS01125_I08 5', mRNA sequence
Length = 908
Score = 176 bits (379), Expect(2) = 1e-071
Identities = 69/86 (80%), Positives = 80/86 (93%)
Frame = -3 / +2
Query: 261 GIRKPKILGVREHVFTGSVSSLAWFMSAQETSFVTLGQRVLADPLKVRMHYGHPDVFDRL 82
G+R P ILG+REH+FTGSVSSLAWFMS QETSFVT+GQR+LA+PLKVR HYGHPDVFDRL
Sbjct: 302 GVRNPSILGLREHIFTGSVSSLAWFMSNQETSFVTIGQRLLANPLKVRFHYGHPDVFDRL 481
Query: 81 WFLGRGGISKASRIINISEDIFAGFN 4
+ L RGG+SKAS++IN+SEDIFAGFN
Sbjct: 482 FHLTRGGVSKASKVINLSEDIFAGFN 559
Score = 113 bits (242), Expect(2) = 1e-071
Identities = 44/56 (78%), Positives = 50/56 (89%)
Frame = -3 / +2
Query: 441 IYRVKLPGELKIGEGKPENQNHALIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEF 274
IYR+KLPG +GEGKPENQNHA+IFTRG+ +Q IDMNQDNY EEALKMRNLL+EF
Sbjct: 119 IYRIKLPGPAILGEGKPENQNHAIIFTRGEGLQAIDMNQDNYMEEALKMRNLLQEF 286
Score = 81.7 bits (172), Expect(2) = 3e-025
Identities = 43/76 (56%), Positives = 50/76 (65%)
Frame = +2 / -3
Query: 2 QLKPAKMSSLMLIILDALLIPPRPKNQRRSKTSGWP*CILTLSGSARTRCPRVTKLVSWA 181
+LKPA +SSL LI L+ALL PP K + S TSG P* TL G A + P VTKLVS
Sbjct: 561 ELKPANISSLRLITLEALLTPPLVK*KSLSNTSG*P*WNRTLRGLANSL*PIVTKLVS*F 382
Query: 182 DMNQAREETEPVKTCS 229
D+N AR+ET PV CS
Sbjct: 381 DINHARDETLPVNICS 334
Score = 53.3 bits (110), Expect(2) = 3e-025
Identities = 19/38 (50%), Positives = 24/38 (63%)
Frame = +1 / -2
Query: 289 VSHL*SFFKVVVLVHINSLNCIAPCEDECMILIFWLSF 402
+ H FF VV+LVHI L + CED M+L+FW SF
Sbjct: 271 IPHFQGFFHVVILVHIYCLQAFSTCEDNSMVLVFWFSF 158
>gb|DT495294.1|DT495294 WS01120.BR_A19 PT-P-FL-A-2 Populus trichocarpa cDNA clone
WS01120_A19 5', mRNA sequence
Length = 872
Score = 176 bits (379), Expect(2) = 1e-071
Identities = 69/86 (80%), Positives = 80/86 (93%)
Frame = -3 / +2
Query: 261 GIRKPKILGVREHVFTGSVSSLAWFMSAQETSFVTLGQRVLADPLKVRMHYGHPDVFDRL 82
G+R P ILG+REH+FTGSVSSLAWFMS QETSFVT+GQR+LA+PLKVR HYGHPDVFDRL
Sbjct: 299 GVRNPSILGLREHIFTGSVSSLAWFMSNQETSFVTIGQRLLANPLKVRFHYGHPDVFDRL 478
Query: 81 WFLGRGGISKASRIINISEDIFAGFN 4
+ L RGG+SKAS++IN+SEDIFAGFN
Sbjct: 479 FHLTRGGVSKASKVINLSEDIFAGFN 556
Score = 113 bits (242), Expect(2) = 1e-071
Identities = 44/56 (78%), Positives = 50/56 (89%)
Frame = -3 / +2
Query: 441 IYRVKLPGELKIGEGKPENQNHALIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEF 274
IYR+KLPG +GEGKPENQNHA+IFTRG+ +Q IDMNQDNY EEALKMRNLL+EF
Sbjct: 116 IYRIKLPGPAILGEGKPENQNHAIIFTRGEGLQAIDMNQDNYMEEALKMRNLLQEF 283
Score = 81.7 bits (172), Expect(2) = 3e-025
Identities = 43/76 (56%), Positives = 50/76 (65%)
Frame = +2 / -3
Query: 2 QLKPAKMSSLMLIILDALLIPPRPKNQRRSKTSGWP*CILTLSGSARTRCPRVTKLVSWA 181
+LKPA +SSL LI L+ALL PP K + S TSG P* TL G A + P VTKLVS
Sbjct: 558 ELKPANISSLRLITLEALLTPPLVK*KSLSNTSG*P*WNRTLRGLANSL*PIVTKLVS*F 379
Query: 182 DMNQAREETEPVKTCS 229
D+N AR+ET PV CS
Sbjct: 378 DINHARDETLPVNICS 331
Score = 53.3 bits (110), Expect(2) = 3e-025
Identities = 19/38 (50%), Positives = 24/38 (63%)
Frame = +1 / -2
Query: 289 VSHL*SFFKVVVLVHINSLNCIAPCEDECMILIFWLSF 402
+ H FF VV+LVHI L + CED M+L+FW SF
Sbjct: 268 IPHFQGFFHVVILVHIYCLQAFSTCEDNSMVLVFWFSF 155
>gb|DT493588.1|DT493588 WS0111.BR_N04 PT-P-FL-A-2 Populus trichocarpa cDNA clone WS0111_N04
5', mRNA sequence
Length = 599
Score = 176 bits (379), Expect(2) = 1e-071
Identities = 69/86 (80%), Positives = 80/86 (93%)
Frame = -3 / +2
Query: 261 GIRKPKILGVREHVFTGSVSSLAWFMSAQETSFVTLGQRVLADPLKVRMHYGHPDVFDRL 82
G+R P ILG+REH+FTGSVSSLAWFMS QETSFVT+GQR+LA+PLKVR HYGHPDVFDRL
Sbjct: 299 GVRNPSILGLREHIFTGSVSSLAWFMSNQETSFVTIGQRLLANPLKVRFHYGHPDVFDRL 478
Query: 81 WFLGRGGISKASRIINISEDIFAGFN 4
+ L RGG+SKAS++IN+SEDIFAGFN
Sbjct: 479 FHLTRGGVSKASKVINLSEDIFAGFN 556
Score = 113 bits (242), Expect(2) = 1e-071
Identities = 44/56 (78%), Positives = 50/56 (89%)
Frame = -3 / +2
Query: 441 IYRVKLPGELKIGEGKPENQNHALIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEF 274
IYR+KLPG +GEGKPENQNHA+IFTRG+ +Q IDMNQDNY EEALKMRNLL+EF
Sbjct: 116 IYRIKLPGPAILGEGKPENQNHAIIFTRGEGLQAIDMNQDNYMEEALKMRNLLQEF 283
Score = 81.7 bits (172), Expect(2) = 3e-025
Identities = 43/76 (56%), Positives = 50/76 (65%)
Frame = +2 / -3
Query: 2 QLKPAKMSSLMLIILDALLIPPRPKNQRRSKTSGWP*CILTLSGSARTRCPRVTKLVSWA 181
+LKPA +SSL LI L+ALL PP K + S TSG P* TL G A + P VTKLVS
Sbjct: 558 ELKPANISSLRLITLEALLTPPLVK*KSLSNTSG*P*WNRTLRGLANSL*PIVTKLVS*F 379
Query: 182 DMNQAREETEPVKTCS 229
D+N AR+ET PV CS
Sbjct: 378 DINHARDETLPVNICS 331
Score = 53.3 bits (110), Expect(2) = 3e-025
Identities = 19/38 (50%), Positives = 24/38 (63%)
Frame = +1 / -2
Query: 289 VSHL*SFFKVVVLVHINSLNCIAPCEDECMILIFWLSF 402
+ H FF VV+LVHI L + CED M+L+FW SF
Sbjct: 268 IPHFQGFFHVVILVHIYCLQAFSTCEDNSMVLVFWFSF 155
>gb|AC149540.1| Populus trichocarpa clone Pop1-111J19, complete sequence
Length = 96953
Score = 85.4 bits (180), Expect(4) = 7e-057
Identities = 33/40 (82%), Positives = 37/40 (92%)
Frame = -3 / +1
Query: 444 EIYRVKLPGELKIGEGKPENQNHALIFTRGDAVQTIDMNQ 325
EIY VKLPG K+GEGKPENQNHA+IFTRG+A+QTIDMNQ
Sbjct: 22474 EIYSVKLPGNPKLGEGKPENQNHAIIFTRGNAIQTIDMNQ 22593
Score = 80.3 bits (169), Expect(4) = 7e-057
Identities = 30/39 (76%), Positives = 37/39 (94%)
Frame = -3 / +3
Query: 126 KVRMHYGHPDVFDRLWFLGRGGISKASRIINISEDIFAG 10
+VRMHYGHPDVFDR++ + RGGISKASR+INISEDI++G
Sbjct: 25269 RVRMHYGHPDVFDRVFHITRGGISKASRVINISEDIYSG 25385
Score = 79.0 bits (166), Expect(4) = 7e-057
Identities = 32/38 (84%), Positives = 35/38 (92%)
Frame = -3 / +3
Query: 324 DNYFEEALKMRNLLEEFNRYYGIRKPKILGVREHVFTG 211
DNYFEEALKMRNLLEEF++ +GIR P ILGVREHVFTG
Sbjct: 24900 DNYFEEALKMRNLLEEFHQDHGIRPPTILGVREHVFTG 25013
Score = 56.5 bits (117), Expect(4) = 7e-057
Identities = 26/29 (89%), Positives = 27/29 (93%)
Frame = -3 / +3
Query: 210 SVSSLAWFMSAQETSFVTLGQRVLADPLK 124
SVSSLA FMS QETSFVTLGQRVLA+PLK
Sbjct: 25098 SVSSLASFMSNQETSFVTLGQRVLANPLK 25184
Score = 51.0 bits (105), Expect(4) = 4e-021
Identities = 25/39 (64%), Positives = 28/39 (71%)
Frame = +2 / -2
Query: 11 PAKMSSLMLIILDALLIPPRPKNQRRSKTSGWP*CILTL 127
P +SSLMLI +ALLIPPR + SKTSG P*CI TL
Sbjct: 25384 PEYISSLMLITREALLIPPRVMWKTLSKTSGCP*CIRTL 25268
Score = 48.7 bits (100), Expect(4) = 4e-021
Identities = 19/40 (47%), Positives = 26/40 (65%)
Frame = +1 / -3
Query: 325 LVHINSLNCIAPCEDECMILIFWLSFANLQLSRQLNPIDF 444
L+HIN LNCI+ + CM+LI SF+ + RQLN + F
Sbjct: 22593 LIHINCLNCISTSKYNCMVLILRFSFSKFWIPRQLNRVYF 22474
Score = 43.7 bits (89), Expect(4) = 4e-021
Identities = 19/26 (73%), Positives = 22/26 (84%)
Frame = +2 / -2
Query: 131 GSARTRCPRVTKLVSWADMNQAREET 208
G ARTR PRVTKLVS D+N+A+EET
Sbjct: 25177 GLARTRWPRVTKLVS*FDINEAKEET 25100
Score = 35.4 bits (71), Expect(4) = 4e-021
Identities = 16/17 (94%), Positives = 17/17 (100%)
Frame = +2 / -2
Query: 275 NSSSRFLIFRASSK*LS 325
NSSSRFLIF+ASSK*LS
Sbjct: 24949 NSSSRFLIFKASSK*LS 24899
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 300,114,631
Number of Sequences: 369679
Number of extensions: 4067313
Number of successful extensions: 40670
Number of sequences better than 1.0e-020: 5
length of database: 67,802,888
effective HSP length: 51
effective length of database: 48,949,259
effective search space used: 7782932181
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)