TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCG16f05.yg.2.1
(585 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU870838.1|BU870838 Q019A06 Populus flower cDNA library ... 88 2e-034
gb|DN496803.1|DN496803 Q019A06.5pR Populus dormant bud cDNA... 90 1e-032
>gb|BU870838.1|BU870838 Q019A06 Populus flower cDNA library Populus trichocarpa cDNA 5
prime, mRNA sequence
Length = 684
Score = 87.7 bits (185), Expect(4) = 2e-034
Identities = 43/111 (38%), Positives = 57/111 (51%)
Frame = -1 / +3
Query: 585 VNKXXXXXXXXVLXXXGDXGXXXLGXXXAIQXKNXXLFEDFNSDSTKRHSLTAVADSLKN 406
VN VL G+ G LG A Q K F + + SL VA+++
Sbjct: 69 VNNKKLVSGDAVLFLRGEDGELRLGVRTAAQVKCGPTFPALWNQHLNQSSLADVANAISM 248
Query: 405 RSVFHISYNPRATASEYIIPYAKFLKSLNHPVCIGARINFQCHNEDVSERR 253
RS F I YNPRA++SE+IIP+ KFLKSL+ G R+ + ED +ERR
Sbjct: 249 RSAFRIYYNPRASSSEFIIPFNKFLKSLDQSFSSGMRVKMRFETEDAAERR 401
Score = 58.8 bits (122), Expect(4) = 2e-034
Identities = 19/31 (61%), Positives = 26/31 (83%)
Frame = -1 / +3
Query: 258 RRSGVVVRISEIDPMKWPGSKWRSLLVRWED 166
R +G++ ISE+DP +WPGSKW+ LLVRW+D
Sbjct: 399 RYTGLITGISELDPTRWPGSKWKCLLVRWDD 491
Score = 29.0 bits (57), Expect(4) = 2e-034
Identities = 10/17 (58%), Positives = 11/17 (64%)
Frame = -1 / +3
Query: 168 DGAECNGQDRVSPWEIE 118
D E N RVSPWE+E
Sbjct: 486 DDMEANTHSRVSPWEVE 536
Score = 25.4 bits (49), Expect(4) = 2e-034
Identities = 12/36 (33%), Positives = 18/36 (50%)
Frame = -3 / +2
Query: 112 WWFCLCFSFPFCI*F*KNQVVSSGKFGRSSNVEWEW 5
+WF F + + F + QV + GR SN W+W
Sbjct: 551 FWFYF*FQYLYGTWFEEKQVWIAFIEGRISNP*WDW 658
>gb|DN496803.1|DN496803 Q019A06.5pR Populus dormant bud cDNA library Populus tremula cDNA
clone Q019A06 5', mRNA sequence
Length = 651
Score = 89.5 bits (189), Expect(3) = 1e-032
Identities = 44/111 (39%), Positives = 57/111 (51%)
Frame = -1 / +3
Query: 585 VNKXXXXXXXXVLXXXGDXGXXXLGXXXAIQXKNXXLFEDFNSDSTKRHSLTAVADSLKN 406
VNK VL G+ G LG A Q K F + SL VA+++
Sbjct: 93 VNKKKLVSGDAVLFLRGEDGELRLGVRRAAQVKCGPTFPALWNQQLNHSSLADVANAISM 272
Query: 405 RSVFHISYNPRATASEYIIPYAKFLKSLNHPVCIGARINFQCHNEDVSERR 253
RS F I YNPRA++SE+IIP+ KFLKSL+ G R+ + ED +ERR
Sbjct: 273 RSAFRIYYNPRASSSEFIIPFNKFLKSLDQSFSSGMRVKMRFETEDAAERR 425
Score = 58.8 bits (122), Expect(3) = 1e-032
Identities = 19/31 (61%), Positives = 26/31 (83%)
Frame = -1 / +3
Query: 258 RRSGVVVRISEIDPMKWPGSKWRSLLVRWED 166
R +G++ ISE+DP +WPGSKW+ LLVRW+D
Sbjct: 423 RYTGLITGISELDPTRWPGSKWKCLLVRWDD 515
Score = 29.0 bits (57), Expect(3) = 1e-032
Identities = 10/17 (58%), Positives = 11/17 (64%)
Frame = -1 / +3
Query: 168 DGAECNGQDRVSPWEIE 118
D E N RVSPWE+E
Sbjct: 510 DDMEANRHSRVSPWEVE 560
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 258,910,033
Number of Sequences: 369679
Number of extensions: 3504215
Number of successful extensions: 48128
Number of sequences better than 1.0e-020: 2
length of database: 67,802,888
effective HSP length: 51
effective length of database: 48,949,259
effective search space used: 6999744037
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)