TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCA17a05.yg.2.1
(548 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CK088334.1|CK088334 A048P19.3pR Hybrid aspen plasmid lib... 176 9e-044
gb|CV232041.1|CV232041 WS0196.B21_F15 PT-DX-N-A-10 Populus ... 176 9e-044
gb|CV277661.1|CV277661 WS0144.B21_D09 PTxD-IL-A-5 Populus t... 157 4e-038
gb|BI071270.1|BI071270 C054P51U Populus strain T89 leaves P... 147 3e-036
gb|CV237411.1|CV237411 WS01227.B21.1_N22 PT-GT-FL-A-3 Popul... 144 3e-034
gb|AJ774176.1|AJ774176 AJ774176 Populus euphratica shoot 3-... 137 2e-032
>gb|CK088334.1|CK088334 A048P19.3pR Hybrid aspen plasmid library Populus tremula x Populus
tremuloides cDNA clone A048P19 3', mRNA sequence
Length = 803
Score = 176 bits (379), Expect = 9e-044
Identities = 63/90 (70%), Positives = 78/90 (86%)
Frame = -3 / -1
Query: 546 VISAGIARDVASRHTNHSLRLFKMEDVSMGMWVEDYNASAPVQYVHSWRFCQFGCVDNYF 367
VIS IA+ V ++H SLRLFKMEDVSMGMWVE +N+S PVQY H+W+FCQ+GC++NY+
Sbjct: 548 VISTDIAKFVIAQHGKQSLRLFKMEDVSMGMWVEQFNSSTPVQYSHNWKFCQYGCMENYY 369
Query: 366 TAHYQSPRQMLCLWEKLSAGRAHCCNYR*H 277
TAHYQSPRQM+CLW+KL+ GRA CCN+R*H
Sbjct: 368 TAHYQSPRQMICLWDKLARGRAQCCNFR*H 279
>gb|CV232041.1|CV232041 WS0196.B21_F15 PT-DX-N-A-10 Populus trichocarpa cDNA clone
WS0196_F15 3', mRNA sequence
Length = 663
Score = 176 bits (379), Expect = 9e-044
Identities = 63/90 (70%), Positives = 78/90 (86%)
Frame = -3 / -2
Query: 546 VISAGIARDVASRHTNHSLRLFKMEDVSMGMWVEDYNASAPVQYVHSWRFCQFGCVDNYF 367
VIS IA+ V ++H SLRLFKMEDVSMGMWVE +N+S PVQY H+W+FCQ+GC++NY+
Sbjct: 602 VISTDIAKFVIAQHGKQSLRLFKMEDVSMGMWVEQFNSSTPVQYSHNWKFCQYGCMENYY 423
Query: 366 TAHYQSPRQMLCLWEKLSAGRAHCCNYR*H 277
TAHYQSPRQM+CLW+KL+ GRA CCN+R*H
Sbjct: 422 TAHYQSPRQMICLWDKLARGRAQCCNFR*H 333
>gb|CV277661.1|CV277661 WS0144.B21_D09 PTxD-IL-A-5 Populus trichocarpa x Populus deltoides
cDNA clone WS0144_D09 3', mRNA sequence
Length = 877
Score = 157 bits (338), Expect = 4e-038
Identities = 55/89 (61%), Positives = 73/89 (82%)
Frame = -3 / -3
Query: 546 VISAGIARDVASRHTNHSLRLFKMEDVSMGMWVEDYNASAPVQYVHSWRFCQFGCVDNYF 367
+IS +A + + +H LRLFKMEDVSMGMWVE +N S PV+Y+HS +FCQFGC+++Y+
Sbjct: 644 IISCDVADFIVAEFESHKLRLFKMEDVSMGMWVEKFNNSKPVEYIHSLKFCQFGCIEDYY 465
Query: 366 TAHYQSPRQMLCLWEKLSAGRAHCCNYR* 280
TAHYQSPRQM+CLW+KL+ G++ CCN R*
Sbjct: 464 TAHYQSPRQMICLWDKLNQGKSQCCNMR* 378
>gb|BI071270.1|BI071270 C054P51U Populus strain T89 leaves Populus tremula x Populus
tremuloides cDNA, mRNA sequence
Length = 340
Score = 147 bits (315), Expect(2) = 3e-036
Identities = 52/77 (67%), Positives = 65/77 (84%)
Frame = -3 / +2
Query: 546 VISAGIARDVASRHTNHSLRLFKMEDVSMGMWVEDYNASAPVQYVHSWRFCQFGCVDNYF 367
++S+ I R + S NH LRLFKMEDVSMGMWVE +N+S PV+YVHS +FCQFGC++ Y+
Sbjct: 38 ILSSDIGRFIVSEFENHKLRLFKMEDVSMGMWVEQFNSSRPVEYVHSLKFCQFGCIEGYY 217
Query: 366 TAHYQSPRQMLCLWEKL 316
TAHYQSP+QM+CLWEKL
Sbjct: 218 TAHYQSPKQMICLWEKL 268
Score = 23.5 bits (45), Expect(2) = 3e-036
Identities = 8/11 (72%), Positives = 9/11 (81%)
Frame = -3 / +1
Query: 312 AGRAHCCNYR* 280
AG+A CCN R*
Sbjct: 274 AGKAQCCNMR* 306
>gb|CV237411.1|CV237411 WS01227.B21.1_N22 PT-GT-FL-A-3 Populus trichocarpa cDNA clone
WS01227_N22 3', mRNA sequence
Length = 867
Score = 144 bits (310), Expect = 3e-034
Identities = 51/77 (66%), Positives = 65/77 (84%)
Frame = -3 / -3
Query: 546 VISAGIARDVASRHTNHSLRLFKMEDVSMGMWVEDYNASAPVQYVHSWRFCQFGCVDNYF 367
++S+ I R + S +H LRLFKMEDVSMGMWVE +N+S PV+YVHS +FCQFGC++ Y+
Sbjct: 637 ILSSDIGRFIVSEFESHKLRLFKMEDVSMGMWVEQFNSSRPVEYVHSLKFCQFGCIEGYY 458
Query: 366 TAHYQSPRQMLCLWEKL 316
TAHYQSP+QM+CLWEKL
Sbjct: 457 TAHYQSPKQMICLWEKL 407
>gb|AJ774176.1|AJ774176 AJ774176 Populus euphratica shoot 3-6 months Populus euphratica
cDNA clone P0000300013F04F1, mRNA sequence
Length = 366
Score = 137 bits (293), Expect(2) = 2e-032
Identities = 48/77 (62%), Positives = 63/77 (81%)
Frame = -3 / +1
Query: 546 VISAGIARDVASRHTNHSLRLFKMEDVSMGMWVEDYNASAPVQYVHSWRFCQFGCVDNYF 367
++S+ IA + S +H L+LFKMEDVSMGMWVE +N+S PV+YVHS +FCQ GC++ Y+
Sbjct: 40 ILSSDIACFIVSEFESHKLKLFKMEDVSMGMWVEQFNSSIPVEYVHSLKFCQLGCIEGYY 219
Query: 366 TAHYQSPRQMLCLWEKL 316
T HYQ+PRQM+CLWEKL
Sbjct: 220 TGHYQAPRQMICLWEKL 270
Score = 20.8 bits (39), Expect(2) = 2e-032
Identities = 6/7 (85%), Positives = 6/7 (85%)
Frame = -3 / +1
Query: 309 GRAHCCN 289
GRA CCN
Sbjct: 280 GRAQCCN 300
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 292,463,604
Number of Sequences: 369679
Number of extensions: 4394208
Number of successful extensions: 65078
Number of sequences better than 1.0e-020: 6
length of database: 67,802,888
effective HSP length: 51
effective length of database: 48,949,259
effective search space used: 6412352929
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)