TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBTB.060L08F020610.3.1
(677 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DT474803.1|DT474803 WS0125.BR_A19 PT-GT-FL-A-3 Populus t... 213 1e-054
gb|AC149484.1| Populus trichocarpa clone Pop1-69M19, comple... 142 4e-044
gb|DN501581.1|DN501581 V006H08.5pR Populus male catkins cDN... 160 1e-038
gb|AJ774822.1|AJ774822 AJ774822 Populus euphratica shoot 3-... 143 1e-033
>gb|DT474803.1|DT474803 WS0125.BR_A19 PT-GT-FL-A-3 Populus trichocarpa cDNA clone
WS0125_A19 5', mRNA sequence
Length = 585
Score = 213 bits (459), Expect = 1e-054
Identities = 81/106 (76%), Positives = 93/106 (87%)
Frame = +1 / +3
Query: 163 NARKIPDKMFGIFFEEINHAGAGGLWAELVSNRGFEAGGPNTPSNIDPWLIIGDESSIIV 342
+ R IP+ +FGIFFEEINHAGAGG+WAELV+NRGFEAGG NTPSNI PW IIGD+SS+IV
Sbjct: 267 STRPIPETLFGIFFEEINHAGAGGIWAELVNNRGFEAGGQNTPSNIAPWSIIGDQSSLIV 446
Query: 343 GTDRTSCFERNPVALRMEVLCGSKKTNACPSGGVGVYNPGYWGMNI 480
TDR+SCFERN VALRM+VLC S N CP+GGVG+YNPG+WGMNI
Sbjct: 447 STDRSSCFERNKVALRMDVLCDSTGGNICPAGGVGIYNPGFWGMNI 584
Score = 112 bits (239), Expect = 2e-024
Identities = 51/77 (66%), Positives = 56/77 (72%)
Frame = -1 / -3
Query: 404 QRTSIRSATGLRSKHDVRSVPTMILDSSPMISHGSIFEGVLGPPASNPLLLTSSAHNPPA 225
Q TSIR AT RSK + RSV T+ D SPMI G+IFEGV PPAS P L TSSAH PPA
Sbjct: 508 QSTSIRRATLFRSKQEERSVDTIKDD*SPMIDQGAIFEGVFWPPASKPRLFTSSAHIPPA 329
Query: 224 PAWLISSKKMPNILSGI 174
PAW ISSKK+P +SGI
Sbjct: 328 PAWFISSKKIPKSVSGI 278
>gb|AC149484.1| Populus trichocarpa clone Pop1-69M19, complete sequence
Length = 96930
Score = 142 bits (304), Expect(5) = 4e-044
Identities = 53/71 (74%), Positives = 61/71 (85%)
Frame = +1 / -1
Query: 262 GFEAGGPNTPSNIDPWLIIGDESSIIVGTDRTSCFERNPVALRMEVLCGSKKTNACPSGG 441
GFEAGG NTPSNI PW IIGD+SS+ V TDR+SCFERN VALRM VLC S+ +N CP+GG
Sbjct: 72342 GFEAGGQNTPSNIAPWSIIGDQSSLTVSTDRSSCFERNKVALRMHVLCDSEGSNICPAGG 72163
Query: 442 VGVYNPGYWGM 474
VG+YNPG+WGM
Sbjct: 72162 VGIYNPGFWGM 72130
Score = 52.4 bits (108), Expect(5) = 4e-044
Identities = 19/22 (86%), Positives = 21/22 (95%)
Frame = +1 / -3
Query: 199 FFEEINHAGAGGLWAELVSNRG 264
F +EINHAGAGG+WAELVSNRG
Sbjct: 72742 FLQEINHAGAGGIWAELVSNRG 72677
Score = 30.9 bits (61), Expect(5) = 4e-044
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = +2 / -1
Query: 509 CMLGHQMHCP*LLPLTSSDGLQKARCSYHH 598
C L HCP +L + S G+ ++C+Y H
Sbjct: 50385 CQL*LHAHCPTILSIMVS*GIYGSQCAYQH 50296
Score = 29.9 bits (59), Expect(5) = 4e-044
Identities = 11/15 (73%), Positives = 14/15 (93%)
Frame = +1 / -3
Query: 475 NIEKGKVYKVSLHVR 519
NIE+GK+YKV L+VR
Sbjct: 72022 NIEQGKIYKVVLYVR 71978
Score = 26.7 bits (52), Expect(5) = 4e-044
Identities = 9/15 (60%), Positives = 12/15 (80%)
Frame = +1 / -2
Query: 163 NARKIPDKMFGIFFE 207
+ R IP+ +FGIFFE
Sbjct: 72908 SGRPIPETLFGIFFE 72864
>gb|DN501581.1|DN501581 V006H08.5pR Populus male catkins cDNA library Populus trichocarpa
cDNA clone V006H08 5', mRNA sequence
Length = 508
Score = 160 bits (343), Expect = 1e-038
Identities = 61/81 (75%), Positives = 72/81 (88%)
Frame = +1 / +2
Query: 163 NARKIPDKMFGIFFEEINHAGAGGLWAELVSNRGFEAGGPNTPSNIDPWLIIGDESSIIV 342
+ R IP+ +FGIFFEEINHAGAGG+WAELV+NRGFE+GG NTPSNI PW +IGD+SS+IV
Sbjct: 266 STRPIPETLFGIFFEEINHAGAGGIWAELVNNRGFESGGQNTPSNIAPWSLIGDQSSLIV 445
Query: 343 GTDRTSCFERNPVALRMEVLC 405
TDR+SCFERN ALRM+VLC
Sbjct: 446 STDRSSCFERNNAALRMDVLC 508
Score = 104 bits (221), Expect = 7e-022
Identities = 48/77 (62%), Positives = 53/77 (68%)
Frame = -1 / -2
Query: 404 QRTSIRSATGLRSKHDVRSVPTMILDSSPMISHGSIFEGVLGPPASNPLLLTSSAHNPPA 225
Q TSIR A RSK + RSV T+ D SPM G+IFEGV PP S P L TSSAH PPA
Sbjct: 507 QSTSIRRAALFRSKQEERSVDTIKDD*SPMRDQGAIFEGVFWPPDSKPRLFTSSAHIPPA 328
Query: 224 PAWLISSKKMPNILSGI 174
PAW ISSKK+P +SGI
Sbjct: 327 PAWFISSKKIPKSVSGI 277
>gb|AJ774822.1|AJ774822 AJ774822 Populus euphratica shoot 3-6 months Populus euphratica
cDNA clone P0000300024F09F1, mRNA sequence
Length = 538
Score = 143 bits (307), Expect = 1e-033
Identities = 55/70 (78%), Positives = 63/70 (90%)
Frame = +1 / +2
Query: 163 NARKIPDKMFGIFFEEINHAGAGGLWAELVSNRGFEAGGPNTPSNIDPWLIIGDESSIIV 342
+ R IP+ +FGIFFEEINHAGAGG+WAELV+NRGFEAGG NTPSNI PW IIGD+SS+IV
Sbjct: 329 STRPIPETLFGIFFEEINHAGAGGIWAELVNNRGFEAGGQNTPSNIAPWSIIGDQSSLIV 508
Query: 343 GTDRTSCFER 372
TDR+SCFER
Sbjct: 509 STDRSSCFER 538
Score = 100 bits (213), Expect = 1e-020
Identities = 44/66 (66%), Positives = 49/66 (74%)
Frame = -1 / -2
Query: 371 RSKHDVRSVPTMILDSSPMISHGSIFEGVLGPPASNPLLLTSSAHNPPAPAWLISSKKMP 192
RSK + RSV T+ D SPMI G+IFEGV PPAS P L TSSAH PPAPAW ISSKK+P
Sbjct: 537 RSKQEERSVDTIKDD*SPMIDQGAIFEGVFWPPASKPRLFTSSAHIPPAPAWFISSKKIP 358
Query: 191 NILSGI 174
+SGI
Sbjct: 357 KSVSGI 340
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 368,901,346
Number of Sequences: 369679
Number of extensions: 5530299
Number of successful extensions: 58867
Number of sequences better than 1.0e-020: 4
length of database: 67,802,888
effective HSP length: 51
effective length of database: 48,949,259
effective search space used: 8517171066
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)