TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBL17f07.xg.2.1
(657 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DT472225.1|DT472225 WS01225.BR.1_O10 PT-GT-FL-A-3 Populu... 73 5e-025
gb|DV462960.1|DV462960 MTUNUL1.P1.H06 NUL Populus fremontii... 73 5e-025
gb|BU875945.1|BU875945 V013D11 Populus flower cDNA library ... 73 5e-025
gb|BU880539.1|BU880539 UM51TD09 Populus flower cDNA library... 72 1e-024
gb|BU825949.1|BU825949 UK114TH12 Populus apical shoot cDNA ... 70 3e-024
gb|BU826983.1|BU826983 UK127TB01 Populus apical shoot cDNA ... 70 1e-021
>gb|DT472225.1|DT472225 WS01225.BR.1_O10 PT-GT-FL-A-3 Populus trichocarpa cDNA clone
WS01225_O10 5', mRNA sequence
Length = 884
Score = 73.0 bits (153), Expect(3) = 5e-025
Identities = 28/78 (35%), Positives = 46/78 (58%)
Frame = +3 / +3
Query: 66 RYMDIRRDFHMAEGEGEWSYSKNCRRQQVAVXXTRPMVETAVKQVYAALLPRTMVVADLG 245
R M++ + HM G GE SY++N Q+ + T + E A+ +Y P ++ +ADLG
Sbjct: 18 RGMEVAQVLHMNGGRGETSYAQNSLVQRKVISITMRIAEEAITNIYCNTFPTSLAIADLG 197
Query: 246 CSAGPNTLLFISSVLSSI 299
CS+GPNTL +S ++ +
Sbjct: 198 CSSGPNTLYAVSELVKVV 251
Score = 39.6 bits (80), Expect(3) = 5e-025
Identities = 16/39 (41%), Positives = 22/39 (56%)
Frame = +3 / +3
Query: 477 PYYVMGLPESYYNRLFPRQSVHLFXSSYXXQXRXQEPEG 593
P + G+P S+Y RLF +S+H SSY + PEG
Sbjct: 399 PCFFAGVPGSFYCRLFRAKSLHFVHSSYSLMWLSRVPEG 515
Score = 39.1 bits (79), Expect(3) = 5e-025
Identities = 14/21 (66%), Positives = 18/21 (85%)
Frame = +3 / +3
Query: 366 ELQFVLNDLPGNDFNHLFRSV 428
E Q +LNDLPGNDFN +F+S+
Sbjct: 288 EYQVLLNDLPGNDFNAIFKSL 350
>gb|DV462960.1|DV462960 MTUNUL1.P1.H06 NUL Populus fremontii x Populus angustifolia cDNA,
mRNA sequence
Length = 785
Score = 73.0 bits (153), Expect(3) = 5e-025
Identities = 28/78 (35%), Positives = 46/78 (58%)
Frame = +3 / +3
Query: 66 RYMDIRRDFHMAEGEGEWSYSKNCRRQQVAVXXTRPMVETAVKQVYAALLPRTMVVADLG 245
R M++ + HM G GE SY++N Q+ + T + E A+ +Y P ++ +ADLG
Sbjct: 60 RGMEVAQVLHMNGGRGETSYAQNSLVQRKVISITMRIAEEAITNIYCNTFPTSLAIADLG 239
Query: 246 CSAGPNTLLFISSVLSSI 299
CS+GPNTL +S ++ +
Sbjct: 240 CSSGPNTLYAVSELVKVV 293
Score = 39.6 bits (80), Expect(3) = 5e-025
Identities = 16/39 (41%), Positives = 22/39 (56%)
Frame = +3 / +3
Query: 477 PYYVMGLPESYYNRLFPRQSVHLFXSSYXXQXRXQEPEG 593
P + G+P S+Y RLF +S+H SSY + PEG
Sbjct: 441 PCFFAGVPGSFYCRLFRAKSLHFVHSSYSLMWLSRVPEG 557
Score = 39.1 bits (79), Expect(3) = 5e-025
Identities = 14/21 (66%), Positives = 18/21 (85%)
Frame = +3 / +3
Query: 366 ELQFVLNDLPGNDFNHLFRSV 428
E Q +LNDLPGNDFN +F+S+
Sbjct: 330 EYQVLLNDLPGNDFNAIFKSL 392
>gb|BU875945.1|BU875945 V013D11 Populus flower cDNA library Populus trichocarpa cDNA 5
prime, mRNA sequence
Length = 595
Score = 73.0 bits (153), Expect(3) = 5e-025
Identities = 28/78 (35%), Positives = 46/78 (58%)
Frame = +3 / +1
Query: 66 RYMDIRRDFHMAEGEGEWSYSKNCRRQQVAVXXTRPMVETAVKQVYAALLPRTMVVADLG 245
R M++ + HM G GE SY++N Q+ + T + E A+ +Y P ++ +ADLG
Sbjct: 28 RGMEVAQVLHMNGGRGETSYAQNSLVQRKVISITMRIAEEAITNIYCNTFPTSLAIADLG 207
Query: 246 CSAGPNTLLFISSVLSSI 299
CS+GPNTL +S ++ +
Sbjct: 208 CSSGPNTLYAVSELVKVV 261
Score = 39.6 bits (80), Expect(3) = 5e-025
Identities = 16/39 (41%), Positives = 22/39 (56%)
Frame = +3 / +1
Query: 477 PYYVMGLPESYYNRLFPRQSVHLFXSSYXXQXRXQEPEG 593
P + G+P S+Y RLF +S+H SSY + PEG
Sbjct: 409 PCFFAGVPGSFYCRLFRAKSLHFVHSSYSLMWLSRVPEG 525
Score = 39.1 bits (79), Expect(3) = 5e-025
Identities = 14/21 (66%), Positives = 18/21 (85%)
Frame = +3 / +1
Query: 366 ELQFVLNDLPGNDFNHLFRSV 428
E Q +LNDLPGNDFN +F+S+
Sbjct: 298 EYQVLLNDLPGNDFNAIFKSL 360
>gb|BU880539.1|BU880539 UM51TD09 Populus flower cDNA library Populus trichocarpa cDNA 5
prime, mRNA sequence
Length = 551
Score = 72.1 bits (151), Expect(3) = 1e-024
Identities = 27/76 (35%), Positives = 45/76 (59%)
Frame = +3 / +3
Query: 72 MDIRRDFHMAEGEGEWSYSKNCRRQQVAVXXTRPMVETAVKQVYAALLPRTMVVADLGCS 251
M++ + HM G GE SY++N Q+ + T + E A+ +Y P ++ +ADLGCS
Sbjct: 3 MEVAQVLHMNGGRGETSYAQNSLVQRKVISITMRIAEEAITNIYCNTFPTSLAIADLGCS 182
Query: 252 AGPNTLLFISSVLSSI 299
+GPNTL +S ++ +
Sbjct: 183 SGPNTLYAVSELVKVV 230
Score = 39.6 bits (80), Expect(3) = 1e-024
Identities = 16/39 (41%), Positives = 22/39 (56%)
Frame = +3 / +3
Query: 477 PYYVMGLPESYYNRLFPRQSVHLFXSSYXXQXRXQEPEG 593
P + G+P S+Y RLF +S+H SSY + PEG
Sbjct: 378 PCFFAGVPGSFYCRLFRAKSLHFVHSSYSLMWLSRVPEG 494
Score = 39.1 bits (79), Expect(3) = 1e-024
Identities = 14/21 (66%), Positives = 18/21 (85%)
Frame = +3 / +3
Query: 366 ELQFVLNDLPGNDFNHLFRSV 428
E Q +LNDLPGNDFN +F+S+
Sbjct: 267 EYQVLLNDLPGNDFNAIFKSL 329
>gb|BU825949.1|BU825949 UK114TH12 Populus apical shoot cDNA library Populus tremula x
Populus tremuloides cDNA 5 prime, mRNA sequence
Length = 499
Score = 70.3 bits (147), Expect(3) = 3e-024
Identities = 26/76 (34%), Positives = 46/76 (60%)
Frame = +3 / +2
Query: 72 MDIRRDFHMAEGEGEWSYSKNCRRQQVAVXXTRPMVETAVKQVYAALLPRTMVVADLGCS 251
M++ + HM G GE SY++N Q+ + T + + A+ +Y + P ++ +ADLGCS
Sbjct: 2 MEVAQVLHMNGGRGEKSYAENSLVQRKVISITMRIAKEAITNLYCSTFPTSLAIADLGCS 181
Query: 252 AGPNTLLFISSVLSSI 299
+GPNTL +S ++ +
Sbjct: 182 SGPNTLYAVSELVKVV 229
Score = 39.6 bits (80), Expect(3) = 3e-024
Identities = 16/39 (41%), Positives = 22/39 (56%)
Frame = +3 / +2
Query: 477 PYYVMGLPESYYNRLFPRQSVHLFXSSYXXQXRXQEPEG 593
P + G+P S+Y RLF +S+H SSY + PEG
Sbjct: 377 PCFFAGVPGSFYCRLFRAKSLHFVHSSYSLMWLSRVPEG 493
Score = 39.1 bits (79), Expect(3) = 3e-024
Identities = 14/21 (66%), Positives = 18/21 (85%)
Frame = +3 / +2
Query: 366 ELQFVLNDLPGNDFNHLFRSV 428
E Q +LNDLPGNDFN +F+S+
Sbjct: 266 EYQVLLNDLPGNDFNAIFKSL 328
>gb|BU826983.1|BU826983 UK127TB01 Populus apical shoot cDNA library Populus tremula x
Populus tremuloides cDNA 5 prime, mRNA sequence
Length = 481
Score = 70.3 bits (147), Expect(3) = 1e-021
Identities = 26/76 (34%), Positives = 46/76 (60%)
Frame = +3 / +2
Query: 72 MDIRRDFHMAEGEGEWSYSKNCRRQQVAVXXTRPMVETAVKQVYAALLPRTMVVADLGCS 251
M++ + HM G GE SY++N Q+ + T + + A+ +Y + P ++ +ADLGCS
Sbjct: 2 MEVAQVLHMNGGRGEKSYAENSLVQRKVISITMRIAKEAITNLYCSTFPTSLAIADLGCS 181
Query: 252 AGPNTLLFISSVLSSI 299
+GPNTL +S ++ +
Sbjct: 182 SGPNTLYAVSELVKVV 229
Score = 39.1 bits (79), Expect(3) = 1e-021
Identities = 14/21 (66%), Positives = 18/21 (85%)
Frame = +3 / +2
Query: 366 ELQFVLNDLPGNDFNHLFRSV 428
E Q +LNDLPGNDFN +F+S+
Sbjct: 266 EYQVLLNDLPGNDFNAIFKSL 328
Score = 30.9 bits (61), Expect(3) = 1e-021
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = +3 / +2
Query: 477 PYYVMGLPESYYNRLFPRQSVHLFXSS 557
P + G+P S+Y RLF +S+H SS
Sbjct: 377 PCFFAGVPGSFYCRLFRAKSLHFVHSS 457
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 278,460,948
Number of Sequences: 369679
Number of extensions: 3399056
Number of successful extensions: 38752
Number of sequences better than 1.0e-020: 6
length of database: 67,802,888
effective HSP length: 51
effective length of database: 48,949,259
effective search space used: 8174526253
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)